Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2893 and Pf1N1B4_2894 are separated by 7 nucleotides Pf1N1B4_2894 and Pf1N1B4_2895 are separated by 179 nucleotides Pf1N1B4_2895 and Pf1N1B4_2896 are separated by 242 nucleotides
Pf1N1B4_2893 - Carbonic anhydrase (EC 4.2.1.1), at 3,247,060 to 3,247,704
_2893
Pf1N1B4_2894 - FIG00956317: hypothetical protein, at 3,247,712 to 3,248,236
_2894
Pf1N1B4_2895 - FIG00953119: hypothetical protein, at 3,248,416 to 3,249,591
_2895
Pf1N1B4_2896 - L-Proline/Glycine betaine transporter ProP, at 3,249,834 to 3,251,186
_2896
Position (kb)
3248
3249
3250 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.441 kb on + strand, within Pf1N1B4_2893 at 3247.441 kb on + strand, within Pf1N1B4_2893 at 3247.442 kb on - strand, within Pf1N1B4_2893 at 3247.542 kb on + strand, within Pf1N1B4_2893 at 3247.542 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.676 kb on - strand at 3247.676 kb on - strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.983 kb on + strand, within Pf1N1B4_2894 at 3247.984 kb on - strand, within Pf1N1B4_2894 at 3247.984 kb on - strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.122 kb on - strand, within Pf1N1B4_2894 at 3248.174 kb on + strand, within Pf1N1B4_2894 at 3248.184 kb on + strand at 3248.184 kb on + strand at 3248.185 kb on - strand at 3248.185 kb on - strand at 3248.236 kb on + strand at 3248.236 kb on + strand at 3248.260 kb on + strand at 3248.260 kb on + strand at 3248.273 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.306 kb on - strand at 3248.306 kb on - strand at 3248.340 kb on + strand at 3248.350 kb on + strand at 3248.351 kb on - strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.418 kb on - strand at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand at 3248.596 kb on - strand, within Pf1N1B4_2895 at 3248.732 kb on + strand, within Pf1N1B4_2895 at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.733 kb on - strand at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.753 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.763 kb on - strand at 3248.763 kb on - strand, within Pf1N1B4_2895 at 3248.763 kb on - strand, within Pf1N1B4_2895 at 3248.763 kb on - strand, within Pf1N1B4_2895 at 3248.792 kb on - strand, within Pf1N1B4_2895 at 3248.852 kb on + strand, within Pf1N1B4_2895 at 3248.871 kb on + strand, within Pf1N1B4_2895 at 3248.871 kb on + strand, within Pf1N1B4_2895 at 3248.871 kb on + strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.875 kb on + strand, within Pf1N1B4_2895 at 3248.955 kb on - strand, within Pf1N1B4_2895 at 3248.955 kb on - strand, within Pf1N1B4_2895 at 3248.955 kb on - strand, within Pf1N1B4_2895 at 3248.966 kb on + strand, within Pf1N1B4_2895 at 3248.966 kb on + strand, within Pf1N1B4_2895 at 3248.966 kb on + strand at 3248.967 kb on - strand, within Pf1N1B4_2895 at 3248.967 kb on - strand, within Pf1N1B4_2895 at 3248.972 kb on + strand, within Pf1N1B4_2895 at 3248.972 kb on + strand, within Pf1N1B4_2895 at 3248.972 kb on + strand, within Pf1N1B4_2895 at 3248.973 kb on - strand, within Pf1N1B4_2895 at 3248.973 kb on - strand at 3249.119 kb on + strand, within Pf1N1B4_2895 at 3249.143 kb on + strand, within Pf1N1B4_2895 at 3249.143 kb on + strand, within Pf1N1B4_2895 at 3249.215 kb on + strand, within Pf1N1B4_2895 at 3249.223 kb on - strand, within Pf1N1B4_2895 at 3249.223 kb on - strand at 3249.223 kb on - strand, within Pf1N1B4_2895 at 3249.224 kb on + strand, within Pf1N1B4_2895 at 3249.224 kb on + strand, within Pf1N1B4_2895 at 3249.224 kb on + strand, within Pf1N1B4_2895 at 3249.224 kb on + strand, within Pf1N1B4_2895 at 3249.225 kb on - strand, within Pf1N1B4_2895 at 3249.225 kb on - strand, within Pf1N1B4_2895 at 3249.267 kb on - strand, within Pf1N1B4_2895 at 3249.312 kb on - strand, within Pf1N1B4_2895 at 3249.334 kb on - strand, within Pf1N1B4_2895 at 3249.341 kb on + strand, within Pf1N1B4_2895 at 3249.341 kb on + strand, within Pf1N1B4_2895 at 3249.342 kb on - strand, within Pf1N1B4_2895 at 3249.401 kb on - strand, within Pf1N1B4_2895 at 3249.401 kb on - strand, within Pf1N1B4_2895 at 3249.416 kb on + strand, within Pf1N1B4_2895 at 3249.416 kb on + strand, within Pf1N1B4_2895 at 3249.416 kb on + strand, within Pf1N1B4_2895 at 3249.417 kb on - strand, within Pf1N1B4_2895 at 3249.480 kb on - strand at 3249.546 kb on + strand at 3249.586 kb on + strand at 3249.587 kb on - strand at 3249.587 kb on - strand at 3249.634 kb on + strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.638 kb on + strand at 3249.638 kb on + strand at 3249.638 kb on + strand at 3249.638 kb on + strand at 3249.639 kb on - strand at 3249.639 kb on - strand at 3249.647 kb on + strand at 3249.647 kb on + strand at 3249.647 kb on + strand at 3249.647 kb on + strand at 3249.647 kb on + strand at 3249.648 kb on - strand at 3249.648 kb on - strand at 3249.828 kb on + strand at 3249.828 kb on + strand at 3249.829 kb on - strand at 3249.921 kb on + strand at 3249.921 kb on + strand at 3249.921 kb on + strand at 3249.921 kb on + strand at 3250.139 kb on + strand, within Pf1N1B4_2896 at 3250.209 kb on + strand, within Pf1N1B4_2896 at 3250.209 kb on + strand, within Pf1N1B4_2896 at 3250.225 kb on - strand, within Pf1N1B4_2896 at 3250.447 kb on - strand, within Pf1N1B4_2896 at 3250.504 kb on + strand, within Pf1N1B4_2896 at 3250.563 kb on - strand, within Pf1N1B4_2896
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 3,247,438 + Pf1N1B4_2893 0.59 +0.4 3,247,438 + Pf1N1B4_2893 0.59 -1.1 3,247,438 + Pf1N1B4_2893 0.59 -1.5 3,247,438 + Pf1N1B4_2893 0.59 +3.2 3,247,438 + Pf1N1B4_2893 0.59 -1.6 3,247,438 + Pf1N1B4_2893 0.59 -1.5 3,247,439 - Pf1N1B4_2893 0.59 +0.0 3,247,439 - Pf1N1B4_2893 0.59 -1.4 3,247,439 - Pf1N1B4_2893 0.59 +1.4 3,247,439 - Pf1N1B4_2893 0.59 -0.1 3,247,439 - Pf1N1B4_2893 0.59 -2.1 3,247,439 - Pf1N1B4_2893 0.59 +1.2 3,247,441 + Pf1N1B4_2893 0.59 -0.7 3,247,441 + Pf1N1B4_2893 0.59 +0.2 3,247,442 - Pf1N1B4_2893 0.59 +1.2 3,247,542 + Pf1N1B4_2893 0.75 +2.0 3,247,542 + Pf1N1B4_2893 0.75 -0.8 3,247,603 + Pf1N1B4_2893 0.84 -0.4 3,247,603 + Pf1N1B4_2893 0.84 -1.0 3,247,603 + -0.4 3,247,603 + Pf1N1B4_2893 0.84 +0.5 3,247,604 - Pf1N1B4_2893 0.84 -1.1 3,247,604 - Pf1N1B4_2893 0.84 -1.8 3,247,604 - Pf1N1B4_2893 0.84 +0.2 3,247,676 - -1.6 3,247,676 - +1.0 3,247,702 + +0.2 3,247,702 + -0.1 3,247,702 + -0.6 3,247,702 + +0.5 3,247,702 + +1.6 3,247,703 - -0.0 3,247,703 - +0.8 3,247,703 - -1.6 3,247,703 - +0.6 3,247,703 - +0.2 3,247,983 + Pf1N1B4_2894 0.52 +0.5 3,247,984 - Pf1N1B4_2894 0.52 -0.9 3,247,984 - Pf1N1B4_2894 0.52 +0.8 3,248,121 + Pf1N1B4_2894 0.78 -0.1 3,248,121 + Pf1N1B4_2894 0.78 +0.9 3,248,121 + Pf1N1B4_2894 0.78 -1.4 3,248,121 + Pf1N1B4_2894 0.78 +0.2 3,248,122 - Pf1N1B4_2894 0.78 -1.9 3,248,174 + Pf1N1B4_2894 0.88 +1.4 3,248,184 + +1.2 3,248,184 + +0.8 3,248,185 - +0.4 3,248,185 - +2.4 3,248,236 + -1.1 3,248,236 + +1.1 3,248,260 + +0.3 3,248,260 + -1.4 3,248,273 + +1.3 3,248,305 + -2.3 3,248,305 + -0.4 3,248,305 + +0.2 3,248,305 + +2.2 3,248,305 + +0.8 3,248,306 - -0.8 3,248,306 - +1.5 3,248,340 + -0.0 3,248,350 + +0.7 3,248,351 - -0.1 3,248,417 + -1.6 3,248,417 + -1.4 3,248,417 + +0.8 3,248,417 + -0.4 3,248,417 + -0.0 3,248,418 - -0.1 3,248,546 + Pf1N1B4_2895 0.11 -0.8 3,248,546 + Pf1N1B4_2895 0.11 +1.2 3,248,546 + Pf1N1B4_2895 0.11 -1.6 3,248,546 + Pf1N1B4_2895 0.11 -0.8 3,248,547 - Pf1N1B4_2895 0.11 +0.5 3,248,547 - Pf1N1B4_2895 0.11 -0.8 3,248,547 - Pf1N1B4_2895 0.11 +1.6 3,248,547 - Pf1N1B4_2895 0.11 -1.6 3,248,547 - Pf1N1B4_2895 0.11 -0.0 3,248,547 - +2.2 3,248,596 - Pf1N1B4_2895 0.15 -0.0 3,248,732 + Pf1N1B4_2895 0.27 -0.7 3,248,733 - Pf1N1B4_2895 0.27 +0.4 3,248,733 - +2.4 3,248,733 - Pf1N1B4_2895 0.27 -0.6 3,248,733 - Pf1N1B4_2895 0.27 +1.0 3,248,733 - Pf1N1B4_2895 0.27 +0.1 3,248,733 - Pf1N1B4_2895 0.27 +0.6 3,248,753 + Pf1N1B4_2895 0.29 -0.4 3,248,762 + Pf1N1B4_2895 0.29 +0.0 3,248,762 + Pf1N1B4_2895 0.29 -1.1 3,248,762 + Pf1N1B4_2895 0.29 +0.2 3,248,762 + Pf1N1B4_2895 0.29 -1.6 3,248,762 + Pf1N1B4_2895 0.29 -0.1 3,248,762 + Pf1N1B4_2895 0.29 -1.6 3,248,763 - -0.4 3,248,763 - Pf1N1B4_2895 0.30 -0.5 3,248,763 - Pf1N1B4_2895 0.30 -2.1 3,248,763 - Pf1N1B4_2895 0.30 -0.2 3,248,792 - Pf1N1B4_2895 0.32 +1.2 3,248,852 + Pf1N1B4_2895 0.37 +0.8 3,248,871 + Pf1N1B4_2895 0.39 -0.4 3,248,871 + Pf1N1B4_2895 0.39 +0.5 3,248,871 + Pf1N1B4_2895 0.39 +1.4 3,248,872 - Pf1N1B4_2895 0.39 -0.2 3,248,872 - Pf1N1B4_2895 0.39 -0.4 3,248,872 - Pf1N1B4_2895 0.39 +0.2 3,248,872 - Pf1N1B4_2895 0.39 -0.4 3,248,872 - Pf1N1B4_2895 0.39 -0.4 3,248,875 + Pf1N1B4_2895 0.39 +0.1 3,248,955 - Pf1N1B4_2895 0.46 -0.5 3,248,955 - Pf1N1B4_2895 0.46 -1.0 3,248,955 - Pf1N1B4_2895 0.46 -1.3 3,248,966 + Pf1N1B4_2895 0.47 +0.2 3,248,966 + Pf1N1B4_2895 0.47 -0.4 3,248,966 + +1.8 3,248,967 - Pf1N1B4_2895 0.47 -2.7 3,248,967 - Pf1N1B4_2895 0.47 +0.2 3,248,972 + Pf1N1B4_2895 0.47 +0.6 3,248,972 + Pf1N1B4_2895 0.47 +1.0 3,248,972 + Pf1N1B4_2895 0.47 +1.2 3,248,973 - Pf1N1B4_2895 0.47 -1.1 3,248,973 - +1.2 3,249,119 + Pf1N1B4_2895 0.60 -0.0 3,249,143 + Pf1N1B4_2895 0.62 -0.3 3,249,143 + Pf1N1B4_2895 0.62 +0.5 3,249,215 + Pf1N1B4_2895 0.68 -0.6 3,249,223 - Pf1N1B4_2895 0.69 -1.4 3,249,223 - +1.2 3,249,223 - Pf1N1B4_2895 0.69 -1.4 3,249,224 + Pf1N1B4_2895 0.69 -1.0 3,249,224 + Pf1N1B4_2895 0.69 -0.8 3,249,224 + Pf1N1B4_2895 0.69 -0.9 3,249,224 + Pf1N1B4_2895 0.69 +0.2 3,249,225 - Pf1N1B4_2895 0.69 +0.5 3,249,225 - Pf1N1B4_2895 0.69 -0.1 3,249,267 - Pf1N1B4_2895 0.72 -0.6 3,249,312 - Pf1N1B4_2895 0.76 -0.7 3,249,334 - Pf1N1B4_2895 0.78 -1.1 3,249,341 + Pf1N1B4_2895 0.79 -1.3 3,249,341 + Pf1N1B4_2895 0.79 +0.1 3,249,342 - Pf1N1B4_2895 0.79 -0.0 3,249,401 - Pf1N1B4_2895 0.84 +1.2 3,249,401 - Pf1N1B4_2895 0.84 +1.2 3,249,416 + Pf1N1B4_2895 0.85 -0.2 3,249,416 + Pf1N1B4_2895 0.85 -0.4 3,249,416 + Pf1N1B4_2895 0.85 -2.5 3,249,417 - Pf1N1B4_2895 0.85 +0.6 3,249,480 - +0.4 3,249,546 + +1.9 3,249,586 + +0.2 3,249,587 - +1.6 3,249,587 - +1.0 3,249,634 + -1.6 3,249,635 - +0.0 3,249,635 - +1.5 3,249,635 - -0.2 3,249,635 - -0.8 3,249,635 - +0.0 3,249,635 - +1.2 3,249,635 - +0.2 3,249,635 - +1.2 3,249,638 + -0.3 3,249,638 + -1.0 3,249,638 + -1.1 3,249,638 + -0.2 3,249,639 - +0.2 3,249,639 - +0.3 3,249,647 + +0.0 3,249,647 + -1.3 3,249,647 + +0.9 3,249,647 + +0.5 3,249,647 + -0.8 3,249,648 - +1.0 3,249,648 - -1.1 3,249,828 + -0.5 3,249,828 + +1.0 3,249,829 - +0.2 3,249,921 + +1.9 3,249,921 + +0.7 3,249,921 + +1.4 3,249,921 + -0.3 3,250,139 + Pf1N1B4_2896 0.23 +0.1 3,250,209 + Pf1N1B4_2896 0.28 +2.0 3,250,209 + Pf1N1B4_2896 0.28 +0.3 3,250,225 - Pf1N1B4_2896 0.29 +0.6 3,250,447 - Pf1N1B4_2896 0.45 +0.6 3,250,504 + Pf1N1B4_2896 0.50 -0.8 3,250,563 - Pf1N1B4_2896 0.54 -0.3
Or see this region's nucleotide sequence