Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2348 and Pf1N1B4_2349 are separated by 99 nucleotides Pf1N1B4_2349 and Pf1N1B4_2350 overlap by 1 nucleotides Pf1N1B4_2350 and Pf1N1B4_2351 are separated by 190 nucleotides
Pf1N1B4_2348 - Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR, at 2,663,441 to 2,663,944
_2348
Pf1N1B4_2349 - Putative Holliday junction resolvase YggF, at 2,664,044 to 2,664,481
_2349
Pf1N1B4_2350 - UPF0301 protein YqgE, at 2,664,481 to 2,665,053
_2350
Pf1N1B4_2351 - Ferric siderophore transport system, periplasmic binding protein TonB, at 2,665,244 to 2,666,098
_2351
Position (kb)
2664
2665
2666 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2663.580 kb on - strand, within Pf1N1B4_2348 at 2663.580 kb on - strand, within Pf1N1B4_2348 at 2663.580 kb on - strand, within Pf1N1B4_2348 at 2663.608 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.718 kb on - strand, within Pf1N1B4_2348 at 2663.854 kb on - strand, within Pf1N1B4_2348 at 2663.854 kb on - strand, within Pf1N1B4_2348 at 2663.886 kb on + strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2663.887 kb on - strand, within Pf1N1B4_2348 at 2664.001 kb on - strand at 2664.001 kb on - strand at 2664.001 kb on - strand at 2664.005 kb on - strand at 2664.005 kb on - strand at 2664.035 kb on + strand at 2664.035 kb on + strand at 2664.035 kb on + strand at 2664.035 kb on + strand at 2664.035 kb on + strand at 2664.036 kb on - strand at 2664.036 kb on - strand at 2664.554 kb on - strand, within Pf1N1B4_2350 at 2664.554 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2664.963 kb on - strand, within Pf1N1B4_2350 at 2665.061 kb on - strand at 2665.172 kb on + strand at 2665.172 kb on + strand at 2665.172 kb on + strand at 2665.173 kb on - strand at 2665.173 kb on - strand at 2665.249 kb on + strand at 2665.249 kb on + strand at 2665.250 kb on - strand at 2665.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 2,663,580 - Pf1N1B4_2348 0.28 +0.5 2,663,580 - Pf1N1B4_2348 0.28 +0.4 2,663,580 - Pf1N1B4_2348 0.28 -1.2 2,663,608 - Pf1N1B4_2348 0.33 +0.6 2,663,718 - Pf1N1B4_2348 0.55 +0.5 2,663,718 - Pf1N1B4_2348 0.55 +0.0 2,663,718 - Pf1N1B4_2348 0.55 -0.6 2,663,718 - Pf1N1B4_2348 0.55 +0.6 2,663,718 - Pf1N1B4_2348 0.55 -0.2 2,663,718 - Pf1N1B4_2348 0.55 -0.6 2,663,718 - Pf1N1B4_2348 0.55 -1.0 2,663,718 - Pf1N1B4_2348 0.55 +0.8 2,663,718 - Pf1N1B4_2348 0.55 -0.5 2,663,854 - Pf1N1B4_2348 0.82 -0.5 2,663,854 - Pf1N1B4_2348 0.82 -2.3 2,663,886 + Pf1N1B4_2348 0.88 -1.8 2,663,887 - Pf1N1B4_2348 0.88 +0.4 2,663,887 - Pf1N1B4_2348 0.88 -0.3 2,663,887 - Pf1N1B4_2348 0.88 +1.6 2,663,887 - Pf1N1B4_2348 0.88 -1.0 2,663,887 - Pf1N1B4_2348 0.88 -1.4 2,663,887 - Pf1N1B4_2348 0.88 -1.8 2,663,887 - Pf1N1B4_2348 0.88 -0.9 2,663,887 - Pf1N1B4_2348 0.88 -1.2 2,663,887 - Pf1N1B4_2348 0.88 -0.6 2,663,887 - Pf1N1B4_2348 0.88 -0.3 2,663,887 - Pf1N1B4_2348 0.88 +0.6 2,663,887 - Pf1N1B4_2348 0.88 +0.1 2,664,001 - -0.2 2,664,001 - -0.6 2,664,001 - +0.1 2,664,005 - +0.5 2,664,005 - -0.4 2,664,035 + +0.6 2,664,035 + +0.8 2,664,035 + -1.6 2,664,035 + -1.6 2,664,035 + -0.8 2,664,036 - -1.0 2,664,036 - -0.6 2,664,554 - Pf1N1B4_2350 0.13 +1.0 2,664,554 - Pf1N1B4_2350 0.13 -0.6 2,664,963 - Pf1N1B4_2350 0.84 -0.3 2,664,963 - Pf1N1B4_2350 0.84 -0.2 2,664,963 - Pf1N1B4_2350 0.84 -0.4 2,664,963 - Pf1N1B4_2350 0.84 -0.8 2,664,963 - Pf1N1B4_2350 0.84 -0.2 2,664,963 - Pf1N1B4_2350 0.84 -1.4 2,664,963 - Pf1N1B4_2350 0.84 +0.6 2,664,963 - Pf1N1B4_2350 0.84 -1.9 2,665,061 - +0.1 2,665,172 + +2.4 2,665,172 + +0.4 2,665,172 + +1.6 2,665,173 - +1.1 2,665,173 - -0.2 2,665,249 + +0.4 2,665,249 + +1.6 2,665,250 - -1.3 2,665,250 - +0.6
Or see this region's nucleotide sequence