Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2033 and Pf1N1B4_2034 are separated by 239 nucleotides Pf1N1B4_2034 and Pf1N1B4_2035 are separated by 234 nucleotides
Pf1N1B4_2033 - L-Proline/Glycine betaine transporter ProP, at 2,319,273 to 2,320,571
_2033
Pf1N1B4_2034 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 2,320,811 to 2,321,809
_2034
Pf1N1B4_2035 - Phosphate transport system permease protein PstC (TC 3.A.1.7.1), at 2,322,044 to 2,324,332
_2035
Position (kb)
2320
2321
2322 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2319.840 kb on + strand, within Pf1N1B4_2033 at 2319.840 kb on + strand, within Pf1N1B4_2033 at 2319.841 kb on - strand, within Pf1N1B4_2033 at 2319.841 kb on - strand, within Pf1N1B4_2033 at 2319.841 kb on - strand, within Pf1N1B4_2033 at 2319.841 kb on - strand, within Pf1N1B4_2033 at 2319.919 kb on - strand, within Pf1N1B4_2033 at 2319.919 kb on - strand, within Pf1N1B4_2033 at 2320.009 kb on + strand, within Pf1N1B4_2033 at 2320.009 kb on + strand, within Pf1N1B4_2033 at 2320.009 kb on + strand, within Pf1N1B4_2033 at 2320.009 kb on + strand, within Pf1N1B4_2033 at 2320.010 kb on - strand, within Pf1N1B4_2033 at 2320.010 kb on - strand, within Pf1N1B4_2033 at 2320.010 kb on - strand, within Pf1N1B4_2033 at 2320.010 kb on - strand, within Pf1N1B4_2033 at 2320.010 kb on - strand, within Pf1N1B4_2033 at 2320.103 kb on - strand, within Pf1N1B4_2033 at 2320.103 kb on - strand at 2320.116 kb on + strand, within Pf1N1B4_2033 at 2320.228 kb on + strand, within Pf1N1B4_2033 at 2320.228 kb on + strand, within Pf1N1B4_2033 at 2320.234 kb on + strand, within Pf1N1B4_2033 at 2320.275 kb on + strand, within Pf1N1B4_2033 at 2320.275 kb on + strand, within Pf1N1B4_2033 at 2320.276 kb on - strand, within Pf1N1B4_2033 at 2320.276 kb on - strand, within Pf1N1B4_2033 at 2320.317 kb on + strand, within Pf1N1B4_2033 at 2320.317 kb on + strand, within Pf1N1B4_2033 at 2320.317 kb on + strand, within Pf1N1B4_2033 at 2320.317 kb on + strand, within Pf1N1B4_2033 at 2320.318 kb on - strand, within Pf1N1B4_2033 at 2320.318 kb on - strand, within Pf1N1B4_2033 at 2320.318 kb on - strand, within Pf1N1B4_2033 at 2320.318 kb on - strand, within Pf1N1B4_2033 at 2320.318 kb on - strand, within Pf1N1B4_2033 at 2320.324 kb on + strand, within Pf1N1B4_2033 at 2320.325 kb on - strand, within Pf1N1B4_2033 at 2320.339 kb on + strand, within Pf1N1B4_2033 at 2320.362 kb on + strand, within Pf1N1B4_2033 at 2320.362 kb on + strand, within Pf1N1B4_2033 at 2320.362 kb on + strand, within Pf1N1B4_2033 at 2320.362 kb on + strand, within Pf1N1B4_2033 at 2320.362 kb on + strand, within Pf1N1B4_2033 at 2320.362 kb on + strand, within Pf1N1B4_2033 at 2320.363 kb on - strand, within Pf1N1B4_2033 at 2320.420 kb on - strand, within Pf1N1B4_2033 at 2320.420 kb on - strand, within Pf1N1B4_2033 at 2320.503 kb on + strand at 2320.503 kb on + strand at 2320.503 kb on + strand at 2320.503 kb on + strand at 2320.504 kb on - strand at 2320.504 kb on - strand at 2320.504 kb on - strand at 2320.504 kb on - strand at 2320.504 kb on - strand at 2320.506 kb on + strand at 2320.506 kb on + strand at 2320.506 kb on + strand at 2320.506 kb on + strand at 2320.506 kb on + strand at 2320.506 kb on + strand at 2320.506 kb on + strand at 2320.507 kb on - strand at 2320.507 kb on - strand at 2320.507 kb on - strand at 2320.507 kb on - strand at 2320.507 kb on - strand at 2320.548 kb on + strand at 2320.548 kb on + strand at 2320.549 kb on - strand at 2320.549 kb on - strand at 2320.549 kb on - strand at 2320.699 kb on + strand at 2320.699 kb on + strand at 2320.705 kb on + strand at 2320.705 kb on + strand at 2320.706 kb on - strand at 2320.706 kb on - strand at 2320.706 kb on - strand at 2320.706 kb on - strand at 2320.733 kb on + strand at 2320.744 kb on + strand at 2320.744 kb on + strand at 2320.744 kb on + strand at 2320.744 kb on + strand at 2320.744 kb on + strand at 2320.744 kb on + strand at 2320.745 kb on - strand at 2320.745 kb on - strand at 2320.745 kb on - strand at 2320.745 kb on - strand at 2320.783 kb on + strand at 2320.783 kb on + strand at 2320.792 kb on + strand at 2320.792 kb on + strand at 2320.792 kb on + strand at 2320.792 kb on + strand at 2320.792 kb on + strand at 2320.793 kb on - strand at 2320.793 kb on - strand at 2320.799 kb on + strand at 2320.799 kb on + strand at 2320.799 kb on + strand at 2320.805 kb on + strand at 2321.016 kb on + strand at 2321.726 kb on + strand at 2321.726 kb on + strand at 2321.727 kb on - strand at 2321.796 kb on + strand at 2321.796 kb on + strand at 2321.796 kb on + strand at 2321.796 kb on + strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.797 kb on - strand at 2321.799 kb on + strand at 2321.799 kb on + strand at 2321.799 kb on + strand at 2321.799 kb on + strand at 2321.815 kb on + strand at 2321.897 kb on + strand at 2321.897 kb on + strand at 2321.897 kb on + strand at 2321.897 kb on + strand at 2321.897 kb on + strand at 2321.898 kb on - strand at 2321.898 kb on - strand at 2321.898 kb on - strand at 2321.898 kb on - strand at 2321.929 kb on - strand at 2322.027 kb on + strand at 2322.027 kb on + strand at 2322.110 kb on - strand at 2322.590 kb on + strand, within Pf1N1B4_2035
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 2,319,840 + Pf1N1B4_2033 0.44 -1.1 2,319,840 + Pf1N1B4_2033 0.44 -1.0 2,319,841 - Pf1N1B4_2033 0.44 -0.3 2,319,841 - Pf1N1B4_2033 0.44 +1.3 2,319,841 - Pf1N1B4_2033 0.44 +1.2 2,319,841 - Pf1N1B4_2033 0.44 +1.0 2,319,919 - Pf1N1B4_2033 0.50 -1.5 2,319,919 - Pf1N1B4_2033 0.50 +1.2 2,320,009 + Pf1N1B4_2033 0.57 +0.8 2,320,009 + Pf1N1B4_2033 0.57 -0.8 2,320,009 + Pf1N1B4_2033 0.57 +2.5 2,320,009 + Pf1N1B4_2033 0.57 +0.6 2,320,010 - Pf1N1B4_2033 0.57 +3.2 2,320,010 - Pf1N1B4_2033 0.57 -0.8 2,320,010 - Pf1N1B4_2033 0.57 +0.2 2,320,010 - Pf1N1B4_2033 0.57 -0.8 2,320,010 - Pf1N1B4_2033 0.57 -0.3 2,320,103 - Pf1N1B4_2033 0.64 +0.4 2,320,103 - +1.5 2,320,116 + Pf1N1B4_2033 0.65 +0.6 2,320,228 + Pf1N1B4_2033 0.74 -0.9 2,320,228 + Pf1N1B4_2033 0.74 +1.7 2,320,234 + Pf1N1B4_2033 0.74 +0.5 2,320,275 + Pf1N1B4_2033 0.77 +0.6 2,320,275 + Pf1N1B4_2033 0.77 +0.3 2,320,276 - Pf1N1B4_2033 0.77 +1.2 2,320,276 - Pf1N1B4_2033 0.77 +0.6 2,320,317 + Pf1N1B4_2033 0.80 +0.2 2,320,317 + Pf1N1B4_2033 0.80 -1.3 2,320,317 + Pf1N1B4_2033 0.80 -2.2 2,320,317 + Pf1N1B4_2033 0.80 -0.3 2,320,318 - Pf1N1B4_2033 0.80 +1.6 2,320,318 - Pf1N1B4_2033 0.80 -0.1 2,320,318 - Pf1N1B4_2033 0.80 -0.0 2,320,318 - Pf1N1B4_2033 0.80 +0.5 2,320,318 - Pf1N1B4_2033 0.80 -1.3 2,320,324 + Pf1N1B4_2033 0.81 +0.5 2,320,325 - Pf1N1B4_2033 0.81 +0.8 2,320,339 + Pf1N1B4_2033 0.82 +1.2 2,320,362 + Pf1N1B4_2033 0.84 +1.9 2,320,362 + Pf1N1B4_2033 0.84 -0.6 2,320,362 + Pf1N1B4_2033 0.84 -2.0 2,320,362 + Pf1N1B4_2033 0.84 +0.6 2,320,362 + Pf1N1B4_2033 0.84 -0.5 2,320,362 + Pf1N1B4_2033 0.84 -0.5 2,320,363 - Pf1N1B4_2033 0.84 +0.4 2,320,420 - Pf1N1B4_2033 0.88 +0.0 2,320,420 - Pf1N1B4_2033 0.88 -1.0 2,320,503 + -1.3 2,320,503 + -0.1 2,320,503 + -1.1 2,320,503 + +0.8 2,320,504 - -0.8 2,320,504 - -0.4 2,320,504 - -0.3 2,320,504 - +0.8 2,320,504 - +1.5 2,320,506 + +1.3 2,320,506 + +0.5 2,320,506 + -1.7 2,320,506 + -0.9 2,320,506 + -0.0 2,320,506 + -0.3 2,320,506 + -1.4 2,320,507 - -1.7 2,320,507 - +1.0 2,320,507 - +1.0 2,320,507 - +0.5 2,320,507 - -0.5 2,320,548 + -0.1 2,320,548 + +0.9 2,320,549 - -0.1 2,320,549 - -0.3 2,320,549 - +0.1 2,320,699 + -0.8 2,320,699 + +1.1 2,320,705 + -0.5 2,320,705 + +0.5 2,320,706 - +1.1 2,320,706 - -0.1 2,320,706 - -0.4 2,320,706 - -0.6 2,320,733 + +0.5 2,320,744 + -0.7 2,320,744 + -1.8 2,320,744 + +1.0 2,320,744 + -0.2 2,320,744 + +0.2 2,320,744 + +0.1 2,320,745 - -0.8 2,320,745 - +0.1 2,320,745 - +1.2 2,320,745 - +2.2 2,320,783 + -1.5 2,320,783 + -0.1 2,320,792 + +0.5 2,320,792 + +1.9 2,320,792 + +0.2 2,320,792 + +2.5 2,320,792 + -0.1 2,320,793 - -1.5 2,320,793 - +1.2 2,320,799 + -0.8 2,320,799 + +0.9 2,320,799 + +1.9 2,320,805 + +0.0 2,321,016 + -0.1 2,321,726 + +0.5 2,321,726 + -1.8 2,321,727 - -1.8 2,321,796 + +0.9 2,321,796 + -0.8 2,321,796 + +0.6 2,321,796 + +1.3 2,321,797 - +1.1 2,321,797 - +1.5 2,321,797 - -0.8 2,321,797 - +0.5 2,321,797 - +0.7 2,321,797 - +2.1 2,321,797 - -0.3 2,321,797 - -0.4 2,321,799 + -0.0 2,321,799 + -0.5 2,321,799 + -0.1 2,321,799 + +0.2 2,321,815 + +1.7 2,321,897 + -1.3 2,321,897 + -1.5 2,321,897 + -0.4 2,321,897 + +0.5 2,321,897 + +0.2 2,321,898 - +0.3 2,321,898 - +0.5 2,321,898 - -1.2 2,321,898 - +0.1 2,321,929 - +0.2 2,322,027 + +1.0 2,322,027 + -0.8 2,322,110 - -2.7 2,322,590 + Pf1N1B4_2035 0.24 +0.1
Or see this region's nucleotide sequence