Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS34220 and RR42_RS34225 are separated by 238 nucleotides RR42_RS34225 and RR42_RS34230 are separated by 132 nucleotides
RR42_RS34220: RR42_RS34220 - pyruvate dehydrogenase, at 3,101,347 to 3,104,022
_RS34220
RR42_RS34225: RR42_RS34225 - methylmalonate-semialdehyde dehydrogenase, at 3,104,261 to 3,105,781
_RS34225
RR42_RS34230: RR42_RS34230 - LysR family transcriptional regulator, at 3,105,914 to 3,106,849
_RS34230
Position (kb)
3104
3105
3106 Strain fitness (log2 ratio)
-2
-1
0
1 at 3103.382 kb on + strand, within RR42_RS34220 at 3103.383 kb on - strand, within RR42_RS34220 at 3103.383 kb on - strand, within RR42_RS34220 at 3103.461 kb on - strand, within RR42_RS34220 at 3103.496 kb on + strand, within RR42_RS34220 at 3103.661 kb on + strand, within RR42_RS34220 at 3103.661 kb on + strand, within RR42_RS34220 at 3103.661 kb on + strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.662 kb on - strand, within RR42_RS34220 at 3103.796 kb on - strand at 3103.796 kb on - strand at 3103.823 kb on + strand at 3104.052 kb on - strand at 3104.052 kb on - strand at 3104.061 kb on + strand at 3104.121 kb on + strand at 3104.121 kb on + strand at 3104.199 kb on + strand at 3104.262 kb on + strand at 3104.262 kb on + strand at 3104.263 kb on - strand at 3104.273 kb on - strand at 3104.367 kb on + strand at 3104.368 kb on - strand at 3104.437 kb on + strand, within RR42_RS34225 at 3104.512 kb on - strand, within RR42_RS34225 at 3104.619 kb on + strand, within RR42_RS34225 at 3104.620 kb on - strand, within RR42_RS34225 at 3104.620 kb on - strand, within RR42_RS34225 at 3104.620 kb on - strand, within RR42_RS34225 at 3104.751 kb on + strand, within RR42_RS34225 at 3104.751 kb on + strand, within RR42_RS34225 at 3104.752 kb on - strand, within RR42_RS34225 at 3105.060 kb on + strand, within RR42_RS34225 at 3105.061 kb on - strand, within RR42_RS34225 at 3105.387 kb on + strand, within RR42_RS34225 at 3105.413 kb on - strand, within RR42_RS34225 at 3105.413 kb on - strand, within RR42_RS34225 at 3105.736 kb on - strand at 3105.844 kb on - strand at 3105.844 kb on - strand at 3105.986 kb on + strand at 3106.390 kb on + strand, within RR42_RS34230 at 3106.404 kb on + strand, within RR42_RS34230 at 3106.442 kb on + strand, within RR42_RS34230 at 3106.443 kb on - strand, within RR42_RS34230 at 3106.506 kb on - strand, within RR42_RS34230 at 3106.506 kb on - strand, within RR42_RS34230
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,103,382 + RR42_RS34220 0.76 -0.0 3,103,383 - RR42_RS34220 0.76 -0.5 3,103,383 - RR42_RS34220 0.76 +0.2 3,103,461 - RR42_RS34220 0.79 -0.1 3,103,496 + RR42_RS34220 0.80 -1.0 3,103,661 + RR42_RS34220 0.86 -0.7 3,103,661 + RR42_RS34220 0.86 +1.0 3,103,661 + RR42_RS34220 0.86 +0.2 3,103,662 - RR42_RS34220 0.87 -0.3 3,103,662 - RR42_RS34220 0.87 +0.5 3,103,662 - RR42_RS34220 0.87 +0.3 3,103,662 - RR42_RS34220 0.87 +1.5 3,103,662 - RR42_RS34220 0.87 +0.7 3,103,796 - +1.7 3,103,796 - -0.2 3,103,823 + -0.4 3,104,052 - +1.1 3,104,052 - +0.6 3,104,061 + -0.3 3,104,121 + -0.2 3,104,121 + +0.5 3,104,199 + -1.5 3,104,262 + +1.4 3,104,262 + +0.2 3,104,263 - -0.7 3,104,273 - -0.2 3,104,367 + -1.2 3,104,368 - -1.0 3,104,437 + RR42_RS34225 0.12 +0.0 3,104,512 - RR42_RS34225 0.17 -0.8 3,104,619 + RR42_RS34225 0.24 -0.8 3,104,620 - RR42_RS34225 0.24 -0.1 3,104,620 - RR42_RS34225 0.24 +1.0 3,104,620 - RR42_RS34225 0.24 +0.1 3,104,751 + RR42_RS34225 0.32 +0.6 3,104,751 + RR42_RS34225 0.32 +0.7 3,104,752 - RR42_RS34225 0.32 -0.1 3,105,060 + RR42_RS34225 0.53 +0.0 3,105,061 - RR42_RS34225 0.53 +0.0 3,105,387 + RR42_RS34225 0.74 -2.5 3,105,413 - RR42_RS34225 0.76 +0.1 3,105,413 - RR42_RS34225 0.76 -0.5 3,105,736 - -0.1 3,105,844 - +0.4 3,105,844 - +0.9 3,105,986 + -0.2 3,106,390 + RR42_RS34230 0.51 -0.2 3,106,404 + RR42_RS34230 0.52 -0.5 3,106,442 + RR42_RS34230 0.56 +0.5 3,106,443 - RR42_RS34230 0.57 -0.9 3,106,506 - RR42_RS34230 0.63 -0.7 3,106,506 - RR42_RS34230 0.63 -0.1
Or see this region's nucleotide sequence