Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4030 and Pf1N1B4_4031 overlap by 4 nucleotides Pf1N1B4_4031 and Pf1N1B4_4032 are separated by 2 nucleotides Pf1N1B4_4032 and Pf1N1B4_4033 are separated by 9 nucleotides Pf1N1B4_4033 and Pf1N1B4_4034 are separated by 12 nucleotides
Pf1N1B4_4030 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 4,481,295 to 4,483,148
_4030
Pf1N1B4_4031 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 4,483,145 to 4,483,453
_4031
Pf1N1B4_4032 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 4,483,456 to 4,483,959
_4032
Pf1N1B4_4033 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 4,483,969 to 4,484,514
_4033
Pf1N1B4_4034 - NADH-ubiquinone oxidoreductase chain H (EC 1.6.5.3), at 4,484,527 to 4,485,534
_4034
Position (kb)
4483
4484
4485 Strain fitness (log2 ratio)
-2
-1
0
1 at 4483.005 kb on + strand at 4483.006 kb on - strand at 4483.198 kb on - strand, within Pf1N1B4_4031 at 4483.634 kb on + strand, within Pf1N1B4_4032 at 4483.635 kb on - strand, within Pf1N1B4_4032 at 4484.060 kb on + strand, within Pf1N1B4_4033 at 4484.061 kb on - strand at 4484.069 kb on + strand, within Pf1N1B4_4033 at 4484.070 kb on - strand, within Pf1N1B4_4033 at 4484.070 kb on - strand, within Pf1N1B4_4033 at 4484.508 kb on - strand at 4484.508 kb on - strand at 4484.807 kb on + strand, within Pf1N1B4_4034 at 4485.124 kb on - strand, within Pf1N1B4_4034 at 4485.503 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,483,005 + -0.4 4,483,006 - +0.7 4,483,198 - Pf1N1B4_4031 0.17 -1.0 4,483,634 + Pf1N1B4_4032 0.35 +0.1 4,483,635 - Pf1N1B4_4032 0.36 +0.5 4,484,060 + Pf1N1B4_4033 0.17 -2.6 4,484,061 - +1.3 4,484,069 + Pf1N1B4_4033 0.18 -1.0 4,484,070 - Pf1N1B4_4033 0.18 +1.3 4,484,070 - Pf1N1B4_4033 0.18 -1.2 4,484,508 - -0.3 4,484,508 - -1.6 4,484,807 + Pf1N1B4_4034 0.28 +0.1 4,485,124 - Pf1N1B4_4034 0.59 -2.2 4,485,503 - -1.3
Or see this region's nucleotide sequence