Experiment: Spectinomycin 0.05 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_4029 and Pf1N1B4_4030 are separated by 39 nucleotides Pf1N1B4_4030 and Pf1N1B4_4031 overlap by 4 nucleotides Pf1N1B4_4031 and Pf1N1B4_4032 are separated by 2 nucleotides Pf1N1B4_4032 and Pf1N1B4_4033 are separated by 9 nucleotides  
        
        Pf1N1B4_4029 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 4,479,723 to 4,481,255 
         
        _4029 
         
        
        Pf1N1B4_4030 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 4,481,295 to 4,483,148 
         
        _4030 
         
        
        Pf1N1B4_4031 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 4,483,145 to 4,483,453 
         
        _4031 
         
        
        Pf1N1B4_4032 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 4,483,456 to 4,483,959 
         
        _4032 
         
        
        Pf1N1B4_4033 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 4,483,969 to 4,484,514 
         
        _4033 
         Position (kb)  
4481 
 
4482 
 
4483 
 
4484 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 at 4480.352 kb on - strand, within Pf1N1B4_4029 at 4480.444 kb on + strand, within Pf1N1B4_4029 at 4480.444 kb on + strand, within Pf1N1B4_4029 at 4480.588 kb on + strand, within Pf1N1B4_4029 at 4480.801 kb on + strand, within Pf1N1B4_4029 at 4480.802 kb on - strand, within Pf1N1B4_4029 at 4481.325 kb on + strand at 4481.861 kb on - strand, within Pf1N1B4_4030 at 4481.861 kb on - strand, within Pf1N1B4_4030 at 4481.954 kb on - strand, within Pf1N1B4_4030 at 4482.168 kb on + strand, within Pf1N1B4_4030 at 4482.215 kb on - strand, within Pf1N1B4_4030 at 4482.826 kb on + strand, within Pf1N1B4_4030 at 4482.923 kb on + strand, within Pf1N1B4_4030 at 4483.005 kb on + strand at 4483.006 kb on - strand at 4483.198 kb on - strand, within Pf1N1B4_4031 at 4483.634 kb on + strand, within Pf1N1B4_4032 at 4483.635 kb on - strand, within Pf1N1B4_4032 at 4484.060 kb on + strand, within Pf1N1B4_4033 at 4484.061 kb on - strand at 4484.069 kb on + strand, within Pf1N1B4_4033 at 4484.070 kb on - strand, within Pf1N1B4_4033 at 4484.070 kb on - strand, within Pf1N1B4_4033  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Spectinomycin 0.05 mg/ml             remove   4,480,352   -    Pf1N1B4_4029   0.41  -1.6   4,480,444   +    Pf1N1B4_4029   0.47  +0.4   4,480,444   +    Pf1N1B4_4029   0.47  +1.3   4,480,588   +    Pf1N1B4_4029   0.56  +0.5   4,480,801   +    Pf1N1B4_4029   0.70  -1.9   4,480,802   -    Pf1N1B4_4029   0.70  -1.1   4,481,325   +        -0.4   4,481,861   -    Pf1N1B4_4030   0.31  -0.9   4,481,861   -    Pf1N1B4_4030   0.31  -0.3   4,481,954   -    Pf1N1B4_4030   0.36  -0.3   4,482,168   +    Pf1N1B4_4030   0.47  -0.5   4,482,215   -    Pf1N1B4_4030   0.50  +1.0   4,482,826   +    Pf1N1B4_4030   0.83  -0.3   4,482,923   +    Pf1N1B4_4030   0.88  -0.6   4,483,005   +        -0.4   4,483,006   -        +0.7   4,483,198   -    Pf1N1B4_4031   0.17  -1.0   4,483,634   +    Pf1N1B4_4032   0.35  +0.1   4,483,635   -    Pf1N1B4_4032   0.36  +0.5   4,484,060   +    Pf1N1B4_4033   0.17  -2.6   4,484,061   -        +1.3   4,484,069   +    Pf1N1B4_4033   0.18  -1.0   4,484,070   -    Pf1N1B4_4033   0.18  +1.3   4,484,070   -    Pf1N1B4_4033   0.18  -1.2 
 
Or see this region's nucleotide sequence