Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4033

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4030 and Pf1N1B4_4031 overlap by 4 nucleotidesPf1N1B4_4031 and Pf1N1B4_4032 are separated by 2 nucleotidesPf1N1B4_4032 and Pf1N1B4_4033 are separated by 9 nucleotidesPf1N1B4_4033 and Pf1N1B4_4034 are separated by 12 nucleotides Pf1N1B4_4030 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 4,481,295 to 4,483,148 _4030 Pf1N1B4_4031 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 4,483,145 to 4,483,453 _4031 Pf1N1B4_4032 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 4,483,456 to 4,483,959 _4032 Pf1N1B4_4033 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 4,483,969 to 4,484,514 _4033 Pf1N1B4_4034 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 4,484,527 to 4,485,534 _4034 Position (kb) 4483 4484 4485Strain fitness (log2 ratio) -2 -1 0 1at 4483.005 kb on + strandat 4483.006 kb on - strandat 4483.198 kb on - strand, within Pf1N1B4_4031at 4483.634 kb on + strand, within Pf1N1B4_4032at 4483.635 kb on - strand, within Pf1N1B4_4032at 4484.060 kb on + strand, within Pf1N1B4_4033at 4484.061 kb on - strandat 4484.069 kb on + strand, within Pf1N1B4_4033at 4484.070 kb on - strand, within Pf1N1B4_4033at 4484.070 kb on - strand, within Pf1N1B4_4033at 4484.508 kb on - strandat 4484.508 kb on - strandat 4484.807 kb on + strand, within Pf1N1B4_4034at 4485.124 kb on - strand, within Pf1N1B4_4034at 4485.503 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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4,483,005 + -0.4
4,483,006 - +0.7
4,483,198 - Pf1N1B4_4031 0.17 -1.0
4,483,634 + Pf1N1B4_4032 0.35 +0.1
4,483,635 - Pf1N1B4_4032 0.36 +0.5
4,484,060 + Pf1N1B4_4033 0.17 -2.6
4,484,061 - +1.3
4,484,069 + Pf1N1B4_4033 0.18 -1.0
4,484,070 - Pf1N1B4_4033 0.18 +1.3
4,484,070 - Pf1N1B4_4033 0.18 -1.2
4,484,508 - -0.3
4,484,508 - -1.6
4,484,807 + Pf1N1B4_4034 0.28 +0.1
4,485,124 - Pf1N1B4_4034 0.59 -2.2
4,485,503 - -1.3

Or see this region's nucleotide sequence