Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_148 and Pf1N1B4_149 overlap by 4 nucleotides Pf1N1B4_149 and Pf1N1B4_150 are separated by 304 nucleotides
Pf1N1B4_148 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 182,592 to 188,378
_148
Pf1N1B4_149 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD, at 188,375 to 191,797
_149
Pf1N1B4_150 - SyrP-like protein, at 192,102 to 193,085
_150
Position (kb) 188
189
190
191
192 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 187.720 kb on + strand, within Pf1N1B4_148 at 188.378 kb on + strand at 188.379 kb on - strand at 188.379 kb on - strand at 188.691 kb on - strand at 188.691 kb on - strand at 188.886 kb on + strand, within Pf1N1B4_149 at 189.033 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand, within Pf1N1B4_149 at 189.180 kb on + strand at 189.530 kb on - strand, within Pf1N1B4_149 at 189.783 kb on + strand, within Pf1N1B4_149 at 189.783 kb on + strand, within Pf1N1B4_149 at 189.784 kb on - strand, within Pf1N1B4_149 at 189.813 kb on - strand, within Pf1N1B4_149 at 190.002 kb on + strand, within Pf1N1B4_149 at 190.002 kb on + strand, within Pf1N1B4_149 at 190.003 kb on - strand, within Pf1N1B4_149 at 190.003 kb on - strand, within Pf1N1B4_149 at 190.150 kb on - strand, within Pf1N1B4_149 at 190.413 kb on + strand, within Pf1N1B4_149 at 190.413 kb on + strand, within Pf1N1B4_149 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.193 kb on + strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.194 kb on - strand, within Pf1N1B4_149 at 191.584 kb on - strand at 191.584 kb on - strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.637 kb on + strand at 191.638 kb on - strand at 191.883 kb on + strand at 191.906 kb on - strand at 192.305 kb on - strand, within Pf1N1B4_150 at 192.598 kb on + strand at 192.653 kb on - strand, within Pf1N1B4_150 at 192.653 kb on - strand, within Pf1N1B4_150 at 192.712 kb on + strand, within Pf1N1B4_150 at 192.713 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150 at 192.746 kb on - strand, within Pf1N1B4_150
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 187,720 + Pf1N1B4_148 0.89 +0.6 188,378 + -0.8 188,379 - +0.3 188,379 - -1.0 188,691 - -1.0 188,691 - -1.0 188,886 + Pf1N1B4_149 0.15 -0.5 189,033 + Pf1N1B4_149 0.19 -0.2 189,180 + Pf1N1B4_149 0.24 -0.2 189,180 + Pf1N1B4_149 0.24 -0.4 189,180 + +0.8 189,530 - Pf1N1B4_149 0.34 -0.8 189,783 + Pf1N1B4_149 0.41 -0.5 189,783 + Pf1N1B4_149 0.41 +1.1 189,784 - Pf1N1B4_149 0.41 +1.3 189,813 - Pf1N1B4_149 0.42 +1.9 190,002 + Pf1N1B4_149 0.48 +2.0 190,002 + Pf1N1B4_149 0.48 +1.5 190,003 - Pf1N1B4_149 0.48 +0.8 190,003 - Pf1N1B4_149 0.48 -0.8 190,150 - Pf1N1B4_149 0.52 -1.2 190,413 + Pf1N1B4_149 0.60 +0.2 190,413 + Pf1N1B4_149 0.60 -0.6 191,193 + Pf1N1B4_149 0.82 +0.7 191,193 + Pf1N1B4_149 0.82 +1.8 191,194 - Pf1N1B4_149 0.82 +2.2 191,194 - Pf1N1B4_149 0.82 +0.9 191,584 - -0.3 191,584 - -1.2 191,637 + -1.5 191,637 + +0.8 191,637 + -0.8 191,638 - +0.1 191,883 + +0.8 191,906 - -1.0 192,305 - Pf1N1B4_150 0.21 +1.5 192,598 + +0.2 192,653 - Pf1N1B4_150 0.56 -1.2 192,653 - Pf1N1B4_150 0.56 -0.2 192,712 + Pf1N1B4_150 0.62 -0.0 192,713 - Pf1N1B4_150 0.62 +0.1 192,746 - Pf1N1B4_150 0.65 +0.6 192,746 - Pf1N1B4_150 0.65 -2.2 192,746 - Pf1N1B4_150 0.65 +3.4
Or see this region's nucleotide sequence