Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08635 and MMP_RS08640 are separated by 73 nucleotides MMP_RS08640 and MMP_RS08645 are separated by 3 nucleotides MMP_RS08645 and MMP_RS08650 are separated by 111 nucleotides
MMP_RS08635: MMP_RS08635 - UbiA family prenyltransferase, at 1,618,864 to 1,619,700
_RS08635
MMP_RS08640: MMP_RS08640 - TIM barrel protein, at 1,619,774 to 1,620,595
_RS08640
MMP_RS08645: MMP_RS08645 - UPF0179 family protein, at 1,620,599 to 1,621,036
_RS08645
MMP_RS08650: MMP_RS08650 - glutamine--fructose-6-phosphate transaminase (isomerizing), at 1,621,148 to 1,622,947
_RS08650
Position (kb)
1620
1621
1622 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1619.725 kb on + strand at 1619.728 kb on - strand at 1619.740 kb on - strand at 1619.851 kb on - strand at 1619.938 kb on - strand, within MMP_RS08640 at 1619.960 kb on - strand, within MMP_RS08640 at 1619.960 kb on - strand, within MMP_RS08640 at 1619.963 kb on - strand, within MMP_RS08640 at 1619.974 kb on + strand, within MMP_RS08640 at 1620.010 kb on - strand, within MMP_RS08640 at 1620.045 kb on - strand, within MMP_RS08640 at 1620.236 kb on + strand, within MMP_RS08640 at 1620.300 kb on - strand, within MMP_RS08640 at 1620.300 kb on - strand, within MMP_RS08640 at 1620.301 kb on + strand, within MMP_RS08640 at 1620.301 kb on + strand, within MMP_RS08640 at 1620.350 kb on + strand, within MMP_RS08640 at 1620.352 kb on + strand, within MMP_RS08640 at 1620.581 kb on - strand at 1620.591 kb on - strand at 1620.592 kb on + strand at 1620.656 kb on - strand, within MMP_RS08645 at 1620.656 kb on - strand, within MMP_RS08645 at 1620.671 kb on - strand, within MMP_RS08645 at 1620.672 kb on + strand, within MMP_RS08645 at 1620.672 kb on + strand, within MMP_RS08645 at 1620.672 kb on + strand, within MMP_RS08645 at 1620.716 kb on - strand, within MMP_RS08645 at 1620.716 kb on - strand, within MMP_RS08645 at 1620.723 kb on - strand, within MMP_RS08645 at 1620.738 kb on + strand, within MMP_RS08645 at 1620.773 kb on - strand, within MMP_RS08645 at 1620.794 kb on + strand, within MMP_RS08645 at 1620.978 kb on - strand, within MMP_RS08645 at 1621.036 kb on - strand at 1621.041 kb on + strand at 1621.054 kb on - strand at 1621.065 kb on + strand at 1621.068 kb on - strand at 1621.069 kb on + strand at 1621.071 kb on - strand at 1621.072 kb on + strand at 1621.072 kb on + strand at 1621.072 kb on + strand at 1621.074 kb on - strand at 1621.074 kb on - strand at 1621.075 kb on + strand at 1621.075 kb on + strand at 1621.075 kb on + strand at 1621.075 kb on + strand at 1621.078 kb on - strand at 1621.078 kb on - strand at 1621.079 kb on + strand at 1621.079 kb on + strand at 1621.081 kb on + strand at 1621.096 kb on + strand at 1621.099 kb on + strand at 1621.103 kb on + strand at 1621.103 kb on + strand at 1621.198 kb on + strand at 1621.201 kb on + strand at 1621.225 kb on - strand at 1621.761 kb on + strand, within MMP_RS08650 at 1621.827 kb on - strand, within MMP_RS08650 at 1621.828 kb on + strand, within MMP_RS08650 at 1622.035 kb on + strand, within MMP_RS08650
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE remove 1,619,725 + -0.6 1,619,728 - +0.4 1,619,740 - +0.3 1,619,851 - +0.2 1,619,938 - MMP_RS08640 0.20 -0.2 1,619,960 - MMP_RS08640 0.23 +0.4 1,619,960 - MMP_RS08640 0.23 +0.2 1,619,963 - MMP_RS08640 0.23 -0.3 1,619,974 + MMP_RS08640 0.24 +0.5 1,620,010 - MMP_RS08640 0.29 +0.8 1,620,045 - MMP_RS08640 0.33 +0.1 1,620,236 + MMP_RS08640 0.56 +0.7 1,620,300 - MMP_RS08640 0.64 +1.1 1,620,300 - MMP_RS08640 0.64 +0.9 1,620,301 + MMP_RS08640 0.64 +0.7 1,620,301 + MMP_RS08640 0.64 -0.5 1,620,350 + MMP_RS08640 0.70 +0.8 1,620,352 + MMP_RS08640 0.70 +0.3 1,620,581 - +0.5 1,620,591 - +0.3 1,620,592 + +0.5 1,620,656 - MMP_RS08645 0.13 +0.5 1,620,656 - MMP_RS08645 0.13 -1.2 1,620,671 - MMP_RS08645 0.16 -0.5 1,620,672 + MMP_RS08645 0.17 -0.8 1,620,672 + MMP_RS08645 0.17 -1.3 1,620,672 + MMP_RS08645 0.17 -2.2 1,620,716 - MMP_RS08645 0.27 -1.4 1,620,716 - MMP_RS08645 0.27 -1.8 1,620,723 - MMP_RS08645 0.28 -0.9 1,620,738 + MMP_RS08645 0.32 -1.3 1,620,773 - MMP_RS08645 0.40 -1.4 1,620,794 + MMP_RS08645 0.45 -1.0 1,620,978 - MMP_RS08645 0.87 -2.5 1,621,036 - -1.8 1,621,041 + -0.6 1,621,054 - -0.2 1,621,065 + -0.4 1,621,068 - +0.1 1,621,069 + -1.5 1,621,071 - -0.3 1,621,072 + -0.5 1,621,072 + -0.2 1,621,072 + +1.1 1,621,074 - +1.6 1,621,074 - -0.9 1,621,075 + -1.4 1,621,075 + -1.1 1,621,075 + -1.6 1,621,075 + -1.0 1,621,078 - -1.9 1,621,078 - -1.2 1,621,079 + +0.3 1,621,079 + -2.0 1,621,081 + -0.5 1,621,096 + -3.6 1,621,099 + -2.0 1,621,103 + -3.9 1,621,103 + -3.9 1,621,198 + -1.4 1,621,201 + -1.6 1,621,225 - -1.9 1,621,761 + MMP_RS08650 0.34 -1.6 1,621,827 - MMP_RS08650 0.38 -2.1 1,621,828 + MMP_RS08650 0.38 -3.5 1,622,035 + MMP_RS08650 0.49 -1.7
Or see this region's nucleotide sequence