Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2026

Experiment: Spectinomycin 0.05 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.05 mg/ml
2,312,846	-		Pf1N1B4_2025	0.72	-2.4
2,313,166	+				-0.1
2,313,167	-				-1.3
2,313,170	-				-2.2
2,313,183	+				+0.5
2,313,292	+				-0.5
2,313,331	+				-1.3
2,313,331	+				-2.3
2,313,352	-				-0.2
2,313,393	-				+1.3
2,313,453	-				-1.1
2,313,867	+		Pf1N1B4_2026	0.49	-0.1
2,313,867	+				+0.9
2,313,867	+		Pf1N1B4_2026	0.49	-2.0
2,314,018	-		Pf1N1B4_2026	0.65	-0.7
2,314,082	+		Pf1N1B4_2026	0.72	-1.3
2,314,083	-		Pf1N1B4_2026	0.72	+1.0
2,314,088	+		Pf1N1B4_2026	0.73	-0.5
2,314,088	+		Pf1N1B4_2026	0.73	-0.8
2,314,088	+		Pf1N1B4_2026	0.73	-0.1
2,314,088	+		Pf1N1B4_2026	0.73	-1.5
2,314,089	-		Pf1N1B4_2026	0.73	-0.6
2,314,089	-		Pf1N1B4_2026	0.73	+1.1
2,314,089	-				+2.1
2,314,092	+		Pf1N1B4_2026	0.73	-1.7
2,314,092	+		Pf1N1B4_2026	0.73	-1.1
2,314,331	+				+1.5
2,314,455	+				+1.9
2,314,455	+				-1.1
2,314,455	+				-1.7
2,314,455	+				+0.9
2,314,455	+				-0.8
2,314,455	+				+0.7
2,314,455	+				+0.2
2,314,455	+				-0.1
2,314,456	-				-0.4
2,314,456	-				-0.8
2,314,484	+				+1.2
2,314,484	+				+1.5
2,314,506	-				-0.2
2,314,534	+				-0.5
2,314,535	-				-0.7
2,314,535	-				+1.1
2,314,551	+				+1.0
2,314,551	+				-0.3
2,314,551	+				-0.5
2,314,551	+				+0.5
2,314,552	-				-1.3
2,314,552	-				-1.7
2,314,553	+				-0.6
2,314,553	+				+0.9
2,314,553	+				+0.5
2,314,553	+				+0.3
2,314,553	+				+1.9
2,314,587	+				+1.5
2,314,594	+				-0.5