Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_395 and Pf1N1B4_396 overlap by 4 nucleotides Pf1N1B4_396 and Pf1N1B4_397 are separated by 60 nucleotides Pf1N1B4_397 and Pf1N1B4_398 are separated by 196 nucleotides
Pf1N1B4_395 - Alcohol dehydrogenase (EC 1.1.1.1), at 464,129 to 465,181
_395
Pf1N1B4_396 - D-galactonate regulator, IclR family, at 465,178 to 465,981
_396
Pf1N1B4_397 - Hexuronate transporter, at 466,042 to 467,352
_397
Pf1N1B4_398 - Galactonate dehydratase (EC 4.2.1.6), at 467,549 to 468,697
_398
Position (kb)
466
467
468 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.113 kb on + strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.114 kb on - strand at 465.143 kb on + strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.144 kb on - strand at 465.180 kb on - strand at 465.266 kb on + strand, within Pf1N1B4_396 at 465.266 kb on + strand, within Pf1N1B4_396 at 465.266 kb on + strand, within Pf1N1B4_396 at 465.267 kb on - strand, within Pf1N1B4_396 at 465.267 kb on - strand, within Pf1N1B4_396 at 465.393 kb on - strand, within Pf1N1B4_396 at 465.393 kb on - strand, within Pf1N1B4_396 at 465.486 kb on - strand, within Pf1N1B4_396 at 465.617 kb on + strand, within Pf1N1B4_396 at 465.618 kb on - strand, within Pf1N1B4_396 at 465.767 kb on + strand, within Pf1N1B4_396 at 465.794 kb on + strand, within Pf1N1B4_396 at 465.794 kb on + strand, within Pf1N1B4_396 at 465.795 kb on - strand, within Pf1N1B4_396 at 465.835 kb on - strand, within Pf1N1B4_396 at 466.021 kb on - strand at 466.021 kb on - strand at 466.044 kb on - strand at 466.054 kb on - strand at 466.082 kb on + strand at 466.083 kb on - strand at 466.116 kb on + strand at 466.116 kb on + strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.117 kb on - strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.118 kb on + strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.119 kb on - strand at 466.123 kb on - strand at 466.179 kb on + strand, within Pf1N1B4_397 at 466.541 kb on + strand, within Pf1N1B4_397 at 466.542 kb on - strand, within Pf1N1B4_397 at 466.542 kb on - strand, within Pf1N1B4_397 at 466.601 kb on + strand, within Pf1N1B4_397 at 466.601 kb on + strand, within Pf1N1B4_397 at 466.602 kb on - strand, within Pf1N1B4_397 at 466.659 kb on + strand, within Pf1N1B4_397 at 466.660 kb on - strand, within Pf1N1B4_397 at 466.904 kb on + strand, within Pf1N1B4_397 at 466.904 kb on + strand, within Pf1N1B4_397 at 466.904 kb on + strand, within Pf1N1B4_397 at 466.904 kb on + strand, within Pf1N1B4_397 at 466.905 kb on - strand, within Pf1N1B4_397 at 466.905 kb on - strand, within Pf1N1B4_397 at 466.974 kb on - strand, within Pf1N1B4_397 at 467.085 kb on + strand at 467.087 kb on + strand, within Pf1N1B4_397 at 467.087 kb on + strand, within Pf1N1B4_397 at 467.088 kb on - strand, within Pf1N1B4_397 at 467.252 kb on + strand at 467.252 kb on + strand at 467.252 kb on + strand at 467.252 kb on + strand at 467.253 kb on - strand at 467.368 kb on + strand at 467.368 kb on + strand at 467.368 kb on + strand at 467.368 kb on + strand at 467.368 kb on + strand at 467.368 kb on + strand at 467.369 kb on - strand at 467.369 kb on - strand at 467.369 kb on - strand at 467.369 kb on - strand at 467.369 kb on - strand at 467.369 kb on - strand at 467.369 kb on - strand at 467.374 kb on + strand at 467.374 kb on + strand at 467.375 kb on - strand at 467.375 kb on - strand at 467.631 kb on + strand at 467.631 kb on + strand at 467.632 kb on - strand at 467.632 kb on - strand at 467.632 kb on - strand at 467.722 kb on - strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.748 kb on + strand, within Pf1N1B4_398 at 467.749 kb on - strand, within Pf1N1B4_398 at 467.749 kb on - strand, within Pf1N1B4_398 at 467.865 kb on + strand, within Pf1N1B4_398 at 467.866 kb on - strand, within Pf1N1B4_398 at 467.866 kb on - strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.946 kb on + strand, within Pf1N1B4_398 at 467.947 kb on - strand, within Pf1N1B4_398 at 468.091 kb on - strand, within Pf1N1B4_398 at 468.130 kb on + strand, within Pf1N1B4_398 at 468.348 kb on + strand, within Pf1N1B4_398 at 468.349 kb on - strand, within Pf1N1B4_398 at 468.349 kb on - strand, within Pf1N1B4_398
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 465,113 + +0.3 465,113 + -0.8 465,113 + -1.1 465,113 + +0.5 465,113 + -0.7 465,113 + +0.9 465,114 - -1.1 465,114 - -0.9 465,114 - -0.1 465,114 - +0.1 465,114 - -0.1 465,114 - -1.1 465,114 - -0.3 465,143 + -0.7 465,144 - +0.5 465,144 - +1.2 465,144 - +0.9 465,180 - -0.3 465,266 + Pf1N1B4_396 0.11 +0.7 465,266 + Pf1N1B4_396 0.11 -0.9 465,266 + Pf1N1B4_396 0.11 -0.7 465,267 - Pf1N1B4_396 0.11 -1.2 465,267 - Pf1N1B4_396 0.11 -1.0 465,393 - Pf1N1B4_396 0.27 -0.1 465,393 - Pf1N1B4_396 0.27 -0.7 465,486 - Pf1N1B4_396 0.38 +0.2 465,617 + Pf1N1B4_396 0.55 -0.0 465,618 - Pf1N1B4_396 0.55 -0.1 465,767 + Pf1N1B4_396 0.73 +0.1 465,794 + Pf1N1B4_396 0.77 -1.3 465,794 + Pf1N1B4_396 0.77 -0.1 465,795 - Pf1N1B4_396 0.77 +0.9 465,835 - Pf1N1B4_396 0.82 +0.9 466,021 - +1.5 466,021 - +1.9 466,044 - +0.6 466,054 - -0.4 466,082 + -1.4 466,083 - -1.7 466,116 + +0.5 466,116 + -0.3 466,117 - -0.8 466,117 - +0.3 466,117 - -0.9 466,117 - -0.7 466,117 - -0.7 466,117 - +1.2 466,118 + -0.7 466,118 + +0.0 466,118 + +0.0 466,118 + -0.4 466,118 + -1.4 466,119 - -0.7 466,119 - -0.9 466,119 - +1.2 466,119 - +0.9 466,119 - +0.1 466,119 - +0.2 466,119 - -1.7 466,119 - +0.4 466,119 - -0.1 466,119 - +1.3 466,119 - -0.1 466,119 - -2.7 466,119 - -1.7 466,119 - -1.7 466,119 - +0.5 466,119 - -1.1 466,119 - -3.2 466,119 - -2.3 466,119 - -0.4 466,119 - -1.4 466,119 - -1.8 466,119 - +0.3 466,123 - -0.1 466,179 + Pf1N1B4_397 0.10 -0.1 466,541 + Pf1N1B4_397 0.38 +0.1 466,542 - Pf1N1B4_397 0.38 +0.4 466,542 - Pf1N1B4_397 0.38 -1.6 466,601 + Pf1N1B4_397 0.43 -1.4 466,601 + Pf1N1B4_397 0.43 +0.9 466,602 - Pf1N1B4_397 0.43 -0.4 466,659 + Pf1N1B4_397 0.47 -2.6 466,660 - Pf1N1B4_397 0.47 +0.5 466,904 + Pf1N1B4_397 0.66 -0.1 466,904 + Pf1N1B4_397 0.66 -0.7 466,904 + Pf1N1B4_397 0.66 +0.9 466,904 + Pf1N1B4_397 0.66 +1.6 466,905 - Pf1N1B4_397 0.66 +0.9 466,905 - Pf1N1B4_397 0.66 -1.6 466,974 - Pf1N1B4_397 0.71 +0.4 467,085 + -1.1 467,087 + Pf1N1B4_397 0.80 +1.7 467,087 + Pf1N1B4_397 0.80 -0.7 467,088 - Pf1N1B4_397 0.80 -0.5 467,252 + +0.3 467,252 + -0.1 467,252 + -0.7 467,252 + -0.5 467,253 - +0.3 467,368 + +0.6 467,368 + +0.5 467,368 + +0.3 467,368 + +1.9 467,368 + +0.7 467,368 + +0.1 467,369 - -0.7 467,369 - -3.1 467,369 - -0.3 467,369 - +0.3 467,369 - +1.1 467,369 - +0.9 467,369 - -0.1 467,374 + -0.6 467,374 + -1.4 467,375 - -1.1 467,375 - -0.3 467,631 + -1.6 467,631 + +0.1 467,632 - -0.4 467,632 - -0.9 467,632 - +1.8 467,722 - Pf1N1B4_398 0.15 -0.7 467,748 + Pf1N1B4_398 0.17 -0.0 467,748 + Pf1N1B4_398 0.17 -0.5 467,748 + Pf1N1B4_398 0.17 -0.3 467,748 + Pf1N1B4_398 0.17 -0.4 467,749 - Pf1N1B4_398 0.17 -1.7 467,749 - Pf1N1B4_398 0.17 +0.5 467,865 + Pf1N1B4_398 0.28 +0.6 467,866 - Pf1N1B4_398 0.28 -0.1 467,866 - Pf1N1B4_398 0.28 +1.7 467,946 + Pf1N1B4_398 0.35 -0.4 467,946 + Pf1N1B4_398 0.35 -0.1 467,946 + Pf1N1B4_398 0.35 -0.1 467,947 - Pf1N1B4_398 0.35 -0.5 468,091 - Pf1N1B4_398 0.47 -0.3 468,130 + Pf1N1B4_398 0.51 -1.4 468,348 + Pf1N1B4_398 0.70 -1.1 468,349 - Pf1N1B4_398 0.70 -1.1 468,349 - Pf1N1B4_398 0.70 +0.3
Or see this region's nucleotide sequence