Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2403 and Pf1N1B4_2404 are separated by 580 nucleotides Pf1N1B4_2404 and Pf1N1B4_2405 are separated by 333 nucleotides
Pf1N1B4_2403 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 2,721,915 to 2,722,277
_2403
Pf1N1B4_2404 - Alginate lyase precursor (EC 4.2.2.3), at 2,722,858 to 2,723,526
_2404
Pf1N1B4_2405 - Sensory box/GGDEF family protein, at 2,723,860 to 2,725,965
_2405
Position (kb)
2722
2723
2724 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2721.916 kb on + strand at 2721.916 kb on + strand at 2721.916 kb on + strand at 2722.099 kb on + strand at 2722.099 kb on + strand, within Pf1N1B4_2403 at 2722.100 kb on - strand, within Pf1N1B4_2403 at 2722.100 kb on - strand, within Pf1N1B4_2403 at 2722.100 kb on - strand, within Pf1N1B4_2403 at 2722.117 kb on + strand, within Pf1N1B4_2403 at 2722.117 kb on + strand, within Pf1N1B4_2403 at 2722.117 kb on + strand, within Pf1N1B4_2403 at 2722.117 kb on + strand, within Pf1N1B4_2403 at 2722.117 kb on + strand, within Pf1N1B4_2403 at 2722.118 kb on - strand at 2722.118 kb on - strand, within Pf1N1B4_2403 at 2722.118 kb on - strand, within Pf1N1B4_2403 at 2722.118 kb on - strand, within Pf1N1B4_2403 at 2722.118 kb on - strand, within Pf1N1B4_2403 at 2722.118 kb on - strand, within Pf1N1B4_2403 at 2722.118 kb on - strand, within Pf1N1B4_2403 at 2722.118 kb on - strand, within Pf1N1B4_2403 at 2722.118 kb on - strand, within Pf1N1B4_2403 at 2722.171 kb on + strand, within Pf1N1B4_2403 at 2722.172 kb on - strand, within Pf1N1B4_2403 at 2722.172 kb on - strand, within Pf1N1B4_2403 at 2722.172 kb on - strand, within Pf1N1B4_2403 at 2722.172 kb on - strand, within Pf1N1B4_2403 at 2722.172 kb on - strand, within Pf1N1B4_2403 at 2722.271 kb on + strand at 2722.272 kb on - strand at 2722.272 kb on - strand at 2722.317 kb on + strand at 2722.318 kb on - strand at 2722.318 kb on - strand at 2722.338 kb on + strand at 2722.339 kb on - strand at 2722.350 kb on + strand at 2722.351 kb on - strand at 2722.402 kb on + strand at 2722.402 kb on + strand at 2722.402 kb on + strand at 2722.402 kb on + strand at 2722.402 kb on + strand at 2722.402 kb on + strand at 2722.402 kb on + strand at 2722.402 kb on + strand at 2722.402 kb on + strand at 2722.403 kb on - strand at 2722.403 kb on - strand at 2722.471 kb on + strand at 2722.474 kb on + strand at 2722.474 kb on + strand at 2722.474 kb on + strand at 2722.474 kb on + strand at 2722.554 kb on + strand at 2722.583 kb on - strand at 2722.583 kb on - strand at 2722.583 kb on - strand at 2722.597 kb on - strand at 2722.612 kb on + strand at 2722.626 kb on + strand at 2722.626 kb on + strand at 2722.626 kb on + strand at 2722.627 kb on - strand at 2722.671 kb on - strand at 2722.671 kb on - strand at 2722.671 kb on - strand at 2722.671 kb on - strand at 2722.671 kb on - strand at 2722.789 kb on - strand at 2722.789 kb on - strand at 2722.840 kb on - strand at 2722.914 kb on + strand at 2722.915 kb on - strand at 2722.915 kb on - strand at 2722.915 kb on - strand at 2722.937 kb on + strand, within Pf1N1B4_2404 at 2722.937 kb on + strand, within Pf1N1B4_2404 at 2722.938 kb on - strand, within Pf1N1B4_2404 at 2722.938 kb on - strand, within Pf1N1B4_2404 at 2722.938 kb on - strand, within Pf1N1B4_2404 at 2722.955 kb on + strand, within Pf1N1B4_2404 at 2722.955 kb on + strand, within Pf1N1B4_2404 at 2722.956 kb on - strand, within Pf1N1B4_2404 at 2722.956 kb on - strand, within Pf1N1B4_2404 at 2722.965 kb on + strand, within Pf1N1B4_2404 at 2723.077 kb on + strand, within Pf1N1B4_2404 at 2723.078 kb on - strand, within Pf1N1B4_2404 at 2723.163 kb on - strand, within Pf1N1B4_2404 at 2723.169 kb on + strand, within Pf1N1B4_2404 at 2723.169 kb on + strand, within Pf1N1B4_2404 at 2723.169 kb on + strand, within Pf1N1B4_2404 at 2723.169 kb on + strand, within Pf1N1B4_2404 at 2723.169 kb on + strand, within Pf1N1B4_2404 at 2723.169 kb on + strand, within Pf1N1B4_2404 at 2723.170 kb on - strand, within Pf1N1B4_2404 at 2723.171 kb on + strand, within Pf1N1B4_2404 at 2723.171 kb on + strand, within Pf1N1B4_2404 at 2723.171 kb on + strand, within Pf1N1B4_2404 at 2723.171 kb on + strand, within Pf1N1B4_2404 at 2723.172 kb on - strand, within Pf1N1B4_2404 at 2723.188 kb on + strand, within Pf1N1B4_2404 at 2723.188 kb on + strand, within Pf1N1B4_2404 at 2723.210 kb on + strand, within Pf1N1B4_2404 at 2723.211 kb on - strand, within Pf1N1B4_2404 at 2723.252 kb on - strand, within Pf1N1B4_2404 at 2723.303 kb on + strand, within Pf1N1B4_2404 at 2723.303 kb on + strand, within Pf1N1B4_2404 at 2723.304 kb on - strand, within Pf1N1B4_2404 at 2723.358 kb on - strand, within Pf1N1B4_2404 at 2723.429 kb on + strand, within Pf1N1B4_2404 at 2723.429 kb on + strand, within Pf1N1B4_2404 at 2723.430 kb on - strand, within Pf1N1B4_2404 at 2723.430 kb on - strand, within Pf1N1B4_2404 at 2723.430 kb on - strand, within Pf1N1B4_2404 at 2723.430 kb on - strand, within Pf1N1B4_2404 at 2723.430 kb on - strand, within Pf1N1B4_2404 at 2723.430 kb on - strand, within Pf1N1B4_2404 at 2723.475 kb on - strand at 2723.475 kb on - strand at 2723.527 kb on - strand at 2723.527 kb on - strand at 2723.550 kb on + strand at 2723.550 kb on + strand at 2723.594 kb on + strand at 2723.594 kb on + strand at 2723.594 kb on + strand at 2723.594 kb on + strand at 2723.595 kb on - strand at 2723.595 kb on - strand at 2723.595 kb on - strand at 2723.595 kb on - strand at 2723.595 kb on - strand at 2723.596 kb on + strand at 2723.597 kb on - strand at 2723.597 kb on - strand at 2723.602 kb on + strand at 2723.602 kb on + strand at 2723.603 kb on - strand at 2723.603 kb on - strand at 2723.691 kb on - strand at 2723.703 kb on + strand at 2723.713 kb on + strand at 2723.714 kb on - strand at 2723.722 kb on + strand at 2723.722 kb on + strand at 2723.772 kb on - strand at 2723.787 kb on + strand at 2723.787 kb on + strand at 2723.787 kb on + strand at 2723.787 kb on + strand at 2723.787 kb on + strand at 2723.787 kb on + strand at 2723.787 kb on + strand at 2723.787 kb on + strand at 2723.788 kb on - strand at 2723.788 kb on - strand at 2723.788 kb on - strand at 2723.859 kb on + strand at 2723.859 kb on + strand at 2723.859 kb on + strand at 2723.859 kb on + strand at 2723.860 kb on - strand at 2723.860 kb on - strand at 2723.860 kb on - strand at 2723.860 kb on - strand at 2723.860 kb on - strand at 2723.860 kb on - strand at 2723.860 kb on - strand at 2723.860 kb on - strand at 2723.860 kb on - strand at 2723.888 kb on + strand at 2723.889 kb on - strand at 2723.889 kb on - strand at 2723.889 kb on - strand at 2723.997 kb on - strand at 2724.020 kb on + strand at 2724.178 kb on + strand, within Pf1N1B4_2405 at 2724.178 kb on + strand, within Pf1N1B4_2405 at 2724.178 kb on + strand, within Pf1N1B4_2405 at 2724.178 kb on + strand, within Pf1N1B4_2405 at 2724.179 kb on - strand, within Pf1N1B4_2405 at 2724.179 kb on - strand, within Pf1N1B4_2405 at 2724.179 kb on - strand, within Pf1N1B4_2405 at 2724.246 kb on + strand, within Pf1N1B4_2405 at 2724.246 kb on + strand, within Pf1N1B4_2405
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 2,721,916 + +0.4 2,721,916 + -0.3 2,721,916 + -1.5 2,722,099 + -0.6 2,722,099 + Pf1N1B4_2403 0.51 -0.4 2,722,100 - Pf1N1B4_2403 0.51 -1.0 2,722,100 - Pf1N1B4_2403 0.51 -1.2 2,722,100 - Pf1N1B4_2403 0.51 -0.6 2,722,117 + Pf1N1B4_2403 0.56 +0.0 2,722,117 + Pf1N1B4_2403 0.56 +1.6 2,722,117 + Pf1N1B4_2403 0.56 +0.8 2,722,117 + Pf1N1B4_2403 0.56 -1.0 2,722,117 + Pf1N1B4_2403 0.56 +0.0 2,722,118 - -0.6 2,722,118 - Pf1N1B4_2403 0.56 +1.4 2,722,118 - Pf1N1B4_2403 0.56 +0.0 2,722,118 - Pf1N1B4_2403 0.56 +0.2 2,722,118 - Pf1N1B4_2403 0.56 +0.8 2,722,118 - Pf1N1B4_2403 0.56 +0.4 2,722,118 - Pf1N1B4_2403 0.56 +1.0 2,722,118 - Pf1N1B4_2403 0.56 +1.0 2,722,118 - Pf1N1B4_2403 0.56 +0.4 2,722,171 + Pf1N1B4_2403 0.71 -2.3 2,722,172 - Pf1N1B4_2403 0.71 -0.8 2,722,172 - Pf1N1B4_2403 0.71 -0.6 2,722,172 - Pf1N1B4_2403 0.71 -1.2 2,722,172 - Pf1N1B4_2403 0.71 -1.0 2,722,172 - Pf1N1B4_2403 0.71 +0.8 2,722,271 + -0.4 2,722,272 - -0.1 2,722,272 - +0.0 2,722,317 + -0.2 2,722,318 - +0.0 2,722,318 - +0.4 2,722,338 + -1.3 2,722,339 - -0.7 2,722,350 + +1.4 2,722,351 - -1.0 2,722,402 + -0.3 2,722,402 + -0.3 2,722,402 + -1.0 2,722,402 + -0.3 2,722,402 + -0.6 2,722,402 + -0.8 2,722,402 + +0.9 2,722,402 + +0.6 2,722,402 + -0.5 2,722,403 - -1.0 2,722,403 - -0.4 2,722,471 + +3.8 2,722,474 + +0.2 2,722,474 + -0.5 2,722,474 + +0.4 2,722,474 + +0.3 2,722,554 + +0.4 2,722,583 - +0.7 2,722,583 - -0.4 2,722,583 - +0.6 2,722,597 - -0.0 2,722,612 + -1.1 2,722,626 + -1.8 2,722,626 + -1.2 2,722,626 + +0.7 2,722,627 - +0.1 2,722,671 - -0.6 2,722,671 - +0.6 2,722,671 - -1.4 2,722,671 - -0.1 2,722,671 - +0.9 2,722,789 - +0.9 2,722,789 - +0.6 2,722,840 - -2.0 2,722,914 + -0.4 2,722,915 - -1.8 2,722,915 - +0.1 2,722,915 - +1.7 2,722,937 + Pf1N1B4_2404 0.12 +0.8 2,722,937 + Pf1N1B4_2404 0.12 +0.0 2,722,938 - Pf1N1B4_2404 0.12 +0.8 2,722,938 - Pf1N1B4_2404 0.12 +0.8 2,722,938 - Pf1N1B4_2404 0.12 -0.9 2,722,955 + Pf1N1B4_2404 0.14 +1.9 2,722,955 + Pf1N1B4_2404 0.14 +0.9 2,722,956 - Pf1N1B4_2404 0.15 +0.6 2,722,956 - Pf1N1B4_2404 0.15 +0.2 2,722,965 + Pf1N1B4_2404 0.16 -0.2 2,723,077 + Pf1N1B4_2404 0.33 +2.4 2,723,078 - Pf1N1B4_2404 0.33 +1.2 2,723,163 - Pf1N1B4_2404 0.46 -0.1 2,723,169 + Pf1N1B4_2404 0.46 -1.7 2,723,169 + Pf1N1B4_2404 0.46 -0.4 2,723,169 + Pf1N1B4_2404 0.46 -1.0 2,723,169 + Pf1N1B4_2404 0.46 +1.4 2,723,169 + Pf1N1B4_2404 0.46 -2.0 2,723,169 + Pf1N1B4_2404 0.46 -0.5 2,723,170 - Pf1N1B4_2404 0.47 +1.2 2,723,171 + Pf1N1B4_2404 0.47 +1.1 2,723,171 + Pf1N1B4_2404 0.47 -0.5 2,723,171 + Pf1N1B4_2404 0.47 -1.1 2,723,171 + Pf1N1B4_2404 0.47 +0.2 2,723,172 - Pf1N1B4_2404 0.47 -1.6 2,723,188 + Pf1N1B4_2404 0.49 -2.1 2,723,188 + Pf1N1B4_2404 0.49 +0.1 2,723,210 + Pf1N1B4_2404 0.53 -0.6 2,723,211 - Pf1N1B4_2404 0.53 +1.4 2,723,252 - Pf1N1B4_2404 0.59 -0.1 2,723,303 + Pf1N1B4_2404 0.67 +1.0 2,723,303 + Pf1N1B4_2404 0.67 +0.6 2,723,304 - Pf1N1B4_2404 0.67 +0.5 2,723,358 - Pf1N1B4_2404 0.75 +0.4 2,723,429 + Pf1N1B4_2404 0.85 +1.0 2,723,429 + Pf1N1B4_2404 0.85 +0.5 2,723,430 - Pf1N1B4_2404 0.86 -0.0 2,723,430 - Pf1N1B4_2404 0.86 +1.4 2,723,430 - Pf1N1B4_2404 0.86 +0.7 2,723,430 - Pf1N1B4_2404 0.86 +0.1 2,723,430 - Pf1N1B4_2404 0.86 -1.0 2,723,430 - Pf1N1B4_2404 0.86 -0.6 2,723,475 - +1.4 2,723,475 - +0.9 2,723,527 - +1.5 2,723,527 - +0.8 2,723,550 + +0.4 2,723,550 + -1.4 2,723,594 + +1.0 2,723,594 + -0.6 2,723,594 + -1.2 2,723,594 + +0.4 2,723,595 - -0.2 2,723,595 - -1.2 2,723,595 - +0.9 2,723,595 - -0.7 2,723,595 - +1.2 2,723,596 + -0.7 2,723,597 - +1.1 2,723,597 - -0.0 2,723,602 + +0.6 2,723,602 + +1.7 2,723,603 - -1.2 2,723,603 - -1.4 2,723,691 - +0.0 2,723,703 + +0.9 2,723,713 + +1.9 2,723,714 - -1.1 2,723,722 + -0.6 2,723,722 + +0.2 2,723,772 - +0.4 2,723,787 + +0.8 2,723,787 + -2.9 2,723,787 + +1.0 2,723,787 + -0.8 2,723,787 + -1.0 2,723,787 + -0.5 2,723,787 + +1.5 2,723,787 + -1.2 2,723,788 - +1.4 2,723,788 - -1.2 2,723,788 - -1.6 2,723,859 + +0.2 2,723,859 + +0.4 2,723,859 + -0.5 2,723,859 + +0.2 2,723,860 - -0.2 2,723,860 - -0.0 2,723,860 - -0.6 2,723,860 - +0.6 2,723,860 - -1.0 2,723,860 - +0.9 2,723,860 - +1.6 2,723,860 - -0.1 2,723,860 - +1.9 2,723,888 + +0.8 2,723,889 - -1.1 2,723,889 - +1.4 2,723,889 - +1.4 2,723,997 - +1.6 2,724,020 + -1.2 2,724,178 + Pf1N1B4_2405 0.15 +0.4 2,724,178 + Pf1N1B4_2405 0.15 -0.2 2,724,178 + Pf1N1B4_2405 0.15 +0.1 2,724,178 + Pf1N1B4_2405 0.15 -0.4 2,724,179 - Pf1N1B4_2405 0.15 +0.1 2,724,179 - Pf1N1B4_2405 0.15 +0.6 2,724,179 - Pf1N1B4_2405 0.15 -0.1 2,724,246 + Pf1N1B4_2405 0.18 -0.5 2,724,246 + Pf1N1B4_2405 0.18 -0.2
Or see this region's nucleotide sequence