Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1353 and Pf1N1B4_1354 are separated by 67 nucleotides Pf1N1B4_1354 and Pf1N1B4_1355 are separated by 41 nucleotides
Pf1N1B4_1353 - Urease alpha subunit (EC 3.5.1.5), at 1,547,911 to 1,549,611
_1353
Pf1N1B4_1354 - hypothetical protein, at 1,549,679 to 1,552,135
_1354
Pf1N1B4_1355 - hypothetical protein, at 1,552,177 to 1,554,810
_1355
Position (kb)
1549
1550
1551
1552
1553 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1548.718 kb on + strand, within Pf1N1B4_1353 at 1548.719 kb on - strand, within Pf1N1B4_1353 at 1548.719 kb on - strand, within Pf1N1B4_1353 at 1548.785 kb on + strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1548.786 kb on - strand, within Pf1N1B4_1353 at 1549.114 kb on + strand, within Pf1N1B4_1353 at 1549.115 kb on - strand, within Pf1N1B4_1353 at 1549.115 kb on - strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.126 kb on + strand, within Pf1N1B4_1353 at 1549.233 kb on - strand, within Pf1N1B4_1353 at 1549.495 kb on + strand at 1549.496 kb on - strand at 1549.496 kb on - strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.528 kb on + strand at 1549.529 kb on - strand at 1549.529 kb on - strand at 1549.556 kb on + strand at 1549.597 kb on + strand at 1549.597 kb on + strand at 1549.597 kb on + strand at 1549.598 kb on - strand at 1549.620 kb on + strand at 1549.620 kb on + strand at 1549.620 kb on + strand at 1549.621 kb on - strand at 1549.736 kb on - strand at 1549.739 kb on + strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.740 kb on - strand at 1549.791 kb on + strand at 1549.919 kb on + strand at 1549.919 kb on + strand at 1549.935 kb on - strand, within Pf1N1B4_1354 at 1549.935 kb on - strand, within Pf1N1B4_1354 at 1550.179 kb on - strand, within Pf1N1B4_1354 at 1550.179 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.374 kb on - strand, within Pf1N1B4_1354 at 1550.632 kb on - strand, within Pf1N1B4_1354 at 1550.751 kb on + strand, within Pf1N1B4_1354 at 1550.751 kb on + strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.752 kb on - strand, within Pf1N1B4_1354 at 1550.754 kb on - strand, within Pf1N1B4_1354 at 1550.754 kb on - strand, within Pf1N1B4_1354 at 1550.896 kb on + strand, within Pf1N1B4_1354 at 1550.896 kb on + strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.897 kb on - strand, within Pf1N1B4_1354 at 1550.958 kb on - strand, within Pf1N1B4_1354 at 1550.958 kb on - strand, within Pf1N1B4_1354 at 1551.030 kb on - strand, within Pf1N1B4_1354 at 1551.410 kb on - strand, within Pf1N1B4_1354 at 1551.616 kb on - strand, within Pf1N1B4_1354 at 1551.618 kb on - strand, within Pf1N1B4_1354 at 1551.669 kb on + strand, within Pf1N1B4_1354 at 1551.670 kb on - strand, within Pf1N1B4_1354 at 1551.712 kb on - strand, within Pf1N1B4_1354 at 1551.743 kb on + strand, within Pf1N1B4_1354 at 1551.743 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.870 kb on + strand, within Pf1N1B4_1354 at 1551.871 kb on - strand, within Pf1N1B4_1354 at 1551.871 kb on - strand, within Pf1N1B4_1354 at 1551.871 kb on - strand, within Pf1N1B4_1354 at 1551.871 kb on - strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.872 kb on + strand, within Pf1N1B4_1354 at 1551.873 kb on - strand, within Pf1N1B4_1354 at 1551.873 kb on - strand, within Pf1N1B4_1354 at 1551.873 kb on - strand, within Pf1N1B4_1354 at 1551.873 kb on - strand, within Pf1N1B4_1354 at 1551.874 kb on - strand, within Pf1N1B4_1354 at 1552.095 kb on + strand at 1552.110 kb on + strand at 1552.111 kb on - strand at 1552.111 kb on - strand at 1552.111 kb on - strand at 1552.111 kb on - strand at 1552.111 kb on - strand at 1552.112 kb on + strand at 1552.122 kb on + strand at 1552.123 kb on - strand at 1552.139 kb on + strand at 1552.139 kb on + strand at 1552.139 kb on + strand at 1552.139 kb on + strand at 1552.139 kb on + strand at 1552.140 kb on - strand at 1552.331 kb on + strand at 1552.480 kb on + strand, within Pf1N1B4_1355 at 1552.480 kb on + strand, within Pf1N1B4_1355 at 1552.481 kb on - strand, within Pf1N1B4_1355 at 1552.481 kb on - strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.521 kb on + strand, within Pf1N1B4_1355 at 1552.522 kb on - strand, within Pf1N1B4_1355 at 1552.522 kb on - strand, within Pf1N1B4_1355 at 1552.522 kb on - strand, within Pf1N1B4_1355 at 1552.642 kb on + strand, within Pf1N1B4_1355 at 1552.729 kb on - strand, within Pf1N1B4_1355 at 1552.729 kb on - strand, within Pf1N1B4_1355 at 1552.742 kb on - strand, within Pf1N1B4_1355 at 1552.788 kb on + strand, within Pf1N1B4_1355 at 1552.816 kb on + strand, within Pf1N1B4_1355 at 1552.817 kb on - strand, within Pf1N1B4_1355 at 1552.817 kb on - strand, within Pf1N1B4_1355 at 1552.955 kb on + strand, within Pf1N1B4_1355 at 1552.956 kb on - strand, within Pf1N1B4_1355 at 1552.956 kb on - strand, within Pf1N1B4_1355 at 1553.021 kb on + strand, within Pf1N1B4_1355 at 1553.120 kb on + strand, within Pf1N1B4_1355 at 1553.121 kb on - strand, within Pf1N1B4_1355
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,548,718 + Pf1N1B4_1353 0.47 +1.3 1,548,719 - Pf1N1B4_1353 0.48 +0.9 1,548,719 - Pf1N1B4_1353 0.48 +0.3 1,548,785 + Pf1N1B4_1353 0.51 +1.1 1,548,786 - Pf1N1B4_1353 0.51 -2.0 1,548,786 - Pf1N1B4_1353 0.51 +0.5 1,548,786 - Pf1N1B4_1353 0.51 +1.3 1,549,114 + Pf1N1B4_1353 0.71 +1.9 1,549,115 - Pf1N1B4_1353 0.71 +0.7 1,549,115 - Pf1N1B4_1353 0.71 +0.5 1,549,126 + Pf1N1B4_1353 0.71 -1.0 1,549,126 + Pf1N1B4_1353 0.71 +1.3 1,549,126 + Pf1N1B4_1353 0.71 +0.3 1,549,126 + Pf1N1B4_1353 0.71 -1.5 1,549,233 - Pf1N1B4_1353 0.78 +0.2 1,549,495 + -0.3 1,549,496 - +0.2 1,549,496 - +1.9 1,549,528 + +0.0 1,549,528 + -1.3 1,549,528 + -0.3 1,549,528 + +3.3 1,549,528 + +0.0 1,549,529 - +0.8 1,549,529 - -0.7 1,549,556 + +0.8 1,549,597 + +0.2 1,549,597 + -0.8 1,549,597 + +0.5 1,549,598 - +1.0 1,549,620 + -2.4 1,549,620 + +0.7 1,549,620 + -1.0 1,549,621 - -1.5 1,549,736 - +0.7 1,549,739 + -0.3 1,549,740 - -0.6 1,549,740 - -1.3 1,549,740 - -0.7 1,549,740 - -0.4 1,549,740 - -0.7 1,549,740 - +0.3 1,549,740 - +1.3 1,549,740 - +0.7 1,549,740 - -2.0 1,549,791 + +2.6 1,549,919 + -0.7 1,549,919 + -0.5 1,549,935 - Pf1N1B4_1354 0.10 +0.0 1,549,935 - Pf1N1B4_1354 0.10 -1.8 1,550,179 - Pf1N1B4_1354 0.20 -0.3 1,550,179 - Pf1N1B4_1354 0.20 +0.5 1,550,374 - Pf1N1B4_1354 0.28 -0.1 1,550,374 - Pf1N1B4_1354 0.28 -0.4 1,550,374 - Pf1N1B4_1354 0.28 -2.3 1,550,374 - Pf1N1B4_1354 0.28 +0.2 1,550,374 - Pf1N1B4_1354 0.28 +0.5 1,550,632 - Pf1N1B4_1354 0.39 +0.6 1,550,751 + Pf1N1B4_1354 0.44 +0.8 1,550,751 + Pf1N1B4_1354 0.44 +1.1 1,550,752 - Pf1N1B4_1354 0.44 +0.3 1,550,752 - Pf1N1B4_1354 0.44 -1.5 1,550,752 - Pf1N1B4_1354 0.44 +0.2 1,550,752 - Pf1N1B4_1354 0.44 +0.9 1,550,752 - Pf1N1B4_1354 0.44 +0.2 1,550,752 - Pf1N1B4_1354 0.44 +0.6 1,550,754 - Pf1N1B4_1354 0.44 +1.9 1,550,754 - Pf1N1B4_1354 0.44 +0.9 1,550,896 + Pf1N1B4_1354 0.50 +0.3 1,550,896 + Pf1N1B4_1354 0.50 -0.4 1,550,897 - Pf1N1B4_1354 0.50 +1.5 1,550,897 - Pf1N1B4_1354 0.50 -0.1 1,550,897 - Pf1N1B4_1354 0.50 -0.3 1,550,897 - Pf1N1B4_1354 0.50 +0.6 1,550,897 - Pf1N1B4_1354 0.50 +0.7 1,550,897 - Pf1N1B4_1354 0.50 +0.1 1,550,958 - Pf1N1B4_1354 0.52 +0.3 1,550,958 - Pf1N1B4_1354 0.52 +0.7 1,551,030 - Pf1N1B4_1354 0.55 +0.3 1,551,410 - Pf1N1B4_1354 0.70 -0.5 1,551,616 - Pf1N1B4_1354 0.79 +0.2 1,551,618 - Pf1N1B4_1354 0.79 -0.1 1,551,669 + Pf1N1B4_1354 0.81 -0.3 1,551,670 - Pf1N1B4_1354 0.81 +0.2 1,551,712 - Pf1N1B4_1354 0.83 -1.7 1,551,743 + Pf1N1B4_1354 0.84 -0.0 1,551,743 + Pf1N1B4_1354 0.84 -2.6 1,551,870 + Pf1N1B4_1354 0.89 -0.2 1,551,870 + Pf1N1B4_1354 0.89 +0.6 1,551,870 + Pf1N1B4_1354 0.89 +0.5 1,551,870 + Pf1N1B4_1354 0.89 +0.4 1,551,870 + Pf1N1B4_1354 0.89 +0.4 1,551,870 + Pf1N1B4_1354 0.89 -0.8 1,551,870 + Pf1N1B4_1354 0.89 -1.5 1,551,871 - Pf1N1B4_1354 0.89 -0.6 1,551,871 - Pf1N1B4_1354 0.89 -0.3 1,551,871 - Pf1N1B4_1354 0.89 -1.7 1,551,871 - Pf1N1B4_1354 0.89 -2.5 1,551,872 + Pf1N1B4_1354 0.89 +0.6 1,551,872 + Pf1N1B4_1354 0.89 +0.2 1,551,872 + Pf1N1B4_1354 0.89 -0.2 1,551,872 + Pf1N1B4_1354 0.89 -0.4 1,551,872 + Pf1N1B4_1354 0.89 +0.6 1,551,872 + Pf1N1B4_1354 0.89 -1.8 1,551,872 + Pf1N1B4_1354 0.89 -2.1 1,551,873 - Pf1N1B4_1354 0.89 +1.1 1,551,873 - Pf1N1B4_1354 0.89 -0.4 1,551,873 - Pf1N1B4_1354 0.89 +0.3 1,551,873 - Pf1N1B4_1354 0.89 +0.5 1,551,874 - Pf1N1B4_1354 0.89 -0.3 1,552,095 + -1.3 1,552,110 + -0.2 1,552,111 - -0.7 1,552,111 - -0.4 1,552,111 - -2.0 1,552,111 - +0.7 1,552,111 - +1.1 1,552,112 + -0.5 1,552,122 + -1.0 1,552,123 - +0.0 1,552,139 + -1.8 1,552,139 + +0.3 1,552,139 + +0.1 1,552,139 + +0.9 1,552,139 + -0.5 1,552,140 - -0.7 1,552,331 + +0.3 1,552,480 + Pf1N1B4_1355 0.12 +0.2 1,552,480 + Pf1N1B4_1355 0.12 +0.3 1,552,481 - Pf1N1B4_1355 0.12 +1.1 1,552,481 - Pf1N1B4_1355 0.12 +1.0 1,552,521 + Pf1N1B4_1355 0.13 -0.9 1,552,521 + Pf1N1B4_1355 0.13 +0.3 1,552,521 + Pf1N1B4_1355 0.13 -0.0 1,552,521 + Pf1N1B4_1355 0.13 -0.3 1,552,521 + Pf1N1B4_1355 0.13 -1.3 1,552,521 + Pf1N1B4_1355 0.13 +1.3 1,552,521 + Pf1N1B4_1355 0.13 -0.7 1,552,521 + Pf1N1B4_1355 0.13 +0.3 1,552,522 - Pf1N1B4_1355 0.13 +1.6 1,552,522 - Pf1N1B4_1355 0.13 +0.5 1,552,522 - Pf1N1B4_1355 0.13 -0.4 1,552,642 + Pf1N1B4_1355 0.18 -1.5 1,552,729 - Pf1N1B4_1355 0.21 +1.0 1,552,729 - Pf1N1B4_1355 0.21 -0.7 1,552,742 - Pf1N1B4_1355 0.21 -2.0 1,552,788 + Pf1N1B4_1355 0.23 +0.5 1,552,816 + Pf1N1B4_1355 0.24 +0.6 1,552,817 - Pf1N1B4_1355 0.24 +0.1 1,552,817 - Pf1N1B4_1355 0.24 +1.7 1,552,955 + Pf1N1B4_1355 0.30 -1.5 1,552,956 - Pf1N1B4_1355 0.30 +0.6 1,552,956 - Pf1N1B4_1355 0.30 -0.5 1,553,021 + Pf1N1B4_1355 0.32 -1.1 1,553,120 + Pf1N1B4_1355 0.36 +0.5 1,553,121 - Pf1N1B4_1355 0.36 -0.7
Or see this region's nucleotide sequence