Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3818 and Pf1N1B4_3819 overlap by 4 nucleotides Pf1N1B4_3819 and Pf1N1B4_3820 are separated by 184 nucleotides
Pf1N1B4_3818 - Propionate catabolism operon transcriptional regulator of GntR family [predicted], at 4,229,543 to 4,230,265
_3818
Pf1N1B4_3819 - Methylisocitrate lyase (EC 4.1.3.30), at 4,230,262 to 4,231,152
_3819
Pf1N1B4_3820 - 2-methylcitrate synthase (EC 2.3.3.5), at 4,231,337 to 4,232,464
_3820
Position (kb)
4230
4231
4232 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4229.311 kb on + strand at 4229.311 kb on + strand at 4229.311 kb on + strand at 4229.311 kb on + strand at 4229.317 kb on + strand at 4229.318 kb on - strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.371 kb on + strand at 4229.385 kb on + strand at 4229.410 kb on + strand at 4229.412 kb on + strand at 4229.412 kb on + strand at 4229.412 kb on + strand at 4229.499 kb on + strand at 4229.542 kb on + strand at 4229.691 kb on - strand, within Pf1N1B4_3818 at 4229.816 kb on + strand, within Pf1N1B4_3818 at 4229.954 kb on + strand at 4229.954 kb on + strand, within Pf1N1B4_3818 at 4230.062 kb on + strand, within Pf1N1B4_3818 at 4230.062 kb on + strand, within Pf1N1B4_3818 at 4230.062 kb on + strand, within Pf1N1B4_3818 at 4230.063 kb on - strand, within Pf1N1B4_3818 at 4230.218 kb on + strand at 4230.218 kb on + strand at 4230.394 kb on + strand, within Pf1N1B4_3819 at 4230.394 kb on + strand, within Pf1N1B4_3819 at 4230.395 kb on - strand, within Pf1N1B4_3819 at 4230.648 kb on + strand, within Pf1N1B4_3819 at 4230.651 kb on + strand at 4230.652 kb on - strand, within Pf1N1B4_3819 at 4230.759 kb on - strand, within Pf1N1B4_3819 at 4230.853 kb on + strand at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.853 kb on + strand at 4230.853 kb on + strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.854 kb on - strand, within Pf1N1B4_3819 at 4230.925 kb on + strand, within Pf1N1B4_3819 at 4230.925 kb on + strand, within Pf1N1B4_3819 at 4230.926 kb on - strand, within Pf1N1B4_3819 at 4230.926 kb on - strand, within Pf1N1B4_3819 at 4230.926 kb on - strand at 4231.019 kb on - strand, within Pf1N1B4_3819 at 4231.019 kb on - strand, within Pf1N1B4_3819 at 4231.151 kb on - strand at 4231.201 kb on - strand at 4231.201 kb on - strand at 4231.229 kb on + strand at 4231.229 kb on + strand at 4231.229 kb on + strand at 4231.353 kb on + strand at 4231.353 kb on + strand at 4231.436 kb on + strand at 4231.436 kb on + strand at 4231.437 kb on - strand at 4231.541 kb on + strand, within Pf1N1B4_3820 at 4231.542 kb on - strand, within Pf1N1B4_3820 at 4231.743 kb on - strand, within Pf1N1B4_3820 at 4231.955 kb on + strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4231.956 kb on - strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.126 kb on + strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.127 kb on - strand at 4232.127 kb on - strand, within Pf1N1B4_3820 at 4232.128 kb on + strand, within Pf1N1B4_3820 at 4232.129 kb on - strand, within Pf1N1B4_3820
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,229,311 + +0.6 4,229,311 + +1.1 4,229,311 + +1.2 4,229,311 + +1.3 4,229,317 + +1.8 4,229,318 - -1.6 4,229,371 + -0.2 4,229,371 + -1.2 4,229,371 + +0.6 4,229,371 + +0.9 4,229,371 + +0.8 4,229,371 + -0.5 4,229,385 + +0.5 4,229,410 + -0.4 4,229,412 + -0.8 4,229,412 + -0.4 4,229,412 + +1.8 4,229,499 + +1.6 4,229,542 + -0.5 4,229,691 - Pf1N1B4_3818 0.20 +1.4 4,229,816 + Pf1N1B4_3818 0.38 -0.9 4,229,954 + -0.2 4,229,954 + Pf1N1B4_3818 0.57 +1.2 4,230,062 + Pf1N1B4_3818 0.72 -0.5 4,230,062 + Pf1N1B4_3818 0.72 -0.3 4,230,062 + Pf1N1B4_3818 0.72 -0.8 4,230,063 - Pf1N1B4_3818 0.72 +0.8 4,230,218 + -0.4 4,230,218 + +1.0 4,230,394 + Pf1N1B4_3819 0.15 -2.0 4,230,394 + Pf1N1B4_3819 0.15 -2.0 4,230,395 - Pf1N1B4_3819 0.15 -1.7 4,230,648 + Pf1N1B4_3819 0.43 +0.6 4,230,651 + -0.2 4,230,652 - Pf1N1B4_3819 0.44 -1.0 4,230,759 - Pf1N1B4_3819 0.56 -0.8 4,230,853 + -1.2 4,230,853 + Pf1N1B4_3819 0.66 -0.9 4,230,853 + Pf1N1B4_3819 0.66 -1.0 4,230,853 + +1.4 4,230,853 + Pf1N1B4_3819 0.66 -1.8 4,230,854 - Pf1N1B4_3819 0.66 +0.7 4,230,854 - Pf1N1B4_3819 0.66 -1.5 4,230,854 - Pf1N1B4_3819 0.66 +1.4 4,230,854 - Pf1N1B4_3819 0.66 -0.8 4,230,854 - Pf1N1B4_3819 0.66 -0.8 4,230,925 + Pf1N1B4_3819 0.74 -1.2 4,230,925 + Pf1N1B4_3819 0.74 +1.1 4,230,926 - Pf1N1B4_3819 0.75 -1.3 4,230,926 - Pf1N1B4_3819 0.75 -1.5 4,230,926 - -0.2 4,231,019 - Pf1N1B4_3819 0.85 +0.3 4,231,019 - Pf1N1B4_3819 0.85 -0.2 4,231,151 - -1.0 4,231,201 - -0.2 4,231,201 - +0.1 4,231,229 + +0.8 4,231,229 + +0.5 4,231,229 + +0.8 4,231,353 + -1.4 4,231,353 + +0.4 4,231,436 + -2.0 4,231,436 + -0.2 4,231,437 - -0.8 4,231,541 + Pf1N1B4_3820 0.18 -0.6 4,231,542 - Pf1N1B4_3820 0.18 -0.1 4,231,743 - Pf1N1B4_3820 0.36 -0.9 4,231,955 + Pf1N1B4_3820 0.55 +2.1 4,231,956 - Pf1N1B4_3820 0.55 +0.8 4,231,956 - Pf1N1B4_3820 0.55 +0.8 4,231,956 - Pf1N1B4_3820 0.55 +0.1 4,232,126 + Pf1N1B4_3820 0.70 +0.1 4,232,126 + Pf1N1B4_3820 0.70 -0.0 4,232,126 + Pf1N1B4_3820 0.70 -1.5 4,232,126 + Pf1N1B4_3820 0.70 +1.1 4,232,126 + Pf1N1B4_3820 0.70 +0.8 4,232,126 + Pf1N1B4_3820 0.70 -0.2 4,232,126 + -0.2 4,232,126 + Pf1N1B4_3820 0.70 +0.5 4,232,126 + Pf1N1B4_3820 0.70 +1.2 4,232,127 - Pf1N1B4_3820 0.70 -0.0 4,232,127 - Pf1N1B4_3820 0.70 +1.4 4,232,127 - Pf1N1B4_3820 0.70 -0.4 4,232,127 - Pf1N1B4_3820 0.70 -0.2 4,232,127 - +0.8 4,232,127 - Pf1N1B4_3820 0.70 +2.0 4,232,128 + Pf1N1B4_3820 0.70 +0.8 4,232,129 - Pf1N1B4_3820 0.70 +1.4
Or see this region's nucleotide sequence