Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2793 and Pf1N1B4_2794 are separated by 143 nucleotides Pf1N1B4_2794 and Pf1N1B4_2795 are separated by 205 nucleotides Pf1N1B4_2795 and Pf1N1B4_2796 are separated by 154 nucleotides
Pf1N1B4_2793 - FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8), at 3,138,232 to 3,138,849
_2793
Pf1N1B4_2794 - FIG00953595: hypothetical protein, at 3,138,993 to 3,139,250
_2794
Pf1N1B4_2795 - Alkylphosphonate utilization operon protein PhnA, at 3,139,456 to 3,139,797
_2795
Pf1N1B4_2796 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29), at 3,139,952 to 3,140,920
_2796
Position (kb)
3139
3140 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3138.611 kb on - strand, within Pf1N1B4_2793 at 3138.652 kb on + strand, within Pf1N1B4_2793 at 3138.653 kb on - strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.751 kb on + strand, within Pf1N1B4_2793 at 3138.752 kb on - strand at 3138.752 kb on - strand, within Pf1N1B4_2793 at 3138.752 kb on - strand, within Pf1N1B4_2793 at 3138.752 kb on - strand, within Pf1N1B4_2793 at 3138.752 kb on - strand, within Pf1N1B4_2793 at 3138.812 kb on + strand at 3138.813 kb on - strand at 3138.813 kb on - strand at 3138.818 kb on + strand at 3138.847 kb on + strand at 3138.848 kb on - strand at 3138.848 kb on - strand at 3138.997 kb on - strand at 3138.997 kb on - strand at 3138.997 kb on - strand at 3139.045 kb on + strand, within Pf1N1B4_2794 at 3139.046 kb on - strand, within Pf1N1B4_2794 at 3139.105 kb on - strand, within Pf1N1B4_2794 at 3139.164 kb on + strand, within Pf1N1B4_2794 at 3139.165 kb on - strand, within Pf1N1B4_2794 at 3139.207 kb on + strand, within Pf1N1B4_2794 at 3139.207 kb on + strand, within Pf1N1B4_2794 at 3139.208 kb on - strand, within Pf1N1B4_2794 at 3139.243 kb on + strand at 3139.243 kb on + strand at 3139.243 kb on + strand at 3139.243 kb on + strand at 3139.243 kb on + strand at 3139.244 kb on - strand at 3139.244 kb on - strand at 3139.244 kb on - strand at 3139.244 kb on - strand at 3139.244 kb on - strand at 3139.244 kb on - strand at 3139.244 kb on - strand at 3139.244 kb on - strand at 3139.314 kb on + strand at 3139.751 kb on + strand, within Pf1N1B4_2795 at 3139.751 kb on + strand, within Pf1N1B4_2795 at 3139.752 kb on - strand, within Pf1N1B4_2795 at 3139.752 kb on - strand, within Pf1N1B4_2795 at 3139.752 kb on - strand, within Pf1N1B4_2795 at 3139.752 kb on - strand, within Pf1N1B4_2795 at 3139.752 kb on - strand, within Pf1N1B4_2795 at 3139.752 kb on - strand, within Pf1N1B4_2795 at 3139.752 kb on - strand, within Pf1N1B4_2795 at 3139.752 kb on - strand, within Pf1N1B4_2795 at 3139.757 kb on + strand, within Pf1N1B4_2795 at 3139.757 kb on + strand, within Pf1N1B4_2795 at 3139.757 kb on + strand, within Pf1N1B4_2795 at 3139.758 kb on - strand, within Pf1N1B4_2795 at 3139.758 kb on - strand, within Pf1N1B4_2795 at 3139.799 kb on - strand at 3139.839 kb on + strand at 3139.839 kb on + strand at 3139.839 kb on + strand at 3139.839 kb on + strand at 3139.839 kb on + strand at 3139.839 kb on + strand at 3139.839 kb on + strand at 3139.839 kb on + strand at 3139.840 kb on - strand at 3139.840 kb on - strand at 3139.840 kb on - strand at 3139.840 kb on - strand at 3139.840 kb on - strand at 3139.841 kb on + strand at 3139.920 kb on + strand at 3139.921 kb on - strand at 3139.921 kb on - strand at 3139.954 kb on - strand at 3139.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 3,138,611 - Pf1N1B4_2793 0.61 -1.4 3,138,652 + Pf1N1B4_2793 0.68 -1.2 3,138,653 - Pf1N1B4_2793 0.68 +1.2 3,138,751 + Pf1N1B4_2793 0.84 -0.1 3,138,751 + Pf1N1B4_2793 0.84 +0.6 3,138,751 + Pf1N1B4_2793 0.84 -0.2 3,138,751 + Pf1N1B4_2793 0.84 -2.2 3,138,751 + Pf1N1B4_2793 0.84 +0.4 3,138,752 - -1.1 3,138,752 - Pf1N1B4_2793 0.84 +0.2 3,138,752 - Pf1N1B4_2793 0.84 -0.2 3,138,752 - Pf1N1B4_2793 0.84 +1.5 3,138,752 - Pf1N1B4_2793 0.84 -0.9 3,138,812 + -0.8 3,138,813 - +0.6 3,138,813 - -0.7 3,138,818 + -0.5 3,138,847 + -0.8 3,138,848 - -0.4 3,138,848 - -1.4 3,138,997 - +1.5 3,138,997 - -0.8 3,138,997 - -2.1 3,139,045 + Pf1N1B4_2794 0.20 -0.2 3,139,046 - Pf1N1B4_2794 0.21 +1.8 3,139,105 - Pf1N1B4_2794 0.43 -0.4 3,139,164 + Pf1N1B4_2794 0.66 +0.8 3,139,165 - Pf1N1B4_2794 0.67 -0.2 3,139,207 + Pf1N1B4_2794 0.83 +1.2 3,139,207 + Pf1N1B4_2794 0.83 +2.2 3,139,208 - Pf1N1B4_2794 0.83 +1.8 3,139,243 + -0.8 3,139,243 + -0.8 3,139,243 + -0.4 3,139,243 + +0.6 3,139,243 + -1.1 3,139,244 - -0.2 3,139,244 - -1.4 3,139,244 - -0.5 3,139,244 - +2.6 3,139,244 - -0.9 3,139,244 - -0.5 3,139,244 - +0.4 3,139,244 - +0.9 3,139,314 + +1.2 3,139,751 + Pf1N1B4_2795 0.86 -0.8 3,139,751 + Pf1N1B4_2795 0.86 -0.1 3,139,752 - Pf1N1B4_2795 0.87 -1.1 3,139,752 - Pf1N1B4_2795 0.87 -1.6 3,139,752 - Pf1N1B4_2795 0.87 -0.2 3,139,752 - Pf1N1B4_2795 0.87 -0.1 3,139,752 - Pf1N1B4_2795 0.87 +0.2 3,139,752 - Pf1N1B4_2795 0.87 +4.4 3,139,752 - Pf1N1B4_2795 0.87 +1.2 3,139,752 - Pf1N1B4_2795 0.87 -1.8 3,139,757 + Pf1N1B4_2795 0.88 -0.8 3,139,757 + Pf1N1B4_2795 0.88 -0.7 3,139,757 + Pf1N1B4_2795 0.88 -0.8 3,139,758 - Pf1N1B4_2795 0.88 -1.8 3,139,758 - Pf1N1B4_2795 0.88 -1.4 3,139,799 - -0.0 3,139,839 + +1.8 3,139,839 + -1.8 3,139,839 + +1.5 3,139,839 + +0.2 3,139,839 + -0.4 3,139,839 + -1.1 3,139,839 + -0.4 3,139,839 + -0.0 3,139,840 - -0.1 3,139,840 - +1.2 3,139,840 - -0.4 3,139,840 - +1.2 3,139,840 - -1.0 3,139,841 + +0.5 3,139,920 + -0.1 3,139,921 - +0.4 3,139,921 - +2.2 3,139,954 - -1.9 3,139,954 - -0.2
Or see this region's nucleotide sequence