Experiment: Spectinomycin 0.05 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_4349 and Pf1N1B4_4350 are separated by 89 nucleotides Pf1N1B4_4350 and Pf1N1B4_4351 overlap by 4 nucleotides Pf1N1B4_4351 and Pf1N1B4_4352 overlap by 4 nucleotides  
        
        Pf1N1B4_4349 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 4,805,007 to 4,806,104 
         
        _4349 
         
        
        Pf1N1B4_4350 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 4,806,194 to 4,807,078 
         
        _4350 
         
        
        Pf1N1B4_4351 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 4,807,075 to 4,807,989 
         
        _4351 
         
        
        Pf1N1B4_4352 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 4,807,986 to 4,809,128 
         
        _4352 
         Position (kb)  
4807 
 
4808 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 4806.127 kb on + strand at 4806.128 kb on - strand at 4806.139 kb on + strand at 4806.139 kb on + strand at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.797 kb on - strand, within Pf1N1B4_4350 at 4806.874 kb on - strand, within Pf1N1B4_4350 at 4806.940 kb on - strand, within Pf1N1B4_4350 at 4806.940 kb on - strand, within Pf1N1B4_4350 at 4807.024 kb on - strand at 4807.024 kb on - strand at 4807.028 kb on - strand at 4807.445 kb on + strand, within Pf1N1B4_4351 at 4807.446 kb on - strand, within Pf1N1B4_4351 at 4807.446 kb on - strand, within Pf1N1B4_4351 at 4807.452 kb on - strand, within Pf1N1B4_4351 at 4807.452 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.467 kb on - strand, within Pf1N1B4_4351 at 4807.664 kb on + strand, within Pf1N1B4_4351 at 4807.665 kb on - strand, within Pf1N1B4_4351 at 4807.665 kb on - strand at 4807.745 kb on + strand, within Pf1N1B4_4351 at 4807.745 kb on + strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.746 kb on - strand, within Pf1N1B4_4351 at 4807.773 kb on - strand, within Pf1N1B4_4351 at 4807.820 kb on + strand, within Pf1N1B4_4351 at 4807.832 kb on + strand, within Pf1N1B4_4351 at 4807.841 kb on - strand at 4808.029 kb on + strand at 4808.030 kb on - strand at 4808.177 kb on + strand, within Pf1N1B4_4352 at 4808.233 kb on + strand, within Pf1N1B4_4352 at 4808.234 kb on - strand, within Pf1N1B4_4352 at 4808.234 kb on - strand, within Pf1N1B4_4352 at 4808.407 kb on + strand, within Pf1N1B4_4352 at 4808.407 kb on + strand, within Pf1N1B4_4352 at 4808.408 kb on - strand, within Pf1N1B4_4352 at 4808.408 kb on - strand, within Pf1N1B4_4352  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Spectinomycin 0.05 mg/ml             remove   4,806,127   +        -1.5   4,806,128   -        -0.9   4,806,139   +        -0.2   4,806,139   +        -0.5   4,806,633   +    Pf1N1B4_4350   0.50  +0.1   4,806,633   +    Pf1N1B4_4350   0.50  -1.1   4,806,633   +    Pf1N1B4_4350   0.50  +1.2   4,806,633   +    Pf1N1B4_4350   0.50  +1.0   4,806,633   +    Pf1N1B4_4350   0.50  -0.6   4,806,634   -    Pf1N1B4_4350   0.50  +0.9   4,806,634   -    Pf1N1B4_4350   0.50  +1.2   4,806,634   -    Pf1N1B4_4350   0.50  +1.1   4,806,634   -    Pf1N1B4_4350   0.50  -2.6   4,806,634   -    Pf1N1B4_4350   0.50  -1.1   4,806,797   -    Pf1N1B4_4350   0.68  +2.1   4,806,874   -    Pf1N1B4_4350   0.77  +1.0   4,806,940   -    Pf1N1B4_4350   0.84  -0.2   4,806,940   -    Pf1N1B4_4350   0.84  -0.5   4,807,024   -        -0.3   4,807,024   -        +0.8   4,807,028   -        +0.4   4,807,445   +    Pf1N1B4_4351   0.40  -1.1   4,807,446   -    Pf1N1B4_4351   0.41  -0.2   4,807,446   -    Pf1N1B4_4351   0.41  -0.3   4,807,452   -    Pf1N1B4_4351   0.41  +0.3   4,807,452   -    Pf1N1B4_4351   0.41  -0.1   4,807,467   -    Pf1N1B4_4351   0.43  +1.2   4,807,467   -    Pf1N1B4_4351   0.43  +0.3   4,807,467   -    Pf1N1B4_4351   0.43  -0.8   4,807,664   +    Pf1N1B4_4351   0.64  -1.4   4,807,665   -    Pf1N1B4_4351   0.64  +0.1   4,807,665   -        -1.1   4,807,745   +    Pf1N1B4_4351   0.73  +1.3   4,807,745   +    Pf1N1B4_4351   0.73  -2.1   4,807,746   -    Pf1N1B4_4351   0.73  +0.7   4,807,746   -    Pf1N1B4_4351   0.73  +0.3   4,807,746   -    Pf1N1B4_4351   0.73  -1.8   4,807,746   -    Pf1N1B4_4351   0.73  +0.8   4,807,773   -    Pf1N1B4_4351   0.76  -0.7   4,807,820   +    Pf1N1B4_4351   0.81  -0.8   4,807,832   +    Pf1N1B4_4351   0.83  -0.1   4,807,841   -        +0.5   4,808,029   +        -0.6   4,808,030   -        +0.5   4,808,177   +    Pf1N1B4_4352   0.17  -1.2   4,808,233   +    Pf1N1B4_4352   0.22  +0.0   4,808,234   -    Pf1N1B4_4352   0.22  +1.3   4,808,234   -    Pf1N1B4_4352   0.22  +0.8   4,808,407   +    Pf1N1B4_4352   0.37  -1.5   4,808,407   +    Pf1N1B4_4352   0.37  -0.8   4,808,408   -    Pf1N1B4_4352   0.37  +1.3   4,808,408   -    Pf1N1B4_4352   0.37  +0.1 
 
Or see this region's nucleotide sequence