Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2702 and Rru_AR0049 are separated by 93 nucleotides Rru_AR0049 and Rru_AR0050 are separated by 27 nucleotides Rru_AR0050 and Rru_A2703 are separated by 37 nucleotides Rru_A2703 and Rru_AR0051 are separated by 57 nucleotides Rru_AR0051 and Rru_A2704 are separated by 127 nucleotides Rru_A2704 and Rru_A2705 are separated by 274 nucleotides
Rru_A2702: Rru_A2702 - Translation elongation factor Tu (NCBI), at 3,125,604 to 3,126,794
_A2702
Rru_AR0049: Rru_AR0049 - tRNA-Gly (NCBI), at 3,126,888 to 3,126,961
_AR0049
Rru_AR0050: Rru_AR0050 - tRNA-Tyr (NCBI), at 3,126,989 to 3,127,074
_AR0050
Rru_A2703: Rru_A2703 - RNA methyltransferase TrmH, group 3 (NCBI), at 3,127,112 to 3,128,158
_A2703
Rru_AR0051: Rru_AR0051 - tRNA-Thr (NCBI), at 3,128,216 to 3,128,291
_AR0051
Rru_A2704: Rru_A2704 - hypothetical protein (NCBI), at 3,128,419 to 3,128,652
_A2704
Rru_A2705: Rru_A2705 - hypothetical protein (NCBI), at 3,128,927 to 3,129,172
_A2705
Position (kb)
3127
3128
3129 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3126.814 kb on - strand at 3126.870 kb on - strand at 3126.870 kb on - strand at 3127.741 kb on + strand, within Rru_A2703 at 3127.742 kb on - strand, within Rru_A2703 at 3127.906 kb on + strand, within Rru_A2703 at 3127.906 kb on + strand, within Rru_A2703 at 3127.906 kb on + strand, within Rru_A2703 at 3127.907 kb on - strand, within Rru_A2703 at 3127.907 kb on - strand, within Rru_A2703 at 3127.907 kb on - strand, within Rru_A2703 at 3127.907 kb on - strand, within Rru_A2703 at 3127.907 kb on - strand, within Rru_A2703 at 3128.126 kb on + strand at 3128.126 kb on + strand at 3128.126 kb on + strand at 3128.126 kb on + strand at 3128.127 kb on - strand at 3128.127 kb on - strand at 3128.127 kb on - strand at 3128.127 kb on - strand at 3128.294 kb on + strand at 3128.295 kb on - strand at 3128.334 kb on + strand at 3128.335 kb on - strand at 3128.335 kb on - strand at 3128.335 kb on - strand at 3128.396 kb on + strand at 3128.418 kb on + strand at 3128.418 kb on + strand at 3128.418 kb on + strand at 3128.418 kb on + strand at 3128.418 kb on + strand at 3128.466 kb on + strand, within Rru_A2704 at 3128.466 kb on + strand, within Rru_A2704 at 3128.466 kb on + strand, within Rru_A2704 at 3128.466 kb on + strand, within Rru_A2704 at 3128.466 kb on + strand, within Rru_A2704 at 3128.466 kb on + strand, within Rru_A2704 at 3128.467 kb on - strand, within Rru_A2704 at 3128.467 kb on - strand, within Rru_A2704 at 3128.512 kb on - strand, within Rru_A2704 at 3128.527 kb on + strand, within Rru_A2704 at 3128.527 kb on + strand, within Rru_A2704 at 3128.528 kb on - strand, within Rru_A2704 at 3128.528 kb on - strand, within Rru_A2704 at 3128.528 kb on - strand, within Rru_A2704 at 3128.528 kb on - strand, within Rru_A2704 at 3128.552 kb on + strand, within Rru_A2704 at 3128.552 kb on + strand, within Rru_A2704 at 3128.552 kb on + strand, within Rru_A2704 at 3128.552 kb on + strand, within Rru_A2704 at 3128.553 kb on - strand, within Rru_A2704 at 3128.585 kb on + strand, within Rru_A2704 at 3128.585 kb on + strand, within Rru_A2704 at 3128.585 kb on + strand, within Rru_A2704 at 3128.586 kb on - strand, within Rru_A2704 at 3128.586 kb on - strand, within Rru_A2704 at 3128.765 kb on + strand at 3128.765 kb on + strand at 3128.766 kb on - strand at 3128.799 kb on - strand at 3128.870 kb on + strand at 3128.870 kb on + strand at 3128.957 kb on + strand, within Rru_A2705 at 3128.957 kb on + strand, within Rru_A2705 at 3129.095 kb on - strand, within Rru_A2705
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 3,126,814 - -0.2 3,126,870 - +1.9 3,126,870 - -1.1 3,127,741 + Rru_A2703 0.60 -0.4 3,127,742 - Rru_A2703 0.60 -0.8 3,127,906 + Rru_A2703 0.76 -1.7 3,127,906 + Rru_A2703 0.76 -2.1 3,127,906 + Rru_A2703 0.76 -3.9 3,127,907 - Rru_A2703 0.76 -1.4 3,127,907 - Rru_A2703 0.76 +0.2 3,127,907 - Rru_A2703 0.76 -1.3 3,127,907 - Rru_A2703 0.76 +2.5 3,127,907 - Rru_A2703 0.76 +0.2 3,128,126 + -2.3 3,128,126 + -0.3 3,128,126 + -0.9 3,128,126 + -0.8 3,128,127 - -0.5 3,128,127 - -0.1 3,128,127 - -2.3 3,128,127 - -0.2 3,128,294 + +1.5 3,128,295 - -1.4 3,128,334 + -1.4 3,128,335 - -0.1 3,128,335 - +1.2 3,128,335 - -0.7 3,128,396 + +0.1 3,128,418 + -1.0 3,128,418 + +0.3 3,128,418 + -0.5 3,128,418 + +0.3 3,128,418 + +1.1 3,128,466 + Rru_A2704 0.20 -0.9 3,128,466 + Rru_A2704 0.20 -2.2 3,128,466 + Rru_A2704 0.20 +0.3 3,128,466 + Rru_A2704 0.20 +0.0 3,128,466 + Rru_A2704 0.20 +0.4 3,128,466 + Rru_A2704 0.20 +0.1 3,128,467 - Rru_A2704 0.21 +0.4 3,128,467 - Rru_A2704 0.21 +0.1 3,128,512 - Rru_A2704 0.40 +0.5 3,128,527 + Rru_A2704 0.46 -1.3 3,128,527 + Rru_A2704 0.46 -0.5 3,128,528 - Rru_A2704 0.47 -0.7 3,128,528 - Rru_A2704 0.47 -0.2 3,128,528 - Rru_A2704 0.47 -3.1 3,128,528 - Rru_A2704 0.47 -0.4 3,128,552 + Rru_A2704 0.57 -0.6 3,128,552 + Rru_A2704 0.57 +0.1 3,128,552 + Rru_A2704 0.57 -0.4 3,128,552 + Rru_A2704 0.57 +0.3 3,128,553 - Rru_A2704 0.57 -1.9 3,128,585 + Rru_A2704 0.71 +0.8 3,128,585 + Rru_A2704 0.71 -1.7 3,128,585 + Rru_A2704 0.71 -0.0 3,128,586 - Rru_A2704 0.71 +0.1 3,128,586 - Rru_A2704 0.71 -1.6 3,128,765 + +0.2 3,128,765 + -0.1 3,128,766 - -0.9 3,128,799 - +0.2 3,128,870 + +0.1 3,128,870 + +0.3 3,128,957 + Rru_A2705 0.12 -0.4 3,128,957 + Rru_A2705 0.12 -0.7 3,129,095 - Rru_A2705 0.68 -1.7
Or see this region's nucleotide sequence