Experiment: Spectinomycin 0.05 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_4034 and Pf1N1B4_4035 overlap by 4 nucleotides Pf1N1B4_4035 and Pf1N1B4_4036 overlap by 78 nucleotides Pf1N1B4_4036 and Pf1N1B4_4037 are separated by 328 nucleotides  
        
        Pf1N1B4_4034 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 4,484,527 to 4,485,534 
         
        _4034 
         
        
        Pf1N1B4_4035 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 4,485,531 to 4,487,990 
         
        _4035 
         
        
        Pf1N1B4_4036 - hypothetical protein, at 4,487,913 to 4,488,056 
         
        _4036 
         
        
        Pf1N1B4_4037 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 4,488,385 to 4,489,740 
         
        _4037 
         Position (kb)  
4485 
 
4486 
 
4487 
 
4488 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 at 4484.807 kb on + strand, within Pf1N1B4_4034 at 4485.124 kb on - strand, within Pf1N1B4_4034 at 4485.503 kb on - strand at 4485.742 kb on + strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4485.743 kb on - strand at 4486.005 kb on + strand, within Pf1N1B4_4035 at 4487.245 kb on + strand, within Pf1N1B4_4035 at 4487.246 kb on - strand, within Pf1N1B4_4035 at 4487.468 kb on - strand, within Pf1N1B4_4035 at 4487.707 kb on + strand, within Pf1N1B4_4035 at 4487.708 kb on - strand, within Pf1N1B4_4035 at 4487.756 kb on - strand at 4487.765 kb on - strand at 4488.152 kb on + strand at 4488.239 kb on - strand at 4488.330 kb on - strand at 4488.379 kb on - strand at 4488.379 kb on - strand at 4488.379 kb on - strand at 4488.471 kb on - strand at 4488.782 kb on + strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Spectinomycin 0.05 mg/ml             remove   4,484,807   +    Pf1N1B4_4034   0.28  +0.1   4,485,124   -    Pf1N1B4_4034   0.59  -2.2   4,485,503   -        -1.3   4,485,742   +        +1.1   4,485,743   -        -1.9   4,485,743   -        -0.3   4,485,743   -        -1.6   4,485,743   -        -0.2   4,486,005   +    Pf1N1B4_4035   0.19  -0.3   4,487,245   +    Pf1N1B4_4035   0.70  +0.0   4,487,246   -    Pf1N1B4_4035   0.70  -0.5   4,487,468   -    Pf1N1B4_4035   0.79  -0.3   4,487,707   +    Pf1N1B4_4035   0.88  -1.9   4,487,708   -    Pf1N1B4_4035   0.88  -1.3   4,487,756   -        -1.0   4,487,765   -        -0.3   4,488,152   +        -0.1   4,488,239   -        -0.5   4,488,330   -        -0.8   4,488,379   -        +2.0   4,488,379   -        +1.3   4,488,379   -        +1.5   4,488,471   -        -0.3   4,488,782   +    Pf1N1B4_4037   0.29  -0.6   4,488,783   -    Pf1N1B4_4037   0.29  -1.6   4,488,783   -    Pf1N1B4_4037   0.29  +0.2 
 
Or see this region's nucleotide sequence