Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4248

Experiment: Spectinomycin 0.05 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.05 mg/ml
4,709,500	+		Pf1N1B4_4247	0.61	+0.6
4,709,500	+		Pf1N1B4_4247	0.61	+1.8
4,709,500	+		Pf1N1B4_4247	0.61	-0.6
4,709,501	-		Pf1N1B4_4247	0.61	+0.8
4,709,798	+		Pf1N1B4_4247	0.87	-0.4
4,709,798	+		Pf1N1B4_4247	0.87	-0.6
4,709,798	+		Pf1N1B4_4247	0.87	+0.6
4,709,798	+		Pf1N1B4_4247	0.87	+0.6
4,709,799	-		Pf1N1B4_4247	0.88	-1.6
4,709,799	-		Pf1N1B4_4247	0.88	-0.1
4,709,986	+				+1.0
4,710,094	+				+1.2
4,710,320	-		Pf1N1B4_4248	0.20	-0.7
4,710,544	+		Pf1N1B4_4248	0.40	-0.4
4,710,593	-				+1.0
4,710,823	+		Pf1N1B4_4248	0.66	+0.2
4,711,090	+		Pf1N1B4_4248	0.90	+0.6
4,711,097	-				-0.2
4,711,551	+		Pf1N1B4_4249	0.31	+1.1
4,711,551	+		Pf1N1B4_4249	0.31	-0.1
4,711,650	+		Pf1N1B4_4249	0.43	-0.6
4,711,650	+		Pf1N1B4_4249	0.43	-0.6
4,711,650	+		Pf1N1B4_4249	0.43	-0.6
4,711,650	+		Pf1N1B4_4249	0.43	-1.4
4,711,653	+		Pf1N1B4_4249	0.44	-0.3
4,711,653	+		Pf1N1B4_4249	0.44	-0.1
4,711,653	+		Pf1N1B4_4249	0.44	+2.1
4,711,662	+				+0.6
4,711,663	-		Pf1N1B4_4249	0.45	-0.2
4,711,663	-		Pf1N1B4_4249	0.45	-1.0
4,711,985	+		Pf1N1B4_4249	0.87	-2.2
4,711,985	+		Pf1N1B4_4249	0.87	+1.3
4,712,036	+				+0.7
4,712,036	+				+0.6
4,712,036	+				-1.2
4,712,037	-				-1.4
4,712,037	-				-1.0
4,712,123	+				+0.6
4,712,123	+				+1.3
4,712,123	+				+1.0
4,712,123	+				-1.1
4,712,150	-				-2.0