Experiment: Valeric 25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1474 and Rru_A1475 are separated by 58 nucleotides Rru_A1475 and Rru_A1476 are separated by 370 nucleotides Rru_A1476 and Rru_A1477 are separated by 233 nucleotides
Rru_A1474: Rru_A1474 - Protein of unknown function DUF1446 (NCBI), at 1,739,321 to 1,740,658
_A1474
Rru_A1475: Rru_A1475 - Citrate transporter (NCBI), at 1,740,717 to 1,742,018
_A1475
Rru_A1476: Rru_A1476 - PhnA protein (NCBI), at 1,742,389 to 1,742,739
_A1476
Rru_A1477: Rru_A1477 - hypothetical protein (NCBI), at 1,742,973 to 1,743,713
_A1477
Position (kb)
1740
1741
1742
1743 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1739.841 kb on + strand, within Rru_A1474 at 1739.842 kb on - strand, within Rru_A1474 at 1739.865 kb on + strand, within Rru_A1474 at 1739.866 kb on - strand, within Rru_A1474 at 1740.018 kb on + strand, within Rru_A1474 at 1740.019 kb on - strand, within Rru_A1474 at 1740.216 kb on + strand, within Rru_A1474 at 1740.216 kb on + strand, within Rru_A1474 at 1740.489 kb on + strand, within Rru_A1474 at 1740.489 kb on + strand, within Rru_A1474 at 1740.489 kb on + strand, within Rru_A1474 at 1740.490 kb on - strand, within Rru_A1474 at 1740.490 kb on - strand, within Rru_A1474 at 1740.624 kb on + strand at 1740.658 kb on + strand at 1740.658 kb on + strand at 1740.659 kb on - strand at 1740.665 kb on + strand at 1740.686 kb on + strand at 1740.718 kb on + strand at 1740.718 kb on + strand at 1740.718 kb on + strand at 1740.719 kb on - strand at 1740.719 kb on - strand at 1740.719 kb on - strand at 1740.781 kb on + strand at 1740.781 kb on + strand at 1740.782 kb on - strand at 1740.973 kb on + strand, within Rru_A1475 at 1740.973 kb on + strand, within Rru_A1475 at 1740.973 kb on + strand, within Rru_A1475 at 1740.974 kb on - strand, within Rru_A1475 at 1740.974 kb on - strand, within Rru_A1475 at 1740.976 kb on + strand, within Rru_A1475 at 1740.976 kb on + strand, within Rru_A1475 at 1740.977 kb on - strand, within Rru_A1475 at 1740.977 kb on - strand, within Rru_A1475 at 1741.213 kb on + strand, within Rru_A1475 at 1741.444 kb on + strand, within Rru_A1475 at 1741.445 kb on - strand, within Rru_A1475 at 1741.445 kb on - strand, within Rru_A1475 at 1742.280 kb on + strand at 1742.357 kb on - strand at 1742.434 kb on + strand, within Rru_A1476 at 1742.435 kb on - strand, within Rru_A1476 at 1742.670 kb on + strand, within Rru_A1476 at 1742.670 kb on + strand, within Rru_A1476 at 1742.670 kb on + strand, within Rru_A1476 at 1742.670 kb on + strand, within Rru_A1476 at 1742.670 kb on + strand, within Rru_A1476 at 1742.670 kb on + strand, within Rru_A1476 at 1742.671 kb on - strand, within Rru_A1476 at 1742.671 kb on - strand, within Rru_A1476 at 1742.671 kb on - strand, within Rru_A1476 at 1742.671 kb on - strand, within Rru_A1476 at 1742.829 kb on - strand at 1742.943 kb on + strand at 1742.943 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Valeric 25 mM; anaerobic remove 1,739,841 + Rru_A1474 0.39 +0.8 1,739,842 - Rru_A1474 0.39 +0.4 1,739,865 + Rru_A1474 0.41 +0.4 1,739,866 - Rru_A1474 0.41 +0.3 1,740,018 + Rru_A1474 0.52 -0.2 1,740,019 - Rru_A1474 0.52 -1.6 1,740,216 + Rru_A1474 0.67 +0.5 1,740,216 + Rru_A1474 0.67 -2.9 1,740,489 + Rru_A1474 0.87 -0.0 1,740,489 + Rru_A1474 0.87 -0.2 1,740,489 + Rru_A1474 0.87 -0.4 1,740,490 - Rru_A1474 0.87 -0.9 1,740,490 - Rru_A1474 0.87 +2.3 1,740,624 + +0.2 1,740,658 + -0.1 1,740,658 + -0.2 1,740,659 - -1.3 1,740,665 + -0.5 1,740,686 + +0.4 1,740,718 + +1.1 1,740,718 + -0.5 1,740,718 + +0.1 1,740,719 - -3.2 1,740,719 - -0.2 1,740,719 - -2.9 1,740,781 + -1.8 1,740,781 + +1.5 1,740,782 - -0.8 1,740,973 + Rru_A1475 0.20 -3.9 1,740,973 + Rru_A1475 0.20 +0.5 1,740,973 + Rru_A1475 0.20 -0.3 1,740,974 - Rru_A1475 0.20 -1.4 1,740,974 - Rru_A1475 0.20 -2.6 1,740,976 + Rru_A1475 0.20 +0.8 1,740,976 + Rru_A1475 0.20 -0.1 1,740,977 - Rru_A1475 0.20 -0.4 1,740,977 - Rru_A1475 0.20 -2.6 1,741,213 + Rru_A1475 0.38 +1.4 1,741,444 + Rru_A1475 0.56 -0.4 1,741,445 - Rru_A1475 0.56 -1.5 1,741,445 - Rru_A1475 0.56 -0.5 1,742,280 + +0.3 1,742,357 - -0.2 1,742,434 + Rru_A1476 0.13 -0.1 1,742,435 - Rru_A1476 0.13 -1.2 1,742,670 + Rru_A1476 0.80 -0.0 1,742,670 + Rru_A1476 0.80 +0.1 1,742,670 + Rru_A1476 0.80 +0.2 1,742,670 + Rru_A1476 0.80 -1.7 1,742,670 + Rru_A1476 0.80 -0.2 1,742,670 + Rru_A1476 0.80 +1.4 1,742,671 - Rru_A1476 0.80 -0.9 1,742,671 - Rru_A1476 0.80 +1.0 1,742,671 - Rru_A1476 0.80 -1.5 1,742,671 - Rru_A1476 0.80 -0.2 1,742,829 - -0.3 1,742,943 + -0.2 1,742,943 + -0.6
Or see this region's nucleotide sequence