Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_170 and Pf1N1B4_171 are separated by 332 nucleotides Pf1N1B4_171 and Pf1N1B4_172 are separated by 139 nucleotides
Pf1N1B4_170 - FIG00962118: hypothetical protein, at 211,335 to 211,589
_170
Pf1N1B4_171 - Aldehyde dehydrogenase (EC 1.2.1.3), at 211,922 to 213,355
_171
Pf1N1B4_172 - Probable alkylhalidase homolog (EC 3.8.1.1), at 213,495 to 214,790
_172
Position (kb) 211
212
213
214 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 210.938 kb on + strand at 210.939 kb on - strand at 210.960 kb on + strand at 210.961 kb on - strand at 211.024 kb on - strand at 211.058 kb on - strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.155 kb on + strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.156 kb on - strand at 211.343 kb on - strand at 211.593 kb on + strand at 211.594 kb on - strand at 211.639 kb on - strand at 211.904 kb on - strand at 212.189 kb on - strand, within Pf1N1B4_171 at 212.220 kb on + strand, within Pf1N1B4_171 at 212.220 kb on + strand, within Pf1N1B4_171 at 212.220 kb on + strand at 212.220 kb on + strand, within Pf1N1B4_171 at 212.220 kb on + strand, within Pf1N1B4_171 at 212.221 kb on - strand, within Pf1N1B4_171 at 212.221 kb on - strand, within Pf1N1B4_171 at 212.221 kb on - strand at 212.221 kb on - strand, within Pf1N1B4_171 at 212.221 kb on - strand, within Pf1N1B4_171 at 212.221 kb on - strand, within Pf1N1B4_171 at 212.246 kb on + strand, within Pf1N1B4_171 at 212.246 kb on + strand, within Pf1N1B4_171 at 212.247 kb on - strand, within Pf1N1B4_171 at 212.446 kb on + strand, within Pf1N1B4_171 at 212.641 kb on - strand, within Pf1N1B4_171 at 212.795 kb on + strand, within Pf1N1B4_171 at 212.795 kb on + strand, within Pf1N1B4_171 at 212.796 kb on - strand, within Pf1N1B4_171 at 212.796 kb on - strand, within Pf1N1B4_171 at 212.796 kb on - strand, within Pf1N1B4_171 at 212.796 kb on - strand, within Pf1N1B4_171 at 213.320 kb on + strand at 213.353 kb on + strand at 213.353 kb on + strand at 213.354 kb on - strand at 213.504 kb on - strand at 213.504 kb on - strand at 213.504 kb on - strand at 213.504 kb on - strand at 213.504 kb on - strand at 213.506 kb on - strand at 213.506 kb on - strand at 213.506 kb on - strand at 213.699 kb on + strand, within Pf1N1B4_172 at 213.820 kb on + strand, within Pf1N1B4_172 at 213.821 kb on - strand, within Pf1N1B4_172 at 213.821 kb on - strand, within Pf1N1B4_172 at 213.821 kb on - strand, within Pf1N1B4_172 at 213.918 kb on + strand, within Pf1N1B4_172 at 213.919 kb on - strand, within Pf1N1B4_172 at 213.919 kb on - strand at 214.083 kb on + strand, within Pf1N1B4_172 at 214.083 kb on + strand, within Pf1N1B4_172 at 214.084 kb on - strand, within Pf1N1B4_172
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 210,938 + +0.8 210,939 - +1.0 210,960 + -1.8 210,961 - -1.5 211,024 - -1.0 211,058 - -1.2 211,155 + +0.8 211,155 + +2.6 211,155 + +1.4 211,155 + +0.2 211,155 + +0.4 211,155 + -1.8 211,155 + +0.4 211,155 + +0.1 211,155 + +0.3 211,156 - -0.3 211,156 - -0.8 211,156 - +0.6 211,156 - +0.1 211,156 - -2.2 211,156 - -1.2 211,343 - -0.6 211,593 + +0.3 211,594 - -1.2 211,639 - +2.4 211,904 - -0.2 212,189 - Pf1N1B4_171 0.19 -1.5 212,220 + Pf1N1B4_171 0.21 -0.5 212,220 + Pf1N1B4_171 0.21 +0.5 212,220 + -0.8 212,220 + Pf1N1B4_171 0.21 +0.3 212,220 + Pf1N1B4_171 0.21 +0.1 212,221 - Pf1N1B4_171 0.21 +0.1 212,221 - Pf1N1B4_171 0.21 +0.5 212,221 - +1.8 212,221 - Pf1N1B4_171 0.21 +0.4 212,221 - Pf1N1B4_171 0.21 -0.6 212,221 - Pf1N1B4_171 0.21 +0.3 212,246 + Pf1N1B4_171 0.23 -2.4 212,246 + Pf1N1B4_171 0.23 +0.6 212,247 - Pf1N1B4_171 0.23 +1.3 212,446 + Pf1N1B4_171 0.37 -0.2 212,641 - Pf1N1B4_171 0.50 +1.8 212,795 + Pf1N1B4_171 0.61 -0.2 212,795 + Pf1N1B4_171 0.61 -0.2 212,796 - Pf1N1B4_171 0.61 -2.8 212,796 - Pf1N1B4_171 0.61 -0.0 212,796 - Pf1N1B4_171 0.61 +0.8 212,796 - Pf1N1B4_171 0.61 +1.0 213,320 + -0.9 213,353 + +0.9 213,353 + +1.0 213,354 - +0.5 213,504 - +0.6 213,504 - +0.2 213,504 - +1.2 213,504 - +0.2 213,504 - +0.7 213,506 - +0.8 213,506 - +0.0 213,506 - +0.3 213,699 + Pf1N1B4_172 0.16 +0.3 213,820 + Pf1N1B4_172 0.25 +0.3 213,821 - Pf1N1B4_172 0.25 +1.8 213,821 - Pf1N1B4_172 0.25 -0.1 213,821 - Pf1N1B4_172 0.25 +0.1 213,918 + Pf1N1B4_172 0.33 -0.2 213,919 - Pf1N1B4_172 0.33 +0.0 213,919 - +0.1 214,083 + Pf1N1B4_172 0.45 +0.8 214,083 + Pf1N1B4_172 0.45 -0.4 214,084 - Pf1N1B4_172 0.45 +2.4
Or see this region's nucleotide sequence