Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2554 and Pf1N1B4_2555 are separated by 82 nucleotides Pf1N1B4_2555 and Pf1N1B4_2556 are separated by 50 nucleotides Pf1N1B4_2556 and Pf1N1B4_2557 are separated by 70 nucleotides
Pf1N1B4_2554 - Bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) (EC 3.6.1.17), at 2,897,002 to 2,897,340
_2554
Pf1N1B4_2555 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,897,423 to 2,898,223
_2555
Pf1N1B4_2556 - FIG00954311: hypothetical protein, at 2,898,274 to 2,899,251
_2556
Pf1N1B4_2557 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 2,899,322 to 2,900,290
_2557
Position (kb)
2898
2899
2900 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.285 kb on + strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.286 kb on - strand, within Pf1N1B4_2554 at 2897.317 kb on - strand at 2897.317 kb on - strand at 2897.334 kb on + strand at 2897.377 kb on - strand at 2897.387 kb on + strand at 2897.436 kb on + strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.437 kb on - strand at 2897.533 kb on + strand, within Pf1N1B4_2555 at 2897.533 kb on + strand at 2897.534 kb on - strand, within Pf1N1B4_2555 at 2897.534 kb on - strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.568 kb on + strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.569 kb on - strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.739 kb on + strand, within Pf1N1B4_2555 at 2897.740 kb on - strand, within Pf1N1B4_2555 at 2897.740 kb on - strand, within Pf1N1B4_2555 at 2897.755 kb on - strand at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.757 kb on + strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2897.758 kb on - strand, within Pf1N1B4_2555 at 2898.292 kb on + strand at 2898.292 kb on + strand at 2898.293 kb on - strand at 2898.293 kb on - strand at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.401 kb on + strand, within Pf1N1B4_2556 at 2898.402 kb on - strand, within Pf1N1B4_2556 at 2898.402 kb on - strand, within Pf1N1B4_2556 at 2898.402 kb on - strand, within Pf1N1B4_2556 at 2898.402 kb on - strand, within Pf1N1B4_2556 at 2898.525 kb on + strand, within Pf1N1B4_2556 at 2898.583 kb on + strand, within Pf1N1B4_2556 at 2898.584 kb on - strand, within Pf1N1B4_2556 at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.586 kb on + strand at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.586 kb on + strand, within Pf1N1B4_2556 at 2898.587 kb on - strand, within Pf1N1B4_2556 at 2898.587 kb on - strand, within Pf1N1B4_2556 at 2898.762 kb on + strand, within Pf1N1B4_2556 at 2898.935 kb on + strand, within Pf1N1B4_2556 at 2898.935 kb on + strand, within Pf1N1B4_2556 at 2898.935 kb on + strand, within Pf1N1B4_2556 at 2898.936 kb on - strand, within Pf1N1B4_2556 at 2898.936 kb on - strand, within Pf1N1B4_2556 at 2899.030 kb on + strand, within Pf1N1B4_2556 at 2899.030 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.070 kb on + strand, within Pf1N1B4_2556 at 2899.071 kb on - strand at 2899.071 kb on - strand, within Pf1N1B4_2556 at 2899.071 kb on - strand, within Pf1N1B4_2556 at 2899.071 kb on - strand, within Pf1N1B4_2556 at 2899.282 kb on - strand at 2899.282 kb on - strand at 2899.284 kb on + strand at 2899.284 kb on + strand at 2899.284 kb on + strand at 2899.295 kb on - strand at 2899.309 kb on + strand at 2899.309 kb on + strand at 2899.309 kb on + strand at 2899.309 kb on + strand at 2899.310 kb on - strand at 2899.587 kb on - strand, within Pf1N1B4_2557 at 2899.607 kb on - strand, within Pf1N1B4_2557 at 2899.648 kb on + strand, within Pf1N1B4_2557 at 2899.649 kb on - strand, within Pf1N1B4_2557 at 2899.649 kb on - strand, within Pf1N1B4_2557 at 2899.650 kb on + strand, within Pf1N1B4_2557 at 2899.650 kb on + strand, within Pf1N1B4_2557 at 2899.650 kb on + strand, within Pf1N1B4_2557 at 2899.650 kb on + strand, within Pf1N1B4_2557 at 2899.651 kb on - strand, within Pf1N1B4_2557 at 2899.651 kb on - strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2900.000 kb on + strand, within Pf1N1B4_2557 at 2900.076 kb on + strand, within Pf1N1B4_2557 at 2900.076 kb on + strand at 2900.180 kb on + strand, within Pf1N1B4_2557 at 2900.224 kb on + strand at 2900.225 kb on - strand at 2900.243 kb on + strand at 2900.244 kb on - strand at 2900.244 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 2,897,285 + Pf1N1B4_2554 0.83 -0.7 2,897,285 + Pf1N1B4_2554 0.83 -3.2 2,897,285 + Pf1N1B4_2554 0.83 +1.3 2,897,285 + Pf1N1B4_2554 0.83 -0.3 2,897,285 + Pf1N1B4_2554 0.83 -0.3 2,897,285 + Pf1N1B4_2554 0.83 -0.0 2,897,285 + Pf1N1B4_2554 0.83 -2.0 2,897,286 - Pf1N1B4_2554 0.84 -0.8 2,897,286 - Pf1N1B4_2554 0.84 +0.3 2,897,286 - Pf1N1B4_2554 0.84 -0.7 2,897,286 - Pf1N1B4_2554 0.84 -2.5 2,897,317 - -0.4 2,897,317 - -0.0 2,897,334 + -1.3 2,897,377 - +0.4 2,897,387 + +2.1 2,897,436 + +1.8 2,897,437 - +0.5 2,897,437 - +1.0 2,897,437 - +1.3 2,897,437 - -0.6 2,897,533 + Pf1N1B4_2555 0.14 +1.1 2,897,533 + +1.3 2,897,534 - Pf1N1B4_2555 0.14 +0.3 2,897,534 - Pf1N1B4_2555 0.14 -1.3 2,897,568 + Pf1N1B4_2555 0.18 +0.3 2,897,568 + Pf1N1B4_2555 0.18 +1.0 2,897,568 + Pf1N1B4_2555 0.18 -0.7 2,897,568 + Pf1N1B4_2555 0.18 -1.0 2,897,568 + Pf1N1B4_2555 0.18 -0.1 2,897,569 - Pf1N1B4_2555 0.18 -1.9 2,897,569 - Pf1N1B4_2555 0.18 +1.3 2,897,569 - Pf1N1B4_2555 0.18 -0.7 2,897,569 - Pf1N1B4_2555 0.18 -0.4 2,897,569 - Pf1N1B4_2555 0.18 -0.3 2,897,739 + Pf1N1B4_2555 0.39 -0.6 2,897,739 + Pf1N1B4_2555 0.39 -1.5 2,897,739 + Pf1N1B4_2555 0.39 -0.3 2,897,739 + Pf1N1B4_2555 0.39 -1.9 2,897,740 - Pf1N1B4_2555 0.40 +0.6 2,897,740 - Pf1N1B4_2555 0.40 -1.0 2,897,755 - +1.3 2,897,757 + Pf1N1B4_2555 0.42 +0.3 2,897,757 + Pf1N1B4_2555 0.42 -0.7 2,897,757 + Pf1N1B4_2555 0.42 -0.9 2,897,757 + Pf1N1B4_2555 0.42 -0.4 2,897,757 + Pf1N1B4_2555 0.42 -0.9 2,897,757 + Pf1N1B4_2555 0.42 -0.9 2,897,758 - Pf1N1B4_2555 0.42 -1.5 2,897,758 - Pf1N1B4_2555 0.42 -1.5 2,897,758 - Pf1N1B4_2555 0.42 +0.8 2,897,758 - Pf1N1B4_2555 0.42 +0.4 2,897,758 - Pf1N1B4_2555 0.42 -1.3 2,897,758 - Pf1N1B4_2555 0.42 +0.3 2,897,758 - Pf1N1B4_2555 0.42 +0.4 2,897,758 - Pf1N1B4_2555 0.42 -0.0 2,897,758 - Pf1N1B4_2555 0.42 +0.7 2,897,758 - Pf1N1B4_2555 0.42 -0.5 2,897,758 - Pf1N1B4_2555 0.42 -0.8 2,898,292 + +0.3 2,898,292 + -0.3 2,898,293 - -0.3 2,898,293 - -3.2 2,898,401 + Pf1N1B4_2556 0.13 -0.5 2,898,401 + Pf1N1B4_2556 0.13 +1.0 2,898,401 + Pf1N1B4_2556 0.13 +0.3 2,898,401 + Pf1N1B4_2556 0.13 +0.6 2,898,401 + Pf1N1B4_2556 0.13 +1.7 2,898,401 + Pf1N1B4_2556 0.13 -1.0 2,898,401 + Pf1N1B4_2556 0.13 -0.0 2,898,402 - Pf1N1B4_2556 0.13 -0.4 2,898,402 - Pf1N1B4_2556 0.13 -0.3 2,898,402 - Pf1N1B4_2556 0.13 -0.3 2,898,402 - Pf1N1B4_2556 0.13 -0.3 2,898,525 + Pf1N1B4_2556 0.26 +0.8 2,898,583 + Pf1N1B4_2556 0.32 +1.0 2,898,584 - Pf1N1B4_2556 0.32 -1.5 2,898,586 + Pf1N1B4_2556 0.32 -1.6 2,898,586 + Pf1N1B4_2556 0.32 +0.1 2,898,586 + +1.3 2,898,586 + Pf1N1B4_2556 0.32 +0.3 2,898,586 + Pf1N1B4_2556 0.32 -0.1 2,898,586 + Pf1N1B4_2556 0.32 +0.6 2,898,587 - Pf1N1B4_2556 0.32 -0.0 2,898,587 - Pf1N1B4_2556 0.32 -0.5 2,898,762 + Pf1N1B4_2556 0.50 -1.3 2,898,935 + Pf1N1B4_2556 0.68 +1.8 2,898,935 + Pf1N1B4_2556 0.68 +0.8 2,898,935 + Pf1N1B4_2556 0.68 +0.8 2,898,936 - Pf1N1B4_2556 0.68 -0.6 2,898,936 - Pf1N1B4_2556 0.68 +2.1 2,899,030 + Pf1N1B4_2556 0.77 -0.4 2,899,030 + Pf1N1B4_2556 0.77 -0.2 2,899,070 + Pf1N1B4_2556 0.81 +0.9 2,899,070 + Pf1N1B4_2556 0.81 -1.0 2,899,070 + Pf1N1B4_2556 0.81 -1.7 2,899,070 + Pf1N1B4_2556 0.81 -2.3 2,899,070 + Pf1N1B4_2556 0.81 +1.5 2,899,070 + Pf1N1B4_2556 0.81 -0.4 2,899,071 - +0.3 2,899,071 - Pf1N1B4_2556 0.81 -0.6 2,899,071 - Pf1N1B4_2556 0.81 -1.7 2,899,071 - Pf1N1B4_2556 0.81 +1.0 2,899,282 - -1.0 2,899,282 - -2.3 2,899,284 + +0.9 2,899,284 + +0.5 2,899,284 + -1.5 2,899,295 - +1.0 2,899,309 + -1.7 2,899,309 + +0.3 2,899,309 + +1.7 2,899,309 + -1.9 2,899,310 - -0.5 2,899,587 - Pf1N1B4_2557 0.27 -1.5 2,899,607 - Pf1N1B4_2557 0.29 +0.6 2,899,648 + Pf1N1B4_2557 0.34 -0.4 2,899,649 - Pf1N1B4_2557 0.34 +0.3 2,899,649 - Pf1N1B4_2557 0.34 -1.7 2,899,650 + Pf1N1B4_2557 0.34 +0.8 2,899,650 + Pf1N1B4_2557 0.34 -0.6 2,899,650 + Pf1N1B4_2557 0.34 +0.3 2,899,650 + Pf1N1B4_2557 0.34 -1.2 2,899,651 - Pf1N1B4_2557 0.34 -1.1 2,899,651 - Pf1N1B4_2557 0.34 +0.1 2,899,941 + Pf1N1B4_2557 0.64 -0.9 2,899,941 + Pf1N1B4_2557 0.64 -0.3 2,899,941 + Pf1N1B4_2557 0.64 -1.9 2,900,000 + Pf1N1B4_2557 0.70 +0.1 2,900,076 + Pf1N1B4_2557 0.78 +0.1 2,900,076 + -0.3 2,900,180 + Pf1N1B4_2557 0.89 -0.8 2,900,224 + +0.7 2,900,225 - +2.9 2,900,243 + +0.8 2,900,244 - +0.6 2,900,244 - -1.3
Or see this region's nucleotide sequence