Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_142

Experiment: Spectinomycin 0.05 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Spectinomycin 0.05 mg/ml
159,384	-		Pf1N1B4_141	0.60	+2.8
159,569	+		Pf1N1B4_141	0.71	-2.8
159,570	-		Pf1N1B4_141	0.71	-0.4
159,668	+		Pf1N1B4_141	0.77	-1.1
159,669	-		Pf1N1B4_141	0.77	-1.5
159,669	-		Pf1N1B4_141	0.77	+0.1
159,669	-		Pf1N1B4_141	0.77	+0.2
159,671	+		Pf1N1B4_141	0.77	-0.2
159,672	-		Pf1N1B4_141	0.77	+0.1
159,729	-		Pf1N1B4_141	0.80	-0.9
160,068	-				+0.5
160,145	-				+1.5
160,145	-				-1.5
160,145	-				-2.4
160,145	-				+0.8
160,239	-				+0.2
160,382	+				-0.2
160,382	+				+0.5
160,382	+				+0.5
160,382	+				+2.0
160,478	+				+0.8
160,478	+				-0.4
160,479	-				+1.2
160,479	-				+0.1
160,479	-				-0.8
160,479	-				+0.8
160,851	-		Pf1N1B4_142	0.24	-0.5
160,874	+		Pf1N1B4_142	0.25	-0.8
160,874	+		Pf1N1B4_142	0.25	-0.6
160,949	+		Pf1N1B4_142	0.28	+2.1
160,949	+		Pf1N1B4_142	0.28	+0.4
160,950	-		Pf1N1B4_142	0.28	-0.8
160,989	-		Pf1N1B4_142	0.29	+0.4
160,989	-		Pf1N1B4_142	0.29	+0.0
160,989	-		Pf1N1B4_142	0.29	+0.2
161,219	+		Pf1N1B4_142	0.39	-0.1
161,220	-		Pf1N1B4_142	0.39	+0.1
161,225	+		Pf1N1B4_142	0.39	+2.4
161,255	+		Pf1N1B4_142	0.40	+0.2
161,256	-		Pf1N1B4_142	0.40	-2.2
161,288	+		Pf1N1B4_142	0.41	+1.5
161,289	-		Pf1N1B4_142	0.41	+0.8
161,315	+		Pf1N1B4_142	0.42	-2.6
161,315	+		Pf1N1B4_142	0.42	-0.1
161,315	+				-0.8
161,444	+				-0.2
161,445	-		Pf1N1B4_142	0.48	-1.0
161,532	-		Pf1N1B4_142	0.51	-1.5
161,637	-		Pf1N1B4_142	0.55	+0.8
161,637	-		Pf1N1B4_142	0.55	-1.2
161,637	-		Pf1N1B4_142	0.55	-0.2
161,723	+		Pf1N1B4_142	0.59	-0.5
161,862	-		Pf1N1B4_142	0.64	-1.8
161,905	-		Pf1N1B4_142	0.66	+1.3
161,928	-		Pf1N1B4_142	0.67	-1.2
161,928	-		Pf1N1B4_142	0.67	-1.0
162,098	+				-1.2
162,147	-		Pf1N1B4_142	0.76	+1.8
162,147	-		Pf1N1B4_142	0.76	+0.2
162,359	+				-0.8
162,359	+		Pf1N1B4_142	0.85	-0.2
162,360	-		Pf1N1B4_142	0.85	-0.5
162,360	-				-0.2
162,414	-		Pf1N1B4_142	0.87	-0.4
162,414	-		Pf1N1B4_142	0.87	+0.7
162,414	-		Pf1N1B4_142	0.87	+1.2
162,414	-		Pf1N1B4_142	0.87	-0.5
162,422	+		Pf1N1B4_142	0.87	-2.0
162,422	+		Pf1N1B4_142	0.87	-0.5
162,423	-		Pf1N1B4_142	0.87	+0.4
162,423	-		Pf1N1B4_142	0.87	-2.5
162,423	-		Pf1N1B4_142	0.87	-1.4
162,423	-				+1.8
162,569	+				-0.4
162,656	+				+0.9
162,656	+				+0.2
162,659	+				-1.2
162,659	+				+0.5
162,660	-				+2.1
162,696	-				+1.2
162,934	+				-0.4
163,032	-				+0.1
163,032	-				+1.4
163,137	+				+0.2
163,138	-				-0.6
163,144	+				+0.1
163,144	+				-1.2
163,144	+				+0.6
163,144	+				+0.7
163,243	+				-0.5
163,366	+				-0.9
163,366	+				+2.2
163,414	+				-0.2
163,414	+				-0.5
163,414	+				-0.7
163,415	-				+1.7
163,415	-				+1.8
163,496	+				+0.8
163,503	+				-2.9
163,503	+				+2.0
163,504	-				+0.1
163,504	-				+0.1
163,591	+				-1.1
163,652	+				-0.5
163,653	-				-2.4