Experiment: Spectinomycin 0.05 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_2236 and Pf1N1B4_2237 are separated by 163 nucleotides Pf1N1B4_2237 and Pf1N1B4_2238 are separated by 176 nucleotides 
        Pf1N1B4_2236 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 2,548,839 to 2,551,712 
        _2236 
         
        
        Pf1N1B4_2237 - Ribonucleotide reductase, alpha subunit, at 2,551,876 to 2,552,196 
        _2237 
         
        
        Pf1N1B4_2238 - Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB, at 2,552,373 to 2,554,157 
        _2238 
         Position (kb) 2551 
2552 
2553 Strain fitness (log2 ratio) -2 
-1 
0 
1 
2 at 2551.051 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.140 kb on + strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.141 kb on - strand, within Pf1N1B4_2236 at 2551.204 kb on - strand, within Pf1N1B4_2236 at 2551.242 kb on + strand, within Pf1N1B4_2236 at 2551.242 kb on + strand, within Pf1N1B4_2236 at 2551.242 kb on + strand, within Pf1N1B4_2236 at 2551.243 kb on - strand, within Pf1N1B4_2236 at 2551.243 kb on - strand, within Pf1N1B4_2236 at 2551.350 kb on + strand, within Pf1N1B4_2236 at 2551.351 kb on - strand, within Pf1N1B4_2236 at 2551.538 kb on + strand at 2551.538 kb on + strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.539 kb on - strand at 2551.575 kb on + strand at 2551.575 kb on + strand at 2551.576 kb on - strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.622 kb on + strand at 2551.623 kb on - strand at 2551.623 kb on - strand at 2551.623 kb on - strand at 2551.623 kb on - strand at 2551.626 kb on + strand at 2551.626 kb on + strand at 2551.626 kb on + strand at 2551.627 kb on - strand at 2551.659 kb on + strand at 2551.659 kb on + strand at 2551.659 kb on + strand at 2551.820 kb on + strand at 2551.820 kb on + strand at 2551.820 kb on + strand at 2551.820 kb on + strand at 2551.821 kb on - strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.877 kb on + strand at 2551.878 kb on - strand at 2551.970 kb on - strand, within Pf1N1B4_2237 at 2551.986 kb on - strand, within Pf1N1B4_2237 at 2552.075 kb on + strand, within Pf1N1B4_2237 at 2552.075 kb on + strand, within Pf1N1B4_2237 at 2552.075 kb on + strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand, within Pf1N1B4_2237 at 2552.076 kb on - strand at 2552.103 kb on + strand, within Pf1N1B4_2237 at 2552.103 kb on + strand, within Pf1N1B4_2237 at 2552.104 kb on - strand, within Pf1N1B4_2237 at 2552.104 kb on - strand, within Pf1N1B4_2237 at 2552.173 kb on - strand at 2552.196 kb on + strand at 2552.196 kb on + strand at 2552.196 kb on + strand at 2552.196 kb on + strand at 2552.196 kb on + strand at 2552.197 kb on - strand at 2552.197 kb on - strand at 2552.197 kb on - strand at 2552.197 kb on - strand at 2552.219 kb on + strand at 2552.219 kb on + strand at 2552.219 kb on + strand at 2552.219 kb on + strand at 2552.220 kb on - strand at 2552.220 kb on - strand at 2552.220 kb on - strand at 2552.243 kb on + strand at 2552.243 kb on + strand at 2552.374 kb on + strand at 2552.375 kb on - strand at 2552.375 kb on - strand at 2552.375 kb on - strand at 2552.563 kb on + strand, within Pf1N1B4_2238 at 2552.564 kb on - strand, within Pf1N1B4_2238 at 2552.564 kb on - strand, within Pf1N1B4_2238 at 2552.770 kb on + strand, within Pf1N1B4_2238 at 2552.770 kb on + strand, within Pf1N1B4_2238 at 2552.770 kb on + strand, within Pf1N1B4_2238 at 2552.771 kb on - strand, within Pf1N1B4_2238 at 2552.771 kb on - strand, within Pf1N1B4_2238 at 2552.771 kb on - strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.079 kb on + strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238 at 2553.080 kb on - strand, within Pf1N1B4_2238  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Spectinomycin 0.05 mg/ml remove 2,551,051 +  Pf1N1B4_2236 0.77  -0.9 2,551,140 +  Pf1N1B4_2236 0.80  +1.4 2,551,140 +  Pf1N1B4_2236 0.80  -0.9 2,551,140 +  Pf1N1B4_2236 0.80  -0.7 2,551,140 +  Pf1N1B4_2236 0.80  -2.0 2,551,140 +  Pf1N1B4_2236 0.80  +1.6 2,551,140 +  Pf1N1B4_2236 0.80  -1.4 2,551,140 +  Pf1N1B4_2236 0.80  -0.2 2,551,140 +  Pf1N1B4_2236 0.80  -0.2 2,551,140 +  Pf1N1B4_2236 0.80  +1.4 2,551,140 +  Pf1N1B4_2236 0.80  +0.4 2,551,140 +  Pf1N1B4_2236 0.80  +0.1 2,551,140 +  Pf1N1B4_2236 0.80  +1.7 2,551,140 +  Pf1N1B4_2236 0.80  -0.2 2,551,141 -  Pf1N1B4_2236 0.80  -0.4 2,551,141 -  Pf1N1B4_2236 0.80  +1.2 2,551,141 -  Pf1N1B4_2236 0.80  -0.2 2,551,141 -  Pf1N1B4_2236 0.80  +1.6 2,551,141 -  Pf1N1B4_2236 0.80  +1.3 2,551,141 -  Pf1N1B4_2236 0.80  -1.3 2,551,141 -  Pf1N1B4_2236 0.80  -0.4 2,551,141 -  Pf1N1B4_2236 0.80  +0.6 2,551,204 -  Pf1N1B4_2236 0.82  -0.6 2,551,242 +  Pf1N1B4_2236 0.84  -0.6 2,551,242 +  Pf1N1B4_2236 0.84  -1.3 2,551,242 +  Pf1N1B4_2236 0.84  -0.4 2,551,243 -  Pf1N1B4_2236 0.84  +1.4 2,551,243 -  Pf1N1B4_2236 0.84  -0.6 2,551,350 +  Pf1N1B4_2236 0.87  +0.7 2,551,351 -  Pf1N1B4_2236 0.87  -2.2 2,551,538 +  -0.3 2,551,538 +  +0.4 2,551,539 -  -0.6 2,551,539 -  +0.1 2,551,539 -  +0.8 2,551,539 -  +0.6 2,551,539 -  -2.0 2,551,539 -  -2.0 2,551,539 -  +0.9 2,551,539 -  -0.9 2,551,539 -  +0.1 2,551,539 -  -0.1 2,551,539 -  -1.7 2,551,539 -  -0.5 2,551,539 -  +1.4 2,551,575 +  -0.9 2,551,575 +  +0.1 2,551,576 -  +0.8 2,551,622 +  -2.5 2,551,622 +  -1.6 2,551,622 +  +0.0 2,551,622 +  +0.3 2,551,622 +  -0.0 2,551,622 +  +0.9 2,551,623 -  -0.4 2,551,623 -  -1.6 2,551,623 -  +0.7 2,551,623 -  -0.5 2,551,626 +  +0.4 2,551,626 +  -0.6 2,551,626 +  -1.7 2,551,627 -  +0.8 2,551,659 +  -0.3 2,551,659 +  +1.2 2,551,659 +  -1.7 2,551,820 +  +0.4 2,551,820 +  +0.5 2,551,820 +  +0.0 2,551,820 +  -0.4 2,551,821 -  +0.2 2,551,877 +  +1.7 2,551,877 +  +0.3 2,551,877 +  -0.3 2,551,877 +  -0.6 2,551,877 +  +0.3 2,551,877 +  -0.9 2,551,878 -  -0.4 2,551,970 -  Pf1N1B4_2237 0.29  +0.4 2,551,986 -  Pf1N1B4_2237 0.34  -1.8 2,552,075 +  Pf1N1B4_2237 0.62  -0.2 2,552,075 +  Pf1N1B4_2237 0.62  +0.8 2,552,075 +  Pf1N1B4_2237 0.62  -1.6 2,552,076 -  Pf1N1B4_2237 0.62  -0.9 2,552,076 -  Pf1N1B4_2237 0.62  +0.0 2,552,076 -  Pf1N1B4_2237 0.62  -0.6 2,552,076 -  Pf1N1B4_2237 0.62  -0.7 2,552,076 -  Pf1N1B4_2237 0.62  +0.8 2,552,076 -  +0.4 2,552,103 +  Pf1N1B4_2237 0.71  -2.4 2,552,103 +  Pf1N1B4_2237 0.71  -0.2 2,552,104 -  Pf1N1B4_2237 0.71  -0.2 2,552,104 -  Pf1N1B4_2237 0.71  +2.0 2,552,173 -  -0.6 2,552,196 +  +1.2 2,552,196 +  -2.4 2,552,196 +  -2.0 2,552,196 +  +0.0 2,552,196 +  -0.6 2,552,197 -  -0.4 2,552,197 -  +0.4 2,552,197 -  +0.8 2,552,197 -  -1.7 2,552,219 +  -0.2 2,552,219 +  -0.4 2,552,219 +  +0.6 2,552,219 +  -2.2 2,552,220 -  -1.2 2,552,220 -  -2.7 2,552,220 -  +1.0 2,552,243 +  -0.9 2,552,243 +  +1.2 2,552,374 +  -2.4 2,552,375 -  +0.4 2,552,375 -  +1.3 2,552,375 -  +0.1 2,552,563 +  Pf1N1B4_2238 0.11  -0.6 2,552,564 -  Pf1N1B4_2238 0.11  -1.2 2,552,564 -  Pf1N1B4_2238 0.11  +0.7 2,552,770 +  Pf1N1B4_2238 0.22  -0.1 2,552,770 +  Pf1N1B4_2238 0.22  -1.6 2,552,770 +  Pf1N1B4_2238 0.22  +0.1 2,552,771 -  Pf1N1B4_2238 0.22  +0.2 2,552,771 -  Pf1N1B4_2238 0.22  +0.4 2,552,771 -  Pf1N1B4_2238 0.22  +0.3 2,553,079 +  Pf1N1B4_2238 0.40  +0.4 2,553,079 +  Pf1N1B4_2238 0.40  +0.7 2,553,079 +  Pf1N1B4_2238 0.40  -0.2 2,553,079 +  Pf1N1B4_2238 0.40  +0.0 2,553,079 +  Pf1N1B4_2238 0.40  -1.9 2,553,080 -  Pf1N1B4_2238 0.40  -2.7 2,553,080 -  Pf1N1B4_2238 0.40  -0.5 2,553,080 -  Pf1N1B4_2238 0.40  -0.1 2,553,080 -  Pf1N1B4_2238 0.40  -0.2 2,553,080 -  Pf1N1B4_2238 0.40  -0.2 
Or see this region's nucleotide sequence