Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2990 and Rru_A2991 overlap by 4 nucleotides Rru_A2991 and Rru_A2992 are separated by 252 nucleotides Rru_A2992 and Rru_A2993 are separated by 15 nucleotides
Rru_A2990: Rru_A2990 - precorrin-2 C20-methyltransferase (NCBI), at 3,442,544 to 3,443,278
_A2990
Rru_A2991: Rru_A2991 - Precorrin-8X methylmutase (NCBI), at 3,443,275 to 3,443,901
_A2991
Rru_A2992: Rru_A2992 - Precorrin-6Y C5,15-methyltransferase (decarboxylating) (NCBI), at 3,444,154 to 3,445,380
_A2992
Rru_A2993: Rru_A2993 - hypothetical protein (NCBI), at 3,445,396 to 3,446,841
_A2993
Position (kb)
3444
3445
3446 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3443.167 kb on + strand, within Rru_A2990 at 3443.168 kb on - strand, within Rru_A2990 at 3443.169 kb on + strand, within Rru_A2990 at 3443.170 kb on - strand, within Rru_A2990 at 3443.170 kb on - strand, within Rru_A2990 at 3443.170 kb on - strand, within Rru_A2990 at 3443.170 kb on - strand, within Rru_A2990 at 3443.360 kb on - strand, within Rru_A2991 at 3443.360 kb on - strand, within Rru_A2991 at 3443.871 kb on + strand at 3443.871 kb on + strand at 3443.871 kb on + strand at 3443.872 kb on - strand at 3443.891 kb on + strand at 3443.892 kb on - strand at 3443.961 kb on + strand at 3443.961 kb on + strand at 3443.961 kb on + strand at 3443.961 kb on + strand at 3443.966 kb on + strand at 3443.968 kb on + strand at 3443.968 kb on + strand at 3443.968 kb on + strand at 3444.109 kb on + strand at 3444.110 kb on - strand at 3444.334 kb on + strand, within Rru_A2992 at 3444.334 kb on + strand, within Rru_A2992 at 3444.335 kb on - strand, within Rru_A2992 at 3444.335 kb on - strand, within Rru_A2992 at 3444.335 kb on - strand, within Rru_A2992 at 3444.401 kb on - strand, within Rru_A2992 at 3444.401 kb on - strand, within Rru_A2992 at 3445.039 kb on - strand, within Rru_A2992 at 3445.039 kb on - strand, within Rru_A2992 at 3445.262 kb on + strand at 3445.263 kb on - strand at 3445.263 kb on - strand at 3445.352 kb on - strand at 3445.352 kb on - strand at 3445.352 kb on - strand at 3445.395 kb on - strand at 3445.395 kb on - strand at 3445.395 kb on - strand at 3445.395 kb on - strand at 3445.395 kb on - strand at 3445.395 kb on - strand at 3445.395 kb on - strand at 3445.397 kb on + strand at 3445.398 kb on - strand at 3445.398 kb on - strand at 3445.402 kb on - strand at 3445.402 kb on - strand at 3445.416 kb on + strand at 3445.442 kb on - strand at 3445.473 kb on + strand at 3445.473 kb on + strand at 3445.474 kb on - strand at 3445.474 kb on - strand at 3445.476 kb on + strand at 3445.476 kb on + strand at 3445.476 kb on + strand at 3445.476 kb on + strand at 3445.477 kb on - strand at 3445.627 kb on + strand, within Rru_A2993 at 3445.627 kb on + strand, within Rru_A2993 at 3445.628 kb on - strand, within Rru_A2993 at 3445.628 kb on - strand, within Rru_A2993 at 3445.684 kb on - strand, within Rru_A2993 at 3445.695 kb on - strand, within Rru_A2993 at 3445.720 kb on + strand, within Rru_A2993 at 3445.760 kb on + strand, within Rru_A2993 at 3445.761 kb on - strand, within Rru_A2993 at 3445.773 kb on - strand, within Rru_A2993 at 3445.798 kb on - strand, within Rru_A2993 at 3445.889 kb on + strand, within Rru_A2993 at 3445.889 kb on + strand, within Rru_A2993 at 3445.889 kb on + strand, within Rru_A2993 at 3445.890 kb on - strand, within Rru_A2993 at 3446.029 kb on + strand, within Rru_A2993 at 3446.037 kb on + strand, within Rru_A2993 at 3446.050 kb on - strand, within Rru_A2993 at 3446.080 kb on - strand, within Rru_A2993 at 3446.324 kb on - strand, within Rru_A2993
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 3,443,167 + Rru_A2990 0.85 -1.8 3,443,168 - Rru_A2990 0.85 -3.9 3,443,169 + Rru_A2990 0.85 -4.0 3,443,170 - Rru_A2990 0.85 -0.4 3,443,170 - Rru_A2990 0.85 -0.6 3,443,170 - Rru_A2990 0.85 -4.6 3,443,170 - Rru_A2990 0.85 -4.0 3,443,360 - Rru_A2991 0.14 -2.5 3,443,360 - Rru_A2991 0.14 -2.0 3,443,871 + -1.7 3,443,871 + -2.0 3,443,871 + -2.7 3,443,872 - -2.2 3,443,891 + -0.7 3,443,892 - -3.1 3,443,961 + +0.6 3,443,961 + -0.9 3,443,961 + +1.2 3,443,961 + -0.7 3,443,966 + +0.1 3,443,968 + +0.4 3,443,968 + -1.9 3,443,968 + +0.2 3,444,109 + -0.7 3,444,110 - +1.0 3,444,334 + Rru_A2992 0.15 -0.0 3,444,334 + Rru_A2992 0.15 -2.0 3,444,335 - Rru_A2992 0.15 -2.9 3,444,335 - Rru_A2992 0.15 -2.3 3,444,335 - Rru_A2992 0.15 -4.4 3,444,401 - Rru_A2992 0.20 -2.7 3,444,401 - Rru_A2992 0.20 -1.9 3,445,039 - Rru_A2992 0.72 -0.2 3,445,039 - Rru_A2992 0.72 -3.0 3,445,262 + -3.2 3,445,263 - -1.7 3,445,263 - -2.9 3,445,352 - -3.6 3,445,352 - -0.7 3,445,352 - -3.0 3,445,395 - -1.2 3,445,395 - +0.2 3,445,395 - +0.4 3,445,395 - +0.5 3,445,395 - -0.0 3,445,395 - -0.1 3,445,395 - +0.9 3,445,397 + -0.0 3,445,398 - +0.1 3,445,398 - +1.7 3,445,402 - +0.6 3,445,402 - +0.6 3,445,416 + +0.3 3,445,442 - -0.6 3,445,473 + +0.4 3,445,473 + +0.1 3,445,474 - +0.2 3,445,474 - -0.3 3,445,476 + +0.3 3,445,476 + +1.2 3,445,476 + +0.9 3,445,476 + -0.8 3,445,477 - -0.1 3,445,627 + Rru_A2993 0.16 +1.0 3,445,627 + Rru_A2993 0.16 -0.6 3,445,628 - Rru_A2993 0.16 -0.1 3,445,628 - Rru_A2993 0.16 +1.3 3,445,684 - Rru_A2993 0.20 +1.1 3,445,695 - Rru_A2993 0.21 -0.0 3,445,720 + Rru_A2993 0.22 +0.2 3,445,760 + Rru_A2993 0.25 +0.1 3,445,761 - Rru_A2993 0.25 -0.4 3,445,773 - Rru_A2993 0.26 -0.1 3,445,798 - Rru_A2993 0.28 -0.1 3,445,889 + Rru_A2993 0.34 -0.7 3,445,889 + Rru_A2993 0.34 -0.3 3,445,889 + Rru_A2993 0.34 -1.0 3,445,890 - Rru_A2993 0.34 +0.8 3,446,029 + Rru_A2993 0.44 +0.4 3,446,037 + Rru_A2993 0.44 -0.7 3,446,050 - Rru_A2993 0.45 -0.7 3,446,080 - Rru_A2993 0.47 -0.5 3,446,324 - Rru_A2993 0.64 -0.3
Or see this region's nucleotide sequence