Experiment: Spectinomycin 0.05 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_2672 and Pf1N1B4_2673 are separated by 265 nucleotides Pf1N1B4_2673 and Pf1N1B4_2674 are separated by 103 nucleotides Pf1N1B4_2674 and Pf1N1B4_2675 overlap by 15 nucleotides  
        
        Pf1N1B4_2672 - Ethanolamine permease, at 3,008,802 to 3,010,172 
         
        _2672 
         
        
        Pf1N1B4_2673 - Aldehyde dehydrogenase (EC 1.2.1.3), at 3,010,438 to 3,011,958 
         
        _2673 
         
        
        Pf1N1B4_2674 - hypothetical protein, at 3,012,062 to 3,012,424 
         
        _2674 
         
        
        Pf1N1B4_2675 - Nitrogen regulation protein NR(I), at 3,012,410 to 3,014,323 
         
        _2675 
         Position (kb)  
3010 
 
3011 
 
3012 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 
 
3 
 
4 at 3009.484 kb on + strand, within Pf1N1B4_2672 at 3009.484 kb on + strand, within Pf1N1B4_2672 at 3009.484 kb on + strand, within Pf1N1B4_2672 at 3009.485 kb on - strand, within Pf1N1B4_2672 at 3009.485 kb on - strand, within Pf1N1B4_2672 at 3009.485 kb on - strand, within Pf1N1B4_2672 at 3009.485 kb on - strand, within Pf1N1B4_2672 at 3009.577 kb on - strand, within Pf1N1B4_2672 at 3009.577 kb on - strand, within Pf1N1B4_2672 at 3009.607 kb on - strand, within Pf1N1B4_2672 at 3009.607 kb on - strand, within Pf1N1B4_2672 at 3009.759 kb on - strand, within Pf1N1B4_2672 at 3009.782 kb on - strand, within Pf1N1B4_2672 at 3009.835 kb on + strand, within Pf1N1B4_2672 at 3009.835 kb on + strand, within Pf1N1B4_2672 at 3009.836 kb on - strand, within Pf1N1B4_2672 at 3009.836 kb on - strand, within Pf1N1B4_2672 at 3009.941 kb on - strand, within Pf1N1B4_2672 at 3009.998 kb on + strand, within Pf1N1B4_2672 at 3009.998 kb on + strand, within Pf1N1B4_2672 at 3009.999 kb on - strand, within Pf1N1B4_2672 at 3009.999 kb on - strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.010 kb on - strand, within Pf1N1B4_2672 at 3010.010 kb on - strand, within Pf1N1B4_2672 at 3010.010 kb on - strand, within Pf1N1B4_2672 at 3010.088 kb on - strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.160 kb on + strand at 3010.160 kb on + strand at 3010.160 kb on + strand at 3010.160 kb on + strand at 3010.160 kb on + strand at 3010.161 kb on - strand at 3010.161 kb on - strand at 3010.161 kb on - strand at 3010.163 kb on + strand at 3010.163 kb on + strand at 3010.163 kb on + strand at 3010.164 kb on - strand at 3010.164 kb on - strand at 3010.164 kb on - strand at 3010.172 kb on + strand at 3010.172 kb on + strand at 3010.172 kb on + strand at 3010.172 kb on + strand at 3010.172 kb on + strand at 3010.173 kb on - strand at 3010.173 kb on - strand at 3010.173 kb on - strand at 3010.173 kb on - strand at 3010.193 kb on - strand at 3010.193 kb on - strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.281 kb on - strand at 3010.281 kb on - strand at 3010.291 kb on + strand at 3010.336 kb on + strand at 3010.417 kb on + strand at 3010.417 kb on + strand at 3010.417 kb on + strand at 3010.418 kb on - strand at 3010.418 kb on - strand at 3010.466 kb on + strand at 3010.467 kb on - strand at 3010.467 kb on - strand at 3010.467 kb on - strand at 3010.548 kb on - strand at 3010.583 kb on + strand at 3010.728 kb on + strand, within Pf1N1B4_2673 at 3010.729 kb on - strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.811 kb on + strand, within Pf1N1B4_2673 at 3010.895 kb on + strand, within Pf1N1B4_2673 at 3011.019 kb on - strand, within Pf1N1B4_2673 at 3011.274 kb on + strand, within Pf1N1B4_2673 at 3011.412 kb on + strand, within Pf1N1B4_2673 at 3011.514 kb on - strand, within Pf1N1B4_2673 at 3011.820 kb on + strand at 3011.820 kb on + strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.845 kb on - strand at 3011.845 kb on - strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.899 kb on - strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.904 kb on - strand at 3011.904 kb on - strand at 3011.904 kb on - strand at 3011.951 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.989 kb on - strand at 3012.016 kb on + strand at 3012.023 kb on - strand at 3012.058 kb on + strand at 3012.233 kb on + strand, within Pf1N1B4_2674 at 3012.234 kb on - strand, within Pf1N1B4_2674 at 3012.372 kb on - strand, within Pf1N1B4_2674 at 3012.401 kb on + strand at 3012.404 kb on + strand at 3012.404 kb on + strand at 3012.404 kb on + strand at 3012.405 kb on - strand at 3012.405 kb on - strand at 3012.405 kb on - strand at 3012.536 kb on + strand at 3012.537 kb on - strand at 3012.537 kb on - strand at 3012.537 kb on - strand at 3012.806 kb on - strand, within Pf1N1B4_2675 at 3012.883 kb on + strand, within Pf1N1B4_2675 at 3012.884 kb on - strand, within Pf1N1B4_2675  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Spectinomycin 0.05 mg/ml             remove   3,009,484   +    Pf1N1B4_2672   0.50  -0.0   3,009,484   +    Pf1N1B4_2672   0.50  +0.0   3,009,484   +    Pf1N1B4_2672   0.50  +1.0   3,009,485   -    Pf1N1B4_2672   0.50  -1.0   3,009,485   -    Pf1N1B4_2672   0.50  -0.3   3,009,485   -    Pf1N1B4_2672   0.50  -0.4   3,009,485   -    Pf1N1B4_2672   0.50  -0.5   3,009,577   -    Pf1N1B4_2672   0.57  -0.7   3,009,577   -    Pf1N1B4_2672   0.57  +0.8   3,009,607   -    Pf1N1B4_2672   0.59  -1.3   3,009,607   -    Pf1N1B4_2672   0.59  +0.1   3,009,759   -    Pf1N1B4_2672   0.70  +0.9   3,009,782   -    Pf1N1B4_2672   0.71  +1.4   3,009,835   +    Pf1N1B4_2672   0.75  -1.6   3,009,835   +    Pf1N1B4_2672   0.75  -0.1   3,009,836   -    Pf1N1B4_2672   0.75  +0.5   3,009,836   -    Pf1N1B4_2672   0.75  +1.0   3,009,941   -    Pf1N1B4_2672   0.83  -0.7   3,009,998   +    Pf1N1B4_2672   0.87  -1.9   3,009,998   +    Pf1N1B4_2672   0.87  -1.0   3,009,999   -    Pf1N1B4_2672   0.87  +0.5   3,009,999   -    Pf1N1B4_2672   0.87  -0.1   3,010,009   +    Pf1N1B4_2672   0.88  +0.3   3,010,009   +        +0.7   3,010,009   +    Pf1N1B4_2672   0.88  +1.1   3,010,009   +    Pf1N1B4_2672   0.88  -0.6   3,010,009   +    Pf1N1B4_2672   0.88  +0.1   3,010,009   +    Pf1N1B4_2672   0.88  -1.0   3,010,009   +    Pf1N1B4_2672   0.88  +1.6   3,010,009   +    Pf1N1B4_2672   0.88  -3.5   3,010,009   +    Pf1N1B4_2672   0.88  +1.7   3,010,010   -    Pf1N1B4_2672   0.88  +0.1   3,010,010   -    Pf1N1B4_2672   0.88  -0.7   3,010,010   -    Pf1N1B4_2672   0.88  +0.3   3,010,088   -        -0.1   3,010,125   +        +1.0   3,010,125   +        -3.2   3,010,125   +        +0.6   3,010,125   +        +0.5   3,010,125   +        -0.3   3,010,125   +        -0.1   3,010,125   +        +0.0   3,010,125   +        +0.3   3,010,126   -        +2.5   3,010,126   -        -0.3   3,010,126   -        -1.0   3,010,126   -        +1.0   3,010,126   -        -0.7   3,010,126   -        -0.0   3,010,160   +        +0.9   3,010,160   +        +0.1   3,010,160   +        +0.3   3,010,160   +        +0.1   3,010,160   +        -0.3   3,010,161   -        +3.3   3,010,161   -        +0.7   3,010,161   -        -0.0   3,010,163   +        -1.9   3,010,163   +        +1.1   3,010,163   +        +0.1   3,010,164   -        -0.1   3,010,164   -        -0.7   3,010,164   -        -1.7   3,010,172   +        -0.5   3,010,172   +        +1.3   3,010,172   +        -0.1   3,010,172   +        +1.6   3,010,172   +        +1.2   3,010,173   -        -0.7   3,010,173   -        +1.9   3,010,173   -        +0.8   3,010,173   -        +1.0   3,010,193   -        -1.6   3,010,193   -        +1.3   3,010,280   +        +1.7   3,010,280   +        -0.7   3,010,280   +        +0.2   3,010,280   +        +0.3   3,010,280   +        -1.0   3,010,280   +        +0.5   3,010,281   -        -0.3   3,010,281   -        +0.5   3,010,291   +        -0.7   3,010,336   +        -0.7   3,010,417   +        -0.1   3,010,417   +        +0.3   3,010,417   +        -0.7   3,010,418   -        -0.8   3,010,418   -        -1.2   3,010,466   +        -1.3   3,010,467   -        -0.3   3,010,467   -        -0.0   3,010,467   -        +0.2   3,010,548   -        +1.3   3,010,583   +        +2.3   3,010,728   +    Pf1N1B4_2673   0.19  -1.9   3,010,729   -    Pf1N1B4_2673   0.19  +0.6   3,010,808   +    Pf1N1B4_2673   0.24  -0.7   3,010,808   +    Pf1N1B4_2673   0.24  -1.2   3,010,808   +    Pf1N1B4_2673   0.24  +0.9   3,010,808   +    Pf1N1B4_2673   0.24  -0.3   3,010,808   +    Pf1N1B4_2673   0.24  +0.6   3,010,808   +    Pf1N1B4_2673   0.24  +0.3   3,010,808   +    Pf1N1B4_2673   0.24  +1.0   3,010,808   +    Pf1N1B4_2673   0.24  -0.3   3,010,808   +    Pf1N1B4_2673   0.24  -2.7   3,010,808   +    Pf1N1B4_2673   0.24  -0.1   3,010,808   +    Pf1N1B4_2673   0.24  +1.7   3,010,808   +    Pf1N1B4_2673   0.24  +4.3   3,010,808   +    Pf1N1B4_2673   0.24  +0.7   3,010,808   +    Pf1N1B4_2673   0.24  +1.1   3,010,809   -    Pf1N1B4_2673   0.24  +0.3   3,010,809   -    Pf1N1B4_2673   0.24  -0.1   3,010,809   -    Pf1N1B4_2673   0.24  -1.7   3,010,809   -    Pf1N1B4_2673   0.24  -0.3   3,010,809   -    Pf1N1B4_2673   0.24  +0.8   3,010,809   -    Pf1N1B4_2673   0.24  +1.3   3,010,809   -    Pf1N1B4_2673   0.24  -0.2   3,010,809   -    Pf1N1B4_2673   0.24  +1.3   3,010,809   -    Pf1N1B4_2673   0.24  -1.3   3,010,809   -    Pf1N1B4_2673   0.24  +1.7   3,010,809   -    Pf1N1B4_2673   0.24  +0.6   3,010,811   +    Pf1N1B4_2673   0.25  -0.6   3,010,895   +    Pf1N1B4_2673   0.30  -1.3   3,011,019   -    Pf1N1B4_2673   0.38  +1.3   3,011,274   +    Pf1N1B4_2673   0.55  -0.7   3,011,412   +    Pf1N1B4_2673   0.64  +1.3   3,011,514   -    Pf1N1B4_2673   0.71  +1.1   3,011,820   +        +3.1   3,011,820   +        +0.7   3,011,821   -        -0.9   3,011,821   -        +0.1   3,011,821   -        +0.1   3,011,821   -        -0.1   3,011,821   -        -1.9   3,011,821   -        -1.9   3,011,845   -        +0.3   3,011,845   -        -2.0   3,011,894   +        +2.1   3,011,894   +        -1.4   3,011,894   +        +0.7   3,011,894   +        +0.3   3,011,894   +        +0.9   3,011,894   +        -0.3   3,011,894   +        +0.9   3,011,894   +        +0.3   3,011,894   +        -0.3   3,011,894   +        +0.4   3,011,894   +        +1.9   3,011,895   -        -0.9   3,011,895   -        -1.3   3,011,895   -        -0.7   3,011,895   -        -1.3   3,011,895   -        +0.5   3,011,895   -        +0.1   3,011,895   -        -0.7   3,011,895   -        -2.3   3,011,899   -        +0.5   3,011,903   +        +0.7   3,011,903   +        -1.0   3,011,903   +        -0.0   3,011,903   +        -1.1   3,011,903   +        -1.0   3,011,903   +        +0.9   3,011,903   +        -2.5   3,011,904   -        +2.3   3,011,904   -        +0.1   3,011,904   -        +0.4   3,011,951   +        -0.7   3,011,986   +        +1.0   3,011,986   +        -1.4   3,011,986   +        +0.1   3,011,986   +        +1.0   3,011,986   +        -0.3   3,011,986   +        +3.6   3,011,986   +        +1.5   3,011,987   -        +0.6   3,011,987   -        -1.0   3,011,987   -        -1.3   3,011,987   -        -0.0   3,011,988   +        +0.3   3,011,988   +        -0.8   3,011,988   +        -1.5   3,011,988   +        -0.2   3,011,989   -        -2.0   3,012,016   +        -0.4   3,012,023   -        +2.0   3,012,058   +        -1.0   3,012,233   +    Pf1N1B4_2674   0.47  +0.3   3,012,234   -    Pf1N1B4_2674   0.47  +0.8   3,012,372   -    Pf1N1B4_2674   0.85  +0.9   3,012,401   +        +0.3   3,012,404   +        -1.0   3,012,404   +        -0.8   3,012,404   +        +1.3   3,012,405   -        -0.7   3,012,405   -        -0.5   3,012,405   -        -1.0   3,012,536   +        +1.5   3,012,537   -        -0.7   3,012,537   -        +1.7   3,012,537   -        -0.4   3,012,806   -    Pf1N1B4_2675   0.21  +0.5   3,012,883   +    Pf1N1B4_2675   0.25  -0.7   3,012,884   -    Pf1N1B4_2675   0.25  +0.0 
 
Or see this region's nucleotide sequence