Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4213 and Pf1N1B4_4214 are separated by 106 nucleotides Pf1N1B4_4214 and Pf1N1B4_4215 are separated by 456 nucleotides
Pf1N1B4_4213 - Potassium channel protein, at 4,666,941 to 4,667,816
_4213
Pf1N1B4_4214 - Arginine/ornithine antiporter ArcD, at 4,667,923 to 4,669,389
_4214
Pf1N1B4_4215 - Adenylate cyclase (EC 4.6.1.1), at 4,669,846 to 4,671,435
_4215
Position (kb)
4667
4668
4669
4670 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4667.049 kb on - strand, within Pf1N1B4_4213 at 4667.049 kb on - strand, within Pf1N1B4_4213 at 4667.094 kb on + strand, within Pf1N1B4_4213 at 4667.094 kb on + strand, within Pf1N1B4_4213 at 4667.188 kb on - strand, within Pf1N1B4_4213 at 4667.188 kb on - strand, within Pf1N1B4_4213 at 4667.194 kb on - strand, within Pf1N1B4_4213 at 4667.197 kb on + strand, within Pf1N1B4_4213 at 4667.198 kb on - strand, within Pf1N1B4_4213 at 4667.198 kb on - strand, within Pf1N1B4_4213 at 4667.249 kb on + strand, within Pf1N1B4_4213 at 4667.249 kb on + strand, within Pf1N1B4_4213 at 4667.251 kb on + strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.261 kb on + strand, within Pf1N1B4_4213 at 4667.433 kb on + strand, within Pf1N1B4_4213 at 4667.434 kb on - strand, within Pf1N1B4_4213 at 4667.436 kb on - strand, within Pf1N1B4_4213 at 4667.475 kb on + strand, within Pf1N1B4_4213 at 4667.499 kb on - strand at 4667.653 kb on - strand, within Pf1N1B4_4213 at 4667.653 kb on - strand, within Pf1N1B4_4213 at 4667.696 kb on + strand, within Pf1N1B4_4213 at 4667.696 kb on + strand, within Pf1N1B4_4213 at 4667.757 kb on + strand at 4667.757 kb on + strand at 4667.758 kb on - strand at 4667.945 kb on + strand at 4667.945 kb on + strand at 4667.974 kb on + strand at 4667.974 kb on + strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4668.072 kb on - strand, within Pf1N1B4_4214 at 4668.113 kb on + strand, within Pf1N1B4_4214 at 4668.124 kb on + strand, within Pf1N1B4_4214 at 4668.132 kb on - strand at 4668.191 kb on + strand, within Pf1N1B4_4214 at 4668.192 kb on - strand at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.283 kb on + strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.313 kb on - strand, within Pf1N1B4_4214 at 4668.350 kb on - strand, within Pf1N1B4_4214 at 4668.620 kb on - strand, within Pf1N1B4_4214 at 4668.620 kb on - strand, within Pf1N1B4_4214 at 4668.718 kb on - strand at 4668.891 kb on + strand, within Pf1N1B4_4214 at 4669.127 kb on + strand, within Pf1N1B4_4214 at 4669.128 kb on - strand, within Pf1N1B4_4214 at 4669.249 kb on - strand at 4669.249 kb on - strand at 4669.332 kb on + strand at 4669.333 kb on - strand at 4669.684 kb on + strand at 4669.685 kb on - strand at 4669.697 kb on + strand at 4669.698 kb on - strand at 4669.698 kb on - strand at 4669.699 kb on + strand at 4670.185 kb on + strand, within Pf1N1B4_4215
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,667,049 - Pf1N1B4_4213 0.12 -1.0 4,667,049 - Pf1N1B4_4213 0.12 +1.0 4,667,094 + Pf1N1B4_4213 0.17 -1.4 4,667,094 + Pf1N1B4_4213 0.17 -0.4 4,667,188 - Pf1N1B4_4213 0.28 -2.4 4,667,188 - Pf1N1B4_4213 0.28 +1.0 4,667,194 - Pf1N1B4_4213 0.29 +1.0 4,667,197 + Pf1N1B4_4213 0.29 -0.4 4,667,198 - Pf1N1B4_4213 0.29 -1.5 4,667,198 - Pf1N1B4_4213 0.29 -0.5 4,667,249 + Pf1N1B4_4213 0.35 +0.6 4,667,249 + Pf1N1B4_4213 0.35 -1.0 4,667,251 + Pf1N1B4_4213 0.35 -1.0 4,667,252 - Pf1N1B4_4213 0.36 -1.4 4,667,252 - Pf1N1B4_4213 0.36 +0.0 4,667,252 - Pf1N1B4_4213 0.36 +1.4 4,667,252 - Pf1N1B4_4213 0.36 +0.6 4,667,252 - Pf1N1B4_4213 0.36 +1.0 4,667,261 + Pf1N1B4_4213 0.37 -0.1 4,667,433 + Pf1N1B4_4213 0.56 +1.5 4,667,434 - Pf1N1B4_4213 0.56 -1.4 4,667,436 - Pf1N1B4_4213 0.57 -0.9 4,667,475 + Pf1N1B4_4213 0.61 +0.7 4,667,499 - -0.4 4,667,653 - Pf1N1B4_4213 0.81 -2.4 4,667,653 - Pf1N1B4_4213 0.81 +0.2 4,667,696 + Pf1N1B4_4213 0.86 +1.3 4,667,696 + Pf1N1B4_4213 0.86 +0.3 4,667,757 + +1.6 4,667,757 + -0.4 4,667,758 - -0.2 4,667,945 + -0.2 4,667,945 + -0.4 4,667,974 + -2.0 4,667,974 + -1.7 4,667,975 - -0.4 4,667,975 - -0.8 4,667,975 - +0.1 4,667,975 - +0.4 4,667,975 - +0.8 4,667,975 - -1.7 4,667,975 - -0.1 4,667,975 - -1.0 4,667,975 - +0.6 4,668,072 - Pf1N1B4_4214 0.10 -2.0 4,668,113 + Pf1N1B4_4214 0.13 +0.1 4,668,124 + Pf1N1B4_4214 0.14 -0.1 4,668,132 - +1.0 4,668,191 + Pf1N1B4_4214 0.18 +1.8 4,668,192 - +0.6 4,668,273 - Pf1N1B4_4214 0.24 +0.4 4,668,273 - Pf1N1B4_4214 0.24 -1.5 4,668,273 - Pf1N1B4_4214 0.24 -0.1 4,668,283 + Pf1N1B4_4214 0.25 +0.9 4,668,284 - Pf1N1B4_4214 0.25 +1.6 4,668,284 - Pf1N1B4_4214 0.25 +0.6 4,668,284 - Pf1N1B4_4214 0.25 +0.9 4,668,284 - Pf1N1B4_4214 0.25 -0.4 4,668,288 - Pf1N1B4_4214 0.25 +0.9 4,668,288 - Pf1N1B4_4214 0.25 +0.6 4,668,288 - Pf1N1B4_4214 0.25 +1.6 4,668,313 - Pf1N1B4_4214 0.27 -0.4 4,668,350 - Pf1N1B4_4214 0.29 -0.4 4,668,620 - Pf1N1B4_4214 0.48 +0.4 4,668,620 - Pf1N1B4_4214 0.48 +1.4 4,668,718 - +1.6 4,668,891 + Pf1N1B4_4214 0.66 +0.2 4,669,127 + Pf1N1B4_4214 0.82 +2.1 4,669,128 - Pf1N1B4_4214 0.82 -1.8 4,669,249 - -0.0 4,669,249 - +1.9 4,669,332 + +0.6 4,669,333 - -1.0 4,669,684 + +0.6 4,669,685 - +0.3 4,669,697 + +0.6 4,669,698 - -2.8 4,669,698 - -0.7 4,669,699 + +0.6 4,670,185 + Pf1N1B4_4215 0.21 +0.6
Or see this region's nucleotide sequence