Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4348 and Pf1N1B4_4349 are separated by 406 nucleotides Pf1N1B4_4349 and Pf1N1B4_4350 are separated by 89 nucleotides Pf1N1B4_4350 and Pf1N1B4_4351 overlap by 4 nucleotides
Pf1N1B4_4348 - 2'-5' RNA ligase, at 4,804,061 to 4,804,600
_4348
Pf1N1B4_4349 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 4,805,007 to 4,806,104
_4349
Pf1N1B4_4350 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 4,806,194 to 4,807,078
_4350
Pf1N1B4_4351 - Putrescine transport system permease protein PotH (TC 3.A.1.11.2), at 4,807,075 to 4,807,989
_4351
Position (kb)
4805
4806
4807 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4804.389 kb on - strand, within Pf1N1B4_4348 at 4804.534 kb on + strand, within Pf1N1B4_4348 at 4804.535 kb on - strand, within Pf1N1B4_4348 at 4804.535 kb on - strand, within Pf1N1B4_4348 at 4804.535 kb on - strand at 4804.598 kb on + strand at 4804.598 kb on + strand at 4804.599 kb on - strand at 4804.871 kb on + strand at 4804.872 kb on - strand at 4804.872 kb on - strand at 4804.872 kb on - strand at 4804.872 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4804.947 kb on - strand at 4805.012 kb on + strand at 4805.083 kb on + strand at 4805.083 kb on + strand at 4805.083 kb on + strand at 4805.084 kb on - strand at 4805.084 kb on - strand at 4805.084 kb on - strand at 4805.084 kb on - strand at 4805.084 kb on - strand at 4805.242 kb on + strand at 4805.390 kb on - strand, within Pf1N1B4_4349 at 4805.434 kb on + strand, within Pf1N1B4_4349 at 4805.435 kb on - strand, within Pf1N1B4_4349 at 4805.435 kb on - strand, within Pf1N1B4_4349 at 4805.453 kb on - strand, within Pf1N1B4_4349 at 4805.720 kb on - strand, within Pf1N1B4_4349 at 4805.720 kb on - strand, within Pf1N1B4_4349 at 4805.824 kb on + strand at 4805.825 kb on - strand, within Pf1N1B4_4349 at 4805.924 kb on + strand, within Pf1N1B4_4349 at 4805.942 kb on - strand at 4805.998 kb on + strand at 4806.066 kb on + strand at 4806.067 kb on - strand at 4806.127 kb on + strand at 4806.128 kb on - strand at 4806.139 kb on + strand at 4806.139 kb on + strand at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.633 kb on + strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.634 kb on - strand, within Pf1N1B4_4350 at 4806.797 kb on - strand, within Pf1N1B4_4350 at 4806.874 kb on - strand, within Pf1N1B4_4350 at 4806.940 kb on - strand, within Pf1N1B4_4350 at 4806.940 kb on - strand, within Pf1N1B4_4350 at 4807.024 kb on - strand at 4807.024 kb on - strand at 4807.028 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,804,389 - Pf1N1B4_4348 0.61 +0.1 4,804,534 + Pf1N1B4_4348 0.88 -1.3 4,804,535 - Pf1N1B4_4348 0.88 -0.0 4,804,535 - Pf1N1B4_4348 0.88 +1.1 4,804,535 - -1.0 4,804,598 + +0.3 4,804,598 + -0.2 4,804,599 - -0.0 4,804,871 + -1.0 4,804,872 - +0.7 4,804,872 - -0.2 4,804,872 - +0.4 4,804,872 - -1.5 4,804,947 - -1.5 4,804,947 - -1.9 4,804,947 - +0.3 4,804,947 - +0.9 4,804,947 - -2.0 4,804,947 - -1.2 4,805,012 + +1.1 4,805,083 + +0.5 4,805,083 + +0.2 4,805,083 + -0.9 4,805,084 - -1.5 4,805,084 - -0.8 4,805,084 - +0.1 4,805,084 - +0.2 4,805,084 - -1.5 4,805,242 + +0.5 4,805,390 - Pf1N1B4_4349 0.35 -0.7 4,805,434 + Pf1N1B4_4349 0.39 +1.1 4,805,435 - Pf1N1B4_4349 0.39 +0.1 4,805,435 - Pf1N1B4_4349 0.39 +0.3 4,805,453 - Pf1N1B4_4349 0.41 -0.5 4,805,720 - Pf1N1B4_4349 0.65 +0.8 4,805,720 - Pf1N1B4_4349 0.65 -0.5 4,805,824 + -0.2 4,805,825 - Pf1N1B4_4349 0.74 +0.4 4,805,924 + Pf1N1B4_4349 0.84 -0.5 4,805,942 - +2.1 4,805,998 + +0.5 4,806,066 + -1.2 4,806,067 - -0.0 4,806,127 + -1.5 4,806,128 - -0.9 4,806,139 + -0.2 4,806,139 + -0.5 4,806,633 + Pf1N1B4_4350 0.50 +0.1 4,806,633 + Pf1N1B4_4350 0.50 -1.1 4,806,633 + Pf1N1B4_4350 0.50 +1.2 4,806,633 + Pf1N1B4_4350 0.50 +1.0 4,806,633 + Pf1N1B4_4350 0.50 -0.6 4,806,634 - Pf1N1B4_4350 0.50 +0.9 4,806,634 - Pf1N1B4_4350 0.50 +1.2 4,806,634 - Pf1N1B4_4350 0.50 +1.1 4,806,634 - Pf1N1B4_4350 0.50 -2.6 4,806,634 - Pf1N1B4_4350 0.50 -1.1 4,806,797 - Pf1N1B4_4350 0.68 +2.1 4,806,874 - Pf1N1B4_4350 0.77 +1.0 4,806,940 - Pf1N1B4_4350 0.84 -0.2 4,806,940 - Pf1N1B4_4350 0.84 -0.5 4,807,024 - -0.3 4,807,024 - +0.8 4,807,028 - +0.4
Or see this region's nucleotide sequence