Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2005 and Pf1N1B4_2006 overlap by 48 nucleotides Pf1N1B4_2006 and Pf1N1B4_2007 are separated by 114 nucleotides
Pf1N1B4_2005 - ATP-dependent DNA helicase UvrD/PcrA, at 2,287,448 to 2,289,631
_2005
Pf1N1B4_2006 - hypothetical protein, at 2,289,584 to 2,289,775
_2006
Pf1N1B4_2007 - Sensory box/GGDEF family protein, at 2,289,890 to 2,292,763
_2007
Position (kb)
2289
2290 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2288.632 kb on - strand, within Pf1N1B4_2005 at 2288.725 kb on + strand, within Pf1N1B4_2005 at 2289.096 kb on + strand, within Pf1N1B4_2005 at 2289.106 kb on - strand at 2289.151 kb on + strand, within Pf1N1B4_2005 at 2289.642 kb on + strand, within Pf1N1B4_2006 at 2289.642 kb on + strand, within Pf1N1B4_2006 at 2289.643 kb on - strand, within Pf1N1B4_2006 at 2289.643 kb on - strand, within Pf1N1B4_2006 at 2289.643 kb on - strand, within Pf1N1B4_2006 at 2289.643 kb on - strand, within Pf1N1B4_2006 at 2289.765 kb on + strand at 2289.766 kb on - strand at 2289.766 kb on - strand at 2289.795 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.833 kb on + strand at 2289.834 kb on - strand at 2289.834 kb on - strand at 2289.834 kb on - strand at 2289.834 kb on - strand at 2289.868 kb on + strand at 2289.868 kb on + strand at 2289.868 kb on + strand at 2289.868 kb on + strand at 2289.869 kb on - strand at 2289.869 kb on - strand at 2289.869 kb on - strand at 2289.869 kb on - strand at 2289.869 kb on - strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.870 kb on + strand at 2289.871 kb on - strand at 2289.871 kb on - strand at 2289.871 kb on - strand at 2289.871 kb on - strand at 2289.890 kb on - strand at 2289.950 kb on + strand at 2289.951 kb on - strand at 2289.951 kb on - strand at 2289.989 kb on + strand at 2290.070 kb on + strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.071 kb on - strand at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.189 kb on + strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.190 kb on - strand, within Pf1N1B4_2007 at 2290.328 kb on + strand, within Pf1N1B4_2007 at 2290.329 kb on - strand, within Pf1N1B4_2007 at 2290.360 kb on + strand, within Pf1N1B4_2007 at 2290.360 kb on + strand, within Pf1N1B4_2007 at 2290.361 kb on - strand, within Pf1N1B4_2007 at 2290.446 kb on + strand, within Pf1N1B4_2007 at 2290.447 kb on - strand, within Pf1N1B4_2007 at 2290.447 kb on - strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.629 kb on + strand, within Pf1N1B4_2007 at 2290.630 kb on - strand, within Pf1N1B4_2007 at 2290.630 kb on - strand, within Pf1N1B4_2007
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 2,288,632 - Pf1N1B4_2005 0.54 -0.1 2,288,725 + Pf1N1B4_2005 0.58 +0.0 2,289,096 + Pf1N1B4_2005 0.75 -0.5 2,289,106 - -0.1 2,289,151 + Pf1N1B4_2005 0.78 +1.1 2,289,642 + Pf1N1B4_2006 0.30 -1.7 2,289,642 + Pf1N1B4_2006 0.30 -1.0 2,289,643 - Pf1N1B4_2006 0.31 -1.5 2,289,643 - Pf1N1B4_2006 0.31 -2.0 2,289,643 - Pf1N1B4_2006 0.31 +0.1 2,289,643 - Pf1N1B4_2006 0.31 +1.5 2,289,765 + -0.3 2,289,766 - -0.5 2,289,766 - +0.5 2,289,795 + +1.0 2,289,833 + -0.3 2,289,833 + -1.3 2,289,833 + -2.5 2,289,833 + -0.3 2,289,833 + -0.5 2,289,833 + -0.8 2,289,834 - +0.1 2,289,834 - +0.5 2,289,834 - -0.3 2,289,834 - +0.0 2,289,868 + -0.7 2,289,868 + -1.5 2,289,868 + -1.1 2,289,868 + +1.1 2,289,869 - -0.3 2,289,869 - -0.4 2,289,869 - -1.3 2,289,869 - +1.2 2,289,869 - -0.9 2,289,870 + +0.3 2,289,870 + +0.8 2,289,870 + -1.7 2,289,870 + +1.2 2,289,870 + -0.4 2,289,870 + +1.1 2,289,871 - -0.3 2,289,871 - -0.6 2,289,871 - -0.9 2,289,871 - +1.6 2,289,890 - +1.1 2,289,950 + +0.2 2,289,951 - +0.1 2,289,951 - -0.3 2,289,989 + -0.7 2,290,070 + -1.2 2,290,071 - -1.3 2,290,071 - -1.1 2,290,071 - +1.1 2,290,071 - +1.5 2,290,071 - -1.0 2,290,071 - -1.1 2,290,189 + Pf1N1B4_2007 0.10 -0.9 2,290,189 + Pf1N1B4_2007 0.10 +0.7 2,290,189 + Pf1N1B4_2007 0.10 -1.2 2,290,189 + Pf1N1B4_2007 0.10 +1.6 2,290,189 + Pf1N1B4_2007 0.10 +1.8 2,290,189 + Pf1N1B4_2007 0.10 +3.0 2,290,189 + Pf1N1B4_2007 0.10 -0.7 2,290,189 + Pf1N1B4_2007 0.10 +0.3 2,290,190 - Pf1N1B4_2007 0.10 +2.3 2,290,190 - Pf1N1B4_2007 0.10 +0.7 2,290,190 - Pf1N1B4_2007 0.10 +0.5 2,290,190 - Pf1N1B4_2007 0.10 -1.1 2,290,190 - Pf1N1B4_2007 0.10 -0.8 2,290,190 - Pf1N1B4_2007 0.10 -1.1 2,290,190 - Pf1N1B4_2007 0.10 +1.3 2,290,328 + Pf1N1B4_2007 0.15 -0.5 2,290,329 - Pf1N1B4_2007 0.15 +0.7 2,290,360 + Pf1N1B4_2007 0.16 -0.2 2,290,360 + Pf1N1B4_2007 0.16 +0.0 2,290,361 - Pf1N1B4_2007 0.16 -0.1 2,290,446 + Pf1N1B4_2007 0.19 -0.7 2,290,447 - Pf1N1B4_2007 0.19 +0.6 2,290,447 - Pf1N1B4_2007 0.19 +0.3 2,290,629 + Pf1N1B4_2007 0.26 -1.5 2,290,629 + Pf1N1B4_2007 0.26 -0.5 2,290,629 + Pf1N1B4_2007 0.26 -1.5 2,290,629 + Pf1N1B4_2007 0.26 +2.5 2,290,629 + Pf1N1B4_2007 0.26 -1.1 2,290,630 - Pf1N1B4_2007 0.26 +1.0 2,290,630 - Pf1N1B4_2007 0.26 -0.9
Or see this region's nucleotide sequence