Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_213 and Pf1N1B4_214 are separated by 202 nucleotides Pf1N1B4_214 and Pf1N1B4_215 overlap by 19 nucleotides
Pf1N1B4_213 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 261,393 to 262,205
_213
Pf1N1B4_214 - Pyoverdine chromophore precursor synthetase PvdL, at 262,408 to 267,885
_214
Pf1N1B4_215 - Pyoverdine chromophore precursor synthetase PvdL, at 267,867 to 275,390
_215
Position (kb) 262
263
264
265
266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 261.462 kb on - strand at 261.462 kb on - strand at 261.487 kb on + strand, within Pf1N1B4_213 at 261.488 kb on - strand, within Pf1N1B4_213 at 261.623 kb on + strand, within Pf1N1B4_213 at 261.749 kb on + strand, within Pf1N1B4_213 at 261.750 kb on - strand, within Pf1N1B4_213 at 261.750 kb on - strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.812 kb on - strand, within Pf1N1B4_213 at 261.812 kb on - strand, within Pf1N1B4_213 at 261.812 kb on - strand at 261.812 kb on - strand, within Pf1N1B4_213 at 261.814 kb on + strand, within Pf1N1B4_213 at 261.847 kb on + strand, within Pf1N1B4_213 at 261.847 kb on + strand, within Pf1N1B4_213 at 261.848 kb on - strand, within Pf1N1B4_213 at 262.106 kb on + strand at 262.106 kb on + strand, within Pf1N1B4_213 at 262.123 kb on + strand, within Pf1N1B4_213 at 262.123 kb on + strand, within Pf1N1B4_213 at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.168 kb on + strand at 262.168 kb on + strand at 262.168 kb on + strand at 262.169 kb on - strand at 262.169 kb on - strand at 262.226 kb on + strand at 262.227 kb on - strand at 262.227 kb on - strand at 262.227 kb on - strand at 262.230 kb on - strand at 262.289 kb on + strand at 262.289 kb on + strand at 262.289 kb on + strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.409 kb on + strand at 262.410 kb on - strand at 262.455 kb on + strand at 262.494 kb on - strand at 262.668 kb on + strand at 262.823 kb on + strand at 262.823 kb on + strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.908 kb on - strand at 262.908 kb on - strand at 262.908 kb on - strand at 263.102 kb on + strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214 at 263.703 kb on - strand, within Pf1N1B4_214 at 263.787 kb on - strand, within Pf1N1B4_214 at 263.787 kb on - strand, within Pf1N1B4_214 at 263.790 kb on + strand, within Pf1N1B4_214 at 263.790 kb on + strand, within Pf1N1B4_214 at 263.819 kb on + strand, within Pf1N1B4_214 at 263.820 kb on - strand, within Pf1N1B4_214 at 263.820 kb on - strand, within Pf1N1B4_214 at 264.019 kb on + strand, within Pf1N1B4_214 at 264.020 kb on - strand, within Pf1N1B4_214 at 264.023 kb on + strand, within Pf1N1B4_214 at 264.171 kb on - strand, within Pf1N1B4_214 at 264.171 kb on - strand at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand at 264.248 kb on + strand at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.425 kb on + strand, within Pf1N1B4_214 at 264.426 kb on - strand, within Pf1N1B4_214 at 264.438 kb on - strand at 264.438 kb on - strand, within Pf1N1B4_214 at 264.612 kb on + strand, within Pf1N1B4_214 at 264.653 kb on + strand, within Pf1N1B4_214 at 264.653 kb on + strand at 264.654 kb on - strand, within Pf1N1B4_214 at 264.654 kb on - strand, within Pf1N1B4_214 at 264.660 kb on - strand, within Pf1N1B4_214 at 264.963 kb on + strand, within Pf1N1B4_214 at 265.121 kb on + strand, within Pf1N1B4_214 at 265.122 kb on - strand, within Pf1N1B4_214 at 265.181 kb on + strand, within Pf1N1B4_214 at 265.181 kb on + strand at 265.182 kb on - strand, within Pf1N1B4_214 at 265.182 kb on - strand, within Pf1N1B4_214 at 265.403 kb on + strand, within Pf1N1B4_214 at 265.404 kb on - strand, within Pf1N1B4_214 at 265.571 kb on + strand, within Pf1N1B4_214 at 265.571 kb on + strand, within Pf1N1B4_214 at 265.571 kb on + strand, within Pf1N1B4_214 at 265.572 kb on - strand at 265.572 kb on - strand, within Pf1N1B4_214 at 265.572 kb on - strand at 265.572 kb on - strand, within Pf1N1B4_214 at 265.574 kb on + strand, within Pf1N1B4_214 at 265.784 kb on + strand, within Pf1N1B4_214 at 265.865 kb on + strand, within Pf1N1B4_214 at 265.992 kb on - strand, within Pf1N1B4_214 at 265.992 kb on - strand at 266.278 kb on + strand, within Pf1N1B4_214 at 266.279 kb on - strand, within Pf1N1B4_214 at 266.279 kb on - strand, within Pf1N1B4_214 at 266.279 kb on - strand, within Pf1N1B4_214 at 266.280 kb on - strand, within Pf1N1B4_214 at 266.566 kb on + strand, within Pf1N1B4_214 at 267.293 kb on + strand, within Pf1N1B4_214 at 267.293 kb on + strand, within Pf1N1B4_214 at 267.293 kb on + strand, within Pf1N1B4_214 at 267.294 kb on - strand, within Pf1N1B4_214 at 267.404 kb on + strand at 267.404 kb on + strand at 267.405 kb on - strand at 267.644 kb on + strand at 267.795 kb on - strand at 267.795 kb on - strand at 267.848 kb on + strand at 267.848 kb on + strand at 267.848 kb on + strand at 267.849 kb on - strand at 267.849 kb on - strand at 267.959 kb on - strand at 268.102 kb on + strand at 268.102 kb on + strand at 268.102 kb on + strand at 268.134 kb on - strand at 268.258 kb on + strand at 268.259 kb on - strand at 268.259 kb on - strand at 268.381 kb on + strand at 268.390 kb on + strand at 268.416 kb on - strand at 268.564 kb on + strand at 268.564 kb on + strand at 268.564 kb on + strand at 268.565 kb on - strand at 268.565 kb on - strand at 268.606 kb on + strand at 268.607 kb on - strand at 268.607 kb on - strand at 268.607 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 261,462 - -1.2 261,462 - +0.5 261,487 + Pf1N1B4_213 0.12 -0.0 261,488 - Pf1N1B4_213 0.12 -1.5 261,623 + Pf1N1B4_213 0.28 +0.5 261,749 + Pf1N1B4_213 0.44 +0.7 261,750 - Pf1N1B4_213 0.44 +0.4 261,750 - Pf1N1B4_213 0.44 -0.7 261,811 + Pf1N1B4_213 0.51 +0.8 261,811 + Pf1N1B4_213 0.51 -2.0 261,811 + Pf1N1B4_213 0.51 -0.4 261,812 - Pf1N1B4_213 0.52 +0.8 261,812 - Pf1N1B4_213 0.52 -0.2 261,812 - -1.8 261,812 - Pf1N1B4_213 0.52 -1.5 261,814 + Pf1N1B4_213 0.52 +0.2 261,847 + Pf1N1B4_213 0.56 +1.7 261,847 + Pf1N1B4_213 0.56 -0.5 261,848 - Pf1N1B4_213 0.56 +1.4 262,106 + +0.8 262,106 + Pf1N1B4_213 0.88 -0.2 262,123 + Pf1N1B4_213 0.90 +0.0 262,123 + Pf1N1B4_213 0.90 +1.8 262,164 + +1.0 262,164 + +0.4 262,164 + +1.8 262,164 + +0.4 262,164 + -0.8 262,164 + -0.4 262,165 - +1.4 262,165 - +0.2 262,165 - +0.3 262,165 - +0.8 262,165 - +0.4 262,165 - +0.5 262,168 + -0.8 262,168 + -0.8 262,168 + +2.2 262,169 - -1.2 262,169 - -0.2 262,226 + -0.1 262,227 - -1.9 262,227 - +0.8 262,227 - -1.5 262,230 - -1.5 262,289 + -0.2 262,289 + +0.0 262,289 + +0.6 262,290 - -0.2 262,290 - +0.4 262,290 - -2.8 262,290 - -0.1 262,290 - -1.6 262,290 - -0.2 262,409 + +0.5 262,410 - +0.4 262,455 + -1.2 262,494 - +1.3 262,668 + -0.0 262,823 + -0.2 262,823 + -0.2 262,824 - +0.7 262,824 - +0.4 262,824 - -0.2 262,824 - +0.1 262,824 - -0.5 262,824 - -0.4 262,908 - -1.2 262,908 - -2.0 262,908 - -1.5 263,102 + Pf1N1B4_214 0.13 -0.4 263,175 - Pf1N1B4_214 0.14 -1.2 263,175 - Pf1N1B4_214 0.14 +0.6 263,175 - Pf1N1B4_214 0.14 -0.5 263,703 - Pf1N1B4_214 0.24 +1.0 263,787 - Pf1N1B4_214 0.25 +0.4 263,787 - Pf1N1B4_214 0.25 -2.0 263,790 + Pf1N1B4_214 0.25 +1.2 263,790 + Pf1N1B4_214 0.25 -0.2 263,819 + Pf1N1B4_214 0.26 -0.3 263,820 - Pf1N1B4_214 0.26 -1.5 263,820 - Pf1N1B4_214 0.26 +1.7 264,019 + Pf1N1B4_214 0.29 +1.3 264,020 - Pf1N1B4_214 0.29 +1.3 264,023 + Pf1N1B4_214 0.29 +1.8 264,171 - Pf1N1B4_214 0.32 -0.7 264,171 - +0.2 264,248 + Pf1N1B4_214 0.34 -2.0 264,248 + Pf1N1B4_214 0.34 -0.2 264,248 + Pf1N1B4_214 0.34 +0.4 264,248 + Pf1N1B4_214 0.34 +0.8 264,248 + Pf1N1B4_214 0.34 -0.4 264,248 + +0.8 264,248 + +0.4 264,248 + Pf1N1B4_214 0.34 +1.0 264,248 + Pf1N1B4_214 0.34 +0.8 264,249 - Pf1N1B4_214 0.34 +0.5 264,249 - Pf1N1B4_214 0.34 +0.5 264,249 - Pf1N1B4_214 0.34 -0.2 264,249 - Pf1N1B4_214 0.34 +0.4 264,249 - Pf1N1B4_214 0.34 -2.2 264,249 - Pf1N1B4_214 0.34 +1.1 264,249 - Pf1N1B4_214 0.34 +0.8 264,249 - -0.2 264,249 - Pf1N1B4_214 0.34 -2.2 264,249 - Pf1N1B4_214 0.34 -2.0 264,249 - Pf1N1B4_214 0.34 -1.5 264,425 + Pf1N1B4_214 0.37 +0.8 264,426 - Pf1N1B4_214 0.37 +0.8 264,438 - -0.8 264,438 - Pf1N1B4_214 0.37 +2.3 264,612 + Pf1N1B4_214 0.40 -2.7 264,653 + Pf1N1B4_214 0.41 -0.2 264,653 + -0.2 264,654 - Pf1N1B4_214 0.41 +0.2 264,654 - Pf1N1B4_214 0.41 +0.3 264,660 - Pf1N1B4_214 0.41 +1.2 264,963 + Pf1N1B4_214 0.47 +0.8 265,121 + Pf1N1B4_214 0.50 -0.2 265,122 - Pf1N1B4_214 0.50 +1.8 265,181 + Pf1N1B4_214 0.51 -1.2 265,181 + -0.8 265,182 - Pf1N1B4_214 0.51 -1.2 265,182 - Pf1N1B4_214 0.51 +0.7 265,403 + Pf1N1B4_214 0.55 -0.1 265,404 - Pf1N1B4_214 0.55 -0.2 265,571 + Pf1N1B4_214 0.58 +0.8 265,571 + Pf1N1B4_214 0.58 +0.8 265,571 + Pf1N1B4_214 0.58 -2.0 265,572 - -0.2 265,572 - Pf1N1B4_214 0.58 +0.5 265,572 - +1.8 265,572 - Pf1N1B4_214 0.58 -1.8 265,574 + Pf1N1B4_214 0.58 -1.2 265,784 + Pf1N1B4_214 0.62 +1.1 265,865 + Pf1N1B4_214 0.63 -0.4 265,992 - Pf1N1B4_214 0.65 -1.8 265,992 - +1.2 266,278 + Pf1N1B4_214 0.71 +0.5 266,279 - Pf1N1B4_214 0.71 +0.8 266,279 - Pf1N1B4_214 0.71 +0.2 266,279 - Pf1N1B4_214 0.71 +2.9 266,280 - Pf1N1B4_214 0.71 -0.2 266,566 + Pf1N1B4_214 0.76 -0.5 267,293 + Pf1N1B4_214 0.89 +0.6 267,293 + Pf1N1B4_214 0.89 +0.5 267,293 + Pf1N1B4_214 0.89 +0.2 267,294 - Pf1N1B4_214 0.89 +1.4 267,404 + -1.7 267,404 + -0.2 267,405 - +0.5 267,644 + -0.8 267,795 - +0.2 267,795 - +0.3 267,848 + +1.1 267,848 + -0.6 267,848 + -0.2 267,849 - -1.8 267,849 - +0.1 267,959 - +1.0 268,102 + +0.4 268,102 + +1.3 268,102 + +1.8 268,134 - -1.5 268,258 + -1.2 268,259 - +0.1 268,259 - -0.1 268,381 + +0.2 268,390 + -0.2 268,416 - +1.4 268,564 + +0.4 268,564 + +0.3 268,564 + +0.1 268,565 - +0.1 268,565 - +1.4 268,606 + +1.5 268,607 - -1.0 268,607 - +0.4 268,607 - -0.9
Or see this region's nucleotide sequence