Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5223 and Pf1N1B4_3453 are separated by 22 nucleotides Pf1N1B4_3453 and Pf1N1B4_3454 are separated by 660 nucleotides Pf1N1B4_3454 and Pf1N1B4_3455 are separated by 198 nucleotides Pf1N1B4_3455 and Pf1N1B4_3456 are separated by 411 nucleotides
Pf1N1B4_5223 - tRNA-Arg-ACG, at 3,850,562 to 3,850,635
_5223
Pf1N1B4_3453 - hypothetical protein, at 3,850,658 to 3,850,834
_3453
Pf1N1B4_3454 - Mg/Co/Ni transporter MgtE / CBS domain, at 3,851,495 to 3,852,937
_3454
Pf1N1B4_3455 - Alginate biosynthesis transcriptional activator, at 3,853,136 to 3,853,462
_3455
Pf1N1B4_3456 - ATP-binding protein PhnN; Guanylate kinase (EC 2.7.4.8), at 3,853,874 to 3,854,422
_3456
Position (kb)
3851
3852
3853 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3850.547 kb on + strand at 3850.549 kb on + strand at 3850.549 kb on + strand at 3850.802 kb on - strand, within Pf1N1B4_3453 at 3851.018 kb on + strand at 3851.019 kb on - strand at 3851.019 kb on - strand at 3851.019 kb on - strand at 3851.019 kb on - strand at 3851.019 kb on - strand at 3851.050 kb on + strand at 3851.051 kb on - strand at 3851.051 kb on - strand at 3851.154 kb on + strand at 3851.207 kb on - strand at 3851.207 kb on - strand at 3851.214 kb on - strand at 3851.214 kb on - strand at 3851.214 kb on - strand at 3851.214 kb on - strand at 3851.350 kb on + strand at 3851.388 kb on + strand at 3851.410 kb on - strand at 3851.431 kb on + strand at 3851.432 kb on - strand at 3851.441 kb on + strand at 3851.442 kb on - strand at 3851.494 kb on + strand at 3851.495 kb on - strand at 3851.705 kb on + strand, within Pf1N1B4_3454 at 3851.705 kb on + strand, within Pf1N1B4_3454 at 3851.705 kb on + strand, within Pf1N1B4_3454 at 3851.705 kb on + strand, within Pf1N1B4_3454 at 3851.706 kb on - strand, within Pf1N1B4_3454 at 3851.706 kb on - strand, within Pf1N1B4_3454 at 3851.793 kb on + strand, within Pf1N1B4_3454 at 3851.793 kb on + strand, within Pf1N1B4_3454 at 3851.794 kb on - strand, within Pf1N1B4_3454 at 3851.978 kb on + strand, within Pf1N1B4_3454 at 3851.978 kb on + strand, within Pf1N1B4_3454 at 3852.025 kb on + strand, within Pf1N1B4_3454 at 3852.239 kb on + strand, within Pf1N1B4_3454 at 3852.240 kb on - strand, within Pf1N1B4_3454 at 3852.240 kb on - strand, within Pf1N1B4_3454 at 3852.266 kb on + strand, within Pf1N1B4_3454 at 3852.640 kb on + strand, within Pf1N1B4_3454 at 3852.640 kb on + strand, within Pf1N1B4_3454 at 3852.640 kb on + strand, within Pf1N1B4_3454 at 3852.640 kb on + strand, within Pf1N1B4_3454 at 3852.640 kb on + strand, within Pf1N1B4_3454 at 3852.640 kb on + strand, within Pf1N1B4_3454 at 3852.722 kb on + strand, within Pf1N1B4_3454 at 3852.731 kb on + strand, within Pf1N1B4_3454 at 3852.778 kb on + strand, within Pf1N1B4_3454 at 3852.778 kb on + strand, within Pf1N1B4_3454 at 3852.778 kb on + strand at 3852.778 kb on + strand, within Pf1N1B4_3454 at 3853.013 kb on + strand at 3853.110 kb on - strand at 3853.110 kb on - strand at 3853.110 kb on - strand at 3853.110 kb on - strand at 3853.307 kb on - strand, within Pf1N1B4_3455 at 3853.407 kb on - strand, within Pf1N1B4_3455 at 3853.438 kb on - strand at 3853.438 kb on - strand at 3853.463 kb on - strand at 3853.465 kb on - strand at 3853.520 kb on + strand at 3853.559 kb on + strand at 3853.617 kb on + strand at 3853.617 kb on + strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.618 kb on - strand at 3853.637 kb on + strand at 3853.650 kb on + strand at 3853.650 kb on + strand at 3853.650 kb on + strand at 3853.650 kb on + strand at 3853.680 kb on + strand at 3853.680 kb on + strand at 3853.757 kb on + strand at 3853.757 kb on + strand at 3853.757 kb on + strand at 3853.757 kb on + strand at 3853.758 kb on - strand at 3853.781 kb on + strand at 3853.786 kb on + strand at 3853.786 kb on + strand at 3853.787 kb on - strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.788 kb on + strand at 3853.789 kb on - strand at 3853.789 kb on - strand at 3853.789 kb on - strand at 3853.789 kb on - strand at 3853.814 kb on + strand at 3853.814 kb on + strand at 3853.814 kb on + strand at 3853.815 kb on - strand at 3853.815 kb on - strand at 3853.818 kb on - strand at 3853.829 kb on - strand at 3853.863 kb on + strand at 3853.868 kb on + strand at 3853.868 kb on + strand at 3853.868 kb on + strand at 3853.868 kb on + strand at 3853.869 kb on - strand at 3853.873 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 3,850,547 + -1.4 3,850,549 + -1.9 3,850,549 + +0.2 3,850,802 - Pf1N1B4_3453 0.81 +0.7 3,851,018 + +1.8 3,851,019 - +0.4 3,851,019 - -1.1 3,851,019 - +0.5 3,851,019 - +0.4 3,851,019 - +2.3 3,851,050 + -1.1 3,851,051 - -0.4 3,851,051 - -0.1 3,851,154 + -0.6 3,851,207 - +0.9 3,851,207 - -0.1 3,851,214 - -0.6 3,851,214 - +1.7 3,851,214 - -1.6 3,851,214 - -1.1 3,851,350 + +0.6 3,851,388 + -1.4 3,851,410 - -0.6 3,851,431 + -0.2 3,851,432 - -1.1 3,851,441 + +0.9 3,851,442 - -0.6 3,851,494 + -1.8 3,851,495 - -0.1 3,851,705 + Pf1N1B4_3454 0.15 +0.1 3,851,705 + Pf1N1B4_3454 0.15 -0.2 3,851,705 + Pf1N1B4_3454 0.15 -2.6 3,851,705 + Pf1N1B4_3454 0.15 -0.5 3,851,706 - Pf1N1B4_3454 0.15 -1.1 3,851,706 - Pf1N1B4_3454 0.15 -0.1 3,851,793 + Pf1N1B4_3454 0.21 +0.9 3,851,793 + Pf1N1B4_3454 0.21 -1.1 3,851,794 - Pf1N1B4_3454 0.21 -1.1 3,851,978 + Pf1N1B4_3454 0.33 -1.4 3,851,978 + Pf1N1B4_3454 0.33 +1.9 3,852,025 + Pf1N1B4_3454 0.37 -2.5 3,852,239 + Pf1N1B4_3454 0.52 -0.9 3,852,240 - Pf1N1B4_3454 0.52 -0.6 3,852,240 - Pf1N1B4_3454 0.52 -1.4 3,852,266 + Pf1N1B4_3454 0.53 -0.6 3,852,640 + Pf1N1B4_3454 0.79 +0.4 3,852,640 + Pf1N1B4_3454 0.79 -0.4 3,852,640 + Pf1N1B4_3454 0.79 +0.9 3,852,640 + Pf1N1B4_3454 0.79 -2.1 3,852,640 + Pf1N1B4_3454 0.79 -1.6 3,852,640 + Pf1N1B4_3454 0.79 -1.4 3,852,722 + Pf1N1B4_3454 0.85 -2.2 3,852,731 + Pf1N1B4_3454 0.86 -2.1 3,852,778 + Pf1N1B4_3454 0.89 -2.1 3,852,778 + Pf1N1B4_3454 0.89 -0.6 3,852,778 + +0.9 3,852,778 + Pf1N1B4_3454 0.89 -0.1 3,853,013 + +1.7 3,853,110 - -1.0 3,853,110 - +0.3 3,853,110 - +0.7 3,853,110 - +0.9 3,853,307 - Pf1N1B4_3455 0.52 +0.4 3,853,407 - Pf1N1B4_3455 0.83 -2.5 3,853,438 - -1.6 3,853,438 - -4.5 3,853,463 - -2.1 3,853,465 - -2.1 3,853,520 + -0.1 3,853,559 + +0.9 3,853,617 + -0.5 3,853,617 + -0.3 3,853,618 - +0.2 3,853,618 - -0.4 3,853,618 - +0.4 3,853,618 - +1.4 3,853,618 - +0.3 3,853,618 - +0.7 3,853,637 + +0.9 3,853,650 + -0.8 3,853,650 + -0.1 3,853,650 + -0.6 3,853,650 + +0.6 3,853,680 + +0.0 3,853,680 + -0.5 3,853,757 + +1.1 3,853,757 + +0.4 3,853,757 + -0.3 3,853,757 + +1.3 3,853,758 - +0.9 3,853,781 + +0.7 3,853,786 + +0.9 3,853,786 + +0.4 3,853,787 - +0.9 3,853,788 + +0.3 3,853,788 + -1.4 3,853,788 + -1.9 3,853,788 + -1.4 3,853,788 + -1.9 3,853,788 + -1.2 3,853,788 + +0.4 3,853,789 - -1.1 3,853,789 - -0.2 3,853,789 - +1.5 3,853,789 - +0.8 3,853,814 + +0.4 3,853,814 + -0.4 3,853,814 + -0.4 3,853,815 - -1.9 3,853,815 - -1.4 3,853,818 - -0.1 3,853,829 - -1.6 3,853,863 + +1.4 3,853,868 + -0.3 3,853,868 + -0.4 3,853,868 + -0.1 3,853,868 + +0.8 3,853,869 - -0.3 3,853,873 - +0.4
Or see this region's nucleotide sequence