Experiment: Spectinomycin 0.05 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_4317 and Pf1N1B4_4318 are separated by 147 nucleotides Pf1N1B4_4318 and Pf1N1B4_4319 are separated by 576 nucleotides 
        Pf1N1B4_4317 - DNA/RNA non-specific endonuclease, at 4,778,421 to 4,779,251 
        _4317 
         
        
        Pf1N1B4_4318 - hypothetical protein, at 4,779,399 to 4,780,760 
        _4318 
         
        
        Pf1N1B4_4319 - Phosphonate ABC transporter permease protein phnE1 (TC 3.A.1.9.1), at 4,781,337 to 4,782,104 
        _4319 
         Position (kb) 4779 
4780 
4781 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 4778.701 kb on + strand, within Pf1N1B4_4317 at 4779.129 kb on + strand, within Pf1N1B4_4317 at 4779.129 kb on + strand, within Pf1N1B4_4317 at 4779.133 kb on + strand at 4779.133 kb on + strand at 4779.175 kb on - strand at 4779.175 kb on - strand at 4779.317 kb on - strand at 4779.743 kb on - strand at 4779.918 kb on - strand, within Pf1N1B4_4318 at 4779.924 kb on - strand, within Pf1N1B4_4318 at 4780.096 kb on + strand, within Pf1N1B4_4318 at 4780.096 kb on + strand, within Pf1N1B4_4318 at 4780.097 kb on - strand, within Pf1N1B4_4318 at 4780.226 kb on - strand, within Pf1N1B4_4318 at 4780.260 kb on - strand, within Pf1N1B4_4318 at 4780.613 kb on - strand, within Pf1N1B4_4318 at 4780.839 kb on - strand at 4780.898 kb on - strand at 4781.111 kb on + strand at 4781.112 kb on - strand at 4781.112 kb on - strand at 4781.149 kb on + strand at 4781.149 kb on + strand at 4781.156 kb on + strand at 4781.223 kb on + strand at 4781.223 kb on + strand at 4781.223 kb on + strand at 4781.223 kb on + strand at 4781.224 kb on - strand at 4781.224 kb on - strand at 4781.224 kb on - strand at 4781.280 kb on + strand at 4781.458 kb on + strand, within Pf1N1B4_4319 at 4781.458 kb on + strand, within Pf1N1B4_4319 at 4781.458 kb on + strand, within Pf1N1B4_4319 at 4781.458 kb on + strand, within Pf1N1B4_4319 at 4781.459 kb on - strand, within Pf1N1B4_4319 at 4781.543 kb on - strand, within Pf1N1B4_4319 at 4781.543 kb on - strand, within Pf1N1B4_4319 at 4781.550 kb on + strand, within Pf1N1B4_4319 at 4781.581 kb on + strand, within Pf1N1B4_4319 at 4781.596 kb on - strand, within Pf1N1B4_4319  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Spectinomycin 0.05 mg/ml remove 4,778,701 +  Pf1N1B4_4317 0.34  -0.8 4,779,129 +  Pf1N1B4_4317 0.85  -0.2 4,779,129 +  Pf1N1B4_4317 0.85  +1.7 4,779,133 +  +2.6 4,779,133 +  +0.6 4,779,175 -  +2.6 4,779,175 -  +0.1 4,779,317 -  -0.2 4,779,743 -  +0.6 4,779,918 -  Pf1N1B4_4318 0.38  -0.3 4,779,924 -  Pf1N1B4_4318 0.39  +0.1 4,780,096 +  Pf1N1B4_4318 0.51  -2.0 4,780,096 +  Pf1N1B4_4318 0.51  -0.7 4,780,097 -  Pf1N1B4_4318 0.51  -0.8 4,780,226 -  Pf1N1B4_4318 0.61  -2.0 4,780,260 -  Pf1N1B4_4318 0.63  -1.4 4,780,613 -  Pf1N1B4_4318 0.89  -0.4 4,780,839 -  +0.1 4,780,898 -  -0.4 4,781,111 +  -0.0 4,781,112 -  +0.1 4,781,112 -  +1.6 4,781,149 +  +0.7 4,781,149 +  -0.0 4,781,156 +  +0.4 4,781,223 +  -0.9 4,781,223 +  -0.8 4,781,223 +  -0.0 4,781,223 +  +0.6 4,781,224 -  +0.1 4,781,224 -  -1.0 4,781,224 -  +0.4 4,781,280 +  +0.1 4,781,458 +  Pf1N1B4_4319 0.16  +0.1 4,781,458 +  Pf1N1B4_4319 0.16  -1.4 4,781,458 +  Pf1N1B4_4319 0.16  -0.0 4,781,458 +  Pf1N1B4_4319 0.16  +0.1 4,781,459 -  Pf1N1B4_4319 0.16  -2.3 4,781,543 -  Pf1N1B4_4319 0.27  -0.2 4,781,543 -  Pf1N1B4_4319 0.27  -2.0 4,781,550 +  Pf1N1B4_4319 0.28  -0.1 4,781,581 +  Pf1N1B4_4319 0.32  -0.7 4,781,596 -  Pf1N1B4_4319 0.34  -0.4 
Or see this region's nucleotide sequence