Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1525 and Pf1N1B4_1526 are separated by 35 nucleotides Pf1N1B4_1526 and Pf1N1B4_1527 are separated by 369 nucleotides
Pf1N1B4_1525 - hypothetical protein, at 1,757,191 to 1,757,448
_1525
Pf1N1B4_1526 - hypothetical protein, at 1,757,484 to 1,757,612
_1526
Pf1N1B4_1527 - Queuosine Biosynthesis QueC ATPase, at 1,757,982 to 1,758,671
_1527
Position (kb)
1757
1758
1759 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1757.049 kb on - strand at 1757.049 kb on - strand at 1757.056 kb on + strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.240 kb on + strand, within Pf1N1B4_1525 at 1757.241 kb on - strand, within Pf1N1B4_1525 at 1757.368 kb on + strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.433 kb on - strand at 1757.610 kb on + strand at 1757.610 kb on + strand at 1757.610 kb on + strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.818 kb on + strand at 1757.818 kb on + strand at 1757.820 kb on + strand at 1757.820 kb on + strand at 1757.821 kb on - strand at 1757.921 kb on + strand at 1757.922 kb on - strand at 1757.988 kb on - strand at 1758.312 kb on + strand, within Pf1N1B4_1527 at 1758.313 kb on - strand, within Pf1N1B4_1527 at 1758.440 kb on + strand, within Pf1N1B4_1527 at 1758.507 kb on + strand, within Pf1N1B4_1527 at 1758.507 kb on + strand, within Pf1N1B4_1527 at 1758.524 kb on - strand, within Pf1N1B4_1527 at 1758.536 kb on + strand, within Pf1N1B4_1527 at 1758.557 kb on + strand, within Pf1N1B4_1527 at 1758.557 kb on + strand, within Pf1N1B4_1527 at 1758.613 kb on + strand at 1758.697 kb on + strand at 1758.826 kb on - strand at 1758.840 kb on - strand at 1758.965 kb on + strand at 1759.044 kb on + strand at 1759.067 kb on + strand at 1759.068 kb on - strand at 1759.068 kb on - strand at 1759.138 kb on + strand at 1759.160 kb on - strand at 1759.212 kb on - strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.292 kb on + strand at 1759.342 kb on - strand at 1759.346 kb on - strand at 1759.346 kb on - strand at 1759.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,757,049 - -0.7 1,757,049 - -0.2 1,757,056 + -1.2 1,757,057 - +0.5 1,757,057 - -0.2 1,757,057 - +0.1 1,757,057 - -1.2 1,757,057 - -0.6 1,757,236 + Pf1N1B4_1525 0.17 -0.6 1,757,236 + Pf1N1B4_1525 0.17 -0.5 1,757,236 + Pf1N1B4_1525 0.17 +0.4 1,757,236 + Pf1N1B4_1525 0.17 +0.5 1,757,236 + Pf1N1B4_1525 0.17 +0.4 1,757,236 + Pf1N1B4_1525 0.17 -1.8 1,757,236 + Pf1N1B4_1525 0.17 -0.6 1,757,236 + Pf1N1B4_1525 0.17 +0.6 1,757,237 - Pf1N1B4_1525 0.18 -0.5 1,757,237 - Pf1N1B4_1525 0.18 -1.4 1,757,237 - Pf1N1B4_1525 0.18 -0.2 1,757,237 - Pf1N1B4_1525 0.18 +2.0 1,757,237 - Pf1N1B4_1525 0.18 +1.1 1,757,240 + Pf1N1B4_1525 0.19 -0.2 1,757,241 - Pf1N1B4_1525 0.19 +0.4 1,757,368 + Pf1N1B4_1525 0.69 -0.7 1,757,369 - Pf1N1B4_1525 0.69 +0.7 1,757,369 - Pf1N1B4_1525 0.69 +0.4 1,757,369 - Pf1N1B4_1525 0.69 +0.6 1,757,369 - Pf1N1B4_1525 0.69 -0.2 1,757,396 + Pf1N1B4_1525 0.79 -0.4 1,757,396 + Pf1N1B4_1525 0.79 -0.1 1,757,396 + Pf1N1B4_1525 0.79 -0.6 1,757,396 + Pf1N1B4_1525 0.79 +1.0 1,757,397 - Pf1N1B4_1525 0.80 +1.1 1,757,397 - Pf1N1B4_1525 0.80 +1.0 1,757,397 - Pf1N1B4_1525 0.80 +0.4 1,757,397 - Pf1N1B4_1525 0.80 -0.3 1,757,397 - Pf1N1B4_1525 0.80 -0.1 1,757,432 + -0.1 1,757,432 + +0.6 1,757,432 + -1.2 1,757,432 + +1.0 1,757,433 - -0.5 1,757,610 + -1.0 1,757,610 + +0.4 1,757,610 + -0.4 1,757,636 - -0.8 1,757,636 - -0.6 1,757,636 - -0.3 1,757,636 - +1.0 1,757,818 + +0.4 1,757,818 + +1.1 1,757,820 + -1.0 1,757,820 + +0.2 1,757,821 - +0.6 1,757,921 + -0.5 1,757,922 - +2.9 1,757,988 - +1.1 1,758,312 + Pf1N1B4_1527 0.48 +0.5 1,758,313 - Pf1N1B4_1527 0.48 +0.4 1,758,440 + Pf1N1B4_1527 0.66 -1.0 1,758,507 + Pf1N1B4_1527 0.76 -1.2 1,758,507 + Pf1N1B4_1527 0.76 -0.8 1,758,524 - Pf1N1B4_1527 0.79 +0.5 1,758,536 + Pf1N1B4_1527 0.80 -0.8 1,758,557 + Pf1N1B4_1527 0.83 -1.7 1,758,557 + Pf1N1B4_1527 0.83 -1.0 1,758,613 + -1.2 1,758,697 + -0.9 1,758,826 - +0.0 1,758,840 - +1.4 1,758,965 + +0.8 1,759,044 + -1.2 1,759,067 + -0.6 1,759,068 - -0.2 1,759,068 - +0.9 1,759,138 + +0.0 1,759,160 - -0.6 1,759,212 - +1.8 1,759,292 + +1.9 1,759,292 + +1.4 1,759,292 + +0.4 1,759,292 + -0.4 1,759,292 + +0.0 1,759,342 - +0.4 1,759,346 - -0.6 1,759,346 - +0.5 1,759,514 - -0.1
Or see this region's nucleotide sequence