Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1373 and Pf1N1B4_1374 are separated by 28 nucleotides Pf1N1B4_1374 and Pf1N1B4_1375 are separated by 201 nucleotides Pf1N1B4_1375 and Pf1N1B4_1376 are separated by 118 nucleotides
Pf1N1B4_1373 - Urease accessory protein UreG, at 1,571,368 to 1,571,982
_1373
Pf1N1B4_1374 - HupE-UreJ family metal transporter, at 1,572,011 to 1,572,583
_1374
Pf1N1B4_1375 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 1,572,785 to 1,573,912
_1375
Pf1N1B4_1376 - Oxidoreductase, short chain dehydrogenase/reductase family, at 1,574,031 to 1,574,798
_1376
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1571.837 kb on + strand, within Pf1N1B4_1373 at 1571.850 kb on - strand, within Pf1N1B4_1373 at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.990 kb on + strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1571.991 kb on - strand at 1572.007 kb on - strand at 1572.007 kb on - strand at 1572.226 kb on + strand, within Pf1N1B4_1374 at 1572.227 kb on - strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.437 kb on + strand, within Pf1N1B4_1374 at 1572.635 kb on + strand at 1572.635 kb on + strand at 1572.743 kb on + strand at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.992 kb on + strand, within Pf1N1B4_1375 at 1572.993 kb on - strand, within Pf1N1B4_1375 at 1572.993 kb on - strand, within Pf1N1B4_1375 at 1573.103 kb on + strand, within Pf1N1B4_1375 at 1573.103 kb on + strand, within Pf1N1B4_1375 at 1573.104 kb on - strand, within Pf1N1B4_1375 at 1573.151 kb on + strand, within Pf1N1B4_1375 at 1573.151 kb on + strand, within Pf1N1B4_1375 at 1573.152 kb on - strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.280 kb on + strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.281 kb on - strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.392 kb on + strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.393 kb on - strand, within Pf1N1B4_1375 at 1573.419 kb on + strand, within Pf1N1B4_1375 at 1573.420 kb on - strand, within Pf1N1B4_1375 at 1573.495 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.529 kb on + strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.530 kb on - strand, within Pf1N1B4_1375 at 1573.597 kb on + strand, within Pf1N1B4_1375 at 1573.709 kb on + strand, within Pf1N1B4_1375 at 1573.709 kb on + strand, within Pf1N1B4_1375 at 1573.710 kb on - strand, within Pf1N1B4_1375 at 1573.788 kb on + strand, within Pf1N1B4_1375 at 1573.789 kb on - strand, within Pf1N1B4_1375 at 1573.789 kb on - strand, within Pf1N1B4_1375 at 1573.789 kb on - strand, within Pf1N1B4_1375 at 1573.805 kb on + strand at 1573.806 kb on - strand at 1573.901 kb on + strand at 1573.902 kb on - strand at 1573.917 kb on + strand at 1573.917 kb on + strand at 1573.917 kb on + strand at 1573.918 kb on - strand at 1573.918 kb on - strand at 1573.918 kb on - strand at 1573.918 kb on - strand at 1573.921 kb on + strand at 1573.921 kb on + strand at 1573.921 kb on + strand at 1573.921 kb on + strand at 1573.921 kb on + strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.922 kb on - strand at 1573.925 kb on + strand at 1574.032 kb on + strand at 1574.032 kb on + strand at 1574.042 kb on + strand at 1574.043 kb on - strand at 1574.185 kb on + strand, within Pf1N1B4_1376 at 1574.185 kb on + strand, within Pf1N1B4_1376 at 1574.186 kb on - strand, within Pf1N1B4_1376 at 1574.186 kb on - strand, within Pf1N1B4_1376 at 1574.186 kb on - strand, within Pf1N1B4_1376 at 1574.198 kb on - strand, within Pf1N1B4_1376 at 1574.198 kb on - strand, within Pf1N1B4_1376 at 1574.344 kb on + strand, within Pf1N1B4_1376 at 1574.344 kb on + strand, within Pf1N1B4_1376 at 1574.344 kb on + strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.345 kb on - strand, within Pf1N1B4_1376 at 1574.368 kb on + strand, within Pf1N1B4_1376 at 1574.368 kb on + strand, within Pf1N1B4_1376 at 1574.368 kb on + strand, within Pf1N1B4_1376 at 1574.369 kb on - strand, within Pf1N1B4_1376 at 1574.369 kb on - strand, within Pf1N1B4_1376 at 1574.369 kb on - strand, within Pf1N1B4_1376 at 1574.452 kb on + strand, within Pf1N1B4_1376 at 1574.452 kb on + strand at 1574.452 kb on + strand, within Pf1N1B4_1376 at 1574.452 kb on + strand at 1574.452 kb on + strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.453 kb on - strand, within Pf1N1B4_1376 at 1574.780 kb on + strand at 1574.780 kb on + strand at 1574.780 kb on + strand at 1574.781 kb on - strand at 1574.781 kb on - strand at 1574.838 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,571,837 + Pf1N1B4_1373 0.76 +0.1 1,571,850 - Pf1N1B4_1373 0.78 -1.5 1,571,990 + -1.6 1,571,990 + +0.6 1,571,990 + -0.2 1,571,990 + -0.4 1,571,991 - +0.2 1,571,991 - +2.3 1,571,991 - -1.1 1,571,991 - +0.5 1,571,991 - +1.1 1,571,991 - -0.7 1,571,991 - -1.7 1,571,991 - -0.7 1,572,007 - -1.6 1,572,007 - -0.4 1,572,226 + Pf1N1B4_1374 0.38 -0.7 1,572,227 - Pf1N1B4_1374 0.38 +0.2 1,572,437 + Pf1N1B4_1374 0.74 +1.3 1,572,437 + Pf1N1B4_1374 0.74 -1.7 1,572,437 + Pf1N1B4_1374 0.74 -0.7 1,572,437 + Pf1N1B4_1374 0.74 -1.0 1,572,635 + +0.0 1,572,635 + -0.5 1,572,743 + +1.0 1,572,992 + Pf1N1B4_1375 0.18 -0.7 1,572,992 + Pf1N1B4_1375 0.18 +1.3 1,572,992 + Pf1N1B4_1375 0.18 -1.1 1,572,992 + Pf1N1B4_1375 0.18 -1.0 1,572,992 + Pf1N1B4_1375 0.18 -1.8 1,572,993 - Pf1N1B4_1375 0.18 +1.3 1,572,993 - Pf1N1B4_1375 0.18 -1.2 1,573,103 + Pf1N1B4_1375 0.28 -2.1 1,573,103 + Pf1N1B4_1375 0.28 -3.6 1,573,104 - Pf1N1B4_1375 0.28 -1.8 1,573,151 + Pf1N1B4_1375 0.32 -2.2 1,573,151 + Pf1N1B4_1375 0.32 -1.0 1,573,152 - Pf1N1B4_1375 0.33 -0.3 1,573,280 + Pf1N1B4_1375 0.44 -2.4 1,573,280 + Pf1N1B4_1375 0.44 -1.3 1,573,280 + Pf1N1B4_1375 0.44 +1.8 1,573,280 + Pf1N1B4_1375 0.44 -1.3 1,573,281 - Pf1N1B4_1375 0.44 -2.4 1,573,281 - Pf1N1B4_1375 0.44 -1.6 1,573,281 - Pf1N1B4_1375 0.44 -1.2 1,573,281 - Pf1N1B4_1375 0.44 -1.7 1,573,392 + Pf1N1B4_1375 0.54 -1.4 1,573,392 + Pf1N1B4_1375 0.54 -2.1 1,573,392 + Pf1N1B4_1375 0.54 +0.0 1,573,392 + Pf1N1B4_1375 0.54 -2.2 1,573,392 + Pf1N1B4_1375 0.54 +0.0 1,573,393 - Pf1N1B4_1375 0.54 -2.9 1,573,393 - Pf1N1B4_1375 0.54 -2.9 1,573,393 - Pf1N1B4_1375 0.54 -0.7 1,573,393 - Pf1N1B4_1375 0.54 -0.8 1,573,393 - Pf1N1B4_1375 0.54 +1.1 1,573,393 - Pf1N1B4_1375 0.54 -0.7 1,573,419 + Pf1N1B4_1375 0.56 +0.9 1,573,420 - Pf1N1B4_1375 0.56 -2.0 1,573,495 + Pf1N1B4_1375 0.63 -2.3 1,573,529 + Pf1N1B4_1375 0.66 -1.5 1,573,529 + Pf1N1B4_1375 0.66 -1.5 1,573,529 + Pf1N1B4_1375 0.66 -1.0 1,573,530 - Pf1N1B4_1375 0.66 -2.1 1,573,530 - Pf1N1B4_1375 0.66 -1.0 1,573,530 - Pf1N1B4_1375 0.66 -1.0 1,573,530 - Pf1N1B4_1375 0.66 -0.5 1,573,530 - Pf1N1B4_1375 0.66 -1.7 1,573,597 + Pf1N1B4_1375 0.72 -2.6 1,573,709 + Pf1N1B4_1375 0.82 -0.3 1,573,709 + Pf1N1B4_1375 0.82 -1.7 1,573,710 - Pf1N1B4_1375 0.82 -1.3 1,573,788 + Pf1N1B4_1375 0.89 -0.3 1,573,789 - Pf1N1B4_1375 0.89 -2.3 1,573,789 - Pf1N1B4_1375 0.89 -1.7 1,573,789 - Pf1N1B4_1375 0.89 +1.7 1,573,805 + -2.9 1,573,806 - -1.7 1,573,901 + +0.0 1,573,902 - -1.7 1,573,917 + -0.3 1,573,917 + +0.1 1,573,917 + +0.8 1,573,918 - -1.7 1,573,918 - +1.3 1,573,918 - -0.8 1,573,918 - -0.0 1,573,921 + -0.7 1,573,921 + +2.1 1,573,921 + +1.0 1,573,921 + +1.1 1,573,921 + +0.5 1,573,922 - -1.0 1,573,922 - -0.4 1,573,922 - -0.3 1,573,922 - +1.3 1,573,922 - -1.4 1,573,922 - -0.5 1,573,922 - -2.1 1,573,922 - +0.1 1,573,922 - +0.5 1,573,922 - +1.3 1,573,922 - -0.3 1,573,922 - +0.3 1,573,922 - +0.7 1,573,922 - +1.0 1,573,922 - -0.4 1,573,922 - +1.1 1,573,922 - -0.5 1,573,922 - +1.3 1,573,922 - +0.3 1,573,925 + -1.3 1,574,032 + -0.1 1,574,032 + -1.3 1,574,042 + +0.2 1,574,043 - +0.6 1,574,185 + Pf1N1B4_1376 0.20 -0.6 1,574,185 + Pf1N1B4_1376 0.20 -0.2 1,574,186 - Pf1N1B4_1376 0.20 +0.2 1,574,186 - Pf1N1B4_1376 0.20 -1.3 1,574,186 - Pf1N1B4_1376 0.20 +0.7 1,574,198 - Pf1N1B4_1376 0.22 -1.5 1,574,198 - Pf1N1B4_1376 0.22 -0.1 1,574,344 + Pf1N1B4_1376 0.41 -0.5 1,574,344 + Pf1N1B4_1376 0.41 -1.5 1,574,344 + Pf1N1B4_1376 0.41 -1.6 1,574,345 - Pf1N1B4_1376 0.41 -2.6 1,574,345 - Pf1N1B4_1376 0.41 -0.2 1,574,345 - Pf1N1B4_1376 0.41 +1.6 1,574,345 - Pf1N1B4_1376 0.41 +1.5 1,574,345 - Pf1N1B4_1376 0.41 -1.7 1,574,368 + Pf1N1B4_1376 0.44 +2.1 1,574,368 + Pf1N1B4_1376 0.44 -0.1 1,574,368 + Pf1N1B4_1376 0.44 +1.3 1,574,369 - Pf1N1B4_1376 0.44 +0.2 1,574,369 - Pf1N1B4_1376 0.44 -0.1 1,574,369 - Pf1N1B4_1376 0.44 -0.2 1,574,452 + Pf1N1B4_1376 0.55 -1.8 1,574,452 + +0.3 1,574,452 + Pf1N1B4_1376 0.55 +0.3 1,574,452 + +0.0 1,574,452 + Pf1N1B4_1376 0.55 +1.1 1,574,453 - Pf1N1B4_1376 0.55 +0.0 1,574,453 - Pf1N1B4_1376 0.55 +1.3 1,574,453 - Pf1N1B4_1376 0.55 +0.1 1,574,453 - Pf1N1B4_1376 0.55 -0.8 1,574,453 - Pf1N1B4_1376 0.55 +1.5 1,574,453 - Pf1N1B4_1376 0.55 -0.1 1,574,453 - Pf1N1B4_1376 0.55 -0.1 1,574,780 + +0.3 1,574,780 + -2.3 1,574,780 + -1.0 1,574,781 - +0.0 1,574,781 - -2.7 1,574,838 + -0.2
Or see this region's nucleotide sequence