Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2261 and Pf1N1B4_2262 overlap by 4 nucleotides Pf1N1B4_2262 and Pf1N1B4_2263 overlap by 4 nucleotides
Pf1N1B4_2261 - Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2), at 2,577,454 to 2,578,596
_2261
Pf1N1B4_2262 - Lysine ABC transporter, permease component (from data) , at 2,578,593 to 2,579,513
_2262
Pf1N1B4_2263 - Putrescine transport system permease protein PotI (TC 3.A.1.11.2), at 2,579,510 to 2,580,400
_2263
Position (kb)
2578
2579
2580 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2577.691 kb on + strand, within Pf1N1B4_2261 at 2577.691 kb on + strand, within Pf1N1B4_2261 at 2577.760 kb on + strand, within Pf1N1B4_2261 at 2577.887 kb on - strand, within Pf1N1B4_2261 at 2578.174 kb on + strand, within Pf1N1B4_2261 at 2578.174 kb on + strand, within Pf1N1B4_2261 at 2578.174 kb on + strand, within Pf1N1B4_2261 at 2578.174 kb on + strand, within Pf1N1B4_2261 at 2578.174 kb on + strand, within Pf1N1B4_2261 at 2578.300 kb on + strand, within Pf1N1B4_2261 at 2578.300 kb on + strand, within Pf1N1B4_2261 at 2578.300 kb on + strand, within Pf1N1B4_2261 at 2578.300 kb on + strand, within Pf1N1B4_2261 at 2578.300 kb on + strand, within Pf1N1B4_2261 at 2578.300 kb on + strand, within Pf1N1B4_2261 at 2578.300 kb on + strand, within Pf1N1B4_2261 at 2578.301 kb on - strand, within Pf1N1B4_2261 at 2578.301 kb on - strand, within Pf1N1B4_2261 at 2578.301 kb on - strand, within Pf1N1B4_2261 at 2578.348 kb on + strand, within Pf1N1B4_2261 at 2578.348 kb on + strand, within Pf1N1B4_2261 at 2578.349 kb on - strand, within Pf1N1B4_2261 at 2578.349 kb on - strand, within Pf1N1B4_2261 at 2578.349 kb on - strand, within Pf1N1B4_2261 at 2578.438 kb on + strand, within Pf1N1B4_2261 at 2578.438 kb on + strand, within Pf1N1B4_2261 at 2578.438 kb on + strand, within Pf1N1B4_2261 at 2578.462 kb on + strand, within Pf1N1B4_2261 at 2578.462 kb on + strand, within Pf1N1B4_2261 at 2578.462 kb on + strand, within Pf1N1B4_2261 at 2578.462 kb on + strand, within Pf1N1B4_2261 at 2578.463 kb on - strand, within Pf1N1B4_2261 at 2578.463 kb on - strand, within Pf1N1B4_2261 at 2578.463 kb on - strand, within Pf1N1B4_2261 at 2578.463 kb on - strand, within Pf1N1B4_2261 at 2578.463 kb on - strand, within Pf1N1B4_2261 at 2578.552 kb on + strand at 2578.552 kb on + strand at 2578.552 kb on + strand at 2578.552 kb on + strand at 2578.552 kb on + strand at 2578.552 kb on + strand at 2578.552 kb on + strand at 2578.552 kb on + strand at 2578.552 kb on + strand at 2578.553 kb on - strand at 2578.553 kb on - strand at 2578.553 kb on - strand at 2578.553 kb on - strand at 2578.553 kb on - strand at 2578.573 kb on - strand at 2578.583 kb on + strand at 2578.583 kb on + strand at 2578.583 kb on + strand at 2578.583 kb on + strand at 2578.584 kb on - strand at 2578.584 kb on - strand at 2578.584 kb on - strand at 2578.630 kb on + strand at 2578.630 kb on + strand at 2578.630 kb on + strand at 2578.630 kb on + strand at 2578.756 kb on - strand, within Pf1N1B4_2262 at 2578.767 kb on + strand, within Pf1N1B4_2262 at 2578.767 kb on + strand, within Pf1N1B4_2262 at 2578.767 kb on + strand, within Pf1N1B4_2262 at 2578.767 kb on + strand, within Pf1N1B4_2262 at 2578.767 kb on + strand, within Pf1N1B4_2262 at 2578.767 kb on + strand, within Pf1N1B4_2262 at 2578.767 kb on + strand, within Pf1N1B4_2262 at 2578.767 kb on + strand, within Pf1N1B4_2262 at 2578.768 kb on - strand, within Pf1N1B4_2262 at 2578.768 kb on - strand, within Pf1N1B4_2262 at 2578.768 kb on - strand, within Pf1N1B4_2262 at 2578.768 kb on - strand, within Pf1N1B4_2262 at 2578.768 kb on - strand, within Pf1N1B4_2262 at 2578.803 kb on - strand, within Pf1N1B4_2262 at 2578.803 kb on - strand, within Pf1N1B4_2262 at 2578.815 kb on + strand at 2578.815 kb on + strand, within Pf1N1B4_2262 at 2578.815 kb on + strand, within Pf1N1B4_2262 at 2578.815 kb on + strand, within Pf1N1B4_2262 at 2578.815 kb on + strand, within Pf1N1B4_2262 at 2578.815 kb on + strand, within Pf1N1B4_2262 at 2578.815 kb on + strand, within Pf1N1B4_2262 at 2578.816 kb on - strand, within Pf1N1B4_2262 at 2578.842 kb on + strand, within Pf1N1B4_2262 at 2578.842 kb on + strand, within Pf1N1B4_2262 at 2578.842 kb on + strand, within Pf1N1B4_2262 at 2578.842 kb on + strand, within Pf1N1B4_2262 at 2578.842 kb on + strand, within Pf1N1B4_2262 at 2578.842 kb on + strand, within Pf1N1B4_2262 at 2578.842 kb on + strand, within Pf1N1B4_2262 at 2578.842 kb on + strand, within Pf1N1B4_2262 at 2578.843 kb on - strand, within Pf1N1B4_2262 at 2578.843 kb on - strand, within Pf1N1B4_2262 at 2578.843 kb on - strand, within Pf1N1B4_2262 at 2578.843 kb on - strand, within Pf1N1B4_2262 at 2578.843 kb on - strand, within Pf1N1B4_2262 at 2578.843 kb on - strand, within Pf1N1B4_2262 at 2578.854 kb on + strand, within Pf1N1B4_2262 at 2578.855 kb on - strand, within Pf1N1B4_2262 at 2578.924 kb on - strand, within Pf1N1B4_2262 at 2579.013 kb on + strand, within Pf1N1B4_2262 at 2579.014 kb on - strand, within Pf1N1B4_2262 at 2579.014 kb on - strand, within Pf1N1B4_2262 at 2579.014 kb on - strand, within Pf1N1B4_2262 at 2579.121 kb on + strand, within Pf1N1B4_2262 at 2579.122 kb on - strand, within Pf1N1B4_2262 at 2579.122 kb on - strand, within Pf1N1B4_2262 at 2579.122 kb on - strand, within Pf1N1B4_2262 at 2579.136 kb on + strand, within Pf1N1B4_2262 at 2579.136 kb on + strand, within Pf1N1B4_2262 at 2579.137 kb on - strand, within Pf1N1B4_2262 at 2579.137 kb on - strand, within Pf1N1B4_2262 at 2579.137 kb on - strand, within Pf1N1B4_2262 at 2579.142 kb on + strand, within Pf1N1B4_2262 at 2579.142 kb on + strand, within Pf1N1B4_2262 at 2579.142 kb on + strand, within Pf1N1B4_2262 at 2579.143 kb on - strand, within Pf1N1B4_2262 at 2579.143 kb on - strand, within Pf1N1B4_2262 at 2579.480 kb on + strand at 2579.481 kb on - strand at 2579.481 kb on - strand at 2579.564 kb on + strand at 2579.564 kb on + strand at 2579.564 kb on + strand at 2579.564 kb on + strand at 2579.564 kb on + strand at 2579.591 kb on + strand at 2579.592 kb on - strand at 2579.592 kb on - strand at 2579.592 kb on - strand at 2579.648 kb on + strand, within Pf1N1B4_2263 at 2579.648 kb on + strand, within Pf1N1B4_2263 at 2579.648 kb on + strand, within Pf1N1B4_2263 at 2579.649 kb on - strand, within Pf1N1B4_2263 at 2579.714 kb on + strand, within Pf1N1B4_2263 at 2579.770 kb on + strand, within Pf1N1B4_2263 at 2579.954 kb on + strand, within Pf1N1B4_2263 at 2579.955 kb on - strand, within Pf1N1B4_2263 at 2579.955 kb on - strand, within Pf1N1B4_2263 at 2579.955 kb on - strand, within Pf1N1B4_2263 at 2579.955 kb on - strand, within Pf1N1B4_2263 at 2579.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 2,577,691 + Pf1N1B4_2261 0.21 +0.1 2,577,691 + Pf1N1B4_2261 0.21 -0.2 2,577,760 + Pf1N1B4_2261 0.27 +1.4 2,577,887 - Pf1N1B4_2261 0.38 -1.2 2,578,174 + Pf1N1B4_2261 0.63 +0.4 2,578,174 + Pf1N1B4_2261 0.63 +1.0 2,578,174 + Pf1N1B4_2261 0.63 +0.2 2,578,174 + Pf1N1B4_2261 0.63 +1.1 2,578,174 + Pf1N1B4_2261 0.63 -1.9 2,578,300 + Pf1N1B4_2261 0.74 -0.7 2,578,300 + Pf1N1B4_2261 0.74 -0.2 2,578,300 + Pf1N1B4_2261 0.74 -1.6 2,578,300 + Pf1N1B4_2261 0.74 +0.9 2,578,300 + Pf1N1B4_2261 0.74 +1.8 2,578,300 + Pf1N1B4_2261 0.74 +0.1 2,578,300 + Pf1N1B4_2261 0.74 -0.9 2,578,301 - Pf1N1B4_2261 0.74 -0.8 2,578,301 - Pf1N1B4_2261 0.74 +0.7 2,578,301 - Pf1N1B4_2261 0.74 +1.4 2,578,348 + Pf1N1B4_2261 0.78 +0.4 2,578,348 + Pf1N1B4_2261 0.78 +1.0 2,578,349 - Pf1N1B4_2261 0.78 -0.1 2,578,349 - Pf1N1B4_2261 0.78 -0.2 2,578,349 - Pf1N1B4_2261 0.78 +1.2 2,578,438 + Pf1N1B4_2261 0.86 -0.4 2,578,438 + Pf1N1B4_2261 0.86 +0.4 2,578,438 + Pf1N1B4_2261 0.86 -1.2 2,578,462 + Pf1N1B4_2261 0.88 +0.8 2,578,462 + Pf1N1B4_2261 0.88 +0.4 2,578,462 + Pf1N1B4_2261 0.88 -0.2 2,578,462 + Pf1N1B4_2261 0.88 -0.6 2,578,463 - Pf1N1B4_2261 0.88 -0.4 2,578,463 - Pf1N1B4_2261 0.88 +0.4 2,578,463 - Pf1N1B4_2261 0.88 +0.5 2,578,463 - Pf1N1B4_2261 0.88 +1.2 2,578,463 - Pf1N1B4_2261 0.88 +2.0 2,578,552 + +2.4 2,578,552 + -0.2 2,578,552 + -0.5 2,578,552 + +0.4 2,578,552 + +0.7 2,578,552 + -0.8 2,578,552 + +0.9 2,578,552 + -2.0 2,578,552 + -0.6 2,578,553 - -0.5 2,578,553 - -1.8 2,578,553 - +1.1 2,578,553 - +1.4 2,578,553 - +1.5 2,578,573 - -0.5 2,578,583 + -0.0 2,578,583 + +1.4 2,578,583 + +0.4 2,578,583 + -1.6 2,578,584 - -1.2 2,578,584 - -0.2 2,578,584 - +0.8 2,578,630 + -0.3 2,578,630 + -0.8 2,578,630 + +0.8 2,578,630 + +0.9 2,578,756 - Pf1N1B4_2262 0.18 -0.0 2,578,767 + Pf1N1B4_2262 0.19 -0.6 2,578,767 + Pf1N1B4_2262 0.19 +1.6 2,578,767 + Pf1N1B4_2262 0.19 +2.9 2,578,767 + Pf1N1B4_2262 0.19 +0.6 2,578,767 + Pf1N1B4_2262 0.19 -1.0 2,578,767 + Pf1N1B4_2262 0.19 -0.5 2,578,767 + Pf1N1B4_2262 0.19 +1.7 2,578,767 + Pf1N1B4_2262 0.19 +1.8 2,578,768 - Pf1N1B4_2262 0.19 -0.0 2,578,768 - Pf1N1B4_2262 0.19 +0.5 2,578,768 - Pf1N1B4_2262 0.19 +0.2 2,578,768 - Pf1N1B4_2262 0.19 -0.4 2,578,768 - Pf1N1B4_2262 0.19 -1.6 2,578,803 - Pf1N1B4_2262 0.23 -1.9 2,578,803 - Pf1N1B4_2262 0.23 +0.8 2,578,815 + +2.1 2,578,815 + Pf1N1B4_2262 0.24 -2.0 2,578,815 + Pf1N1B4_2262 0.24 -0.8 2,578,815 + Pf1N1B4_2262 0.24 +0.1 2,578,815 + Pf1N1B4_2262 0.24 -0.9 2,578,815 + Pf1N1B4_2262 0.24 -0.8 2,578,815 + Pf1N1B4_2262 0.24 -1.2 2,578,816 - Pf1N1B4_2262 0.24 +0.9 2,578,842 + Pf1N1B4_2262 0.27 -1.7 2,578,842 + Pf1N1B4_2262 0.27 +1.3 2,578,842 + Pf1N1B4_2262 0.27 -1.2 2,578,842 + Pf1N1B4_2262 0.27 +0.1 2,578,842 + Pf1N1B4_2262 0.27 +0.1 2,578,842 + Pf1N1B4_2262 0.27 +0.6 2,578,842 + Pf1N1B4_2262 0.27 -1.6 2,578,842 + Pf1N1B4_2262 0.27 -0.5 2,578,843 - Pf1N1B4_2262 0.27 +0.1 2,578,843 - Pf1N1B4_2262 0.27 +0.3 2,578,843 - Pf1N1B4_2262 0.27 -0.0 2,578,843 - Pf1N1B4_2262 0.27 -1.2 2,578,843 - Pf1N1B4_2262 0.27 +1.3 2,578,843 - Pf1N1B4_2262 0.27 +0.4 2,578,854 + Pf1N1B4_2262 0.28 +0.4 2,578,855 - Pf1N1B4_2262 0.28 -0.1 2,578,924 - Pf1N1B4_2262 0.36 +1.4 2,579,013 + Pf1N1B4_2262 0.46 -0.6 2,579,014 - Pf1N1B4_2262 0.46 +1.4 2,579,014 - Pf1N1B4_2262 0.46 -0.4 2,579,014 - Pf1N1B4_2262 0.46 -2.2 2,579,121 + Pf1N1B4_2262 0.57 +0.3 2,579,122 - Pf1N1B4_2262 0.57 -0.2 2,579,122 - Pf1N1B4_2262 0.57 +1.4 2,579,122 - Pf1N1B4_2262 0.57 +1.1 2,579,136 + Pf1N1B4_2262 0.59 -0.6 2,579,136 + Pf1N1B4_2262 0.59 -0.6 2,579,137 - Pf1N1B4_2262 0.59 -1.0 2,579,137 - Pf1N1B4_2262 0.59 +0.6 2,579,137 - Pf1N1B4_2262 0.59 +1.7 2,579,142 + Pf1N1B4_2262 0.60 +0.5 2,579,142 + Pf1N1B4_2262 0.60 -0.6 2,579,142 + Pf1N1B4_2262 0.60 +0.1 2,579,143 - Pf1N1B4_2262 0.60 +0.7 2,579,143 - Pf1N1B4_2262 0.60 -0.1 2,579,480 + -2.3 2,579,481 - +1.4 2,579,481 - -0.2 2,579,564 + -0.7 2,579,564 + +0.4 2,579,564 + +0.2 2,579,564 + +0.4 2,579,564 + +0.4 2,579,591 + -0.1 2,579,592 - +0.6 2,579,592 - -0.3 2,579,592 - -2.7 2,579,648 + Pf1N1B4_2263 0.15 -1.1 2,579,648 + Pf1N1B4_2263 0.15 +0.6 2,579,648 + Pf1N1B4_2263 0.15 -0.3 2,579,649 - Pf1N1B4_2263 0.16 +0.7 2,579,714 + Pf1N1B4_2263 0.23 +0.1 2,579,770 + Pf1N1B4_2263 0.29 +2.9 2,579,954 + Pf1N1B4_2263 0.50 +0.6 2,579,955 - Pf1N1B4_2263 0.50 +1.9 2,579,955 - Pf1N1B4_2263 0.50 -0.4 2,579,955 - Pf1N1B4_2263 0.50 +0.2 2,579,955 - Pf1N1B4_2263 0.50 +0.3 2,579,955 - +0.4
Or see this region's nucleotide sequence