Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4035 and Pf1N1B4_4036 overlap by 78 nucleotides Pf1N1B4_4036 and Pf1N1B4_4037 are separated by 328 nucleotides Pf1N1B4_4037 and Pf1N1B4_4038 overlap by 4 nucleotides Pf1N1B4_4038 and Pf1N1B4_4039 are separated by 2 nucleotides
Pf1N1B4_4035 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 4,485,531 to 4,487,990
_4035
Pf1N1B4_4036 - hypothetical protein, at 4,487,913 to 4,488,056
_4036
Pf1N1B4_4037 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 4,488,385 to 4,489,740
_4037
Pf1N1B4_4038 - NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3), at 4,489,737 to 4,490,234
_4038
Pf1N1B4_4039 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 4,490,237 to 4,492,021
_4039
Position (kb)
4488
4489
4490 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4487.468 kb on - strand, within Pf1N1B4_4035 at 4487.707 kb on + strand, within Pf1N1B4_4035 at 4487.708 kb on - strand, within Pf1N1B4_4035 at 4487.756 kb on - strand at 4487.765 kb on - strand at 4488.152 kb on + strand at 4488.239 kb on - strand at 4488.330 kb on - strand at 4488.379 kb on - strand at 4488.379 kb on - strand at 4488.379 kb on - strand at 4488.471 kb on - strand at 4488.782 kb on + strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4489.185 kb on - strand at 4489.310 kb on + strand, within Pf1N1B4_4037 at 4489.741 kb on - strand at 4489.741 kb on - strand at 4489.741 kb on - strand at 4489.745 kb on - strand at 4490.011 kb on + strand, within Pf1N1B4_4038 at 4490.011 kb on + strand, within Pf1N1B4_4038 at 4490.012 kb on - strand, within Pf1N1B4_4038 at 4490.273 kb on - strand at 4490.354 kb on - strand at 4490.404 kb on - strand at 4490.404 kb on - strand at 4490.563 kb on - strand, within Pf1N1B4_4039
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,487,468 - Pf1N1B4_4035 0.79 -0.3 4,487,707 + Pf1N1B4_4035 0.88 -1.9 4,487,708 - Pf1N1B4_4035 0.88 -1.3 4,487,756 - -1.0 4,487,765 - -0.3 4,488,152 + -0.1 4,488,239 - -0.5 4,488,330 - -0.8 4,488,379 - +2.0 4,488,379 - +1.3 4,488,379 - +1.5 4,488,471 - -0.3 4,488,782 + Pf1N1B4_4037 0.29 -0.6 4,488,783 - Pf1N1B4_4037 0.29 -1.6 4,488,783 - Pf1N1B4_4037 0.29 +0.2 4,489,185 - -1.6 4,489,310 + Pf1N1B4_4037 0.68 +0.7 4,489,741 - +1.0 4,489,741 - -0.9 4,489,741 - +0.0 4,489,745 - -0.9 4,490,011 + Pf1N1B4_4038 0.55 -1.3 4,490,011 + Pf1N1B4_4038 0.55 -0.2 4,490,012 - Pf1N1B4_4038 0.55 -1.8 4,490,273 - +1.9 4,490,354 - -0.0 4,490,404 - -0.6 4,490,404 - -0.3 4,490,563 - Pf1N1B4_4039 0.18 -0.0
Or see this region's nucleotide sequence