Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3750

Experiment: Spectinomycin 0.05 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.05 mg/ml
4,160,613	+				+0.4
4,160,613	+				-1.5
4,160,614	-				-0.4
4,160,614	-				+0.6
4,160,614	-				-0.1
4,160,624	+				-1.1
4,160,625	-				+0.0
4,160,697	+				-1.0
4,160,863	+		Pf1N1B4_3749	0.22	+1.3
4,160,940	+		Pf1N1B4_3749	0.32	+0.1
4,160,940	+		Pf1N1B4_3749	0.32	-0.1
4,161,129	+		Pf1N1B4_3749	0.59	-1.1
4,161,129	+		Pf1N1B4_3749	0.59	+0.6
4,161,129	+		Pf1N1B4_3749	0.59	+1.9
4,161,129	+		Pf1N1B4_3749	0.59	-0.1
4,161,129	+		Pf1N1B4_3749	0.59	-0.1
4,161,129	+		Pf1N1B4_3749	0.59	+0.1
4,161,129	+		Pf1N1B4_3749	0.59	+0.7
4,161,129	+		Pf1N1B4_3749	0.59	-0.4
4,161,130	-		Pf1N1B4_3749	0.59	-1.1
4,161,130	-		Pf1N1B4_3749	0.59	+2.5
4,161,130	-		Pf1N1B4_3749	0.59	+0.6
4,161,130	-		Pf1N1B4_3749	0.59	+0.1
4,161,130	-		Pf1N1B4_3749	0.59	-1.5
4,161,141	+		Pf1N1B4_3749	0.61	-0.6
4,161,141	+		Pf1N1B4_3749	0.61	+0.4
4,161,207	+		Pf1N1B4_3749	0.70	+0.7
4,161,207	+		Pf1N1B4_3749	0.70	-0.4
4,161,208	-		Pf1N1B4_3749	0.70	+0.6
4,161,208	-		Pf1N1B4_3749	0.70	+0.3
4,161,208	-		Pf1N1B4_3749	0.70	+0.1
4,161,208	-				+1.3
4,161,339	+		Pf1N1B4_3749	0.88	+1.2
4,161,528	+				-0.4
4,161,528	+				+2.9
4,161,528	+				-0.1
4,161,528	+				-0.7
4,161,528	+				+2.4
4,161,528	+				-0.5
4,161,528	+				+1.0
4,161,528	+				+0.1
4,161,528	+				-1.3
4,161,529	-				-0.1
4,161,529	-				-0.1
4,161,529	-				-1.7
4,161,529	-				+2.4
4,161,573	-				-1.7
4,161,585	-				-0.6
4,161,585	-				-0.7
4,161,656	-				-3.0
4,162,031	+		Pf1N1B4_3750	0.21	+0.7
4,162,967	+		Pf1N1B4_3750	0.66	+0.7
4,162,967	+		Pf1N1B4_3750	0.66	-0.6
4,162,968	-		Pf1N1B4_3750	0.66	-0.1
4,163,037	+				+1.3
4,163,103	-		Pf1N1B4_3750	0.73	+0.0
4,163,111	+		Pf1N1B4_3750	0.73	+0.6
4,163,111	+		Pf1N1B4_3750	0.73	+3.4
4,163,111	+		Pf1N1B4_3750	0.73	-0.7
4,163,112	-		Pf1N1B4_3750	0.73	+1.9
4,163,515	+				+0.9
4,163,516	-				-0.3
4,163,751	+				+1.0
4,163,752	-				+0.9
4,163,752	-				-0.6
4,163,792	+				+0.9
4,163,792	+				-0.5
4,163,792	+				+0.3
4,163,819	+				+1.3
4,163,819	+		Pf1N1B4_3751	0.16	-2.5
4,163,819	+		Pf1N1B4_3751	0.16	-1.0
4,163,820	-		Pf1N1B4_3751	0.16	+0.0
4,163,820	-				-1.1
4,163,991	+				+0.5
4,164,002	+				+0.4
4,164,002	+				-1.5
4,164,104	+				-0.5
4,164,104	+				-1.3
4,164,110	+				-0.5
4,164,111	-				+2.7
4,164,140	+				+1.9
4,164,175	+				-0.8
4,164,196	+				+1.4
4,164,196	+				+0.4
4,164,196	+				+0.3
4,164,196	+				-0.1
4,164,197	-				-3.1
4,164,197	-				+0.0
4,164,197	-				-0.3
4,164,197	-				-0.5
4,164,197	-				-0.4
4,164,335	+		Pf1N1B4_3752	0.12	-0.0
4,164,335	+		Pf1N1B4_3752	0.12	+0.4
4,164,335	+		Pf1N1B4_3752	0.12	-0.2
4,164,335	+		Pf1N1B4_3752	0.12	-0.1
4,164,336	-		Pf1N1B4_3752	0.13	+0.7
4,164,336	-		Pf1N1B4_3752	0.13	+0.3
4,164,336	-		Pf1N1B4_3752	0.13	-0.1
4,164,336	-		Pf1N1B4_3752	0.13	-0.1
4,164,381	+		Pf1N1B4_3752	0.16	+0.6
4,164,381	+		Pf1N1B4_3752	0.16	-1.0
4,164,381	+		Pf1N1B4_3752	0.16	-0.4
4,164,382	-		Pf1N1B4_3752	0.16	-0.0
4,164,517	+		Pf1N1B4_3752	0.27	+0.9
4,164,517	+		Pf1N1B4_3752	0.27	-0.5
4,164,517	+		Pf1N1B4_3752	0.27	-0.5
4,164,517	+		Pf1N1B4_3752	0.27	+1.4
4,164,517	+		Pf1N1B4_3752	0.27	-2.3
4,164,517	+		Pf1N1B4_3752	0.27	-1.5
4,164,518	-				+0.3
4,164,518	-		Pf1N1B4_3752	0.27	-1.0
4,164,518	-		Pf1N1B4_3752	0.27	-0.6
4,164,518	-		Pf1N1B4_3752	0.27	-0.6
4,164,518	-		Pf1N1B4_3752	0.27	-0.5
4,164,518	-		Pf1N1B4_3752	0.27	+0.5
4,164,518	-		Pf1N1B4_3752	0.27	+0.9
4,164,518	-		Pf1N1B4_3752	0.27	-0.6
4,164,579	+		Pf1N1B4_3752	0.31	+0.4