Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1981 and Rru_A1982 are separated by 58 nucleotides Rru_A1982 and Rru_A1983 are separated by 105 nucleotides Rru_A1983 and Rru_A1984 are separated by 211 nucleotides Rru_A1984 and Rru_A1985 overlap by 4 nucleotides
Rru_A1981: Rru_A1981 - Succinylglutamate desuccinylase/aspartoacylase (NCBI), at 2,292,697 to 2,293,830
_A1981
Rru_A1982: Rru_A1982 - SEC-C domain protein (NCBI), at 2,293,889 to 2,294,389
_A1982
Rru_A1983: Rru_A1983 - hypothetical protein (NCBI), at 2,294,495 to 2,294,701
_A1983
Rru_A1984: Rru_A1984 - nitrogen fixation-related protein (NCBI), at 2,294,913 to 2,295,272
_A1984
Rru_A1985: Rru_A1985 - hypothetical protein (NCBI), at 2,295,269 to 2,295,700
_A1985
Position (kb)
2293
2294
2295 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2292.923 kb on + strand, within Rru_A1981 at 2292.923 kb on + strand, within Rru_A1981 at 2292.923 kb on + strand, within Rru_A1981 at 2292.924 kb on - strand, within Rru_A1981 at 2292.924 kb on - strand, within Rru_A1981 at 2293.292 kb on + strand, within Rru_A1981 at 2293.293 kb on - strand, within Rru_A1981 at 2293.293 kb on - strand, within Rru_A1981 at 2293.293 kb on - strand, within Rru_A1981 at 2293.506 kb on - strand, within Rru_A1981 at 2293.506 kb on - strand, within Rru_A1981 at 2293.727 kb on + strand at 2293.727 kb on + strand at 2293.728 kb on - strand at 2293.830 kb on + strand at 2293.830 kb on + strand at 2293.831 kb on - strand at 2293.831 kb on - strand at 2293.887 kb on + strand at 2293.890 kb on + strand at 2293.891 kb on - strand at 2293.917 kb on + strand at 2293.917 kb on + strand at 2293.917 kb on + strand at 2293.918 kb on - strand at 2293.918 kb on - strand at 2294.271 kb on + strand, within Rru_A1982 at 2294.272 kb on - strand, within Rru_A1982 at 2294.272 kb on - strand, within Rru_A1982 at 2294.392 kb on + strand at 2294.397 kb on + strand at 2294.397 kb on + strand at 2294.397 kb on + strand at 2294.397 kb on + strand at 2294.398 kb on - strand at 2294.398 kb on - strand at 2294.472 kb on + strand at 2294.472 kb on + strand at 2294.473 kb on - strand at 2294.532 kb on + strand, within Rru_A1983 at 2294.532 kb on + strand, within Rru_A1983 at 2294.533 kb on - strand, within Rru_A1983 at 2294.533 kb on - strand, within Rru_A1983 at 2294.533 kb on - strand, within Rru_A1983 at 2294.667 kb on + strand, within Rru_A1983 at 2294.668 kb on - strand, within Rru_A1983 at 2294.668 kb on - strand, within Rru_A1983 at 2294.668 kb on - strand, within Rru_A1983 at 2294.668 kb on - strand, within Rru_A1983 at 2294.727 kb on + strand at 2294.727 kb on + strand at 2294.727 kb on + strand at 2294.728 kb on - strand at 2294.728 kb on - strand at 2294.728 kb on - strand at 2295.090 kb on + strand, within Rru_A1984 at 2295.090 kb on + strand, within Rru_A1984 at 2295.091 kb on - strand, within Rru_A1984 at 2295.091 kb on - strand, within Rru_A1984 at 2295.372 kb on - strand, within Rru_A1985 at 2295.372 kb on - strand, within Rru_A1985
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 2,292,923 + Rru_A1981 0.20 -0.1 2,292,923 + Rru_A1981 0.20 -0.4 2,292,923 + Rru_A1981 0.20 +0.3 2,292,924 - Rru_A1981 0.20 -1.0 2,292,924 - Rru_A1981 0.20 -1.3 2,293,292 + Rru_A1981 0.52 -0.4 2,293,293 - Rru_A1981 0.53 +0.3 2,293,293 - Rru_A1981 0.53 +0.6 2,293,293 - Rru_A1981 0.53 +2.1 2,293,506 - Rru_A1981 0.71 -0.6 2,293,506 - Rru_A1981 0.71 -1.1 2,293,727 + -0.6 2,293,727 + -1.8 2,293,728 - +0.1 2,293,830 + -0.0 2,293,830 + +0.5 2,293,831 - -0.9 2,293,831 - -1.2 2,293,887 + +0.2 2,293,890 + +0.3 2,293,891 - +0.1 2,293,917 + +1.1 2,293,917 + -1.6 2,293,917 + +0.0 2,293,918 - -2.1 2,293,918 - -0.4 2,294,271 + Rru_A1982 0.76 -0.0 2,294,272 - Rru_A1982 0.76 +0.3 2,294,272 - Rru_A1982 0.76 +1.0 2,294,392 + +1.3 2,294,397 + +0.2 2,294,397 + -0.2 2,294,397 + +0.0 2,294,397 + +1.1 2,294,398 - +0.6 2,294,398 - -1.1 2,294,472 + +0.6 2,294,472 + -0.7 2,294,473 - +0.0 2,294,532 + Rru_A1983 0.18 +2.0 2,294,532 + Rru_A1983 0.18 +0.6 2,294,533 - Rru_A1983 0.18 +1.3 2,294,533 - Rru_A1983 0.18 +0.1 2,294,533 - Rru_A1983 0.18 +0.8 2,294,667 + Rru_A1983 0.83 -0.1 2,294,668 - Rru_A1983 0.84 -0.4 2,294,668 - Rru_A1983 0.84 -1.1 2,294,668 - Rru_A1983 0.84 +0.0 2,294,668 - Rru_A1983 0.84 -0.5 2,294,727 + +0.9 2,294,727 + -1.1 2,294,727 + -0.4 2,294,728 - +0.1 2,294,728 - -1.7 2,294,728 - -0.5 2,295,090 + Rru_A1984 0.49 -0.2 2,295,090 + Rru_A1984 0.49 -0.7 2,295,091 - Rru_A1984 0.49 -0.3 2,295,091 - Rru_A1984 0.49 +1.2 2,295,372 - Rru_A1985 0.24 -0.3 2,295,372 - Rru_A1985 0.24 -0.8
Or see this region's nucleotide sequence