Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4034

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4032 and Pf1N1B4_4033 are separated by 9 nucleotidesPf1N1B4_4033 and Pf1N1B4_4034 are separated by 12 nucleotidesPf1N1B4_4034 and Pf1N1B4_4035 overlap by 4 nucleotides Pf1N1B4_4032 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 4,483,456 to 4,483,959 _4032 Pf1N1B4_4033 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 4,483,969 to 4,484,514 _4033 Pf1N1B4_4034 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 4,484,527 to 4,485,534 _4034 Pf1N1B4_4035 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 4,485,531 to 4,487,990 _4035 Position (kb) 4484 4485 4486Strain fitness (log2 ratio) -2 -1 0 1at 4483.634 kb on + strand, within Pf1N1B4_4032at 4483.635 kb on - strand, within Pf1N1B4_4032at 4484.060 kb on + strand, within Pf1N1B4_4033at 4484.061 kb on - strandat 4484.069 kb on + strand, within Pf1N1B4_4033at 4484.070 kb on - strand, within Pf1N1B4_4033at 4484.070 kb on - strand, within Pf1N1B4_4033at 4484.508 kb on - strandat 4484.508 kb on - strandat 4484.807 kb on + strand, within Pf1N1B4_4034at 4485.124 kb on - strand, within Pf1N1B4_4034at 4485.503 kb on - strandat 4485.742 kb on + strandat 4485.743 kb on - strandat 4485.743 kb on - strandat 4485.743 kb on - strandat 4485.743 kb on - strandat 4486.005 kb on + strand, within Pf1N1B4_4035

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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4,483,634 + Pf1N1B4_4032 0.35 +0.1
4,483,635 - Pf1N1B4_4032 0.36 +0.5
4,484,060 + Pf1N1B4_4033 0.17 -2.6
4,484,061 - +1.3
4,484,069 + Pf1N1B4_4033 0.18 -1.0
4,484,070 - Pf1N1B4_4033 0.18 +1.3
4,484,070 - Pf1N1B4_4033 0.18 -1.2
4,484,508 - -0.3
4,484,508 - -1.6
4,484,807 + Pf1N1B4_4034 0.28 +0.1
4,485,124 - Pf1N1B4_4034 0.59 -2.2
4,485,503 - -1.3
4,485,742 + +1.1
4,485,743 - -1.9
4,485,743 - -0.3
4,485,743 - -1.6
4,485,743 - -0.2
4,486,005 + Pf1N1B4_4035 0.19 -0.3

Or see this region's nucleotide sequence