Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3787 and Pf1N1B4_3788 are separated by 306 nucleotides Pf1N1B4_3788 and Pf1N1B4_3789 are separated by 215 nucleotides Pf1N1B4_3789 and Pf1N1B4_3790 are separated by 459 nucleotides
Pf1N1B4_3787 - FIG00960829: hypothetical protein, at 4,196,261 to 4,196,452
_3787
Pf1N1B4_3788 - FIG00957412: hypothetical protein, at 4,196,759 to 4,196,992
_3788
Pf1N1B4_3789 - Superfamily II DNA/RNA helicases, SNF2 family, at 4,197,208 to 4,197,549
_3789
Pf1N1B4_3790 - Protein containing domains DUF404, DUF407, at 4,198,009 to 4,199,418
_3790
Position (kb)
4197
4198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.229 kb on - strand at 4196.238 kb on + strand at 4196.335 kb on + strand, within Pf1N1B4_3787 at 4196.335 kb on + strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.377 kb on - strand, within Pf1N1B4_3787 at 4196.377 kb on - strand, within Pf1N1B4_3787 at 4196.571 kb on + strand at 4196.571 kb on + strand at 4196.572 kb on - strand at 4196.705 kb on + strand at 4196.718 kb on + strand at 4196.718 kb on + strand at 4196.718 kb on + strand at 4196.719 kb on - strand at 4196.719 kb on - strand at 4196.719 kb on - strand at 4196.719 kb on - strand at 4196.724 kb on + strand at 4196.724 kb on + strand at 4196.724 kb on + strand at 4196.725 kb on - strand at 4196.725 kb on - strand at 4196.725 kb on - strand at 4196.725 kb on - strand at 4196.725 kb on - strand at 4196.977 kb on + strand at 4196.977 kb on + strand at 4196.978 kb on - strand at 4196.978 kb on - strand at 4196.994 kb on - strand at 4196.994 kb on - strand at 4197.036 kb on + strand at 4197.037 kb on - strand at 4197.037 kb on - strand at 4197.099 kb on + strand at 4197.099 kb on + strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.100 kb on - strand at 4197.182 kb on - strand at 4197.182 kb on - strand at 4197.304 kb on + strand, within Pf1N1B4_3789 at 4197.304 kb on + strand, within Pf1N1B4_3789 at 4197.305 kb on - strand, within Pf1N1B4_3789 at 4197.430 kb on + strand, within Pf1N1B4_3789 at 4197.430 kb on + strand, within Pf1N1B4_3789 at 4197.431 kb on - strand, within Pf1N1B4_3789 at 4197.439 kb on + strand, within Pf1N1B4_3789 at 4197.439 kb on + strand, within Pf1N1B4_3789 at 4197.530 kb on + strand at 4197.553 kb on + strand at 4198.021 kb on + strand at 4198.021 kb on + strand at 4198.022 kb on - strand at 4198.022 kb on - strand at 4198.036 kb on + strand at 4198.036 kb on + strand at 4198.036 kb on + strand at 4198.036 kb on + strand at 4198.036 kb on + strand at 4198.036 kb on + strand at 4198.037 kb on - strand at 4198.037 kb on - strand at 4198.037 kb on - strand at 4198.037 kb on - strand at 4198.037 kb on - strand at 4198.142 kb on + strand at 4198.142 kb on + strand at 4198.174 kb on + strand, within Pf1N1B4_3790 at 4198.174 kb on + strand, within Pf1N1B4_3790 at 4198.174 kb on + strand, within Pf1N1B4_3790 at 4198.174 kb on + strand, within Pf1N1B4_3790 at 4198.230 kb on + strand, within Pf1N1B4_3790 at 4198.420 kb on + strand, within Pf1N1B4_3790 at 4198.420 kb on + strand, within Pf1N1B4_3790 at 4198.420 kb on + strand, within Pf1N1B4_3790 at 4198.420 kb on + strand at 4198.420 kb on + strand, within Pf1N1B4_3790 at 4198.448 kb on + strand, within Pf1N1B4_3790 at 4198.468 kb on + strand, within Pf1N1B4_3790 at 4198.468 kb on + strand, within Pf1N1B4_3790 at 4198.468 kb on + strand, within Pf1N1B4_3790 at 4198.468 kb on + strand, within Pf1N1B4_3790 at 4198.468 kb on + strand, within Pf1N1B4_3790 at 4198.468 kb on + strand at 4198.469 kb on - strand, within Pf1N1B4_3790 at 4198.471 kb on + strand, within Pf1N1B4_3790 at 4198.471 kb on + strand, within Pf1N1B4_3790 at 4198.472 kb on - strand, within Pf1N1B4_3790 at 4198.472 kb on - strand, within Pf1N1B4_3790 at 4198.472 kb on - strand, within Pf1N1B4_3790 at 4198.472 kb on - strand, within Pf1N1B4_3790 at 4198.513 kb on + strand at 4198.513 kb on + strand, within Pf1N1B4_3790 at 4198.513 kb on + strand, within Pf1N1B4_3790 at 4198.513 kb on + strand, within Pf1N1B4_3790 at 4198.513 kb on + strand, within Pf1N1B4_3790 at 4198.514 kb on - strand, within Pf1N1B4_3790 at 4198.514 kb on - strand, within Pf1N1B4_3790
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 4,196,228 + -2.6 4,196,228 + -0.7 4,196,228 + -0.4 4,196,228 + +0.1 4,196,228 + -2.3 4,196,228 + -0.7 4,196,228 + -1.2 4,196,229 - -0.5 4,196,238 + +1.4 4,196,335 + Pf1N1B4_3787 0.39 +1.5 4,196,335 + Pf1N1B4_3787 0.39 +0.3 4,196,336 - Pf1N1B4_3787 0.39 +1.8 4,196,336 - Pf1N1B4_3787 0.39 -0.4 4,196,336 - Pf1N1B4_3787 0.39 +0.9 4,196,336 - Pf1N1B4_3787 0.39 +0.8 4,196,377 - Pf1N1B4_3787 0.60 -1.3 4,196,377 - Pf1N1B4_3787 0.60 -0.2 4,196,571 + -0.2 4,196,571 + -0.3 4,196,572 - +0.1 4,196,705 + +0.3 4,196,718 + -0.2 4,196,718 + -0.6 4,196,718 + +0.4 4,196,719 - -0.4 4,196,719 - -0.4 4,196,719 - -0.7 4,196,719 - -0.7 4,196,724 + +0.3 4,196,724 + +0.8 4,196,724 + -1.7 4,196,725 - -0.2 4,196,725 - -2.0 4,196,725 - -0.2 4,196,725 - -2.3 4,196,725 - -1.0 4,196,977 + -0.1 4,196,977 + +1.8 4,196,978 - +0.8 4,196,978 - -2.2 4,196,994 - -1.3 4,196,994 - +0.5 4,197,036 + +1.4 4,197,037 - -0.2 4,197,037 - -0.5 4,197,099 + -1.7 4,197,099 + +0.1 4,197,100 - +0.4 4,197,100 - -0.2 4,197,100 - -1.2 4,197,100 - +0.5 4,197,100 - -0.2 4,197,100 - +0.4 4,197,182 - +0.6 4,197,182 - -0.7 4,197,304 + Pf1N1B4_3789 0.28 -2.0 4,197,304 + Pf1N1B4_3789 0.28 -1.7 4,197,305 - Pf1N1B4_3789 0.28 +0.8 4,197,430 + Pf1N1B4_3789 0.65 -1.0 4,197,430 + Pf1N1B4_3789 0.65 -0.5 4,197,431 - Pf1N1B4_3789 0.65 +0.5 4,197,439 + Pf1N1B4_3789 0.68 -1.3 4,197,439 + Pf1N1B4_3789 0.68 +0.4 4,197,530 + +0.8 4,197,553 + +1.4 4,198,021 + +1.2 4,198,021 + +0.2 4,198,022 - +1.0 4,198,022 - +0.3 4,198,036 + -2.7 4,198,036 + -2.7 4,198,036 + +0.1 4,198,036 + -1.2 4,198,036 + -0.0 4,198,036 + -1.7 4,198,037 - +1.8 4,198,037 - -1.2 4,198,037 - -0.6 4,198,037 - -0.4 4,198,037 - +0.2 4,198,142 + -0.8 4,198,142 + +0.1 4,198,174 + Pf1N1B4_3790 0.12 -0.5 4,198,174 + Pf1N1B4_3790 0.12 -1.0 4,198,174 + Pf1N1B4_3790 0.12 -2.8 4,198,174 + Pf1N1B4_3790 0.12 -1.1 4,198,230 + Pf1N1B4_3790 0.16 +1.8 4,198,420 + Pf1N1B4_3790 0.29 +0.2 4,198,420 + Pf1N1B4_3790 0.29 -0.3 4,198,420 + Pf1N1B4_3790 0.29 -0.2 4,198,420 + +0.8 4,198,420 + Pf1N1B4_3790 0.29 -1.2 4,198,448 + Pf1N1B4_3790 0.31 +0.2 4,198,468 + Pf1N1B4_3790 0.33 +1.2 4,198,468 + Pf1N1B4_3790 0.33 +0.8 4,198,468 + Pf1N1B4_3790 0.33 +0.7 4,198,468 + Pf1N1B4_3790 0.33 -1.6 4,198,468 + Pf1N1B4_3790 0.33 +0.0 4,198,468 + +2.4 4,198,469 - Pf1N1B4_3790 0.33 +1.5 4,198,471 + Pf1N1B4_3790 0.33 -1.2 4,198,471 + Pf1N1B4_3790 0.33 +0.8 4,198,472 - Pf1N1B4_3790 0.33 +0.8 4,198,472 - Pf1N1B4_3790 0.33 +0.2 4,198,472 - Pf1N1B4_3790 0.33 +0.3 4,198,472 - Pf1N1B4_3790 0.33 -0.5 4,198,513 + +0.2 4,198,513 + Pf1N1B4_3790 0.36 -0.8 4,198,513 + Pf1N1B4_3790 0.36 +0.5 4,198,513 + Pf1N1B4_3790 0.36 +1.8 4,198,513 + Pf1N1B4_3790 0.36 +0.2 4,198,514 - Pf1N1B4_3790 0.36 -1.5 4,198,514 - Pf1N1B4_3790 0.36 +0.4
Or see this region's nucleotide sequence