Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3245

Experiment: Spectinomycin 0.05 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3244 and Pf1N1B4_3245 are separated by 146 nucleotidesPf1N1B4_3245 and Pf1N1B4_3246 overlap by 1 nucleotides Pf1N1B4_3244 - NADH oxidase, at 3,630,193 to 3,632,148 _3244 Pf1N1B4_3245 - RND efflux system, outer membrane lipoprotein, NodT family, at 3,632,295 to 3,633,755 _3245 Pf1N1B4_3246 - RND efflux system, inner membrane transporter CmeB, at 3,633,755 to 3,636,916 _3246 Position (kb) 3632 3633 3634Strain fitness (log2 ratio) -3 -2 -1 0 1at 3631.390 kb on + strand, within Pf1N1B4_3244at 3631.423 kb on + strand, within Pf1N1B4_3244at 3631.424 kb on - strand, within Pf1N1B4_3244at 3631.424 kb on - strand, within Pf1N1B4_3244at 3631.424 kb on - strand, within Pf1N1B4_3244at 3631.501 kb on + strand, within Pf1N1B4_3244at 3631.501 kb on + strand, within Pf1N1B4_3244at 3631.501 kb on + strand, within Pf1N1B4_3244at 3631.502 kb on - strand, within Pf1N1B4_3244at 3631.657 kb on + strand, within Pf1N1B4_3244at 3631.658 kb on - strand, within Pf1N1B4_3244at 3631.658 kb on - strand, within Pf1N1B4_3244at 3631.660 kb on + strand, within Pf1N1B4_3244at 3631.660 kb on + strandat 3631.660 kb on + strand, within Pf1N1B4_3244at 3631.660 kb on + strand, within Pf1N1B4_3244at 3631.660 kb on + strand, within Pf1N1B4_3244at 3631.660 kb on + strand, within Pf1N1B4_3244at 3631.660 kb on + strand, within Pf1N1B4_3244at 3631.661 kb on - strandat 3631.661 kb on - strand, within Pf1N1B4_3244at 3631.661 kb on - strand, within Pf1N1B4_3244at 3631.759 kb on + strand, within Pf1N1B4_3244at 3631.759 kb on + strand, within Pf1N1B4_3244at 3631.760 kb on - strand, within Pf1N1B4_3244at 3631.760 kb on - strand, within Pf1N1B4_3244at 3631.838 kb on + strand, within Pf1N1B4_3244at 3631.839 kb on - strand, within Pf1N1B4_3244at 3631.839 kb on - strand, within Pf1N1B4_3244at 3631.923 kb on + strand, within Pf1N1B4_3244at 3631.923 kb on + strand, within Pf1N1B4_3244at 3631.923 kb on + strand, within Pf1N1B4_3244at 3631.924 kb on - strand, within Pf1N1B4_3244at 3631.955 kb on - strandat 3632.190 kb on + strandat 3632.190 kb on + strandat 3632.190 kb on + strandat 3632.190 kb on + strandat 3632.193 kb on + strandat 3632.193 kb on + strandat 3632.193 kb on + strandat 3632.193 kb on + strandat 3632.381 kb on - strandat 3632.522 kb on - strand, within Pf1N1B4_3245at 3633.143 kb on - strand, within Pf1N1B4_3245at 3633.215 kb on - strand, within Pf1N1B4_3245at 3633.281 kb on - strand, within Pf1N1B4_3245at 3633.281 kb on - strand, within Pf1N1B4_3245at 3633.338 kb on - strand, within Pf1N1B4_3245at 3633.770 kb on - strandat 3633.770 kb on - strandat 3633.770 kb on - strandat 3634.037 kb on - strandat 3634.132 kb on - strand, within Pf1N1B4_3246at 3634.318 kb on - strand, within Pf1N1B4_3246at 3634.442 kb on + strand, within Pf1N1B4_3246at 3634.594 kb on - strand, within Pf1N1B4_3246at 3634.594 kb on - strand, within Pf1N1B4_3246at 3634.635 kb on + strand, within Pf1N1B4_3246at 3634.636 kb on - strand, within Pf1N1B4_3246at 3634.640 kb on - strandat 3634.640 kb on - strand, within Pf1N1B4_3246

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Per-strain Table

Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml
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3,631,390 + Pf1N1B4_3244 0.61 -0.6
3,631,423 + Pf1N1B4_3244 0.63 +0.4
3,631,424 - Pf1N1B4_3244 0.63 +1.4
3,631,424 - Pf1N1B4_3244 0.63 -0.6
3,631,424 - Pf1N1B4_3244 0.63 +0.3
3,631,501 + Pf1N1B4_3244 0.67 +0.0
3,631,501 + Pf1N1B4_3244 0.67 -2.0
3,631,501 + Pf1N1B4_3244 0.67 -0.2
3,631,502 - Pf1N1B4_3244 0.67 -2.1
3,631,657 + Pf1N1B4_3244 0.75 -0.2
3,631,658 - Pf1N1B4_3244 0.75 -1.3
3,631,658 - Pf1N1B4_3244 0.75 +0.4
3,631,660 + Pf1N1B4_3244 0.75 +0.8
3,631,660 + -1.0
3,631,660 + Pf1N1B4_3244 0.75 -0.6
3,631,660 + Pf1N1B4_3244 0.75 +0.3
3,631,660 + Pf1N1B4_3244 0.75 -2.2
3,631,660 + Pf1N1B4_3244 0.75 -1.0
3,631,660 + Pf1N1B4_3244 0.75 +0.2
3,631,661 - +1.0
3,631,661 - Pf1N1B4_3244 0.75 -0.6
3,631,661 - Pf1N1B4_3244 0.75 +0.3
3,631,759 + Pf1N1B4_3244 0.80 -1.3
3,631,759 + Pf1N1B4_3244 0.80 -2.0
3,631,760 - Pf1N1B4_3244 0.80 +0.6
3,631,760 - Pf1N1B4_3244 0.80 +0.4
3,631,838 + Pf1N1B4_3244 0.84 +1.0
3,631,839 - Pf1N1B4_3244 0.84 -1.8
3,631,839 - Pf1N1B4_3244 0.84 -0.1
3,631,923 + Pf1N1B4_3244 0.88 -0.3
3,631,923 + Pf1N1B4_3244 0.88 +1.4
3,631,923 + Pf1N1B4_3244 0.88 -0.3
3,631,924 - Pf1N1B4_3244 0.88 -1.1
3,631,955 - -0.4
3,632,190 + +0.0
3,632,190 + +0.1
3,632,190 + -1.3
3,632,190 + +0.0
3,632,193 + +0.5
3,632,193 + +0.9
3,632,193 + +1.6
3,632,193 + -1.3
3,632,381 - -1.0
3,632,522 - Pf1N1B4_3245 0.16 -1.6
3,633,143 - Pf1N1B4_3245 0.58 -2.1
3,633,215 - Pf1N1B4_3245 0.63 -1.3
3,633,281 - Pf1N1B4_3245 0.67 +1.0
3,633,281 - Pf1N1B4_3245 0.67 -2.6
3,633,338 - Pf1N1B4_3245 0.71 -1.3
3,633,770 - -1.0
3,633,770 - -0.7
3,633,770 - -0.6
3,634,037 - -1.0
3,634,132 - Pf1N1B4_3246 0.12 -1.5
3,634,318 - Pf1N1B4_3246 0.18 -2.9
3,634,442 + Pf1N1B4_3246 0.22 -3.4
3,634,594 - Pf1N1B4_3246 0.27 +1.0
3,634,594 - Pf1N1B4_3246 0.27 -0.6
3,634,635 + Pf1N1B4_3246 0.28 -1.8
3,634,636 - Pf1N1B4_3246 0.28 -1.2
3,634,640 - -1.3
3,634,640 - Pf1N1B4_3246 0.28 -1.6

Or see this region's nucleotide sequence