Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS34225

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS34220 and RR42_RS34225 are separated by 238 nucleotidesRR42_RS34225 and RR42_RS34230 are separated by 132 nucleotides RR42_RS34220: RR42_RS34220 - pyruvate dehydrogenase, at 3,101,347 to 3,104,022 _RS34220 RR42_RS34225: RR42_RS34225 - methylmalonate-semialdehyde dehydrogenase, at 3,104,261 to 3,105,781 _RS34225 RR42_RS34230: RR42_RS34230 - LysR family transcriptional regulator, at 3,105,914 to 3,106,849 _RS34230 Position (kb) 3104 3105 3106Strain fitness (log2 ratio) -2 -1 0 1at 3103.382 kb on + strand, within RR42_RS34220at 3103.383 kb on - strand, within RR42_RS34220at 3103.383 kb on - strand, within RR42_RS34220at 3103.461 kb on - strand, within RR42_RS34220at 3103.496 kb on + strand, within RR42_RS34220at 3103.661 kb on + strand, within RR42_RS34220at 3103.661 kb on + strand, within RR42_RS34220at 3103.661 kb on + strand, within RR42_RS34220at 3103.662 kb on - strand, within RR42_RS34220at 3103.662 kb on - strand, within RR42_RS34220at 3103.662 kb on - strand, within RR42_RS34220at 3103.662 kb on - strand, within RR42_RS34220at 3103.662 kb on - strand, within RR42_RS34220at 3103.796 kb on - strandat 3103.796 kb on - strandat 3103.823 kb on + strandat 3104.052 kb on - strandat 3104.052 kb on - strandat 3104.061 kb on + strandat 3104.121 kb on + strandat 3104.121 kb on + strandat 3104.199 kb on + strandat 3104.262 kb on + strandat 3104.262 kb on + strandat 3104.263 kb on - strandat 3104.273 kb on - strandat 3104.367 kb on + strandat 3104.368 kb on - strandat 3104.437 kb on + strand, within RR42_RS34225at 3104.512 kb on - strand, within RR42_RS34225at 3104.619 kb on + strand, within RR42_RS34225at 3104.620 kb on - strand, within RR42_RS34225at 3104.620 kb on - strand, within RR42_RS34225at 3104.620 kb on - strand, within RR42_RS34225at 3104.751 kb on + strand, within RR42_RS34225at 3104.751 kb on + strand, within RR42_RS34225at 3104.752 kb on - strand, within RR42_RS34225at 3105.060 kb on + strand, within RR42_RS34225at 3105.061 kb on - strand, within RR42_RS34225at 3105.387 kb on + strand, within RR42_RS34225at 3105.413 kb on - strand, within RR42_RS34225at 3105.413 kb on - strand, within RR42_RS34225at 3105.736 kb on - strandat 3105.844 kb on - strandat 3105.844 kb on - strandat 3105.986 kb on + strandat 3106.390 kb on + strand, within RR42_RS34230at 3106.404 kb on + strand, within RR42_RS34230at 3106.442 kb on + strand, within RR42_RS34230at 3106.443 kb on - strand, within RR42_RS34230at 3106.506 kb on - strand, within RR42_RS34230at 3106.506 kb on - strand, within RR42_RS34230

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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3,103,382 + RR42_RS34220 0.76 -0.0
3,103,383 - RR42_RS34220 0.76 -0.5
3,103,383 - RR42_RS34220 0.76 +0.2
3,103,461 - RR42_RS34220 0.79 -0.1
3,103,496 + RR42_RS34220 0.80 -1.0
3,103,661 + RR42_RS34220 0.86 -0.7
3,103,661 + RR42_RS34220 0.86 +1.0
3,103,661 + RR42_RS34220 0.86 +0.2
3,103,662 - RR42_RS34220 0.87 -0.3
3,103,662 - RR42_RS34220 0.87 +0.5
3,103,662 - RR42_RS34220 0.87 +0.3
3,103,662 - RR42_RS34220 0.87 +1.5
3,103,662 - RR42_RS34220 0.87 +0.7
3,103,796 - +1.7
3,103,796 - -0.2
3,103,823 + -0.4
3,104,052 - +1.1
3,104,052 - +0.6
3,104,061 + -0.3
3,104,121 + -0.2
3,104,121 + +0.5
3,104,199 + -1.5
3,104,262 + +1.4
3,104,262 + +0.2
3,104,263 - -0.7
3,104,273 - -0.2
3,104,367 + -1.2
3,104,368 - -1.0
3,104,437 + RR42_RS34225 0.12 +0.0
3,104,512 - RR42_RS34225 0.17 -0.8
3,104,619 + RR42_RS34225 0.24 -0.8
3,104,620 - RR42_RS34225 0.24 -0.1
3,104,620 - RR42_RS34225 0.24 +1.0
3,104,620 - RR42_RS34225 0.24 +0.1
3,104,751 + RR42_RS34225 0.32 +0.6
3,104,751 + RR42_RS34225 0.32 +0.7
3,104,752 - RR42_RS34225 0.32 -0.1
3,105,060 + RR42_RS34225 0.53 +0.0
3,105,061 - RR42_RS34225 0.53 +0.0
3,105,387 + RR42_RS34225 0.74 -2.5
3,105,413 - RR42_RS34225 0.76 +0.1
3,105,413 - RR42_RS34225 0.76 -0.5
3,105,736 - -0.1
3,105,844 - +0.4
3,105,844 - +0.9
3,105,986 + -0.2
3,106,390 + RR42_RS34230 0.51 -0.2
3,106,404 + RR42_RS34230 0.52 -0.5
3,106,442 + RR42_RS34230 0.56 +0.5
3,106,443 - RR42_RS34230 0.57 -0.9
3,106,506 - RR42_RS34230 0.63 -0.7
3,106,506 - RR42_RS34230 0.63 -0.1

Or see this region's nucleotide sequence