Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35425 and RR42_RS35430 are separated by 585 nucleotides RR42_RS35430 and RR42_RS35435 are separated by 680 nucleotides
RR42_RS35425: RR42_RS35425 - aspartate carbamoyltransferase, at 3,400,420 to 3,400,941
_RS35425
RR42_RS35430: RR42_RS35430 - hypothetical protein, at 3,401,527 to 3,401,907
_RS35430
RR42_RS35435: RR42_RS35435 - NAD(FAD)-dependent dehydrogenase, at 3,402,588 to 3,404,255
_RS35435
Position (kb)
3401
3402 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3400.543 kb on - strand, within RR42_RS35425 at 3400.601 kb on + strand, within RR42_RS35425 at 3400.777 kb on + strand, within RR42_RS35425 at 3400.778 kb on - strand, within RR42_RS35425 at 3400.810 kb on + strand, within RR42_RS35425 at 3400.810 kb on + strand, within RR42_RS35425 at 3400.929 kb on + strand at 3401.026 kb on + strand at 3401.026 kb on + strand at 3401.065 kb on - strand at 3401.120 kb on + strand at 3401.225 kb on + strand at 3401.225 kb on + strand at 3401.225 kb on + strand at 3401.226 kb on - strand at 3401.234 kb on + strand at 3401.234 kb on + strand at 3401.235 kb on - strand at 3401.235 kb on - strand at 3401.270 kb on - strand at 3401.279 kb on - strand at 3401.486 kb on + strand at 3401.487 kb on - strand at 3401.534 kb on + strand at 3401.534 kb on + strand at 3401.535 kb on - strand at 3401.535 kb on - strand at 3401.545 kb on + strand at 3401.594 kb on - strand, within RR42_RS35430 at 3401.594 kb on - strand, within RR42_RS35430 at 3401.622 kb on + strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.623 kb on - strand, within RR42_RS35430 at 3401.625 kb on + strand, within RR42_RS35430 at 3401.625 kb on + strand, within RR42_RS35430 at 3401.626 kb on - strand, within RR42_RS35430 at 3401.714 kb on + strand, within RR42_RS35430 at 3401.714 kb on + strand, within RR42_RS35430 at 3401.718 kb on + strand, within RR42_RS35430 at 3401.719 kb on - strand, within RR42_RS35430 at 3401.719 kb on - strand, within RR42_RS35430 at 3401.764 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.780 kb on + strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.781 kb on - strand, within RR42_RS35430 at 3401.831 kb on - strand, within RR42_RS35430 at 3401.859 kb on - strand, within RR42_RS35430 at 3401.859 kb on - strand, within RR42_RS35430 at 3401.871 kb on + strand at 3401.872 kb on - strand at 3402.155 kb on - strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.221 kb on + strand at 3402.222 kb on - strand at 3402.222 kb on - strand at 3402.222 kb on - strand at 3402.225 kb on + strand at 3402.226 kb on - strand at 3402.226 kb on - strand at 3402.326 kb on + strand at 3402.326 kb on + strand at 3402.327 kb on - strand at 3402.349 kb on - strand at 3402.381 kb on + strand at 3402.382 kb on - strand at 3402.382 kb on - strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.443 kb on + strand at 3402.445 kb on + strand at 3402.445 kb on + strand at 3402.445 kb on + strand at 3402.446 kb on - strand at 3402.446 kb on - strand at 3402.446 kb on - strand at 3402.449 kb on + strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.450 kb on - strand at 3402.564 kb on + strand at 3402.564 kb on + strand at 3402.565 kb on - strand at 3402.577 kb on + strand at 3402.578 kb on - strand at 3402.590 kb on - strand at 3402.729 kb on - strand at 3402.729 kb on - strand at 3402.748 kb on + strand at 3402.815 kb on - strand, within RR42_RS35435 at 3402.835 kb on + strand, within RR42_RS35435
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,400,543 - RR42_RS35425 0.24 -0.3 3,400,601 + RR42_RS35425 0.35 +0.1 3,400,777 + RR42_RS35425 0.68 -1.0 3,400,778 - RR42_RS35425 0.69 -0.5 3,400,810 + RR42_RS35425 0.75 -0.3 3,400,810 + RR42_RS35425 0.75 -0.1 3,400,929 + -0.5 3,401,026 + -0.6 3,401,026 + +0.4 3,401,065 - -0.1 3,401,120 + +0.1 3,401,225 + +0.2 3,401,225 + -0.2 3,401,225 + -1.1 3,401,226 - +1.2 3,401,234 + -0.4 3,401,234 + +1.5 3,401,235 - -0.8 3,401,235 - +0.1 3,401,270 - +0.3 3,401,279 - -1.1 3,401,486 + +0.4 3,401,487 - +0.7 3,401,534 + -2.1 3,401,534 + +0.2 3,401,535 - -0.2 3,401,535 - -0.1 3,401,545 + +0.1 3,401,594 - RR42_RS35430 0.18 -0.6 3,401,594 - RR42_RS35430 0.18 +0.3 3,401,622 + RR42_RS35430 0.25 +0.7 3,401,623 - RR42_RS35430 0.25 +0.5 3,401,623 - RR42_RS35430 0.25 +0.1 3,401,623 - RR42_RS35430 0.25 +0.4 3,401,625 + RR42_RS35430 0.26 -2.0 3,401,625 + RR42_RS35430 0.26 +0.6 3,401,626 - RR42_RS35430 0.26 +0.7 3,401,714 + RR42_RS35430 0.49 -0.2 3,401,714 + RR42_RS35430 0.49 -1.4 3,401,718 + RR42_RS35430 0.50 +0.4 3,401,719 - RR42_RS35430 0.50 -0.8 3,401,719 - RR42_RS35430 0.50 -2.2 3,401,764 + RR42_RS35430 0.62 -0.6 3,401,780 + RR42_RS35430 0.66 -1.2 3,401,780 + RR42_RS35430 0.66 +0.8 3,401,780 + RR42_RS35430 0.66 -0.1 3,401,780 + RR42_RS35430 0.66 -0.5 3,401,781 - RR42_RS35430 0.67 +0.8 3,401,781 - RR42_RS35430 0.67 -0.3 3,401,781 - RR42_RS35430 0.67 -1.0 3,401,831 - RR42_RS35430 0.80 +0.7 3,401,859 - RR42_RS35430 0.87 +0.6 3,401,859 - RR42_RS35430 0.87 +0.4 3,401,871 + +0.5 3,401,872 - +1.1 3,402,155 - +0.2 3,402,221 + +1.3 3,402,221 + -0.7 3,402,221 + +0.0 3,402,221 + +0.0 3,402,221 + +0.3 3,402,222 - +0.6 3,402,222 - +0.5 3,402,222 - +0.0 3,402,225 + -0.4 3,402,226 - +0.1 3,402,226 - -0.0 3,402,326 + -0.2 3,402,326 + +0.3 3,402,327 - -0.3 3,402,349 - +1.6 3,402,381 + +0.5 3,402,382 - -0.3 3,402,382 - -0.3 3,402,443 + -0.1 3,402,443 + +0.5 3,402,443 + -0.3 3,402,443 + -0.4 3,402,445 + -0.9 3,402,445 + -0.4 3,402,445 + +1.8 3,402,446 - +1.2 3,402,446 - +1.1 3,402,446 - +0.9 3,402,449 + -1.5 3,402,450 - +0.8 3,402,450 - +0.3 3,402,450 - -1.4 3,402,450 - -0.5 3,402,450 - -1.1 3,402,450 - +0.0 3,402,564 + +0.1 3,402,564 + -0.7 3,402,565 - +0.5 3,402,577 + +0.4 3,402,578 - +0.9 3,402,590 - +0.9 3,402,729 - +0.2 3,402,729 - +0.0 3,402,748 + +0.1 3,402,815 - RR42_RS35435 0.14 -0.2 3,402,835 + RR42_RS35435 0.15 -1.6
Or see this region's nucleotide sequence