Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3500 and Pf1N1B4_3501 are separated by 199 nucleotides Pf1N1B4_3501 and Pf1N1B4_3502 are separated by 23 nucleotides Pf1N1B4_3502 and Pf1N1B4_3503 overlap by 21 nucleotides Pf1N1B4_3503 and Pf1N1B4_3504 are separated by 1 nucleotides
Pf1N1B4_3500 - D-lactate dehydrogenase (EC 1.1.1.28), at 3,904,118 to 3,905,107
_3500
Pf1N1B4_3501 - BarA sensory histidine kinase (= VarS = GacS), at 3,905,307 to 3,908,057
_3501
Pf1N1B4_3502 - hypothetical protein, at 3,908,081 to 3,908,347
_3502
Pf1N1B4_3503 - Two-component response regulator PfeR, enterobactin, at 3,908,327 to 3,908,995
_3503
Pf1N1B4_3504 - Two-component sensor histidine kinase PfeS, enterobactin, at 3,908,997 to 3,910,412
_3504
Position (kb)
3905
3906
3907
3908
3909 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3904.330 kb on - strand, within Pf1N1B4_3500 at 3904.330 kb on - strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.421 kb on + strand, within Pf1N1B4_3500 at 3904.422 kb on - strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.737 kb on + strand, within Pf1N1B4_3500 at 3904.738 kb on - strand at 3904.809 kb on + strand, within Pf1N1B4_3500 at 3904.809 kb on + strand, within Pf1N1B4_3500 at 3904.810 kb on - strand, within Pf1N1B4_3500 at 3904.929 kb on - strand, within Pf1N1B4_3500 at 3905.076 kb on + strand at 3905.077 kb on - strand at 3905.125 kb on + strand at 3905.125 kb on + strand at 3905.126 kb on - strand at 3905.126 kb on - strand at 3905.132 kb on - strand at 3905.219 kb on - strand at 3905.273 kb on + strand at 3905.295 kb on + strand at 3905.607 kb on + strand, within Pf1N1B4_3501 at 3905.607 kb on + strand at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.608 kb on - strand, within Pf1N1B4_3501 at 3905.752 kb on + strand, within Pf1N1B4_3501 at 3905.752 kb on + strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand, within Pf1N1B4_3501 at 3905.753 kb on - strand at 3906.266 kb on + strand, within Pf1N1B4_3501 at 3906.266 kb on + strand at 3906.266 kb on + strand, within Pf1N1B4_3501 at 3906.266 kb on + strand, within Pf1N1B4_3501 at 3906.267 kb on - strand at 3906.267 kb on - strand, within Pf1N1B4_3501 at 3906.267 kb on - strand, within Pf1N1B4_3501 at 3906.766 kb on + strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand, within Pf1N1B4_3501 at 3906.767 kb on - strand at 3906.891 kb on - strand, within Pf1N1B4_3501 at 3907.093 kb on + strand, within Pf1N1B4_3501 at 3907.093 kb on + strand, within Pf1N1B4_3501 at 3907.094 kb on - strand, within Pf1N1B4_3501 at 3907.973 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.037 kb on - strand at 3908.082 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.339 kb on + strand at 3908.340 kb on - strand at 3908.585 kb on + strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.786 kb on - strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand, within Pf1N1B4_3503 at 3908.811 kb on + strand at 3908.812 kb on - strand at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.812 kb on - strand, within Pf1N1B4_3503 at 3908.841 kb on + strand, within Pf1N1B4_3503 at 3908.841 kb on + strand, within Pf1N1B4_3503 at 3908.945 kb on - strand at 3908.963 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.967 kb on + strand at 3908.968 kb on - strand at 3908.968 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 3,904,330 - Pf1N1B4_3500 0.21 +0.9 3,904,330 - Pf1N1B4_3500 0.21 +0.9 3,904,421 + Pf1N1B4_3500 0.31 -2.6 3,904,421 + Pf1N1B4_3500 0.31 -0.6 3,904,421 + Pf1N1B4_3500 0.31 +0.9 3,904,421 + Pf1N1B4_3500 0.31 +0.6 3,904,422 - Pf1N1B4_3500 0.31 +1.2 3,904,737 + Pf1N1B4_3500 0.63 +0.1 3,904,737 + Pf1N1B4_3500 0.63 -1.0 3,904,737 + Pf1N1B4_3500 0.63 -0.1 3,904,737 + Pf1N1B4_3500 0.63 -0.3 3,904,738 - -0.1 3,904,809 + Pf1N1B4_3500 0.70 +1.1 3,904,809 + Pf1N1B4_3500 0.70 +0.7 3,904,810 - Pf1N1B4_3500 0.70 -0.0 3,904,929 - Pf1N1B4_3500 0.82 -1.4 3,905,076 + +1.2 3,905,077 - +0.9 3,905,125 + +0.6 3,905,125 + -2.4 3,905,126 - -0.3 3,905,126 - -0.1 3,905,132 - -1.3 3,905,219 - -0.1 3,905,273 + +1.5 3,905,295 + +0.6 3,905,607 + Pf1N1B4_3501 0.11 -2.3 3,905,607 + +0.9 3,905,608 - Pf1N1B4_3501 0.11 -0.2 3,905,608 - Pf1N1B4_3501 0.11 -0.7 3,905,608 - Pf1N1B4_3501 0.11 -1.1 3,905,752 + Pf1N1B4_3501 0.16 +0.9 3,905,752 + Pf1N1B4_3501 0.16 -2.1 3,905,753 - Pf1N1B4_3501 0.16 -0.1 3,905,753 - Pf1N1B4_3501 0.16 -0.6 3,905,753 - Pf1N1B4_3501 0.16 +0.2 3,905,753 - +0.9 3,906,266 + Pf1N1B4_3501 0.35 -1.2 3,906,266 + +1.2 3,906,266 + Pf1N1B4_3501 0.35 -0.1 3,906,266 + Pf1N1B4_3501 0.35 -1.8 3,906,267 - -0.9 3,906,267 - Pf1N1B4_3501 0.35 -0.1 3,906,267 - Pf1N1B4_3501 0.35 -2.8 3,906,766 + Pf1N1B4_3501 0.53 -1.4 3,906,767 - Pf1N1B4_3501 0.53 -1.9 3,906,767 - Pf1N1B4_3501 0.53 -2.1 3,906,767 - Pf1N1B4_3501 0.53 +0.2 3,906,767 - Pf1N1B4_3501 0.53 -0.4 3,906,767 - Pf1N1B4_3501 0.53 -1.7 3,906,767 - Pf1N1B4_3501 0.53 +0.3 3,906,767 - Pf1N1B4_3501 0.53 -1.7 3,906,767 - -0.7 3,906,891 - Pf1N1B4_3501 0.58 -0.8 3,907,093 + Pf1N1B4_3501 0.65 -1.9 3,907,093 + Pf1N1B4_3501 0.65 +0.1 3,907,094 - Pf1N1B4_3501 0.65 +0.2 3,907,973 - -0.9 3,908,037 - -0.1 3,908,037 - -0.3 3,908,037 - -1.1 3,908,037 - -0.1 3,908,082 + -0.3 3,908,339 + -1.2 3,908,339 + -0.7 3,908,339 + +2.2 3,908,339 + -0.5 3,908,340 - -0.4 3,908,585 + Pf1N1B4_3503 0.39 +0.9 3,908,786 - Pf1N1B4_3503 0.69 -1.7 3,908,786 - Pf1N1B4_3503 0.69 +0.6 3,908,786 - Pf1N1B4_3503 0.69 -1.2 3,908,786 - Pf1N1B4_3503 0.69 -0.3 3,908,811 + Pf1N1B4_3503 0.72 -0.0 3,908,811 + Pf1N1B4_3503 0.72 +0.3 3,908,811 + Pf1N1B4_3503 0.72 +0.4 3,908,811 + +0.3 3,908,812 - +0.3 3,908,812 - Pf1N1B4_3503 0.72 -1.1 3,908,812 - Pf1N1B4_3503 0.72 +0.5 3,908,812 - Pf1N1B4_3503 0.72 +0.7 3,908,841 + Pf1N1B4_3503 0.77 -0.7 3,908,841 + Pf1N1B4_3503 0.77 -1.1 3,908,945 - -0.4 3,908,963 + -1.2 3,908,967 + -0.5 3,908,967 + -0.4 3,908,967 + -0.4 3,908,967 + +1.2 3,908,968 - +0.1 3,908,968 - -1.4
Or see this region's nucleotide sequence