Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2029 and Pf1N1B4_2030 are separated by 42 nucleotides Pf1N1B4_2030 and Pf1N1B4_2031 are separated by 121 nucleotides Pf1N1B4_2031 and Pf1N1B4_2032 are separated by 85 nucleotides Pf1N1B4_2032 and Pf1N1B4_2033 are separated by 367 nucleotides
Pf1N1B4_2029 - FIG00953722: hypothetical protein, at 2,316,522 to 2,316,773
_2029
Pf1N1B4_2030 - lipoprotein, putative, at 2,316,816 to 2,317,394
_2030
Pf1N1B4_2031 - Aldose 1-epimerase, at 2,317,516 to 2,318,415
_2031
Pf1N1B4_2032 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 2,318,501 to 2,318,905
_2032
Pf1N1B4_2033 - L-Proline/Glycine betaine transporter ProP, at 2,319,273 to 2,320,571
_2033
Position (kb)
2317
2318
2319 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2316.679 kb on - strand, within Pf1N1B4_2029 at 2316.679 kb on - strand, within Pf1N1B4_2029 at 2316.730 kb on + strand, within Pf1N1B4_2029 at 2316.744 kb on + strand, within Pf1N1B4_2029 at 2316.744 kb on + strand, within Pf1N1B4_2029 at 2316.744 kb on + strand, within Pf1N1B4_2029 at 2316.745 kb on - strand, within Pf1N1B4_2029 at 2316.813 kb on - strand at 2316.813 kb on - strand at 2316.813 kb on - strand at 2316.813 kb on - strand at 2316.813 kb on - strand at 2317.071 kb on + strand, within Pf1N1B4_2030 at 2317.071 kb on + strand, within Pf1N1B4_2030 at 2317.071 kb on + strand, within Pf1N1B4_2030 at 2317.071 kb on + strand, within Pf1N1B4_2030 at 2317.071 kb on + strand, within Pf1N1B4_2030 at 2317.071 kb on + strand, within Pf1N1B4_2030 at 2317.072 kb on - strand, within Pf1N1B4_2030 at 2317.072 kb on - strand, within Pf1N1B4_2030 at 2317.072 kb on - strand, within Pf1N1B4_2030 at 2317.072 kb on - strand, within Pf1N1B4_2030 at 2317.072 kb on - strand, within Pf1N1B4_2030 at 2317.072 kb on - strand, within Pf1N1B4_2030 at 2317.072 kb on - strand, within Pf1N1B4_2030 at 2317.264 kb on + strand, within Pf1N1B4_2030 at 2317.264 kb on + strand, within Pf1N1B4_2030 at 2317.264 kb on + strand, within Pf1N1B4_2030 at 2317.264 kb on + strand, within Pf1N1B4_2030 at 2317.264 kb on + strand, within Pf1N1B4_2030 at 2317.264 kb on + strand, within Pf1N1B4_2030 at 2317.265 kb on - strand, within Pf1N1B4_2030 at 2317.265 kb on - strand, within Pf1N1B4_2030 at 2317.265 kb on - strand, within Pf1N1B4_2030 at 2317.275 kb on + strand, within Pf1N1B4_2030 at 2317.275 kb on + strand, within Pf1N1B4_2030 at 2317.275 kb on + strand, within Pf1N1B4_2030 at 2317.275 kb on + strand, within Pf1N1B4_2030 at 2317.275 kb on + strand, within Pf1N1B4_2030 at 2317.275 kb on + strand, within Pf1N1B4_2030 at 2317.276 kb on - strand, within Pf1N1B4_2030 at 2317.276 kb on - strand, within Pf1N1B4_2030 at 2317.276 kb on - strand, within Pf1N1B4_2030 at 2317.276 kb on - strand, within Pf1N1B4_2030 at 2317.276 kb on - strand, within Pf1N1B4_2030 at 2317.393 kb on - strand at 2317.406 kb on + strand at 2317.407 kb on - strand at 2317.448 kb on + strand at 2317.448 kb on + strand at 2317.449 kb on - strand at 2317.449 kb on - strand at 2317.518 kb on - strand at 2317.518 kb on - strand at 2317.579 kb on + strand at 2317.579 kb on + strand at 2317.579 kb on + strand at 2317.579 kb on + strand at 2317.579 kb on + strand at 2317.580 kb on - strand at 2317.580 kb on - strand at 2317.580 kb on - strand at 2317.580 kb on - strand at 2317.580 kb on - strand at 2317.580 kb on - strand at 2317.580 kb on - strand at 2317.742 kb on - strand at 2317.772 kb on + strand, within Pf1N1B4_2031 at 2317.772 kb on + strand, within Pf1N1B4_2031 at 2317.772 kb on + strand, within Pf1N1B4_2031 at 2317.772 kb on + strand, within Pf1N1B4_2031 at 2317.772 kb on + strand, within Pf1N1B4_2031 at 2317.772 kb on + strand, within Pf1N1B4_2031 at 2317.773 kb on - strand, within Pf1N1B4_2031 at 2317.773 kb on - strand, within Pf1N1B4_2031 at 2317.773 kb on - strand, within Pf1N1B4_2031 at 2317.773 kb on - strand, within Pf1N1B4_2031 at 2317.850 kb on + strand, within Pf1N1B4_2031 at 2317.850 kb on + strand, within Pf1N1B4_2031 at 2317.850 kb on + strand, within Pf1N1B4_2031 at 2317.850 kb on + strand, within Pf1N1B4_2031 at 2317.850 kb on + strand, within Pf1N1B4_2031 at 2317.850 kb on + strand, within Pf1N1B4_2031 at 2317.850 kb on + strand, within Pf1N1B4_2031 at 2317.851 kb on - strand, within Pf1N1B4_2031 at 2317.851 kb on - strand, within Pf1N1B4_2031 at 2317.851 kb on - strand, within Pf1N1B4_2031 at 2317.851 kb on - strand, within Pf1N1B4_2031 at 2317.879 kb on + strand, within Pf1N1B4_2031 at 2317.879 kb on + strand, within Pf1N1B4_2031 at 2317.879 kb on + strand, within Pf1N1B4_2031 at 2317.880 kb on - strand, within Pf1N1B4_2031 at 2317.880 kb on - strand, within Pf1N1B4_2031 at 2317.880 kb on - strand, within Pf1N1B4_2031 at 2317.880 kb on - strand, within Pf1N1B4_2031 at 2317.880 kb on - strand, within Pf1N1B4_2031 at 2317.880 kb on - strand, within Pf1N1B4_2031 at 2317.880 kb on - strand, within Pf1N1B4_2031 at 2317.907 kb on + strand, within Pf1N1B4_2031 at 2317.907 kb on + strand, within Pf1N1B4_2031 at 2317.907 kb on + strand, within Pf1N1B4_2031 at 2317.907 kb on + strand, within Pf1N1B4_2031 at 2317.907 kb on + strand, within Pf1N1B4_2031 at 2317.907 kb on + strand, within Pf1N1B4_2031 at 2317.908 kb on - strand, within Pf1N1B4_2031 at 2317.908 kb on - strand, within Pf1N1B4_2031 at 2317.908 kb on - strand, within Pf1N1B4_2031 at 2317.908 kb on - strand, within Pf1N1B4_2031 at 2317.971 kb on + strand, within Pf1N1B4_2031 at 2317.972 kb on - strand, within Pf1N1B4_2031 at 2318.304 kb on - strand, within Pf1N1B4_2031 at 2318.489 kb on - strand at 2318.900 kb on + strand at 2318.942 kb on - strand at 2318.942 kb on - strand at 2318.942 kb on - strand at 2318.942 kb on - strand at 2318.942 kb on - strand at 2318.956 kb on - strand at 2318.966 kb on - strand at 2318.966 kb on - strand at 2318.966 kb on - strand at 2318.966 kb on - strand at 2318.966 kb on - strand at 2318.966 kb on - strand at 2318.966 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.992 kb on - strand at 2318.994 kb on + strand at 2318.994 kb on + strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2318.995 kb on - strand at 2319.086 kb on - strand at 2319.101 kb on - strand at 2319.109 kb on + strand at 2319.110 kb on - strand at 2319.110 kb on - strand at 2319.110 kb on - strand at 2319.110 kb on - strand at 2319.110 kb on - strand at 2319.110 kb on - strand at 2319.110 kb on - strand at 2319.110 kb on - strand at 2319.110 kb on - strand at 2319.112 kb on - strand at 2319.165 kb on - strand at 2319.165 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.175 kb on - strand at 2319.254 kb on + strand at 2319.255 kb on - strand at 2319.255 kb on - strand at 2319.255 kb on - strand at 2319.255 kb on - strand at 2319.256 kb on + strand at 2319.256 kb on + strand at 2319.256 kb on + strand at 2319.256 kb on + strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.257 kb on - strand at 2319.339 kb on + strand at 2319.339 kb on + strand at 2319.339 kb on + strand at 2319.340 kb on - strand at 2319.340 kb on - strand at 2319.340 kb on - strand at 2319.340 kb on - strand at 2319.340 kb on - strand at 2319.340 kb on - strand at 2319.340 kb on - strand at 2319.340 kb on - strand at 2319.341 kb on + strand at 2319.341 kb on + strand at 2319.341 kb on + strand at 2319.341 kb on + strand at 2319.341 kb on + strand at 2319.341 kb on + strand at 2319.341 kb on + strand at 2319.341 kb on + strand at 2319.341 kb on + strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.342 kb on - strand at 2319.385 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 2,316,679 - Pf1N1B4_2029 0.62 -1.3 2,316,679 - Pf1N1B4_2029 0.62 +1.1 2,316,730 + Pf1N1B4_2029 0.83 +0.2 2,316,744 + Pf1N1B4_2029 0.88 +1.1 2,316,744 + Pf1N1B4_2029 0.88 -0.3 2,316,744 + Pf1N1B4_2029 0.88 -1.7 2,316,745 - Pf1N1B4_2029 0.88 +1.2 2,316,813 - -0.5 2,316,813 - -1.1 2,316,813 - +1.7 2,316,813 - +0.5 2,316,813 - -0.8 2,317,071 + Pf1N1B4_2030 0.44 +0.3 2,317,071 + Pf1N1B4_2030 0.44 +0.5 2,317,071 + Pf1N1B4_2030 0.44 -1.3 2,317,071 + Pf1N1B4_2030 0.44 -0.1 2,317,071 + Pf1N1B4_2030 0.44 -1.8 2,317,071 + Pf1N1B4_2030 0.44 +0.3 2,317,072 - Pf1N1B4_2030 0.44 +1.9 2,317,072 - Pf1N1B4_2030 0.44 +0.7 2,317,072 - Pf1N1B4_2030 0.44 -0.2 2,317,072 - Pf1N1B4_2030 0.44 -0.8 2,317,072 - Pf1N1B4_2030 0.44 -1.7 2,317,072 - Pf1N1B4_2030 0.44 -0.5 2,317,072 - Pf1N1B4_2030 0.44 +0.5 2,317,264 + Pf1N1B4_2030 0.77 -0.7 2,317,264 + Pf1N1B4_2030 0.77 -0.1 2,317,264 + Pf1N1B4_2030 0.77 -2.5 2,317,264 + Pf1N1B4_2030 0.77 +0.5 2,317,264 + Pf1N1B4_2030 0.77 +0.9 2,317,264 + Pf1N1B4_2030 0.77 -0.1 2,317,265 - Pf1N1B4_2030 0.78 -0.5 2,317,265 - Pf1N1B4_2030 0.78 +1.1 2,317,265 - Pf1N1B4_2030 0.78 +0.6 2,317,275 + Pf1N1B4_2030 0.79 -2.8 2,317,275 + Pf1N1B4_2030 0.79 +0.3 2,317,275 + Pf1N1B4_2030 0.79 +0.3 2,317,275 + Pf1N1B4_2030 0.79 -1.3 2,317,275 + Pf1N1B4_2030 0.79 +0.1 2,317,275 + Pf1N1B4_2030 0.79 +1.1 2,317,276 - Pf1N1B4_2030 0.79 -0.5 2,317,276 - Pf1N1B4_2030 0.79 -0.7 2,317,276 - Pf1N1B4_2030 0.79 -0.3 2,317,276 - Pf1N1B4_2030 0.79 +0.5 2,317,276 - Pf1N1B4_2030 0.79 -0.8 2,317,393 - -0.9 2,317,406 + -1.1 2,317,407 - +1.5 2,317,448 + +0.1 2,317,448 + +0.2 2,317,449 - +0.9 2,317,449 - +0.0 2,317,518 - -0.2 2,317,518 - -2.7 2,317,579 + +3.5 2,317,579 + +1.0 2,317,579 + -2.0 2,317,579 + -2.0 2,317,579 + +0.3 2,317,580 - -1.2 2,317,580 - +1.8 2,317,580 - -0.5 2,317,580 - -1.0 2,317,580 - -1.0 2,317,580 - +1.5 2,317,580 - -1.5 2,317,742 - +2.5 2,317,772 + Pf1N1B4_2031 0.28 -0.2 2,317,772 + Pf1N1B4_2031 0.28 -0.7 2,317,772 + Pf1N1B4_2031 0.28 -1.3 2,317,772 + Pf1N1B4_2031 0.28 -0.5 2,317,772 + Pf1N1B4_2031 0.28 -0.5 2,317,772 + Pf1N1B4_2031 0.28 -0.8 2,317,773 - Pf1N1B4_2031 0.29 -2.2 2,317,773 - Pf1N1B4_2031 0.29 +0.9 2,317,773 - Pf1N1B4_2031 0.29 -1.3 2,317,773 - Pf1N1B4_2031 0.29 -0.5 2,317,850 + Pf1N1B4_2031 0.37 -1.7 2,317,850 + Pf1N1B4_2031 0.37 +0.5 2,317,850 + Pf1N1B4_2031 0.37 -0.5 2,317,850 + Pf1N1B4_2031 0.37 -0.7 2,317,850 + Pf1N1B4_2031 0.37 +0.3 2,317,850 + Pf1N1B4_2031 0.37 +0.0 2,317,850 + Pf1N1B4_2031 0.37 -2.8 2,317,851 - Pf1N1B4_2031 0.37 -1.3 2,317,851 - Pf1N1B4_2031 0.37 -0.1 2,317,851 - Pf1N1B4_2031 0.37 +0.9 2,317,851 - Pf1N1B4_2031 0.37 +0.5 2,317,879 + Pf1N1B4_2031 0.40 +0.5 2,317,879 + Pf1N1B4_2031 0.40 +0.3 2,317,879 + Pf1N1B4_2031 0.40 +0.0 2,317,880 - Pf1N1B4_2031 0.40 -0.3 2,317,880 - Pf1N1B4_2031 0.40 -1.0 2,317,880 - Pf1N1B4_2031 0.40 +2.3 2,317,880 - Pf1N1B4_2031 0.40 -0.1 2,317,880 - Pf1N1B4_2031 0.40 -0.3 2,317,880 - Pf1N1B4_2031 0.40 -1.1 2,317,880 - Pf1N1B4_2031 0.40 -0.8 2,317,907 + Pf1N1B4_2031 0.43 +1.5 2,317,907 + Pf1N1B4_2031 0.43 -0.7 2,317,907 + Pf1N1B4_2031 0.43 +2.1 2,317,907 + Pf1N1B4_2031 0.43 -0.5 2,317,907 + Pf1N1B4_2031 0.43 -0.5 2,317,907 + Pf1N1B4_2031 0.43 -1.8 2,317,908 - Pf1N1B4_2031 0.44 +1.0 2,317,908 - Pf1N1B4_2031 0.44 +0.2 2,317,908 - Pf1N1B4_2031 0.44 +1.2 2,317,908 - Pf1N1B4_2031 0.44 +2.7 2,317,971 + Pf1N1B4_2031 0.51 -0.1 2,317,972 - Pf1N1B4_2031 0.51 -1.1 2,318,304 - Pf1N1B4_2031 0.88 -1.1 2,318,489 - -1.5 2,318,900 + -1.6 2,318,942 - +1.0 2,318,942 - -1.0 2,318,942 - -1.1 2,318,942 - -1.2 2,318,942 - -2.1 2,318,956 - +1.5 2,318,966 - -0.6 2,318,966 - +1.2 2,318,966 - +0.7 2,318,966 - +0.2 2,318,966 - +0.7 2,318,966 - -0.1 2,318,966 - -0.2 2,318,992 - +1.9 2,318,992 - +1.3 2,318,992 - +2.3 2,318,992 - +0.1 2,318,992 - +0.7 2,318,992 - +1.1 2,318,992 - +0.1 2,318,992 - -1.1 2,318,992 - -0.7 2,318,992 - -0.8 2,318,992 - -2.0 2,318,994 + -0.8 2,318,994 + -2.8 2,318,995 - -1.1 2,318,995 - +2.1 2,318,995 - +0.4 2,318,995 - +2.5 2,318,995 - +1.5 2,318,995 - +0.8 2,318,995 - -1.5 2,318,995 - +1.2 2,318,995 - -0.6 2,318,995 - -0.1 2,318,995 - -1.7 2,318,995 - -0.5 2,318,995 - -0.1 2,318,995 - +1.5 2,318,995 - +0.7 2,318,995 - +0.9 2,318,995 - +0.4 2,318,995 - +0.5 2,318,995 - +0.1 2,318,995 - +1.0 2,318,995 - +1.6 2,319,086 - +0.7 2,319,101 - +0.9 2,319,109 + -2.9 2,319,110 - +0.3 2,319,110 - +1.3 2,319,110 - +0.2 2,319,110 - +0.9 2,319,110 - +0.4 2,319,110 - +0.9 2,319,110 - +0.8 2,319,110 - -0.3 2,319,110 - +0.7 2,319,112 - +0.5 2,319,165 - +1.1 2,319,165 - -0.3 2,319,175 - +0.6 2,319,175 - +0.9 2,319,175 - +0.9 2,319,175 - -0.2 2,319,175 - -0.8 2,319,175 - -0.8 2,319,175 - -0.3 2,319,175 - +1.1 2,319,175 - +1.5 2,319,175 - -0.8 2,319,254 + -0.1 2,319,255 - +1.5 2,319,255 - +2.3 2,319,255 - -0.2 2,319,255 - +1.2 2,319,256 + -1.8 2,319,256 + +0.5 2,319,256 + -0.8 2,319,256 + -0.2 2,319,257 - +0.6 2,319,257 - +0.7 2,319,257 - +0.0 2,319,257 - +0.5 2,319,257 - -1.5 2,319,257 - +0.5 2,319,257 - +2.1 2,319,257 - +1.4 2,319,257 - +1.9 2,319,257 - +1.1 2,319,257 - +0.9 2,319,257 - +1.1 2,319,257 - +0.6 2,319,339 + -0.6 2,319,339 + -1.1 2,319,339 + -1.3 2,319,340 - +0.7 2,319,340 - +1.3 2,319,340 - -0.8 2,319,340 - -0.4 2,319,340 - -0.8 2,319,340 - +2.7 2,319,340 - -1.5 2,319,340 - +1.1 2,319,341 + -0.1 2,319,341 + -1.3 2,319,341 + +0.5 2,319,341 + -1.8 2,319,341 + -1.0 2,319,341 + -0.5 2,319,341 + +0.7 2,319,341 + +0.8 2,319,341 + +0.5 2,319,342 - -0.2 2,319,342 - +0.7 2,319,342 - -0.4 2,319,342 - -1.3 2,319,342 - +0.9 2,319,342 - +2.0 2,319,342 - +0.6 2,319,342 - -0.1 2,319,342 - -0.0 2,319,342 - -0.7 2,319,342 - +1.8 2,319,342 - +0.6 2,319,342 - +0.3 2,319,342 - +0.3 2,319,342 - +0.1 2,319,385 - +0.2
Or see this region's nucleotide sequence