Experiment: Spectinomycin 0.05 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_2538 and Pf1N1B4_2539 are separated by 49 nucleotides Pf1N1B4_2539 and Pf1N1B4_2540 are separated by 198 nucleotides  
        
        Pf1N1B4_2538 - Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1), at 2,879,841 to 2,880,965 
         
        _2538 
         
        
        Pf1N1B4_2539 - ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1), at 2,881,015 to 2,882,058 
         
        _2539 
         
        
        Pf1N1B4_2540 - Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1), at 2,882,257 to 2,883,504 
         
        _2540 
         Position (kb)  
2881 
 
2882 
 
2883 Strain fitness (log2 ratio)  
-3 
 
-2 
 
-1 
 
0 
 
1 
 
2 
 
3 at 2880.126 kb on + strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.127 kb on - strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.441 kb on + strand, within Pf1N1B4_2538 at 2880.442 kb on - strand at 2880.540 kb on + strand, within Pf1N1B4_2538 at 2880.540 kb on + strand, within Pf1N1B4_2538 at 2880.541 kb on - strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.795 kb on + strand, within Pf1N1B4_2538 at 2880.796 kb on - strand at 2880.952 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.012 kb on + strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.013 kb on - strand at 2881.147 kb on + strand, within Pf1N1B4_2539 at 2881.148 kb on - strand, within Pf1N1B4_2539 at 2881.148 kb on - strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.195 kb on + strand, within Pf1N1B4_2539 at 2881.259 kb on + strand, within Pf1N1B4_2539 at 2881.260 kb on - strand at 2881.348 kb on + strand, within Pf1N1B4_2539 at 2881.349 kb on - strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.510 kb on + strand, within Pf1N1B4_2539 at 2881.511 kb on - strand, within Pf1N1B4_2539 at 2881.561 kb on + strand, within Pf1N1B4_2539 at 2881.561 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.568 kb on + strand, within Pf1N1B4_2539 at 2881.666 kb on + strand, within Pf1N1B4_2539 at 2881.702 kb on + strand, within Pf1N1B4_2539 at 2881.768 kb on + strand, within Pf1N1B4_2539 at 2881.769 kb on - strand, within Pf1N1B4_2539 at 2881.873 kb on + strand, within Pf1N1B4_2539 at 2881.874 kb on - strand, within Pf1N1B4_2539 at 2882.057 kb on - strand at 2882.065 kb on + strand at 2882.065 kb on + strand at 2882.251 kb on + strand at 2882.251 kb on + strand at 2882.252 kb on - strand at 2882.252 kb on - strand at 2882.252 kb on - strand at 2882.252 kb on - strand at 2882.417 kb on - strand, within Pf1N1B4_2540 at 2882.417 kb on - strand, within Pf1N1B4_2540 at 2882.417 kb on - strand, within Pf1N1B4_2540 at 2882.417 kb on - strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.515 kb on + strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.516 kb on - strand, within Pf1N1B4_2540 at 2882.517 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.665 kb on + strand, within Pf1N1B4_2540 at 2882.666 kb on - strand, within Pf1N1B4_2540 at 2882.666 kb on - strand, within Pf1N1B4_2540 at 2882.666 kb on - strand, within Pf1N1B4_2540 at 2882.666 kb on - strand at 2882.668 kb on - strand, within Pf1N1B4_2540 at 2882.668 kb on - strand, within Pf1N1B4_2540 at 2882.713 kb on + strand, within Pf1N1B4_2540 at 2882.742 kb on + strand at 2882.742 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.755 kb on + strand, within Pf1N1B4_2540 at 2882.756 kb on - strand, within Pf1N1B4_2540 at 2882.758 kb on + strand, within Pf1N1B4_2540 at 2882.759 kb on - strand, within Pf1N1B4_2540 at 2882.833 kb on + strand, within Pf1N1B4_2540 at 2882.833 kb on + strand, within Pf1N1B4_2540 at 2882.833 kb on + strand, within Pf1N1B4_2540 at 2882.834 kb on - strand, within Pf1N1B4_2540 at 2882.915 kb on - strand, within Pf1N1B4_2540  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Spectinomycin 0.05 mg/ml             remove   2,880,126   +    Pf1N1B4_2538   0.25  +1.3   2,880,127   -    Pf1N1B4_2538   0.25  -2.9   2,880,127   -    Pf1N1B4_2538   0.25  +0.6   2,880,127   -    Pf1N1B4_2538   0.25  +0.0   2,880,127   -    Pf1N1B4_2538   0.25  +0.9   2,880,127   -    Pf1N1B4_2538   0.25  -1.8   2,880,441   +    Pf1N1B4_2538   0.53  -0.7   2,880,441   +    Pf1N1B4_2538   0.53  +1.5   2,880,441   +    Pf1N1B4_2538   0.53  -0.1   2,880,442   -        +0.3   2,880,540   +    Pf1N1B4_2538   0.62  +0.6   2,880,540   +    Pf1N1B4_2538   0.62  -1.0   2,880,541   -    Pf1N1B4_2538   0.62  +0.9   2,880,795   +    Pf1N1B4_2538   0.85  -0.5   2,880,795   +    Pf1N1B4_2538   0.85  +0.0   2,880,795   +    Pf1N1B4_2538   0.85  -1.9   2,880,795   +    Pf1N1B4_2538   0.85  -0.2   2,880,795   +    Pf1N1B4_2538   0.85  -1.7   2,880,795   +    Pf1N1B4_2538   0.85  -1.0   2,880,795   +    Pf1N1B4_2538   0.85  +0.1   2,880,796   -        +2.9   2,880,952   +        -0.7   2,881,012   +        -0.3   2,881,012   +        +0.3   2,881,012   +        +0.5   2,881,012   +        +0.1   2,881,012   +        -1.1   2,881,012   +        -0.2   2,881,012   +        -0.7   2,881,012   +        +0.7   2,881,012   +        -2.4   2,881,013   -        +0.8   2,881,013   -        +0.1   2,881,013   -        -0.4   2,881,013   -        -0.5   2,881,013   -        +0.3   2,881,013   -        +0.5   2,881,013   -        +0.5   2,881,147   +    Pf1N1B4_2539   0.13  -0.9   2,881,148   -    Pf1N1B4_2539   0.13  +0.1   2,881,148   -    Pf1N1B4_2539   0.13  +1.0   2,881,195   +    Pf1N1B4_2539   0.17  -2.5   2,881,195   +    Pf1N1B4_2539   0.17  -0.1   2,881,195   +    Pf1N1B4_2539   0.17  -0.2   2,881,259   +    Pf1N1B4_2539   0.23  +0.3   2,881,260   -        +1.3   2,881,348   +    Pf1N1B4_2539   0.32  -1.3   2,881,349   -    Pf1N1B4_2539   0.32  +0.6   2,881,510   +    Pf1N1B4_2539   0.47  -0.7   2,881,510   +    Pf1N1B4_2539   0.47  +1.1   2,881,510   +    Pf1N1B4_2539   0.47  -0.7   2,881,511   -    Pf1N1B4_2539   0.48  -0.0   2,881,561   +    Pf1N1B4_2539   0.52  -1.5   2,881,561   +    Pf1N1B4_2539   0.52  -1.7   2,881,568   +    Pf1N1B4_2539   0.53  -0.6   2,881,568   +    Pf1N1B4_2539   0.53  +0.5   2,881,568   +    Pf1N1B4_2539   0.53  -0.1   2,881,666   +    Pf1N1B4_2539   0.62  +0.5   2,881,702   +    Pf1N1B4_2539   0.66  -2.2   2,881,768   +    Pf1N1B4_2539   0.72  -0.7   2,881,769   -    Pf1N1B4_2539   0.72  -0.0   2,881,873   +    Pf1N1B4_2539   0.82  -0.9   2,881,874   -    Pf1N1B4_2539   0.82  -0.7   2,882,057   -        -1.0   2,882,065   +        -0.3   2,882,065   +        -0.4   2,882,251   +        -1.2   2,882,251   +        -0.0   2,882,252   -        +0.3   2,882,252   -        -0.7   2,882,252   -        -0.4   2,882,252   -        +1.0   2,882,417   -    Pf1N1B4_2540   0.13  -2.3   2,882,417   -    Pf1N1B4_2540   0.13  -1.1   2,882,417   -    Pf1N1B4_2540   0.13  +0.1   2,882,417   -    Pf1N1B4_2540   0.13  -0.9   2,882,515   +    Pf1N1B4_2540   0.21  +0.7   2,882,515   +    Pf1N1B4_2540   0.21  -1.0   2,882,515   +    Pf1N1B4_2540   0.21  +0.7   2,882,515   +    Pf1N1B4_2540   0.21  +2.0   2,882,515   +    Pf1N1B4_2540   0.21  +1.9   2,882,515   +    Pf1N1B4_2540   0.21  -0.3   2,882,515   +    Pf1N1B4_2540   0.21  -1.5   2,882,515   +    Pf1N1B4_2540   0.21  +0.8   2,882,515   +    Pf1N1B4_2540   0.21  -0.3   2,882,515   +    Pf1N1B4_2540   0.21  -0.2   2,882,516   -    Pf1N1B4_2540   0.21  -1.3   2,882,516   -    Pf1N1B4_2540   0.21  -0.7   2,882,516   -    Pf1N1B4_2540   0.21  +0.8   2,882,516   -    Pf1N1B4_2540   0.21  -0.0   2,882,516   -    Pf1N1B4_2540   0.21  -1.2   2,882,516   -    Pf1N1B4_2540   0.21  +0.4   2,882,517   +    Pf1N1B4_2540   0.21  +1.1   2,882,665   +    Pf1N1B4_2540   0.33  +1.0   2,882,665   +    Pf1N1B4_2540   0.33  -0.4   2,882,665   +    Pf1N1B4_2540   0.33  +0.2   2,882,665   +    Pf1N1B4_2540   0.33  -0.6   2,882,665   +    Pf1N1B4_2540   0.33  -1.2   2,882,665   +    Pf1N1B4_2540   0.33  +0.3   2,882,665   +    Pf1N1B4_2540   0.33  -0.3   2,882,665   +    Pf1N1B4_2540   0.33  +0.4   2,882,665   +    Pf1N1B4_2540   0.33  -0.2   2,882,665   +    Pf1N1B4_2540   0.33  -0.4   2,882,665   +    Pf1N1B4_2540   0.33  +0.4   2,882,665   +    Pf1N1B4_2540   0.33  -0.3   2,882,665   +    Pf1N1B4_2540   0.33  +0.1   2,882,666   -    Pf1N1B4_2540   0.33  +0.2   2,882,666   -    Pf1N1B4_2540   0.33  +0.6   2,882,666   -    Pf1N1B4_2540   0.33  +0.3   2,882,666   -        -0.7   2,882,668   -    Pf1N1B4_2540   0.33  -1.9   2,882,668   -    Pf1N1B4_2540   0.33  -2.2   2,882,713   +    Pf1N1B4_2540   0.37  +0.3   2,882,742   +        +0.7   2,882,742   +    Pf1N1B4_2540   0.39  -0.3   2,882,755   +    Pf1N1B4_2540   0.40  -0.3   2,882,755   +    Pf1N1B4_2540   0.40  +0.9   2,882,755   +    Pf1N1B4_2540   0.40  -1.3   2,882,755   +    Pf1N1B4_2540   0.40  -0.3   2,882,755   +    Pf1N1B4_2540   0.40  -0.3   2,882,756   -    Pf1N1B4_2540   0.40  +0.1   2,882,758   +    Pf1N1B4_2540   0.40  -0.7   2,882,759   -    Pf1N1B4_2540   0.40  +1.1   2,882,833   +    Pf1N1B4_2540   0.46  +0.1   2,882,833   +    Pf1N1B4_2540   0.46  +0.5   2,882,833   +    Pf1N1B4_2540   0.46  -0.6   2,882,834   -    Pf1N1B4_2540   0.46  -0.0   2,882,915   -    Pf1N1B4_2540   0.53  -0.5 
 
Or see this region's nucleotide sequence