Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3024 and Pf1N1B4_3025 are separated by 175 nucleotides Pf1N1B4_3025 and Pf1N1B4_3026 overlap by 11 nucleotides Pf1N1B4_3026 and Pf1N1B4_3027 are separated by 1 nucleotides
Pf1N1B4_3024 - 4-aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 3,400,954 to 3,402,378
_3024
Pf1N1B4_3025 - Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1), at 3,402,554 to 3,403,378
_3025
Pf1N1B4_3026 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 3,403,368 to 3,404,315
_3026
Pf1N1B4_3027 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 3,404,317 to 3,405,435
_3027
Position (kb)
3403
3404
3405 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.382 kb on + strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.383 kb on - strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.403 kb on + strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.404 kb on - strand at 3402.486 kb on + strand at 3402.486 kb on + strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.487 kb on - strand at 3402.555 kb on + strand at 3402.600 kb on + strand at 3402.600 kb on + strand at 3402.600 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.603 kb on + strand at 3402.604 kb on - strand at 3402.825 kb on + strand, within Pf1N1B4_3025 at 3402.825 kb on + strand, within Pf1N1B4_3025 at 3402.882 kb on + strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.883 kb on - strand, within Pf1N1B4_3025 at 3402.918 kb on + strand, within Pf1N1B4_3025 at 3402.918 kb on + strand, within Pf1N1B4_3025 at 3402.919 kb on - strand, within Pf1N1B4_3025 at 3403.335 kb on - strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.366 kb on + strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.367 kb on - strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.411 kb on + strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.412 kb on - strand at 3403.463 kb on + strand, within Pf1N1B4_3026 at 3403.489 kb on + strand, within Pf1N1B4_3026 at 3403.490 kb on - strand, within Pf1N1B4_3026 at 3403.665 kb on + strand, within Pf1N1B4_3026 at 3403.666 kb on - strand, within Pf1N1B4_3026 at 3403.666 kb on - strand, within Pf1N1B4_3026 at 3403.732 kb on + strand, within Pf1N1B4_3026 at 3403.733 kb on - strand, within Pf1N1B4_3026 at 3403.733 kb on - strand, within Pf1N1B4_3026 at 3403.888 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.903 kb on + strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.904 kb on - strand, within Pf1N1B4_3026 at 3403.936 kb on + strand, within Pf1N1B4_3026 at 3403.967 kb on - strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand, within Pf1N1B4_3026 at 3403.978 kb on + strand at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.979 kb on - strand, within Pf1N1B4_3026 at 3403.982 kb on - strand, within Pf1N1B4_3026 at 3403.982 kb on - strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.140 kb on + strand, within Pf1N1B4_3026 at 3404.141 kb on - strand at 3404.179 kb on + strand, within Pf1N1B4_3026 at 3404.179 kb on + strand, within Pf1N1B4_3026 at 3404.227 kb on + strand at 3404.227 kb on + strand at 3404.227 kb on + strand at 3404.228 kb on - strand at 3404.228 kb on - strand at 3404.245 kb on + strand at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.687 kb on + strand, within Pf1N1B4_3027 at 3404.688 kb on - strand, within Pf1N1B4_3027 at 3404.964 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.076 kb on + strand, within Pf1N1B4_3027 at 3405.077 kb on - strand, within Pf1N1B4_3027 at 3405.077 kb on - strand, within Pf1N1B4_3027 at 3405.117 kb on + strand, within Pf1N1B4_3027 at 3405.117 kb on + strand at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.118 kb on - strand, within Pf1N1B4_3027 at 3405.168 kb on + strand, within Pf1N1B4_3027
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 3,402,382 + +0.4 3,402,382 + +3.1 3,402,382 + -0.3 3,402,382 + -1.2 3,402,382 + -0.8 3,402,382 + +0.8 3,402,383 - +0.7 3,402,383 - +0.4 3,402,383 - +0.5 3,402,383 - -0.9 3,402,383 - -0.3 3,402,383 - +1.1 3,402,403 + +0.2 3,402,403 + -0.9 3,402,403 + +0.8 3,402,403 + -0.6 3,402,403 + -0.1 3,402,403 + +0.1 3,402,403 + +1.8 3,402,403 + +0.1 3,402,403 + -1.4 3,402,403 + +1.7 3,402,403 + +0.8 3,402,403 + -1.4 3,402,403 + -0.8 3,402,403 + -0.6 3,402,403 + -0.5 3,402,403 + -0.6 3,402,403 + +1.0 3,402,404 - -0.1 3,402,404 - +0.1 3,402,404 - -0.7 3,402,404 - -0.2 3,402,404 - -1.2 3,402,404 - -0.5 3,402,404 - -1.1 3,402,404 - +0.1 3,402,404 - -0.8 3,402,404 - -0.2 3,402,404 - -0.5 3,402,404 - +2.1 3,402,404 - -0.9 3,402,404 - -1.5 3,402,404 - +0.4 3,402,486 + -0.6 3,402,486 + +0.7 3,402,487 - +0.1 3,402,487 - -1.2 3,402,487 - -1.7 3,402,487 - +0.8 3,402,555 + -0.1 3,402,600 + +0.5 3,402,600 + -0.1 3,402,600 + +0.5 3,402,603 + +0.3 3,402,603 + +1.6 3,402,603 + -0.3 3,402,603 + -1.0 3,402,604 - +1.5 3,402,825 + Pf1N1B4_3025 0.33 +0.1 3,402,825 + Pf1N1B4_3025 0.33 +0.3 3,402,882 + Pf1N1B4_3025 0.40 +0.6 3,402,883 - Pf1N1B4_3025 0.40 +2.1 3,402,883 - Pf1N1B4_3025 0.40 +1.7 3,402,883 - Pf1N1B4_3025 0.40 +1.0 3,402,883 - +2.7 3,402,883 - Pf1N1B4_3025 0.40 +1.1 3,402,883 - Pf1N1B4_3025 0.40 +0.1 3,402,918 + Pf1N1B4_3025 0.44 +1.7 3,402,918 + Pf1N1B4_3025 0.44 -0.6 3,402,919 - Pf1N1B4_3025 0.44 -0.6 3,403,335 - -1.2 3,403,366 + -1.0 3,403,366 + -1.9 3,403,366 + +0.4 3,403,367 - +0.5 3,403,367 - +0.8 3,403,367 - -0.2 3,403,411 + +1.5 3,403,411 + +1.0 3,403,411 + -0.5 3,403,411 + -0.6 3,403,411 + +1.5 3,403,411 + -1.2 3,403,412 - +0.1 3,403,412 - -0.5 3,403,412 - -0.9 3,403,412 - -1.1 3,403,463 + Pf1N1B4_3026 0.10 +0.9 3,403,489 + Pf1N1B4_3026 0.13 -1.5 3,403,490 - Pf1N1B4_3026 0.13 +0.4 3,403,665 + Pf1N1B4_3026 0.31 +1.4 3,403,666 - Pf1N1B4_3026 0.31 -0.1 3,403,666 - Pf1N1B4_3026 0.31 +0.2 3,403,732 + Pf1N1B4_3026 0.38 -1.9 3,403,733 - Pf1N1B4_3026 0.39 +1.1 3,403,733 - Pf1N1B4_3026 0.39 +0.7 3,403,888 + Pf1N1B4_3026 0.55 +0.6 3,403,903 + Pf1N1B4_3026 0.56 +2.4 3,403,903 + Pf1N1B4_3026 0.56 +0.1 3,403,903 + Pf1N1B4_3026 0.56 +1.1 3,403,904 - Pf1N1B4_3026 0.57 -0.1 3,403,904 - Pf1N1B4_3026 0.57 +0.1 3,403,904 - Pf1N1B4_3026 0.57 +1.1 3,403,936 + Pf1N1B4_3026 0.60 +0.0 3,403,967 - Pf1N1B4_3026 0.63 +1.4 3,403,978 + Pf1N1B4_3026 0.64 -0.3 3,403,978 + Pf1N1B4_3026 0.64 -1.5 3,403,978 + Pf1N1B4_3026 0.64 +0.1 3,403,978 + Pf1N1B4_3026 0.64 -0.7 3,403,978 + Pf1N1B4_3026 0.64 +0.1 3,403,978 + Pf1N1B4_3026 0.64 -1.9 3,403,978 + Pf1N1B4_3026 0.64 +1.1 3,403,978 + +0.1 3,403,979 - Pf1N1B4_3026 0.64 +0.7 3,403,979 - Pf1N1B4_3026 0.64 +1.5 3,403,979 - Pf1N1B4_3026 0.64 +0.0 3,403,979 - Pf1N1B4_3026 0.64 +1.3 3,403,979 - Pf1N1B4_3026 0.64 -1.2 3,403,982 - Pf1N1B4_3026 0.65 -0.2 3,403,982 - Pf1N1B4_3026 0.65 +0.4 3,404,140 + Pf1N1B4_3026 0.81 -2.1 3,404,140 + Pf1N1B4_3026 0.81 -0.1 3,404,140 + Pf1N1B4_3026 0.81 +0.9 3,404,140 + Pf1N1B4_3026 0.81 -1.5 3,404,141 - +0.5 3,404,179 + Pf1N1B4_3026 0.86 -0.4 3,404,179 + Pf1N1B4_3026 0.86 -1.5 3,404,227 + +0.5 3,404,227 + +0.1 3,404,227 + +0.1 3,404,228 - +1.1 3,404,228 - -2.2 3,404,245 + +2.3 3,404,687 + Pf1N1B4_3027 0.33 -0.3 3,404,687 + Pf1N1B4_3027 0.33 -2.2 3,404,687 + Pf1N1B4_3027 0.33 +1.5 3,404,687 + Pf1N1B4_3027 0.33 -1.1 3,404,688 - Pf1N1B4_3027 0.33 -0.7 3,404,964 + Pf1N1B4_3027 0.58 +0.0 3,405,076 + Pf1N1B4_3027 0.68 +0.1 3,405,076 + Pf1N1B4_3027 0.68 +0.5 3,405,076 + Pf1N1B4_3027 0.68 -0.2 3,405,077 - Pf1N1B4_3027 0.68 +3.1 3,405,077 - Pf1N1B4_3027 0.68 +2.4 3,405,117 + Pf1N1B4_3027 0.71 -0.6 3,405,117 + -0.9 3,405,118 - Pf1N1B4_3027 0.72 -0.4 3,405,118 - Pf1N1B4_3027 0.72 +0.1 3,405,118 - Pf1N1B4_3027 0.72 +0.9 3,405,118 - Pf1N1B4_3027 0.72 -0.9 3,405,168 + Pf1N1B4_3027 0.76 -0.9
Or see this region's nucleotide sequence