Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2870 and Pf1N1B4_2871 are separated by 231 nucleotides Pf1N1B4_2871 and Pf1N1B4_2872 are separated by 91 nucleotides Pf1N1B4_2872 and Pf1N1B4_2873 overlap by 4 nucleotides Pf1N1B4_2873 and Pf1N1B4_2874 are separated by 24 nucleotides
Pf1N1B4_2870 - Regulator of competence-specific genes, at 3,217,605 to 3,217,877
_2870
Pf1N1B4_2871 - FIG00954216: hypothetical protein, at 3,218,109 to 3,218,453
_2871
Pf1N1B4_2872 - FIG00955330: hypothetical protein, at 3,218,545 to 3,220,101
_2872
Pf1N1B4_2873 - hypothetical protein, at 3,220,098 to 3,220,244
_2873
Pf1N1B4_2874 - Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-), at 3,220,269 to 3,222,536
_2874
Position (kb)
3218
3219
3220
3221 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3217.561 kb on + strand at 3217.561 kb on + strand at 3217.561 kb on + strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.562 kb on - strand at 3217.588 kb on + strand at 3217.594 kb on + strand at 3217.594 kb on + strand at 3217.594 kb on + strand at 3217.595 kb on - strand at 3217.595 kb on - strand at 3217.595 kb on - strand at 3217.628 kb on + strand at 3217.629 kb on - strand at 3217.629 kb on - strand at 3217.629 kb on - strand at 3217.629 kb on - strand at 3217.630 kb on - strand at 3217.715 kb on - strand, within Pf1N1B4_2870 at 3217.715 kb on - strand, within Pf1N1B4_2870 at 3217.715 kb on - strand, within Pf1N1B4_2870 at 3217.929 kb on + strand at 3217.940 kb on - strand at 3217.984 kb on - strand at 3217.997 kb on + strand at 3217.998 kb on - strand at 3217.998 kb on - strand at 3218.052 kb on + strand at 3218.097 kb on - strand at 3218.188 kb on + strand, within Pf1N1B4_2871 at 3218.188 kb on + strand, within Pf1N1B4_2871 at 3218.189 kb on - strand, within Pf1N1B4_2871 at 3218.189 kb on - strand, within Pf1N1B4_2871 at 3218.278 kb on + strand, within Pf1N1B4_2871 at 3218.278 kb on + strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.279 kb on - strand, within Pf1N1B4_2871 at 3218.358 kb on - strand, within Pf1N1B4_2871 at 3218.358 kb on - strand, within Pf1N1B4_2871 at 3218.384 kb on + strand, within Pf1N1B4_2871 at 3218.419 kb on + strand at 3218.420 kb on - strand at 3218.538 kb on + strand at 3218.538 kb on + strand at 3218.546 kb on + strand at 3218.546 kb on + strand at 3218.709 kb on + strand, within Pf1N1B4_2872 at 3218.709 kb on + strand, within Pf1N1B4_2872 at 3218.709 kb on + strand, within Pf1N1B4_2872 at 3218.709 kb on + strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.710 kb on - strand, within Pf1N1B4_2872 at 3218.840 kb on + strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.841 kb on - strand, within Pf1N1B4_2872 at 3218.909 kb on + strand, within Pf1N1B4_2872 at 3218.909 kb on + strand, within Pf1N1B4_2872 at 3218.909 kb on + strand, within Pf1N1B4_2872 at 3218.910 kb on - strand, within Pf1N1B4_2872 at 3218.910 kb on - strand, within Pf1N1B4_2872 at 3218.910 kb on - strand, within Pf1N1B4_2872 at 3218.910 kb on - strand at 3218.935 kb on - strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.936 kb on + strand, within Pf1N1B4_2872 at 3218.937 kb on - strand, within Pf1N1B4_2872 at 3218.937 kb on - strand at 3218.937 kb on - strand, within Pf1N1B4_2872 at 3218.941 kb on - strand, within Pf1N1B4_2872 at 3218.941 kb on - strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.982 kb on + strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3218.983 kb on - strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.113 kb on + strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.114 kb on - strand, within Pf1N1B4_2872 at 3219.134 kb on + strand, within Pf1N1B4_2872 at 3219.160 kb on - strand, within Pf1N1B4_2872 at 3219.239 kb on + strand, within Pf1N1B4_2872 at 3219.266 kb on + strand, within Pf1N1B4_2872 at 3219.492 kb on + strand, within Pf1N1B4_2872 at 3219.870 kb on - strand, within Pf1N1B4_2872 at 3219.870 kb on - strand, within Pf1N1B4_2872 at 3219.981 kb on + strand at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.206 kb on + strand, within Pf1N1B4_2873 at 3220.208 kb on + strand, within Pf1N1B4_2873 at 3220.209 kb on - strand, within Pf1N1B4_2873 at 3220.795 kb on - strand, within Pf1N1B4_2874 at 3220.840 kb on - strand, within Pf1N1B4_2874 at 3221.010 kb on + strand, within Pf1N1B4_2874
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 3,217,561 + +0.6 3,217,561 + -0.3 3,217,561 + +0.1 3,217,562 - +0.0 3,217,562 - -1.1 3,217,562 - -1.4 3,217,562 - -0.1 3,217,562 - +0.2 3,217,562 - +0.7 3,217,562 - -0.3 3,217,588 + -0.5 3,217,594 + +1.6 3,217,594 + +0.8 3,217,594 + +0.1 3,217,595 - -0.6 3,217,595 - -1.0 3,217,595 - -0.5 3,217,628 + -0.8 3,217,629 - +1.8 3,217,629 - -1.4 3,217,629 - +0.8 3,217,629 - -0.2 3,217,630 - -0.4 3,217,715 - Pf1N1B4_2870 0.40 -0.8 3,217,715 - Pf1N1B4_2870 0.40 -0.3 3,217,715 - Pf1N1B4_2870 0.40 +1.2 3,217,929 + +1.0 3,217,940 - +0.7 3,217,984 - +1.8 3,217,997 + -0.6 3,217,998 - -0.9 3,217,998 - +0.8 3,218,052 + +1.5 3,218,097 - +0.5 3,218,188 + Pf1N1B4_2871 0.23 -0.4 3,218,188 + Pf1N1B4_2871 0.23 -0.4 3,218,189 - Pf1N1B4_2871 0.23 +0.5 3,218,189 - Pf1N1B4_2871 0.23 -0.3 3,218,278 + Pf1N1B4_2871 0.49 -0.3 3,218,278 + Pf1N1B4_2871 0.49 +0.4 3,218,279 - Pf1N1B4_2871 0.49 +0.3 3,218,279 - Pf1N1B4_2871 0.49 -0.0 3,218,279 - Pf1N1B4_2871 0.49 +1.1 3,218,279 - Pf1N1B4_2871 0.49 +1.6 3,218,279 - Pf1N1B4_2871 0.49 -1.6 3,218,358 - Pf1N1B4_2871 0.72 +1.2 3,218,358 - Pf1N1B4_2871 0.72 +0.9 3,218,384 + Pf1N1B4_2871 0.80 -1.4 3,218,419 + +0.7 3,218,420 - -0.8 3,218,538 + -0.2 3,218,538 + -0.4 3,218,546 + -1.2 3,218,546 + +1.5 3,218,709 + Pf1N1B4_2872 0.11 -2.1 3,218,709 + Pf1N1B4_2872 0.11 -1.2 3,218,709 + Pf1N1B4_2872 0.11 -1.7 3,218,709 + Pf1N1B4_2872 0.11 -1.6 3,218,710 - Pf1N1B4_2872 0.11 -0.0 3,218,710 - Pf1N1B4_2872 0.11 -1.4 3,218,710 - Pf1N1B4_2872 0.11 -0.4 3,218,710 - Pf1N1B4_2872 0.11 -1.7 3,218,710 - Pf1N1B4_2872 0.11 -3.6 3,218,710 - Pf1N1B4_2872 0.11 -0.7 3,218,710 - Pf1N1B4_2872 0.11 -0.8 3,218,710 - Pf1N1B4_2872 0.11 -2.5 3,218,840 + Pf1N1B4_2872 0.19 +1.6 3,218,841 - Pf1N1B4_2872 0.19 -0.1 3,218,841 - Pf1N1B4_2872 0.19 -0.3 3,218,841 - Pf1N1B4_2872 0.19 +0.6 3,218,841 - Pf1N1B4_2872 0.19 +1.2 3,218,841 - Pf1N1B4_2872 0.19 -0.0 3,218,841 - Pf1N1B4_2872 0.19 -0.8 3,218,909 + Pf1N1B4_2872 0.23 -0.8 3,218,909 + Pf1N1B4_2872 0.23 -0.4 3,218,909 + Pf1N1B4_2872 0.23 +0.2 3,218,910 - Pf1N1B4_2872 0.23 +0.6 3,218,910 - Pf1N1B4_2872 0.23 -0.0 3,218,910 - Pf1N1B4_2872 0.23 -0.8 3,218,910 - -0.1 3,218,935 - Pf1N1B4_2872 0.25 -0.3 3,218,936 + Pf1N1B4_2872 0.25 -0.2 3,218,936 + Pf1N1B4_2872 0.25 +0.5 3,218,936 + Pf1N1B4_2872 0.25 -0.4 3,218,936 + Pf1N1B4_2872 0.25 +1.8 3,218,936 + Pf1N1B4_2872 0.25 -1.1 3,218,936 + +1.2 3,218,936 + Pf1N1B4_2872 0.25 -0.7 3,218,936 + Pf1N1B4_2872 0.25 +0.5 3,218,936 + Pf1N1B4_2872 0.25 -2.3 3,218,937 - Pf1N1B4_2872 0.25 +1.2 3,218,937 - +0.6 3,218,937 - Pf1N1B4_2872 0.25 -1.0 3,218,941 - Pf1N1B4_2872 0.25 -1.6 3,218,941 - Pf1N1B4_2872 0.25 +2.2 3,218,982 + Pf1N1B4_2872 0.28 +0.3 3,218,982 + Pf1N1B4_2872 0.28 -0.1 3,218,982 + Pf1N1B4_2872 0.28 -1.3 3,218,982 + Pf1N1B4_2872 0.28 -0.2 3,218,982 + Pf1N1B4_2872 0.28 +0.2 3,218,982 + Pf1N1B4_2872 0.28 -0.2 3,218,982 + Pf1N1B4_2872 0.28 +0.2 3,218,982 + Pf1N1B4_2872 0.28 -0.5 3,218,982 + Pf1N1B4_2872 0.28 +0.2 3,218,983 - Pf1N1B4_2872 0.28 -2.0 3,218,983 - Pf1N1B4_2872 0.28 -1.1 3,218,983 - Pf1N1B4_2872 0.28 +0.2 3,218,983 - Pf1N1B4_2872 0.28 +0.4 3,218,983 - Pf1N1B4_2872 0.28 -1.3 3,218,983 - Pf1N1B4_2872 0.28 -0.7 3,218,983 - Pf1N1B4_2872 0.28 -1.6 3,218,983 - Pf1N1B4_2872 0.28 -0.8 3,218,983 - Pf1N1B4_2872 0.28 -0.6 3,218,983 - Pf1N1B4_2872 0.28 -2.8 3,218,983 - Pf1N1B4_2872 0.28 -1.3 3,218,983 - Pf1N1B4_2872 0.28 -1.4 3,218,983 - Pf1N1B4_2872 0.28 -2.0 3,218,983 - Pf1N1B4_2872 0.28 +1.2 3,218,983 - Pf1N1B4_2872 0.28 +0.2 3,219,113 + Pf1N1B4_2872 0.36 -1.3 3,219,113 + Pf1N1B4_2872 0.36 -1.1 3,219,113 + Pf1N1B4_2872 0.36 -0.8 3,219,113 + Pf1N1B4_2872 0.36 -1.1 3,219,113 + Pf1N1B4_2872 0.36 -1.4 3,219,113 + Pf1N1B4_2872 0.36 -1.4 3,219,114 - Pf1N1B4_2872 0.37 -1.0 3,219,114 - Pf1N1B4_2872 0.37 -3.3 3,219,114 - Pf1N1B4_2872 0.37 -2.0 3,219,114 - Pf1N1B4_2872 0.37 -1.3 3,219,114 - Pf1N1B4_2872 0.37 -1.9 3,219,114 - Pf1N1B4_2872 0.37 -1.1 3,219,114 - Pf1N1B4_2872 0.37 -1.9 3,219,114 - Pf1N1B4_2872 0.37 -1.3 3,219,114 - Pf1N1B4_2872 0.37 -2.3 3,219,114 - Pf1N1B4_2872 0.37 -1.8 3,219,134 + Pf1N1B4_2872 0.38 +0.5 3,219,160 - Pf1N1B4_2872 0.39 +0.5 3,219,239 + Pf1N1B4_2872 0.45 -2.4 3,219,266 + Pf1N1B4_2872 0.46 -1.2 3,219,492 + Pf1N1B4_2872 0.61 +0.1 3,219,870 - Pf1N1B4_2872 0.85 -0.8 3,219,870 - Pf1N1B4_2872 0.85 -1.5 3,219,981 + -1.1 3,220,206 + Pf1N1B4_2873 0.73 -1.6 3,220,206 + Pf1N1B4_2873 0.73 -0.9 3,220,206 + Pf1N1B4_2873 0.73 +0.2 3,220,206 + Pf1N1B4_2873 0.73 -1.1 3,220,206 + Pf1N1B4_2873 0.73 +0.2 3,220,208 + Pf1N1B4_2873 0.75 +0.2 3,220,209 - Pf1N1B4_2873 0.76 -1.1 3,220,795 - Pf1N1B4_2874 0.23 +0.3 3,220,840 - Pf1N1B4_2874 0.25 -0.8 3,221,010 + Pf1N1B4_2874 0.33 -0.8
Or see this region's nucleotide sequence