Experiment: Spectinomycin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1042 and Pf1N1B4_1043 are separated by 96 nucleotides Pf1N1B4_1043 and Pf1N1B4_1044 overlap by 8 nucleotides Pf1N1B4_1044 and Pf1N1B4_1045 are separated by 194 nucleotides Pf1N1B4_1045 and Pf1N1B4_1046 are separated by 181 nucleotides
Pf1N1B4_1042 - Dihydroneopterin triphosphate epimerase, at 1,180,111 to 1,180,482
_1042
Pf1N1B4_1043 - FIG00953380: hypothetical protein, at 1,180,579 to 1,180,875
_1043
Pf1N1B4_1044 - Type III effector HopPmaJ, at 1,180,868 to 1,181,206
_1044
Pf1N1B4_1045 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 1,181,401 to 1,182,651
_1045
Pf1N1B4_1046 - Thioredoxin reductase (EC 1.8.1.9), at 1,182,833 to 1,183,789
_1046
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1180.402 kb on + strand, within Pf1N1B4_1042 at 1180.402 kb on + strand, within Pf1N1B4_1042 at 1180.507 kb on + strand at 1180.508 kb on - strand at 1180.508 kb on - strand at 1180.508 kb on - strand at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.717 kb on + strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand at 1180.718 kb on - strand, within Pf1N1B4_1043 at 1180.718 kb on - strand at 1180.719 kb on + strand, within Pf1N1B4_1043 at 1180.719 kb on + strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.720 kb on - strand, within Pf1N1B4_1043 at 1180.780 kb on + strand, within Pf1N1B4_1043 at 1180.792 kb on + strand, within Pf1N1B4_1043 at 1180.883 kb on - strand at 1180.928 kb on - strand, within Pf1N1B4_1044 at 1180.953 kb on - strand, within Pf1N1B4_1044 at 1181.279 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.285 kb on + strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.286 kb on - strand at 1181.308 kb on + strand at 1181.309 kb on - strand at 1181.309 kb on - strand at 1181.309 kb on - strand at 1181.328 kb on + strand at 1181.328 kb on + strand at 1181.329 kb on - strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.361 kb on + strand at 1181.362 kb on - strand at 1181.362 kb on - strand at 1181.366 kb on - strand at 1181.366 kb on - strand at 1181.500 kb on + strand at 1181.500 kb on + strand at 1181.500 kb on + strand at 1181.500 kb on + strand at 1181.500 kb on + strand at 1181.501 kb on - strand at 1181.630 kb on - strand, within Pf1N1B4_1045 at 1181.630 kb on - strand, within Pf1N1B4_1045 at 1181.694 kb on + strand, within Pf1N1B4_1045 at 1181.695 kb on - strand, within Pf1N1B4_1045 at 1181.836 kb on + strand, within Pf1N1B4_1045 at 1181.836 kb on + strand, within Pf1N1B4_1045 at 1181.837 kb on - strand, within Pf1N1B4_1045 at 1181.983 kb on - strand, within Pf1N1B4_1045 at 1181.983 kb on - strand, within Pf1N1B4_1045 at 1182.265 kb on + strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.266 kb on - strand, within Pf1N1B4_1045 at 1182.409 kb on + strand, within Pf1N1B4_1045 at 1182.410 kb on - strand, within Pf1N1B4_1045 at 1182.410 kb on - strand, within Pf1N1B4_1045 at 1182.654 kb on + strand at 1182.654 kb on + strand at 1182.704 kb on + strand at 1182.704 kb on + strand at 1182.705 kb on - strand at 1182.705 kb on - strand at 1182.717 kb on + strand at 1182.717 kb on + strand at 1182.733 kb on - strand at 1182.767 kb on + strand at 1182.768 kb on - strand at 1182.768 kb on - strand at 1182.786 kb on + strand at 1182.787 kb on - strand at 1182.820 kb on + strand at 1182.820 kb on + strand at 1182.821 kb on - strand at 1182.821 kb on - strand at 1182.821 kb on - strand at 1182.821 kb on - strand at 1182.821 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.05 mg/ml remove 1,180,402 + Pf1N1B4_1042 0.78 -1.1 1,180,402 + Pf1N1B4_1042 0.78 -1.8 1,180,507 + +1.8 1,180,508 - -1.9 1,180,508 - +0.1 1,180,508 - -1.8 1,180,717 + Pf1N1B4_1043 0.46 +0.0 1,180,717 + Pf1N1B4_1043 0.46 -1.6 1,180,717 + Pf1N1B4_1043 0.46 -0.7 1,180,717 + Pf1N1B4_1043 0.46 -0.6 1,180,717 + Pf1N1B4_1043 0.46 +0.7 1,180,717 + Pf1N1B4_1043 0.46 -1.5 1,180,717 + Pf1N1B4_1043 0.46 -0.7 1,180,717 + Pf1N1B4_1043 0.46 +0.8 1,180,717 + Pf1N1B4_1043 0.46 +0.2 1,180,717 + Pf1N1B4_1043 0.46 -0.1 1,180,717 + Pf1N1B4_1043 0.46 +0.2 1,180,717 + Pf1N1B4_1043 0.46 +1.7 1,180,718 - Pf1N1B4_1043 0.47 -2.3 1,180,718 - Pf1N1B4_1043 0.47 +1.7 1,180,718 - Pf1N1B4_1043 0.47 -2.6 1,180,718 - -1.1 1,180,718 - Pf1N1B4_1043 0.47 +1.0 1,180,718 - +0.2 1,180,719 + Pf1N1B4_1043 0.47 +0.1 1,180,719 + Pf1N1B4_1043 0.47 -1.1 1,180,720 - Pf1N1B4_1043 0.47 +1.6 1,180,720 - Pf1N1B4_1043 0.47 +0.2 1,180,720 - Pf1N1B4_1043 0.47 -1.0 1,180,720 - Pf1N1B4_1043 0.47 -1.9 1,180,780 + Pf1N1B4_1043 0.68 +0.2 1,180,792 + Pf1N1B4_1043 0.72 +0.9 1,180,883 - -1.1 1,180,928 - Pf1N1B4_1044 0.18 +1.0 1,180,953 - Pf1N1B4_1044 0.25 -1.5 1,181,279 + +1.6 1,181,285 + -0.5 1,181,285 + -0.0 1,181,285 + -1.1 1,181,285 + -0.2 1,181,286 - +0.8 1,181,286 - -1.4 1,181,286 - -0.7 1,181,286 - -0.4 1,181,286 - +0.9 1,181,308 + -1.4 1,181,309 - -0.2 1,181,309 - -1.4 1,181,309 - +0.1 1,181,328 + -0.4 1,181,328 + -0.4 1,181,329 - +0.5 1,181,361 + -2.0 1,181,361 + -0.6 1,181,361 + -2.0 1,181,361 + +1.9 1,181,362 - -0.0 1,181,362 - -0.2 1,181,366 - +0.5 1,181,366 - -1.0 1,181,500 + +2.5 1,181,500 + +0.2 1,181,500 + -2.3 1,181,500 + +1.2 1,181,500 + -0.6 1,181,501 - -0.4 1,181,630 - Pf1N1B4_1045 0.18 -1.1 1,181,630 - Pf1N1B4_1045 0.18 +0.7 1,181,694 + Pf1N1B4_1045 0.23 +0.3 1,181,695 - Pf1N1B4_1045 0.24 -0.2 1,181,836 + Pf1N1B4_1045 0.35 -0.1 1,181,836 + Pf1N1B4_1045 0.35 -0.9 1,181,837 - Pf1N1B4_1045 0.35 +0.3 1,181,983 - Pf1N1B4_1045 0.47 -0.6 1,181,983 - Pf1N1B4_1045 0.47 +0.6 1,182,265 + Pf1N1B4_1045 0.69 +0.8 1,182,266 - Pf1N1B4_1045 0.69 +1.6 1,182,266 - Pf1N1B4_1045 0.69 -0.1 1,182,266 - Pf1N1B4_1045 0.69 -0.6 1,182,266 - Pf1N1B4_1045 0.69 -2.6 1,182,266 - Pf1N1B4_1045 0.69 -0.7 1,182,266 - Pf1N1B4_1045 0.69 +0.3 1,182,409 + Pf1N1B4_1045 0.81 -2.0 1,182,410 - Pf1N1B4_1045 0.81 +0.4 1,182,410 - Pf1N1B4_1045 0.81 +0.2 1,182,654 + +0.5 1,182,654 + +0.0 1,182,704 + -0.8 1,182,704 + -1.8 1,182,705 - -1.1 1,182,705 - -0.4 1,182,717 + -1.6 1,182,717 + -1.3 1,182,733 - +0.3 1,182,767 + -1.1 1,182,768 - -0.1 1,182,768 - +2.6 1,182,786 + +0.2 1,182,787 - +0.9 1,182,820 + -0.1 1,182,820 + +1.8 1,182,821 - +1.5 1,182,821 - -1.1 1,182,821 - -0.3 1,182,821 - -0.8 1,182,821 - -0.2
Or see this region's nucleotide sequence