Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1640 and Pf1N1B4_1641 are separated by 13 nucleotides Pf1N1B4_1641 and Pf1N1B4_1642 are separated by 78 nucleotides Pf1N1B4_1642 and Pf1N1B4_1643 overlap by 4 nucleotides Pf1N1B4_1643 and Pf1N1B4_1644 are separated by 7 nucleotides
Pf1N1B4_1640 - Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1), at 1,883,799 to 1,884,572
_1640
Pf1N1B4_1641 - Lysine-arginine-ornithine-binding periplasmic protein precursor (TC 3.A.1.3.1), at 1,884,586 to 1,885,332
_1641
Pf1N1B4_1642 - Arginine ABC transporter, permease protein ArtQ, at 1,885,411 to 1,886,106
_1642
Pf1N1B4_1643 - Amino acid ABC transporter, permease protein, at 1,886,103 to 1,886,792
_1643
Pf1N1B4_1644 - SAM-dependent methyltransferases, at 1,886,800 to 1,888,002
_1644
Position (kb)
1885
1886
1887 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1884.595 kb on + strand at 1884.596 kb on - strand at 1884.596 kb on - strand at 1884.789 kb on + strand, within Pf1N1B4_1641 at 1884.789 kb on + strand, within Pf1N1B4_1641 at 1884.789 kb on + strand, within Pf1N1B4_1641 at 1884.790 kb on - strand, within Pf1N1B4_1641 at 1884.790 kb on - strand, within Pf1N1B4_1641 at 1884.904 kb on + strand, within Pf1N1B4_1641 at 1884.904 kb on + strand, within Pf1N1B4_1641 at 1884.904 kb on + strand, within Pf1N1B4_1641 at 1884.905 kb on - strand, within Pf1N1B4_1641 at 1884.905 kb on - strand, within Pf1N1B4_1641 at 1884.907 kb on + strand, within Pf1N1B4_1641 at 1884.908 kb on - strand, within Pf1N1B4_1641 at 1884.908 kb on - strand, within Pf1N1B4_1641 at 1884.908 kb on - strand, within Pf1N1B4_1641 at 1884.908 kb on - strand, within Pf1N1B4_1641 at 1885.057 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.126 kb on + strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.127 kb on - strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.135 kb on + strand, within Pf1N1B4_1641 at 1885.136 kb on - strand, within Pf1N1B4_1641 at 1885.136 kb on - strand, within Pf1N1B4_1641 at 1885.136 kb on - strand, within Pf1N1B4_1641 at 1885.136 kb on - strand, within Pf1N1B4_1641 at 1885.168 kb on + strand, within Pf1N1B4_1641 at 1885.168 kb on + strand, within Pf1N1B4_1641 at 1885.168 kb on + strand, within Pf1N1B4_1641 at 1885.169 kb on - strand, within Pf1N1B4_1641 at 1885.288 kb on + strand at 1885.288 kb on + strand at 1885.288 kb on + strand at 1885.289 kb on - strand at 1885.289 kb on - strand at 1885.289 kb on - strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.309 kb on + strand at 1885.310 kb on - strand at 1885.310 kb on - strand at 1885.310 kb on - strand at 1885.310 kb on - strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.327 kb on + strand at 1885.328 kb on - strand at 1885.328 kb on - strand at 1885.328 kb on - strand at 1885.328 kb on - strand at 1885.543 kb on + strand, within Pf1N1B4_1642 at 1885.573 kb on + strand, within Pf1N1B4_1642 at 1885.574 kb on - strand, within Pf1N1B4_1642 at 1885.624 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1885.999 kb on + strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.000 kb on - strand, within Pf1N1B4_1642 at 1886.001 kb on + strand, within Pf1N1B4_1642 at 1886.001 kb on + strand, within Pf1N1B4_1642 at 1886.002 kb on - strand, within Pf1N1B4_1642 at 1886.002 kb on - strand, within Pf1N1B4_1642 at 1886.003 kb on - strand, within Pf1N1B4_1642 at 1886.020 kb on + strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.021 kb on - strand, within Pf1N1B4_1642 at 1886.061 kb on + strand at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.245 kb on - strand, within Pf1N1B4_1643 at 1886.268 kb on + strand, within Pf1N1B4_1643 at 1886.268 kb on + strand, within Pf1N1B4_1643 at 1886.269 kb on - strand, within Pf1N1B4_1643 at 1886.269 kb on - strand, within Pf1N1B4_1643 at 1886.319 kb on + strand, within Pf1N1B4_1643 at 1886.319 kb on + strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.320 kb on - strand, within Pf1N1B4_1643 at 1886.322 kb on + strand, within Pf1N1B4_1643 at 1886.323 kb on - strand, within Pf1N1B4_1643 at 1886.323 kb on - strand, within Pf1N1B4_1643 at 1886.427 kb on + strand, within Pf1N1B4_1643 at 1886.427 kb on + strand, within Pf1N1B4_1643 at 1886.427 kb on + strand, within Pf1N1B4_1643 at 1886.428 kb on - strand at 1886.428 kb on - strand, within Pf1N1B4_1643 at 1886.428 kb on - strand, within Pf1N1B4_1643 at 1886.428 kb on - strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.526 kb on + strand, within Pf1N1B4_1643 at 1886.527 kb on - strand, within Pf1N1B4_1643 at 1886.527 kb on - strand, within Pf1N1B4_1643 at 1886.527 kb on - strand, within Pf1N1B4_1643 at 1886.568 kb on + strand, within Pf1N1B4_1643 at 1886.568 kb on + strand, within Pf1N1B4_1643 at 1886.670 kb on + strand, within Pf1N1B4_1643 at 1886.671 kb on - strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.709 kb on + strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.710 kb on - strand, within Pf1N1B4_1643 at 1886.724 kb on + strand at 1886.725 kb on - strand at 1886.980 kb on - strand, within Pf1N1B4_1644 at 1886.980 kb on - strand, within Pf1N1B4_1644 at 1886.980 kb on - strand, within Pf1N1B4_1644 at 1887.062 kb on + strand, within Pf1N1B4_1644 at 1887.062 kb on + strand at 1887.072 kb on + strand, within Pf1N1B4_1644 at 1887.073 kb on - strand, within Pf1N1B4_1644
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,884,595 + +1.4 1,884,596 - +0.1 1,884,596 - -3.5 1,884,789 + Pf1N1B4_1641 0.27 -1.4 1,884,789 + Pf1N1B4_1641 0.27 -1.2 1,884,789 + Pf1N1B4_1641 0.27 +0.2 1,884,790 - Pf1N1B4_1641 0.27 -1.3 1,884,790 - Pf1N1B4_1641 0.27 -0.2 1,884,904 + Pf1N1B4_1641 0.43 -0.2 1,884,904 + Pf1N1B4_1641 0.43 +0.3 1,884,904 + Pf1N1B4_1641 0.43 -0.4 1,884,905 - Pf1N1B4_1641 0.43 -1.3 1,884,905 - Pf1N1B4_1641 0.43 -0.2 1,884,907 + Pf1N1B4_1641 0.43 -0.2 1,884,908 - Pf1N1B4_1641 0.43 -1.3 1,884,908 - Pf1N1B4_1641 0.43 -1.4 1,884,908 - Pf1N1B4_1641 0.43 +0.1 1,884,908 - Pf1N1B4_1641 0.43 -1.1 1,885,057 + Pf1N1B4_1641 0.63 +0.6 1,885,126 + Pf1N1B4_1641 0.72 +0.3 1,885,126 + Pf1N1B4_1641 0.72 +0.2 1,885,126 + Pf1N1B4_1641 0.72 +0.6 1,885,126 + Pf1N1B4_1641 0.72 -1.2 1,885,126 + Pf1N1B4_1641 0.72 -3.1 1,885,126 + Pf1N1B4_1641 0.72 +0.4 1,885,126 + Pf1N1B4_1641 0.72 +1.0 1,885,126 + Pf1N1B4_1641 0.72 -0.8 1,885,126 + Pf1N1B4_1641 0.72 -1.2 1,885,126 + Pf1N1B4_1641 0.72 -2.1 1,885,126 + Pf1N1B4_1641 0.72 -0.5 1,885,126 + Pf1N1B4_1641 0.72 +0.8 1,885,126 + Pf1N1B4_1641 0.72 +0.4 1,885,127 - Pf1N1B4_1641 0.72 +0.4 1,885,127 - Pf1N1B4_1641 0.72 -1.1 1,885,127 - Pf1N1B4_1641 0.72 -0.2 1,885,127 - Pf1N1B4_1641 0.72 +0.1 1,885,127 - Pf1N1B4_1641 0.72 +2.2 1,885,127 - Pf1N1B4_1641 0.72 -1.8 1,885,127 - Pf1N1B4_1641 0.72 +0.8 1,885,127 - Pf1N1B4_1641 0.72 +0.2 1,885,127 - Pf1N1B4_1641 0.72 +0.2 1,885,127 - Pf1N1B4_1641 0.72 +0.9 1,885,135 + Pf1N1B4_1641 0.73 -1.2 1,885,135 + Pf1N1B4_1641 0.73 -0.5 1,885,135 + Pf1N1B4_1641 0.73 -1.5 1,885,135 + Pf1N1B4_1641 0.73 +0.7 1,885,135 + Pf1N1B4_1641 0.73 +0.0 1,885,135 + Pf1N1B4_1641 0.73 -2.1 1,885,135 + Pf1N1B4_1641 0.73 -0.9 1,885,135 + Pf1N1B4_1641 0.73 -1.1 1,885,136 - Pf1N1B4_1641 0.74 -1.8 1,885,136 - Pf1N1B4_1641 0.74 -0.9 1,885,136 - Pf1N1B4_1641 0.74 -0.7 1,885,136 - Pf1N1B4_1641 0.74 +0.3 1,885,168 + Pf1N1B4_1641 0.78 +0.0 1,885,168 + Pf1N1B4_1641 0.78 +1.1 1,885,168 + Pf1N1B4_1641 0.78 +1.4 1,885,169 - Pf1N1B4_1641 0.78 +1.7 1,885,288 + -1.1 1,885,288 + -1.8 1,885,288 + -0.8 1,885,289 - +1.4 1,885,289 - -1.8 1,885,289 - -1.2 1,885,309 + -2.3 1,885,309 + +1.4 1,885,309 + +0.5 1,885,309 + +1.4 1,885,309 + -0.6 1,885,309 + +2.2 1,885,309 + -0.3 1,885,309 + -0.2 1,885,310 - +0.8 1,885,310 - -0.5 1,885,310 - +1.1 1,885,310 - +0.1 1,885,327 + -0.5 1,885,327 + +0.2 1,885,327 + +0.6 1,885,327 + +0.0 1,885,327 + -0.8 1,885,327 + -1.4 1,885,327 + +0.8 1,885,327 + -0.9 1,885,327 + -0.9 1,885,328 - -1.6 1,885,328 - -0.9 1,885,328 - -0.6 1,885,328 - +0.4 1,885,543 + Pf1N1B4_1642 0.19 -0.0 1,885,573 + Pf1N1B4_1642 0.23 +0.8 1,885,574 - Pf1N1B4_1642 0.23 -0.2 1,885,624 + Pf1N1B4_1642 0.31 +0.4 1,885,999 + Pf1N1B4_1642 0.84 -1.0 1,885,999 + Pf1N1B4_1642 0.84 -0.9 1,885,999 + Pf1N1B4_1642 0.84 +0.7 1,885,999 + Pf1N1B4_1642 0.84 -0.9 1,885,999 + Pf1N1B4_1642 0.84 -0.8 1,885,999 + Pf1N1B4_1642 0.84 +0.6 1,885,999 + Pf1N1B4_1642 0.84 +0.4 1,885,999 + Pf1N1B4_1642 0.84 +0.0 1,886,000 - Pf1N1B4_1642 0.85 -0.6 1,886,000 - Pf1N1B4_1642 0.85 -2.1 1,886,000 - Pf1N1B4_1642 0.85 +0.8 1,886,000 - Pf1N1B4_1642 0.85 +0.4 1,886,000 - Pf1N1B4_1642 0.85 -1.6 1,886,001 + Pf1N1B4_1642 0.85 +0.4 1,886,001 + Pf1N1B4_1642 0.85 -1.1 1,886,002 - Pf1N1B4_1642 0.85 -0.7 1,886,002 - Pf1N1B4_1642 0.85 +0.2 1,886,003 - Pf1N1B4_1642 0.85 -1.4 1,886,020 + Pf1N1B4_1642 0.88 +0.4 1,886,021 - Pf1N1B4_1642 0.88 +0.1 1,886,021 - Pf1N1B4_1642 0.88 -0.3 1,886,021 - Pf1N1B4_1642 0.88 +2.1 1,886,021 - Pf1N1B4_1642 0.88 -0.3 1,886,021 - Pf1N1B4_1642 0.88 -1.0 1,886,021 - Pf1N1B4_1642 0.88 -2.1 1,886,021 - Pf1N1B4_1642 0.88 +0.6 1,886,021 - Pf1N1B4_1642 0.88 +0.1 1,886,061 + -0.1 1,886,245 - Pf1N1B4_1643 0.21 -0.2 1,886,245 - Pf1N1B4_1643 0.21 +0.4 1,886,245 - Pf1N1B4_1643 0.21 +0.8 1,886,245 - Pf1N1B4_1643 0.21 +0.5 1,886,245 - Pf1N1B4_1643 0.21 -0.7 1,886,245 - Pf1N1B4_1643 0.21 -0.2 1,886,268 + Pf1N1B4_1643 0.24 +0.8 1,886,268 + Pf1N1B4_1643 0.24 -0.2 1,886,269 - Pf1N1B4_1643 0.24 -0.1 1,886,269 - Pf1N1B4_1643 0.24 +0.4 1,886,319 + Pf1N1B4_1643 0.31 -0.5 1,886,319 + Pf1N1B4_1643 0.31 +1.1 1,886,320 - Pf1N1B4_1643 0.31 -1.8 1,886,320 - Pf1N1B4_1643 0.31 +2.4 1,886,320 - Pf1N1B4_1643 0.31 -2.8 1,886,320 - Pf1N1B4_1643 0.31 +1.4 1,886,320 - Pf1N1B4_1643 0.31 +1.4 1,886,322 + Pf1N1B4_1643 0.32 -0.2 1,886,323 - Pf1N1B4_1643 0.32 -1.4 1,886,323 - Pf1N1B4_1643 0.32 -0.9 1,886,427 + Pf1N1B4_1643 0.47 +2.1 1,886,427 + Pf1N1B4_1643 0.47 -0.4 1,886,427 + Pf1N1B4_1643 0.47 -0.8 1,886,428 - -1.4 1,886,428 - Pf1N1B4_1643 0.47 +0.8 1,886,428 - Pf1N1B4_1643 0.47 +0.9 1,886,428 - Pf1N1B4_1643 0.47 -0.2 1,886,526 + Pf1N1B4_1643 0.61 -0.5 1,886,526 + Pf1N1B4_1643 0.61 +0.8 1,886,526 + Pf1N1B4_1643 0.61 +0.4 1,886,526 + Pf1N1B4_1643 0.61 +0.1 1,886,526 + Pf1N1B4_1643 0.61 -0.3 1,886,527 - Pf1N1B4_1643 0.61 +0.4 1,886,527 - Pf1N1B4_1643 0.61 -1.4 1,886,527 - Pf1N1B4_1643 0.61 +0.4 1,886,568 + Pf1N1B4_1643 0.67 -0.9 1,886,568 + Pf1N1B4_1643 0.67 +0.0 1,886,670 + Pf1N1B4_1643 0.82 -0.6 1,886,671 - Pf1N1B4_1643 0.82 -1.0 1,886,709 + Pf1N1B4_1643 0.88 +0.2 1,886,709 + Pf1N1B4_1643 0.88 +0.3 1,886,709 + Pf1N1B4_1643 0.88 -1.3 1,886,709 + Pf1N1B4_1643 0.88 +0.0 1,886,709 + Pf1N1B4_1643 0.88 -0.9 1,886,709 + Pf1N1B4_1643 0.88 -0.8 1,886,709 + Pf1N1B4_1643 0.88 -0.4 1,886,709 + Pf1N1B4_1643 0.88 -0.5 1,886,709 + Pf1N1B4_1643 0.88 -0.6 1,886,709 + Pf1N1B4_1643 0.88 +0.8 1,886,710 - Pf1N1B4_1643 0.88 -0.2 1,886,710 - Pf1N1B4_1643 0.88 +0.6 1,886,710 - Pf1N1B4_1643 0.88 -0.4 1,886,710 - Pf1N1B4_1643 0.88 -0.1 1,886,710 - Pf1N1B4_1643 0.88 -0.5 1,886,724 + +0.7 1,886,725 - -0.2 1,886,980 - Pf1N1B4_1644 0.15 +0.4 1,886,980 - Pf1N1B4_1644 0.15 +0.8 1,886,980 - Pf1N1B4_1644 0.15 +1.4 1,887,062 + Pf1N1B4_1644 0.22 +0.2 1,887,062 + +3.0 1,887,072 + Pf1N1B4_1644 0.23 -1.6 1,887,073 - Pf1N1B4_1644 0.23 +2.0
Or see this region's nucleotide sequence