Experiment: Spectinomycin 0.05 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_1440 and Pf1N1B4_1441 are separated by 52 nucleotides Pf1N1B4_1441 and Pf1N1B4_1442 are separated by 85 nucleotides  
        
        Pf1N1B4_1440 - Topoisomerase IV subunit B (EC 5.99.1.-), at 1,651,531 to 1,653,435 
         
        _1440 
         
        
        Pf1N1B4_1441 - Putative esterase, FIGfam005057, at 1,653,488 to 1,654,096 
         
        _1441 
         
        
        Pf1N1B4_1442 - 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), at 1,654,182 to 1,655,021 
         
        _1442 
         Position (kb)  
1653 
 
1654 
 
1655 Strain fitness (log2 ratio)  
-4 
 
-3 
 
-2 
 
-1 
 
0 
 
1 at 1653.584 kb on - strand, within Pf1N1B4_1441 at 1653.584 kb on - strand, within Pf1N1B4_1441 at 1653.584 kb on - strand, within Pf1N1B4_1441 at 1653.584 kb on - strand, within Pf1N1B4_1441 at 1653.584 kb on - strand, within Pf1N1B4_1441 at 1653.584 kb on - strand, within Pf1N1B4_1441 at 1653.584 kb on - strand, within Pf1N1B4_1441 at 1653.584 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.628 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.678 kb on - strand, within Pf1N1B4_1441 at 1653.770 kb on - strand, within Pf1N1B4_1441 at 1653.772 kb on - strand, within Pf1N1B4_1441 at 1653.772 kb on - strand, within Pf1N1B4_1441 at 1653.772 kb on - strand, within Pf1N1B4_1441 at 1653.772 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.880 kb on - strand, within Pf1N1B4_1441 at 1653.882 kb on + strand, within Pf1N1B4_1441 at 1654.024 kb on + strand, within Pf1N1B4_1441 at 1654.024 kb on + strand, within Pf1N1B4_1441 at 1654.025 kb on - strand, within Pf1N1B4_1441 at 1654.025 kb on - strand, within Pf1N1B4_1441 at 1654.075 kb on + strand at 1654.075 kb on + strand at 1654.075 kb on + strand at 1654.076 kb on - strand at 1654.076 kb on - strand at 1654.076 kb on - strand at 1654.076 kb on - strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.077 kb on + strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.078 kb on - strand at 1654.133 kb on + strand at 1654.133 kb on + strand at 1654.133 kb on + strand at 1654.133 kb on + strand at 1654.133 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.138 kb on + strand at 1654.139 kb on - strand at 1654.139 kb on - strand at 1654.169 kb on + strand at 1654.201 kb on + strand at 1655.030 kb on + strand at 1655.030 kb on + strand at 1655.031 kb on - strand at 1655.031 kb on - strand at 1655.043 kb on + strand at 1655.043 kb on + strand at 1655.044 kb on - strand at 1655.044 kb on - strand at 1655.044 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Spectinomycin 0.05 mg/ml             remove   1,653,584   -    Pf1N1B4_1441   0.16  -0.7   1,653,584   -    Pf1N1B4_1441   0.16  +0.3   1,653,584   -    Pf1N1B4_1441   0.16  +0.3   1,653,584   -    Pf1N1B4_1441   0.16  -2.2   1,653,584   -    Pf1N1B4_1441   0.16  -1.4   1,653,584   -    Pf1N1B4_1441   0.16  -2.1   1,653,584   -    Pf1N1B4_1441   0.16  -2.7   1,653,584   -    Pf1N1B4_1441   0.16  -1.8   1,653,628   -    Pf1N1B4_1441   0.23  +0.6   1,653,628   -    Pf1N1B4_1441   0.23  -1.0   1,653,628   -    Pf1N1B4_1441   0.23  -1.4   1,653,628   -    Pf1N1B4_1441   0.23  -2.2   1,653,628   -    Pf1N1B4_1441   0.23  -2.1   1,653,628   -    Pf1N1B4_1441   0.23  -0.7   1,653,628   -    Pf1N1B4_1441   0.23  -3.2   1,653,628   -    Pf1N1B4_1441   0.23  -1.7   1,653,628   -    Pf1N1B4_1441   0.23  +0.0   1,653,628   -    Pf1N1B4_1441   0.23  -2.8   1,653,678   -    Pf1N1B4_1441   0.31  -1.0   1,653,678   -    Pf1N1B4_1441   0.31  -1.5   1,653,678   -    Pf1N1B4_1441   0.31  -2.2   1,653,678   -    Pf1N1B4_1441   0.31  -1.5   1,653,678   -    Pf1N1B4_1441   0.31  -2.1   1,653,678   -    Pf1N1B4_1441   0.31  -1.6   1,653,678   -    Pf1N1B4_1441   0.31  +0.9   1,653,678   -    Pf1N1B4_1441   0.31  -1.7   1,653,678   -    Pf1N1B4_1441   0.31  -1.0   1,653,678   -    Pf1N1B4_1441   0.31  -0.2   1,653,678   -    Pf1N1B4_1441   0.31  -1.7   1,653,678   -    Pf1N1B4_1441   0.31  -2.8   1,653,678   -    Pf1N1B4_1441   0.31  -0.9   1,653,678   -    Pf1N1B4_1441   0.31  -1.2   1,653,678   -    Pf1N1B4_1441   0.31  +0.1   1,653,678   -    Pf1N1B4_1441   0.31  -2.2   1,653,678   -    Pf1N1B4_1441   0.31  -1.4   1,653,678   -    Pf1N1B4_1441   0.31  -0.5   1,653,678   -    Pf1N1B4_1441   0.31  -4.1   1,653,678   -    Pf1N1B4_1441   0.31  -3.3   1,653,678   -    Pf1N1B4_1441   0.31  -1.6   1,653,678   -    Pf1N1B4_1441   0.31  -3.0   1,653,770   -    Pf1N1B4_1441   0.46  -0.5   1,653,772   -    Pf1N1B4_1441   0.47  -1.0   1,653,772   -    Pf1N1B4_1441   0.47  -1.2   1,653,772   -    Pf1N1B4_1441   0.47  -2.9   1,653,772   -    Pf1N1B4_1441   0.47  -2.5   1,653,880   -    Pf1N1B4_1441   0.64  -0.5   1,653,880   -    Pf1N1B4_1441   0.64  -1.2   1,653,880   -    Pf1N1B4_1441   0.64  -2.4   1,653,880   -    Pf1N1B4_1441   0.64  +0.1   1,653,880   -    Pf1N1B4_1441   0.64  -0.6   1,653,880   -    Pf1N1B4_1441   0.64  -0.4   1,653,880   -    Pf1N1B4_1441   0.64  -1.2   1,653,880   -    Pf1N1B4_1441   0.64  -1.2   1,653,880   -    Pf1N1B4_1441   0.64  -2.0   1,653,880   -    Pf1N1B4_1441   0.64  -1.2   1,653,882   +    Pf1N1B4_1441   0.65  -1.1   1,654,024   +    Pf1N1B4_1441   0.88  -1.6   1,654,024   +    Pf1N1B4_1441   0.88  -3.0   1,654,025   -    Pf1N1B4_1441   0.88  -3.4   1,654,025   -    Pf1N1B4_1441   0.88  -2.4   1,654,075   +        -0.7   1,654,075   +        +0.5   1,654,075   +        -4.0   1,654,076   -        -1.2   1,654,076   -        +0.9   1,654,076   -        -2.4   1,654,076   -        -2.3   1,654,077   +        -0.7   1,654,077   +        -1.4   1,654,077   +        -3.7   1,654,077   +        -2.1   1,654,077   +        -1.5   1,654,077   +        -1.8   1,654,077   +        -0.0   1,654,077   +        -1.4   1,654,077   +        -2.5   1,654,077   +        -1.8   1,654,077   +        -0.2   1,654,077   +        -1.7   1,654,077   +        -3.4   1,654,077   +        +0.9   1,654,077   +        -1.3   1,654,077   +        -2.6   1,654,077   +        +0.1   1,654,077   +        -1.3   1,654,077   +        -1.7   1,654,077   +        -2.7   1,654,077   +        -2.2   1,654,077   +        -0.9   1,654,077   +        -1.4   1,654,077   +        -2.5   1,654,077   +        +0.0   1,654,077   +        -0.1   1,654,077   +        -2.1   1,654,078   -        -0.7   1,654,078   -        +0.0   1,654,078   -        -2.2   1,654,078   -        -1.5   1,654,078   -        -0.5   1,654,078   -        +0.8   1,654,078   -        +0.1   1,654,078   -        -1.2   1,654,078   -        -1.5   1,654,078   -        -1.1   1,654,078   -        -1.0   1,654,078   -        +0.2   1,654,078   -        -0.0   1,654,078   -        -1.0   1,654,133   +        -1.0   1,654,133   +        +1.2   1,654,133   +        -0.1   1,654,133   +        -0.2   1,654,133   +        -0.4   1,654,138   +        -0.3   1,654,138   +        -0.9   1,654,138   +        -0.7   1,654,138   +        -0.7   1,654,138   +        -1.2   1,654,138   +        -1.5   1,654,139   -        +1.3   1,654,139   -        +0.9   1,654,169   +        -1.2   1,654,201   +        +0.8   1,655,030   +        +0.6   1,655,030   +        +0.3   1,655,031   -        -0.7   1,655,031   -        -0.2   1,655,043   +        -1.2   1,655,043   +        -0.1   1,655,044   -        +0.8   1,655,044   -        -0.1   1,655,044   -        +0.1 
 
Or see this region's nucleotide sequence