Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2887 and Pf1N1B4_2888 overlap by 4 nucleotides Pf1N1B4_2888 and Pf1N1B4_2889 overlap by 11 nucleotides
Pf1N1B4_2887 - Chromosome segregation ATPases, at 3,240,173 to 3,243,013
_2887
Pf1N1B4_2888 - FIG00961017: hypothetical protein, at 3,243,010 to 3,243,711
_2888
Pf1N1B4_2889 - FIG00954491: hypothetical protein, at 3,243,701 to 3,245,044
_2889
Position (kb)
3243
3244 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3242.172 kb on + strand, within Pf1N1B4_2887 at 3242.173 kb on - strand, within Pf1N1B4_2887 at 3242.304 kb on + strand, within Pf1N1B4_2887 at 3242.738 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.799 kb on + strand at 3242.800 kb on - strand at 3242.800 kb on - strand at 3242.826 kb on + strand at 3242.826 kb on + strand at 3243.252 kb on - strand, within Pf1N1B4_2888 at 3243.252 kb on - strand, within Pf1N1B4_2888 at 3243.359 kb on + strand, within Pf1N1B4_2888 at 3243.359 kb on + strand, within Pf1N1B4_2888 at 3243.497 kb on - strand, within Pf1N1B4_2888 at 3243.497 kb on - strand at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.530 kb on + strand, within Pf1N1B4_2888 at 3243.531 kb on - strand, within Pf1N1B4_2888 at 3243.582 kb on + strand, within Pf1N1B4_2888 at 3243.583 kb on - strand, within Pf1N1B4_2888 at 3243.583 kb on - strand, within Pf1N1B4_2888 at 3243.697 kb on + strand at 3243.698 kb on - strand at 3243.698 kb on - strand at 3244.057 kb on - strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.063 kb on - strand, within Pf1N1B4_2889 at 3244.441 kb on - strand, within Pf1N1B4_2889 at 3244.441 kb on - strand, within Pf1N1B4_2889 at 3244.471 kb on - strand, within Pf1N1B4_2889 at 3244.644 kb on + strand, within Pf1N1B4_2889 at 3244.645 kb on - strand, within Pf1N1B4_2889 at 3244.674 kb on + strand, within Pf1N1B4_2889 at 3244.675 kb on - strand, within Pf1N1B4_2889 at 3244.675 kb on - strand, within Pf1N1B4_2889
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 3,242,172 + Pf1N1B4_2887 0.70 +0.8 3,242,173 - Pf1N1B4_2887 0.70 -0.2 3,242,304 + Pf1N1B4_2887 0.75 +2.2 3,242,738 + -1.6 3,242,778 + -0.6 3,242,778 + -0.7 3,242,778 + -0.4 3,242,778 + +0.2 3,242,778 + +0.3 3,242,779 - -0.2 3,242,779 - -0.8 3,242,779 - +0.0 3,242,779 - +2.2 3,242,779 - -0.8 3,242,779 - -0.2 3,242,779 - -0.3 3,242,779 - -0.1 3,242,779 - +2.0 3,242,779 - -1.1 3,242,799 + +0.2 3,242,800 - +0.7 3,242,800 - -0.8 3,242,826 + -0.1 3,242,826 + -0.1 3,243,252 - Pf1N1B4_2888 0.34 +1.2 3,243,252 - Pf1N1B4_2888 0.34 -1.0 3,243,359 + Pf1N1B4_2888 0.50 +0.4 3,243,359 + Pf1N1B4_2888 0.50 -1.4 3,243,497 - Pf1N1B4_2888 0.69 -1.1 3,243,497 - +0.2 3,243,528 - Pf1N1B4_2888 0.74 -0.2 3,243,528 - Pf1N1B4_2888 0.74 -1.2 3,243,528 - Pf1N1B4_2888 0.74 -0.4 3,243,530 + Pf1N1B4_2888 0.74 -1.7 3,243,531 - Pf1N1B4_2888 0.74 +0.6 3,243,582 + Pf1N1B4_2888 0.81 +0.3 3,243,583 - Pf1N1B4_2888 0.82 -1.4 3,243,583 - Pf1N1B4_2888 0.82 -1.8 3,243,697 + +0.2 3,243,698 - -1.4 3,243,698 - +0.4 3,244,057 - Pf1N1B4_2889 0.26 +0.6 3,244,062 + Pf1N1B4_2889 0.27 +1.2 3,244,062 + Pf1N1B4_2889 0.27 -0.9 3,244,062 + Pf1N1B4_2889 0.27 -0.2 3,244,063 - Pf1N1B4_2889 0.27 -0.1 3,244,441 - Pf1N1B4_2889 0.55 +0.9 3,244,441 - Pf1N1B4_2889 0.55 +0.2 3,244,471 - Pf1N1B4_2889 0.57 +0.2 3,244,644 + Pf1N1B4_2889 0.70 +0.3 3,244,645 - Pf1N1B4_2889 0.70 -0.2 3,244,674 + Pf1N1B4_2889 0.72 +1.2 3,244,675 - Pf1N1B4_2889 0.72 -0.6 3,244,675 - Pf1N1B4_2889 0.72 -0.6
Or see this region's nucleotide sequence